• The Korean Journal of Food And Nutrition
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• v.28 no.4
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• pp.527-532
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• 2015

This study was performed to investigate the phyico-chemical characteristics of breast meat from spent hens (SP) in comparison with the same part from the broilers (BR). The moisture and crude protein contents for SP were 72.56% and 24.26%, which were lower than 75.87% and 24.64% for BR. Crude fat and crude ash contents in SP were 0.45% and 1.00%, which were higher than the BR contents of 0.41% and 0.51%. The respective L, a and b value for SP were 48.61, 2.40 and 2.42, which were lower than the BR values of 49.41, 1.45 and 3.06 respectively. The pH for SP was 5.89, which was higher than pH 5.79 for BR. The WHC for SP was 50.29%, which was lower than that of BR at 62.31%. SP scored 28.04% in the heating loss test, which was significantly higher than 19.09% for BR. The shear forces for SP and BR were 4.86 kg and 1.36 kg respectively, which meant that the texture of SP was much tougher than that of BR. Hardness for SP was 8.89 kg while that of BR was 3.92 kg indicating that the SP texture is firmer than that of BR. Oleic acid was most abundant fatty acid in both samples and was at 44.15% in SP and 27.68% in BR.

• Journal of Microbiology and Biotechnology
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• v.22 no.10
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• pp.1423-1431
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• 2012

The inhibitory effect of IgY against Vibrio parahaemolyticus and Vibrio vulnificus responsible for seafood-borne diseases was investigated in this study. Water-soluble fractions (WSF) of protein containing IgYs were isolated from the egg yolk of hens initially immunized with formalin-inactivated V. parahaemolyticus or V. vulnificus. Protein, total and specific IgY contents of the WSF were determined. The inhibitory and protective effects of IgYs on the growth of V. parahaemolyticus and V. vulnificus were assayed in liquid medium and in mice. IgYs showed high affinity to their corresponding antigens with high titer from day 28 onwards. Protein contents and total IgY concentrations remained stable throughout the immunization period, whereas specific IgY concentrations increased steadily and reached a plateau at day 49. Specific IgY powder (150 mg/ml) significantly inhibited further multiplication of both V. parahaemolyticus and V. vulnificus in liquid medium as compared with the control IgY. The bacteria count in mice feces was lower in mice pretreated with specific IgYs than in those pretreated with PBS or control IgY. Higher survival of mice was observed in the experimental groups pretreated with either anti-V. parahaemolyticus (75% survival) or anti-V. vulnificus (87% survival) IgYs, compared with those in the control groups pretreated with PBS or nonspecific IgY. All mice in the control groups died within three days after bacteria inoculation; hence, the protective effect of specific IgYs against infection caused by V. parahaemolyticus and V. vulnificus was demonstrated.

• Korean Journal of Animal Reproduction
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• v.16 no.2
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• pp.87-102
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• 1992

A rapid, sensitive, and specific enzyme-linked immuno-sorbent assay (ELISA) for bovine casein was developed. Biotinylated casein and peroxidase-conjugated avidin were used in the assay with antibody separated from yolks of immunized hens. Caseins were biotinylated with sulfo-N-hydroxy succinimido biotin and peroxi-dase-conjugated avidin bound the biotinylated casein which became bound to immobilized anti-body on a microplate. The antibodies were specific for bovine $\alpha$- and $\beta$-caseins, and their cross-reactivities with whey proteins, IgG, and serum albumin from bovine were not detectable by ELISA and Western blot. Various sensitivities ranging from 2ng/ml to 20${\mu}\textrm{g}$/ml of casein were achieved, and were controlled by adding vanous concentrations of the biotinylated casein. Parallelism was observed between standard and sample curves. The coefficients of variation of intra-assays and inter-assays from the most sensitive assay were 5.5 and 5.7%, respectively, at the 50% displacement. Casein contents of peripaturient milk samples showed that casein secretion rapidly increased 3d prepartum.

• Korean Journal of Poultry Science
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• v.16 no.3
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• pp.169-174
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• 1989

Culled laying hens used in this study were Arbor acres, which reared to S 35 days old on commerical formula feed for chicken. Liver, gizzard, breast and thigh muscles separated from each carcass, and total lipid was extracted and fractionated to neutral, phospho and glycolipid and then fatty acid composition were analyzed. Liver had the highest level of. total lipid, and breast tissue had the least among tissues tested. The neutral, phospho and glycolipid contents of total lipid had more thigh, breast and gizzard than other tissues, respectively. The major fatty acid in total and neutral lipid were palmitic, stearic, oleic and linoleic acid. And the major fatty acid in phospholipid was palmitic, stearic, oleic, linoleic, arachidonic and docosahexaenoic acid, and palmitic, palmitoleic, stearic, oleic and linoleic acid in case of glycolipid. The fatty acid contents of neutral, phospho and glycolipid in total lipid had more oleic, docosahexaenoic and linoleic acid than other lipid, respectively. Contents of unsaturated fatty acid of total and neutral lipid were comparatively high in thigh, and phospho and glycolipid were high in breast and liver, respectively. Contents of Polyunsaturated fatty acids were comparatively high in phospholipids than other lipids.

• Korean Journal of Poultry Science
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• v.25 no.4
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• pp.161-167
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• 1998

This experiment was carried out to develope the production of specific yolk antibody from laying hens immunized with antigens from Salmonella enteritidis. Antigenic protein isolated from the flagella of Salmonella enteritidis, determined by SDS-PAGE, was pure and has a molecular mass of approximately 54.6 kDa. It was observed that the antibody titers both in egg yolk and serum were performed at 2 weeks after immunization with flagella antigen to the laying hen. And the level was increased gradually to 6 weeks after immunization. At the time of 6 weeks, the antibody titer of yolk showed higher than that of serum. According to the results of specificity test(ELISA), the yolk antibody did not react with different bacterial strains(S. choleraesuis, ETEC Kl2:K99, K88,987P), but reacted only with S. enteritidis strain. The contents of immunoglobulin(IgY) in an egg yolk was 106mg approximately. By the isolation procedure of IgY from the egg yolk, 88.3 percent of IgY content was recovered in this study.

• Korean Journal of Poultry Science
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• v.23 no.1
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• pp.39-46
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• 1996

An experiment was conducted to investigate the effect of feeding split diets for a.m. and p.m. in laying hens on egg shell qualities, and to study the relationship among the methods of measuring shell quality with 480 ISA Brown layers during 32∼36, 52∼56 and 72∼76 wk of age, respectively. A total of 1,080 eggs were collected from 6 treatment groups in every laying period and weighed individually. Egg specific gravity, egg breaking strength, shell thickness and shell percentage of eggs were measured, and then correlation coefficients (r) and regression equations were calculated. By feeding the split diets for a.m. and p.m. differing in ME, CP, and Ca levels, and as the Ca level of the p. m. diet increased, eggshell qualities were improved(P<0.05). The correlation coefficient values indicate that shell percentage, shell thickness and egg specific gravity are highly correlated with the egg breaking strength(P<0.01).

• Korean Journal of Poultry Science
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• v.30 no.2
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• pp.91-94
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• 2003

Artificial insemination of cryopreserved semen is not practiced in broiler breeder industry although its benefit has been recognized for years. Thus, the present study explored the possibility of artificial insemination with cryopreserved semen in Ross broiler breeders. Sixty Ross female broiler breeders were artificially inseminated either with fresh or with cryopreserved semen pooled from five male Ross broiler breeders. On.e hundred eighty eggs were collected from each semen treatment and hatched. Fertility and hatchability of fertilized eggs collected from hens artificially inseminated with cryopreserved semen were 68.0 and 47.1％, respectively, compared to 88.3 and 87.2％ with fresh semen. Both unfertilized eggs and dead embryos during hatch were significantly (P<0.05) higher in the cryopreserved semen­treated group by approximately 2.7­ and 3.2­fold than the fresh semen­treated counterparts. the moderately low fertility and markedly low hatchability of fertilized eggs obtained from cryopreserved semen observed in this study may be of practical interests in broiler breeder industry and deserves further study.

• Korean Journal of Organic Agriculture
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• v.10 no.4
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• pp.47-60
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• 2002

'Probiotics' as a live microbial feed supplementation which beneficially affects the host animal by improving its microbial balance and it is known to as a substitue for antibiotics in livestock feed industry. Lactic acid bacteria as a Lactobacillus sp. is formed acid and decrease pH in gastro-intestine that is result in suppress harmful microorganism. Lactobacillus sp. also produces vitamin and a variety amino acids. Yeast as a saccharomyces sp. secretes digestive enzymes, decreases ammonia emission and increases feed palatability by alcohol and glutamic acid. The effects of dietary probiotics in monogastric animals that improve weight gain and feed efficiency ratio and decrease diarrhea accurence frequency in pigs. Also, probiotics increase egg production ratio and beneficial microorganisms in laying hens. In broiler, they have more gain weight and lower blood cholesterol concentrations by probiotics. However, the other study reported probiotics supplementation in animal diets has no effect on ADG, G/F or performance. Thus, future study in these area will allow for more efficient use of the probiotics, selection of more superior microorganism and development of more efficient environment-friendly probiotics like a photosynthetic bacteria.

• Korean Journal of Food Science and Technology
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• v.38 no.1
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• pp.93-98
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• 2006

Effects of anti-Helicobacter pylori IgY powder on H. pylori infection were evaluated 3 and 7 weeks after powder feeding by urease, PCR, and histological tests, and specific IgG assay of murine gastric tissue using mouse model. To produce anti-H. pylori IgY powder, laying hens were immunized with H. pylori prior to egg yolk harvest. C57BL/6 mice showing high response to H. pylori were infected with H. pylori and fed with the anti-H. pylori IgY powder. In urease and PCR tests, urease activity and gene count of anti-H. pylori IgY powder-fed group significantly decreased in comparison with control. Histological results indicated anti-H. pylori IgY powder effectively protected mice from H. pylori.

• Korean Journal of Agricultural Science
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• v.45 no.4
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• pp.687-694
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• 2018

This study was conducted to investigate the laying performance of six strains of Korean domestic chickens (KDC 1A, 2A, 3A, 1B, 2B and 3B). A total of 288 20-week-old KDC hens were allocated in a completely randomized design to give 12 replicates per each strain (4 chickens per pen). The chickens were fed commercial diets (i.e., week 20 - 32, crude protein [CP] 18.0% and metabolizable energy [ME] 2,850 kcal/kg; week 32 - 40, CP 17.0% and ME 2,800 kcal/kg) ad libitum together with fresh water during the 20-week experiment. Body weight, age of sexual maturity, egg weight, hen-day egg production (HDP) and feed conversion ratio (FCR) were measured during the experiment period (week 20 - 40). Our results show that strain 3B had an improved (p < 0.05) HDP and FCR compared with the other strains on week 24 - 28. However, strain 3A had an improved (p < 0.05) HDP and FCR compared to the other strains on week 28 - 40. Similarly, strain 3A had a higher egg weight (p < 0.05) compared to the other strains on week 28, 32 and 40. In conclusion, the higher laying performance of strain 3A indicates its potential in the development of a KDC strain with improved commercial laying performance.