• 한국안광학회지
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• 제12권3호
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• pp.19-25
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• 2007

현재 시중에서 유통되고 있는 소프트 콘택트렌즈 관리용액인 다목적용액(Multi Purpose Solution, MPS)이 결막세포주(Clone 1-5C-4 cell line)에 미치는 증식저해정도와 가토안의 각막상피 및 내피조직에 미치는 손상정도를 비교 관찰하고자 시행하였다. MPS는 $ReNu^{(R)}$ (Baush & Lomb, USA), Opti-free $express^{(R)}$ (Alcon, USA), Free-sol $plus^{(R)}$ (Hanamedicon, Korea)를 사용하였다. 세포증식 저해율은 결막세포주를 배양 한 후 MTT assay로 검정하였고, 형태학적으로는 광학현미경으로 Hematoxylin & Eosin staining 표본을 제작하여 관찰하였다. In vivo 실험은 백색 가토 9마리(18안)를 3군으로 분류하여 실험군인 좌안(9안)에는 각 MPS제품을, 대조군인 우안(9안)에는 보존제가 포함되지 않은 멸균생리식염수를 접안하였다. 일정기간 접안 후, 가토안의 각막표변을 Rose bengal로 염색하여 관찰하였고 각막상피 및 내피조직의 변화는 주사전자현미경(Scanning Electron Microscopy, SEM)으로 비교 관찰하였다.

• 농업생명과학연구
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• 제50권3호
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• pp.129-139
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• 2016

Curcumin은 항산화제로서 신경세포의 보호작용에 관여하며, peroxiredoxin-5는 활성산소의 형성을 저해하여 산화적 스트레스로부터 신경세포를 보호한다고 알려져 있다. 본 연구는 허혈성 대뇌손상모델에서 curcumin에 의해 조절되는 peroxiredoxin-5 발현의 변화에 관하여 조사하였다. 실험동물은 흰쥐(Sprague-Dawley, 수컷)를 사용했으며, 허혈성 대뇌손상을 유도하기 위하여 중간대뇌동맥폐쇄술(MCAO)을 실시하였다. MCAO를 시행한 1시간 후에 curcumin(50mg/kg B.W.) 또는 vehicle을 복강으로 주사하였고, MCAO을 실시한 24시간 후 대뇌피질의 조직을 적출하였다. Hematoxylin과 eosin 조직염색 결과 MCAO를 시행한 대뇌피질에서는 신경세포의 괴사 소견을 보였지만, curcumin 투여군에서 이들 신경세포의 손상이 완화되어 있어 MCAO로 유도된 대뇌 손상시 curcumin의 보호효과를 확인하였다. 또한 MCAO를 실시한 vehicle+MCAO 실험군에서 peroxiredoxin-5 단백질의 발현은 감소하였으나, curcumin을 처리한 curcumin+MCAO 실험군에서는 vehicle+MCAO 실험군의 감소에 비해 감소의 폭이 현저히 줄어들어 MCAO를 시행하지 않은 sham군의 발현 수준으로 유지되었다. Reverse-transcription PCR과 Western blot 분석을 통해 중간대뇌동맥폐쇄술로 유도된 허혈성 대뇌손상 모델에서 peroxiredoxin-5 발현의 감소와 curcumin의 투여에 의한 peroxiredoxin-5 발현 감소의 완화를 확인하였다. 본 연구의 결과는 curcumin의 처리는 MCAO로 인한 peroxiredoxin-5 발현의 감소를 억제시킨다는 것을 보여주었다. 따라서, 대뇌손상 모델동물에서 curcumin은 MCAO로 유도된 peroxiredoxin-5 발현의 감소 정도를 완화시킴으로서 curcumin이 신경세포 보호작용에 기여하는 것으로 사료된다.

• 대한수의학회지
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• 제43권3호
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• pp.485-492
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• 2003

The pathological mechanism of impaired spermatogenesis after vasectomy has not been completely investigated. In this study, we examined pathological changes of the testis and the Fas-Fas ligand (FasL) mediated signaling pathway in apoptotic germ cell death after vasectomy in rats. Ten-weeks old Sprague-Dawley rats were underwent bilateral vasectomy and sacrificed after 1 day, 2 days, 3 days, 5 days, 1 week, 2 weeks, and 4 weeks of surgery and the testes were removed. Histopathological evaluation of spermatogenesis was performed by hematoxylin-eosin and periodic acid-Schiff-hematoxylin staining. To elucidate the pathophysiology of seminiferous tubule damage, terminal dUTP nick end labeling staining, electrophoresis assay of DNA fragmentation, and Western blotting analysis for Fas-FasL were performed. Relative weights of testes were decreased from 5 days after vasectomy. Germ cell degeneration were first found in the spermatogonia and spermatocytes at stages I-VI, and XII-XIV seminiferous tubules. Mean incidence of apoptotic germ cells after vasectomy progressively increased to peak in 5 days, and then gradually decreased to the control levels in 2 weeks after vasectomy. The expression of Fas-FasL reached maximum level at 5 days after vasectomy and then declined. In conclusion, impaired spermatogenesis after vasectomy associated with an increase in germ cell apoptasis, which is partly mediated by the activation of Fas-FasL.

• 한국어류학회지
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• 제29권4호
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• pp.252-257
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• 2017

잉어목 모래무지아과에 속하는 한국 고유종 돌마자 Microphysogobio yaluensis 난소 내 생식세포들의 형태학적 특징을 연구하기 광학현미경을 이용하여 조사하였다. 난자형성과정 (oogenesis)은 크게 염색인기 (chromatin-nucleolus stage), 주변인기(peri-nucleolus stage), 난황형성기(vitellogenesis)의 난황포 및 난황구기와 성숙(mature stage)의 단계로 구분되었다. 염색인기에는 배포가 크게 형성되며 실모양의 염색질이 산재되어 있다. 주변인기에는 핵 내에 산성의 인들이 핵막인근에 분포하고 있었으며, 난막(egg envelope)이 형성되기 시작하였다. 이후 난황형성기의 난황포 단계에서는 세포질의 대부분이 텅빈 공포모양의 난황포로 구성되며, 발생이 진행되면서 난황구 단계에서는 난황포 사이에 eosin에 염색되는 난황과립으로 대체되었다. 성숙단계에 도달한 난세포에는 많은 난황구들이 하나의 커다란 난황괴(yolk mass)를 형성하고 있었다. 이 시기의 난세포의 난막은 세포질과 여포세포층 사이에 얇게 형성되었다.

• The Korean Journal of Physiology and Pharmacology
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• 제5권4호
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• pp.353-357
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• 2001

The pathogenesis of the nasal polyp is multifactorial and choanal polyps can be defined by its origin of genesis: antrochoanal (maxillochoanal), ethmochoanal and sphenochoanal polyp. Transforming growth $factor-{\beta}\;(TGF-{\beta})$ has various biologic activities, including the regulation of epithelial proliferation, the promotion of extracellular matrix formation and the induction of angiogenesis, hence closely related to pathogenesis of nasal polyp. Twenty cases of choanal polyps (13 antrochoanal, 4 ethmochoanal and 3 sphenochoanal polyps) were included in this study. Each polyp was subdivided into its origin, pedicle and choanal part. Hematoxylin and eosin stain for routine histopathology and immunohistochemistry were employed to detect expression of $TGF-{\beta}1.$ According to polyp type, edematous type is common at origin part and fibrous type at choanal part, and showed no difference at pedicle part in frequency. In ethmochoanal and sphenochoanal polyps, glandulocystic and edematous type is more common than fibrous type. $TGF-{\beta}1$ was expressed in epithelial cells, endothelial cells, eosinophils and lymphocytes. There was no different expression of $TGF-{\beta}1$ in each kind of choanal polyps and separate parts in each polyp. But histologic finding of choanal polyp is different between origin, pedicle and choanal part. Also infiltration of inflammatory cells including eosinophils has no difference between origin site. The expression of $TGF-{\beta}1$ was observed at all the choanal polyps and no difference between origin site and each portions was noted.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제38권
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• pp.7.1-7.5
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• 2016

Background: This study examined the osteoinductive activity of demineralized dentin matrix (DDM) from human and polydeoxyribonucleotide (PDRN) for nude mice. Methods: Twenty healthy nude mice, weighing about 15~20 g, were used for the study. DDM from human and PDRN were prepared and implanted subcutaneously into the dorsal portion of the nude mice. The nude mice were sacrificed at 1, 2, and 4 weeks after grafting and evaluated histologically by hematoxylin-eosin and Masson's trichrome staining. The specimens were also evaluated via a histomorphometric study. Results: The DDM and PDRN induced new bone, osteoblasts, and fibroblasts in soft tissues. The histological findings showed bone-forming cells like osteoblasts and fibroblasts at 1, 2, and 4 weeks. New bone formation was observed in the histomorphometric study. In particular, the ratio of new bone formation was the highest at 2 weeks compared with the first week and fourth week. Conclusions: In this study, we showed that the PDRN used in this experimental model was able to induce bone regeneration when combined to the DDM.

• The Korean Journal of Physiology and Pharmacology
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• 제2권4호
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• pp.511-519
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• 1998

This study investigated the role of nitric oxide on the oxidative damage in gastric mucosa of rats which received ischemia/reperfusion and its relation to mucus. Nitric oxide synthesis modulators such as L-arginine and $N^G-nitro-L-arginine$ methyl ester, and sodium nitroprusside, a nitric oxide donor, were injected intraperitoneally to the rats 30 min prior to ischemia/reperfusion which was induced by clamping the celiac artery and the superior mesenteric artery for 30 min and reperfusion for 1 h. Lipid peroxide production, the contents of glutathione and mucus, and glutathione peroxidase activities of gastric mucosa were determined. Histological observation of gastric mucosa was performed by using hematoxylin-eosin staining and scanning electron microscopy. The result showed that ischemia/reperfusion increased lipid peroxide production and decreased the contents of glutathione and mucus as well as glutathione peroxidase activities of gastric mucosa. Ischemia/reperfusion induced gastric erosion and gross epithelial disruption of gastric mucosa. Pretreatment of L-arginine, a substrate for nitric oxide synthase, and sodium nitroprusside prevented ischemia/reperfusion-induced alterations of gastric mucosa. However, $N^G-nitro-$ L- arginine methyl ester, a nitric oxide synthase inhibitor, deteriorated oxidative damage induced by ischemia/reperfusion. In conclusion, nitric oxide has an antioxidant defensive role on gastric mucosa by maintaining mucus, glutathione, and glutathione peroxidase of gastric mucosa.

• Journal of Pharmaceutical Investigation
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• 제40권1호
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• pp.45-49
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• 2010

Unlike human, with some exceptions, animals do not heal with excessive scar. The lack of suitable animal model has hindered the development of effective scar therapy. We previously reported that partial thickness rabbit ear wound model resembles human wound heal process. This study was designed to prepare a hypertropic scar wound model which can be employed for testing anti-scar therapy. Four wounds were created down to the bare cartilage on the anterior side of each rabbit ear using 8-mm dermal biopsy punch and histology analysis at post operation day (POD) 5, 28 and 48 were performed. As the outcome of scar formation is largely determined by the early inflammatory response to the wounding and the degree and the duration of occlusion, cephalodin(50 mg/kg) was injected daily and medical occlusive dressings were applied. Five micro wound and scar sections were stained with hematoxylin and eosin for quantification of epidermal regeneration and scar hypertrophy. Sections were also stained using Masson's trichrome and Sirius red to evaluate collagen organization and rete ridge formation. Wound closure process was assessed to 7wks post wounding. Complete removal of the epidermis, dermis and perichondrial layer caused delayed epithelialization, which results in hypertropic scarring. The inability of the wounds to contract and the delay in epithelialization in rabbit ear was likely due to cartilage and it created scar elevation. The results suggest that full thickness surgical punch wound model in rabbit ear could be employed as a reliable and reproducible scar wound model for testing anti-scar therapy.

• 한국수산과학회지
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• 제41권5호
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• pp.330-336
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• 2008

We investigated the effect of a green seaweed Capsosiphon fulvescens extract (CFE) on the serum, liver tissue, and fecal cholesterol levels in rats. Male Sprague-Dawley rats (four weeks old) were given on of three diets for four weeks: basal, high cholesterol, and CFE, The total serum and liver tissue cholesterol levels in the CFE group were significantly decreased compared to those in the cholesterol group. The CFE group showed increased amounts of feces, total fecal bile acid and dietary fiber as compared to basal and cholesterol group. Hematoxylin and eosin staining revealed fat droplets in the livers of the rats in the cholesterol group; however, a decreased number of droplets was observed in the rats fed the CFE diet. Our results suggest that CFE supplementation may improve lipid metabolism, by controling serum and liver tissue cholesterol levels, and by increasing the total amounts of bile acid, dietary fiber and cholesterol excretion in feces.

• 대한의생명과학회지
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• 제10권3호
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• pp.285-291
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• 2004

To determine the magnetic resonance (MR) imaging findings and natural history of cerebral fat embolism in a cat model, and to correlate the MR imaging and histologic fmdings. Intemel carotid artery of 11 cats was injected with 0.1 ml of triolein. T2-weighted, T1-weighted and Gd-enhanced T1-weighted images were obtained serially for 2 hours, 1 days, 4 days, 1 week, 2 weeks and 3 weeks after embolization. Any abnormal signal intensity was evaluated. After MR imaging at 3 weeks, brain tissue was obtained for light microscopic (LM) examination using hematoxylin-eosin (HE) and Luxol fast blue staining, and for electron microscopic examination. The LM examination with HE staining revealed normal histological findings in the greater part of an embolized lesion. Cystic change was observed in the gray matter of 8 cats, while in the gray and white matter of 3 cats. At LM examination, Luxol fast blue, staining demonstrated demyelination around the cystic change occurring in the white matter, and EM examination of the embolized cortex revealed sporadic intracapillary fat vacuoles (n=11) and disruption of the blood-brain barrier (n=4). Most lesions were normal, however, and perivascular interstitial edema and cellular swelling were mild compared with the control side. The greater part of an embolized lesion showed reversible findings at MR and histological examination. Irreversible focal necrosis was, however, observed in gray and white matter at weeks 3.