• 한국신재생에너지학회:학술대회논문집
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• 2008.10a
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• pp.67-70
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• 2008

Biological conversion of biomass into fuels and chemicals requires hydrolysis of the polysaccharide fraction into monomeric sugars. Hydrolysis can be performed enzymatically, and with dilute or concentrate mineral acids. In this study, dilute sulfuric acid used as a catalyst for the hydrolysis of rapeseed straw. The purpose of this study is to optimize the hydrolysis process in a 15ml bomb tube reactor and investigate the effects of the acid concentration, temperature and reaction time on the hemicellulose removal and consequently on the production of sugars (xylose, glucose and arabinose) as well as on the formation of by-products (furfural, 5-hydroxymethylfurfural and acetic acid). Statistical analysis was based on a model composition corresponding to a $3^3$ orthogonal factorial design and employed the response surface methodology (RSM) to optimize the hydrolysis conditions, aiming to attain maximum xylose extraction from hemicellulose of rapeseed straw. The obtained optimum conditions were: acid concentration of 0.77%, temperature of $164^{\circ}C$ with a reaction time of 18min. Under these conditions, 75.94% of the total xylose was removed and the hydrolysate contained 0.65g $L^{-1}$ Glucose, 0.36g $L^{-1}$ Arabinose, 3.59g $L^{-1}$ Xylose, 0.51g $L^{-1}$ Furfural, 1.36g $L^{-1}$ Acetic acid, and 0.08g $L^{-1}$ 5-hydroxymethylfurfural.

• Journal of The Korean Society of Grassland and Forage Science
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• v.11 no.1
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• pp.60-67
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• 1991

This experiment was conducted to investigate the effects of starch addition on the fermentative quality and dry matter digestibility(DMD) of Kudzu(Puerari~ thzmbergii Bentham) silage. The herbages was ensiled by the conventional methods in small plastic silo of 7.5 liters with addition of starch of 0 %, 2 %, 4 74, 6 % and 8 %, respectivery. The samples of kudzu silage were separated into leaf and stem, and was determined the pH, organic acid and characteristics of fiber such as neutral detergent fiber(NDF), acid detergent liber(ADF) and acid detergent lignin(ADL). The DMD of leaf and stem silage were evaluated by pepsin-cellulase technique method. The energy values(tota1 digestible nutrients, TDN; digestible energy, DE; metaboliz;~bie energy. ME) were calculated by DMD. The results obtained were as follows: 1. The fiberous meterials(such as NDF, AIIF and ADL) of leaf and stem were decreased with increasing levels of starch(p

• Journal of the Korean Society of Clothing and Textiles
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• v.31 no.8
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• pp.1286-1296
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• 2007

Hanji, the korean traditional papers were mostly made from mulberry paper. But the production and demand of hanji have decreased rapidly because mulberry paper yields were insufficient and handworked hanji procedures were complicated. Recently, the researches on hanji were carried out to improve the properties of hanji. Kenaf fibers have been interested as a substitute resource of mulberry paper for hanji production. In this research, Kenai pulps were manufactured with removal methods of lignin or hemicellulose from kenaf fibers and paper mulberry pulps with traditional alkali methods. Kenaf papers, paper mulberry, and kenaf/paper mulberry mixed papers were manufactured with their pulps. The crystallinity, fiber length, color of the pulps and tensile strength, tear strength, water absorption of the papers were investigated. The results were as follow: The removal rates of lignin of chemical retted kenaf fibers with sodium chlorite reaction for 40 minutes were 70% and were higher than 40% of double retted fibers. Paper mulberry pulps has less lignin and hemicellulose than kenaf differently. The crystallinity of paper mulberry pulps were very low with 60%, but kenaf pulps were 90%. The chemical retted CR-40-1 pulps were similar with paper mulberry pulps on fiber length & fibrilation of fibers. Tensile strength of paper mulberry were higher than kenaf papers because of fibrilation of paper mulberry, but tear strength were lower. Tensile strength and tear strength were improved on kenaf/paper mulberry 30/70 mixed papers.

• Journal of the Korean Wood Science and Technology
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• v.37 no.3
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• pp.222-230
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• 2009

This study was to investigate the effect of alkali treatment for long kenaf fiber to improve fiber surface characterics by removal of wax, lignin and hemicellulose which affect adversely for matrix union. SEM observation was also studied to check out the interface adhesion improvement by the alkali pre-treatment. From the result, interface coherence increased by 3% alkali pre-treatment and reached a maximum by 5% alkali pre-treatment. However, the 3% the bio-composites treated with 3% alkali was highest tensile and flexural strength than other.

• Korean Journal of Food Science and Technology
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• v.26 no.5
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• pp.490-495
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• 1994

Enzymatic solubilization of tofu-residue was attempted using carbohydrase isolated from Aspergillus niger CF-34. Tofu-residue, by-product of tofu manufacture or soymilk processing was used as the model for plant cell wall. It was found that tofu-residue was rich in nurients: 46.7% carbohydrate, 32.8% protein, the rest being lipid and ashes. Carbohydrate component of tofu-residue consisted of 36.8% cellulose and 62.6% hemicellulose. The carbohydrase was found to consist of pectinase, xylanase, PGase, CMCase, and SFase when tofu-residue and pectin were used as the carbon source. Enzyme induction was maximum at 7days of culture. Optimum reaction pH was 4.0, temperature $50^{\circ}C$. The enzyme was stable to $50^{\circ}C$, above which the stability decreased rapidly.

• The Korean Journal of Mycology
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• v.22 no.3
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• pp.254-259
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• 1994

The ever increasing demand for energy and the shortage of resources all over the world have generated interest in recycling renewable sources such as lignocelluloses which otherwise would go to waste and cause environmental pollution. Lignin is the incrustation material for cellulose and hemicellulose, therefore, cellulose and hemicellulose are not easily degraded. Recycling lignocellulosic wastes as agricultural material are benefit to everybody and everything. In order to improve ligninase and laccase production of Phanerochaete chrysosporium, BKM-F-1767 and Ceriporiopsis subvermispora, FP 90031-SP, were compared. The ligninase activity of P. chrysosporium was maximum on day 4.5 of shaking culture at 150 rpm 2.5 cm in a back and forth cycle. The laccase activity of C. subvermispora was maximum on day 5.5 for 2% malt extract+0.1% yeast extract+0.1% Tween 20+6 mM Benzyl alcohol culture medium at stationary state.

• Journal of Microbiology and Biotechnology
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• v.27 no.9
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• pp.1670-1680
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• 2017

Lignocellulose, composed mostly of cellulose, hemicellulose, and lignin generated through secondary growth of woody plant, is considered as promising resources for biofuel. In order to use lignocellulose as a biofuel, biodegradation besides high-cost chemical treatments were applied, but knowledge on the decomposition of lignocellulose occurring in a natural environment is insufficient. We analyzed the 16S rRNA gene and metagenome to understand how the lignocellulose is decomposed naturally in decayed Torreya nucifera (L) of Bija forest (Bijarim) in Gotjawal, an ecologically distinct environment. A total of 464,360 reads were obtained from 16S rRNA gene sequencing, representing diverse phyla; Proteobacteria (51%), Bacteroidetes (11%) and Actinobacteria (10%). The metagenome analysis using single molecules real-time sequencing revealed that the assembled contigs determined originated from Proteobacteria (58%) and Actinobacteria (10.3%). Carbohydrate Active enZYmes (CAZy)- and Protein families (Pfam)-based analysis showed that Proteobacteria was involved in degrading whole lignocellulose, and Actinobacteria played a role only in a part of hemicellulose degradation. Combining these results, it suggested that Proteobacteria and Actinobacteria had selective biodegradation potential for different lignocellulose substrates. Thus, it is considered that understanding of the systemic microbial degradation pathways may be a useful strategy for recycle of lignocellulosic biomass, and the microbial enzymes in Bija forest can be useful natural resources in industrial processes.

• Journal of the Korean Wood Science and Technology
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• v.21 no.3
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• pp.30-36
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• 1993

Wood chips of oak (Quercus mongolica) and larch (Larix leptolepis) were treated with a relatively low pressure steam(10~20 kg/$cm^2$) for 10~20 min (first-stage),and then increased pressure up to 30kg/$cm^2$ for 30 second (second-stage), and steam pressure was released intentionally to air. Main components of exploded wood were separated with 1% NaOH and hot water-methanol. In this work, the more effective low pressure explosion condition and separation method of wood main component were investigated. The results can be summarized as follows; 1. The yields of exploded wood were generally decreased with increasing steam pressure and reaction time. 2. The proper condition of steam explosion in low pressure for the separation of wood main components was 15kg/$cm^2$-10 min, in oak wood and 20kg/$cm^2$-10 min., then 30kg/$cm^2$-0.5 min, in larch wood. 3. The 23% of elude hemicellulose was obtained from the exploded oak wood which was treated with optimal condition. 4. In the case of hot water-methanol extraction, the ratio of delignification was 14~23% in the exploded larch wood and 42~55% in the exploded oak wood. 5. The methanol was more effective than 1% sodium hydroxide solution for extraction of lignin from exploded wood.

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.5
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• pp.852-856
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• 2004

This study was conducted to determine on the proximate analysis and the several structural carbohydrate for 11 kinds of new vegetables. The samples were dried at 6$0^{\circ}C$ for 24 hrs and ground to pass a 0.5 mm screen. The crude protein and crude fat contents of new vegetables were 2∼3 times higher than those of grain as dry matter basis. However the crude ash content of new vegetables was 7 times higher than that of grain. Total dietary fiber was ranged from 32.61% (Costamary) to 41,22% (Treviso) as dry matter basis. Insoluble dietary fiber was ranged from 21.58％ (Red leaf beet) to 28.95％ (Treviso) as dry matter basis. Soluble dietary fiber was ranged from 6.60% (Nakai) to 14.70% (Common danelion) as dry matter basis. Total carbohydrates was ranged from 73.62% (Salad bowl) to 36.30% (Red leaf beet) as dry matter basis. Neutral detergent fiber was ranged from 48.83% (Nakai) to 29.60% (Red leaf beet) as dry matter basis. Acid detergent lignin was ranged from 27.65% (Salad bowl) to 2.92% (Corn salad) as dry matter basis. Hemicellulose was ranged from 22.55% (Nakai) to 2.15% (Salad bowl).

• Korean Journal of Chemical Engineering
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• v.35 no.12
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• pp.2403-2412
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• 2018

The present study was aimed at evaluating the effect of variable sodium carbonate ($Na_2CO_3$) loading during wet air oxidation (WAO) pretreatment of rice straw in reducing biomass recalcitrance. The research study was intended to increase the cellulose recovery, hemicellulose solubilization, lignin removal in the solid fraction and limiting the generation of inhibitors in the liquid fraction while reducing the chemical input. The operating condition of $169^{\circ}C$, 4 bar, 18 min and 6.5 g/L $Na_2CO_3$ loading resulted in maximum cellulose recovery of 82.07% and hemicellulose solubilization and lignin removal of 85.43% and 65.42%, respectively, with a total phenolic content of 0.36 g/L in the liquid fraction. The crystallinity index increased from 47.69 to 51.25 along with enzymatic digestibility with an increase in $Na_2CO_3$ loading from 0 to 6.5 g/L as a result of removal of barriers for saccharification via effective cleavage of ether and ester bonds cross-linking the carbohydrates and lignin as indicated by FT-IR spectroscopy. A further increase in the $Na_2CO_3$ loading to 9.5 g/L did not significantly increase the sugar release. Thus, it was concluded that 6.5 g/L $Na_2CO_3$ during WAO is sufficient to increase the delignification and deacetylation, leading to significant changes in apparent cellulose crystallinity inter alia improvement in cellulose accessibility and digestibility of rice straw.