• Parasites, Hosts and Diseases
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• v.49 no.2
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• pp.133-138
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• 2011

This study was undertaken to investigate the hematological and biochemical changes in experimentally infected goats with Besnoitia caprae from the time of infection till 360 days post-infection (PI). Six male goats were inoculated subcutaneously with $13{\times}10^7$ bradyzoites of B. caprae, and blood samples were collected from the jugular vein. The total erythrocyte and total leukocyte counts, hematocrit value, and differential leukocyte counts were determined. Serum biochemical analysis, including the total protein, albumin, total globulin, cholesterol, triglyceride, chloride, testosterone, calcium ($Ca^{2+}$), inorganic phosphorus, sodium ($Na^+$), potassium ($K^+$), iron ($Fe^{2+}$), glucose, serum amyloid A (SAA), haptoglobin (Hp), fibrinogen, ceruloplasmin, aspartate aminotransferase, alanine aminotransferase, creatine kinase, lactate dehydrogenase, and alkaline phosphatase, was undertaken. Skin biopsy from the limbs were collected at weekly intervals and histologically examined for Besnoitia cysts. Cysts were present in the skin biopsies of the leg of the infected goats from day 28 PI. There were variations in hematological analyses, but no significant difference was seen. From day 30 to 360 PI, results showed that SAA, Hp, fibrinogen, and ceruloplasmin concentrations increased, whereas testosterone concentrations decreased. Infected goats exhibited decrease of albumin and increase of serum total protein and globulin concentrations. By contrast, there were no significant differences in the remained analyses concentrations.

• Natural Product Sciences
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• v.17 no.4
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• pp.291-295
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• 2011

The present investigation evaluated the safety of the methanol extract from the fruit body of Phellinus pini Ames (PPA) by determining its potential toxicity after a subacute administration in rats. The extract was orally administered in doses of 1 g/kg, 2 g/kg, and 4 g/kg daily for 14 days to rats. Body weight, biochemical, and hematological parameters were determined at the end of 14 days of daily administration. The no-observed adverse effect levels (NOAEL) of the extract were 4 g/kg, when given by gavage routes. Daily oral administration of PPA extract for up to 14 days did not result in the death of significant changes in the body weight, hematological, and mainly biological parameters. In biological analysis, some significant changes occurred, including triglyceride and blood urea nitrogen (BUN), indicating that the PPA extract has liver and kidney-modulating activity. The PPA extract was found to be low or non-toxic in rats.

• Journal of Korean Medicine for Obesity Research
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• v.11 no.2
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• pp.25-32
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• 2011

Objectives: The purpose of this study was to investigate the acute toxicity with oral administration in ICR mice of Ohyaksungi-san fermented with Lactobacillus sp. Methods: In single oral administered toxic test, four groups were administrated different dosages(0, 1250, 2500, 5000 mg/kg) of fermented Ohyaksungi-san. After single oral administration, we observed number of death, clinical signs, body weight changes, hematological values and autopsy. Results: Compared with the control group, we could not find any toxic signs in the mortalities, clinical signs, body weight changes, necropsy findings and hematological values in all treated groups (1250, 2500 and 5000 mg/kg). Conclusions: These results indicate that the $LD_{50}$ value of fermented Ohyaksungi-san extract might be over 5000 mg/kg and it have no side toxic effect to ICR mice

• Journal of Veterinary Clinics
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• v.20 no.1
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• pp.115-120
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• 2003

This study was conducted to investigate the hematological changes of the dogs when administrated excessive amount of the Artemisia capillaris extract for a short period. In this experiment, clinically healthy dogs(n = 20, average weight = 7.68 kg) were divided into 2 groups : Artemisia capillaris rude juice extract group(CR) and concentrated extract group(CE). They were inserted stomach tube and administrated the extracts (5 ml/kg) for 8 days. The followings are the results of this experiment.: 1). The number of red blood cell(RBC) was significantly (p<0.05) decreased in CE group on day 4, 5, 6, whereas CJE group showed significantly (p<0.05) difference on day 5. 2). The packed cell volume(PCV) and hemoglobin concentration were significantly (p<0.05) decreased in both groups during experimental periods. 3). The met hemoglobin was significantly (p < 0.05) increased from 12 hrs after administration to day 8 in CJE group wheres, it was significantly (p<0.05) increased on day 6,7 in CE group.

• Korean Journal of Veterinary Research
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• v.30 no.1
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• pp.73-78
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• 1990

Hematological and blood chemical changes of rabbits infected with rabbit hemorrhagic disease virus were studied and the results were summarized as follows: 1. Total leukocyte count ($2,410{\pm}1,076/{\mu}l$), lymphocyte count ($1,582{\pm}632.5{\mu}l$) and heterophil count ($705{\pm}411.1{\mu}l$) were significantly decreased after 24 hours of the infection (p<0.01). However, no significant changes were observed in monocyte, eosinophil and basophil numbers. 2. A significant increase of aspartate aminotransferase (96IU/L), alanine aminotransferase (96IU/L) and alkaline phosphatase ($401.1{\pm}131.8IU/L$) was observed (p<0.01). 3. A moderate increase of BUN ($26.9{\pm}3.6mg/100ml$) and creatinine ($3.2{\pm}1.9mg/100ml$) was observed (p<0.05). 4. No significant changes of r-GTP, thymol turbidity, glucose, cholesterol, albumin, total serum protein, fibrinogen were observed.

• Journal of Veterinary Clinics
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• v.16 no.1
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• pp.57-64
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• 1999

It was performed to evaulate the effects of occlusion of mandibular gland and sublingual gland on the mandibular gland in dogs. The occlusion of mandibular gland and sublingual gland was induced by ligature in clinically normal dogs, and ultrasonograpic appearance, hematological changes and histological changes of the mandibular gland were observed daily during 6 days. The results were as follows. 1. The length, width, circumference and area of the mandibular gland in clinically normal dogs were 25.1 mm, 8.4 mm, 56.4 mm and 163.0 $\textrm{mm}^2$ in the left, and 23.4 mm, 8.0 mm, 53.3 mm and 141.6 $\textrm{mm}^2$in the right. 2. The length, width, circumference and area of the mandibular gland were gradually increased after ligature of the salivary duct, and revealed maximum values, as 33.4 mm, 10.9 mm, 73.0 mm and 287.2 $\textrm{mm}^2$, respectively, at 24 hours after ligature, and returned to normal size at 3 days after ligature. 3. The size of the opposite mandibular gland without ligature was also gradually increased until 24 hours, and returned to normal size at 3 days, similar to the changes of the mandibular gland with ligature. 4. There were no significant changes after ligature of the salivary duct in hematological findings. 5. In histopathological findings, the major of glandular alveoli was enlarged at 24 hours after ligature of the salivary duct, and neutrophils and monocytes were infiltrated at 6 days after ligature.

• The Journal of Internal Korean Medicine
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• v.15 no.2
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• pp.305-317
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• 1994

To study the effect of Kamidaebotang on the hypothyroidism induced by thiourea in SD(Splague Dawley) rats, the weight of thyroid gland, the level of serum $T_3\;and\;T_4$. total cholesterol, hematological changes and histopathological findings were measured. The following results were obtained. 1. Thyroid gland weight of the Kamidaebotang B treated group was significantly increased as compared with that of control group(p<0.05). 2. The level of serum $T_3$ of the Kamidaebotang B treated group was slightly increased as compared with that of control group. But no significant changes appeared between two groups. 3. The level of serum $T_4$ of the Kamidaebotang A, B treated groups were significantly increased as compared with that of control group(p<0.05). 4. Total cholesterol of the Kamidaebotang A, B treated groups were not significant changes as compared with that of control group. 5. Hematological data of the Kamidaebotang A, B treated groups were not significant changes as compared with that of control group. 6. Histopathologically, Kamidaebotang B administration recovered effectly the dystropy, degeneration of thyroid follicle induced by thiourea. From above results, it is suggested that Kamidaebotang B is available to the hypothyroidism induced by thiourea in rats. Therefore, Kamidabotang B seems to be applicable to hypothyroidism in clinical practice.

• 월간산업보건
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• s.346
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• pp.46-56
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• 2017

• Korean Journal of Veterinary Research
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• v.35 no.4
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• pp.843-851
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• 1995

To observe the effect of Demodex canis infection on the cellular immune response and hematological profile, 8 Doberman pinschers experimentally infected with D cains and 4 uninfected control dogs were sensitized with 2, 4-dinitro-chlorobenzene(DNCB) on the skin and were challenged with DNCB 14 days after the initial sensitization to elicit allergic contact dermatitis. Histological and hematological changes of these dogs were then observed. Macroscopic changes of skin challenged with DNCB in D canis-infected dogs included significantly reduced area of allergic reaction(p<0.05) than in uninfected control group. Infiltration of inflammatory cells in the D canis-infected group was also significantly reduced(p<0.05) than in the uninfected control group. These changes indicated that the cell-mediated immune response of the animals was suppressed by the infection with D canis. Total white blood cell count in dogs infected with D canis was increased when dogs were sensitized with DNCB (p<0.01). The result appeared to be caused by stress due to D canis infection, secondary bacterial infection and decreased efficacy of general body defense system. Blood eosionophils were increased in D canis-infected dogs which appreared to be caused by the allergic contact dermatitis. Blood chemistry analysis revealed that total protein and globulin were increased(p<0.05), while albumin level was decreased. This result appeared to be caused by secondary bacterial infection.

• Development and Reproduction
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• v.21 no.2
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• pp.181-191
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• 2017

A comparison of the growth, hematological values, fatty acids, and gonadal and growth hormonal changes of river puffer, Takifugu obscurus, tiger puffer, T. rubripes, their hybrids (river puffer ${\times}$ tiger puffer) and hybrid triploids was performed during 3 months of their early growth period. Several features were observed during these 3 months: hybrids showed the highest levels of specific growth rate, 1.48%; hybrid triploids showed the smallest change in viscera fat (P<0.05), but GSI was not significantly different among groups (P>0.05). Considering hematological parameters, hybrid triploids had increased mean corpuscular volume and mean corpuscular hemoglobin (P<0.05), but other parameters were not significantly different between groups (P>0.05). With respect to fatty acids, puffer fish, hybrids and hybrid triploids contained fatty acids such as SFAs, MUFAs, n-3 PUFAs and n-6 PUFAs. There were significantly different amounts of total fatty acids between groups (P<0.05), however, rates of changes in fatty acids did not differ significantly between groups (P>0.05). Gonadal hormone (estradiol and testosterone) changes in the river puffer and tiger puffer were significantly higher than that observed in hybrids and hybrid triploids. The hybrids and tiger puffers had higher amounts of growth hormone (thyroid stimulating hormone and thyroxine) than the hybrid triploids and river puffers (P<0.05).