• 한국식품영양학회지
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• 제30권3호
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• pp.525-530
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• 2017

King oyster mushroom (Pleurotus eryngii), an improved species of oyster mushroom, is a popular ingredient in Asian cuisine. Spleen cells were treated with various concentrations (0, 5, 10, 50, 100, 250, 500, and $1,000{\mu}g/mL$) of king oyster water extracts (KOWE); then, the proliferation of the cells was measured 24, 48, and 72 h after each treatment. Also, type 1 T helper cytokine productions ($TNF-{\alpha}$, $IFN-{\gamma}$, and IL-2) were measured in activated macrophage by KOWE in seven concentrations. Under the condition of its 50, 100, 250, and $1,000{\mu}g/mL$ for 48 h, the proliferation of cells was increased. However, there was no significant fluctuation in the spleen cells proliferation for 24 and 72 h-long KOWE exposure. To determine cytokine ($TNF-{\alpha}$, $IFN-{\gamma}$, IL-2) productions of type 1 T helper cells, macrophage was stimulated by KOWE for 48 h. Treatment of KOWE gave a rise to the levels of $TNF-{\alpha}$ and $IFN-{\gamma}$, but not in that of IL-2 productions. These results suggest that king oyster mushroom water extracts may be beneficial for enhancing immune functions in its high concentration.

• 한국경영과학회지
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• 제37권4호
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• pp.73-93
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• 2012

In recent years, service delivery systems employing a self-service approach have been rapidly spreading. Since a self-service system provides a lower product price, it attracts more customers. However, some system managers are still hesitant to accept a self-service system, because there is no systematic model to predict its performance. Therefore, this research attempts to provide a systematic and quantitative model to predict the performance of a self-service system, focused specifically on a self-service gas station. Under this model, the traditional queuing theory was adopted to describe the general self-service process, but it is also assumed that some changes occur in both the customer arrival rate and the service performance rate. In particular, the price elasticity was introduced to capture the change in the customer arrival rate, and the existence of learning effect and helpers were assumed to design the changed service performance rate. Under these assumptions, a simulation model for a self-service gas station is established, and three performance measurements, such as average number of customers, average waiting time, and Utilization are observed, depending on the changes in price difference and helper-operating time. In this research, the optimal operation strategy for price differentiation and helper-operating time is proposed in accordance with the level of the customer learning rate. Although this research confines the scope of the study to the self-service gas station model, the results of this research can be applied to any type of self-service system.

• Restorative Dentistry and Endodontics
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• 제20권1호
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• pp.235-249
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• 1995

This study was designed to identify the lymphocytes present and to examine the relation between lymphocytes population and clinical symptoms of the pulps clinically diagnosed as normal and irreversible pulpitis. We recorded the history and severity of the pain and performed several clinical tests, before extirpation of vital, irreversibly inflamed pulps in routine endodontic treatment. Then the teeth were divided into two groups. Five teeth, categorized in acute symptom group, had severe spontaneous pain, particularly at night and were extremely sensitive to cold and heat. The other 15 teeth with history of mild to moderate pain and with or without cold or heat responses were categorized as chronic symptom group. Inflamed pulps were also classified into 8 minor groups by presence or absence of signs or symptoms related to the involved teeth, including the presence of pain on percussion, pain on heat and cold stimuli and the periodontal pocket depth. All extirpated pulps were immediately immersed in ultra low-temperature freezer($-74^{\circ}C$), and they were sectioned $6{\mu}m$ in thickness. Specimens were stained using three-stage indirect immunoperoxidase techniques(DAKO, LSAB kit) and monoclonal antibodies for detecting the presence of T lymphocytes(T), B lymphocytes(B) and helper(T4) and suppressor(T8) lymphocytes. Following results were obtained; 1. All the examined normal and inflamed pull) tissues had positive staining for T lymphocytes and T helper and T suppressor cells. But B cells were observed only in inflamed pulp. 2. Statistically more T and B cells were observed in acute symptom group as compared with chronic symptom group(p<0.05). 3. Cell ratio of BIT in acute symptom group were significantly higher than that of chronic symptom group(p<0.05). 4. Only B cells were significantly increased in the percussion positive group than the number of B cells in percussion negative group(p<0.05). 5. No differences were observed in the number of different cell types among other minor groups.

• 대한물리의학회지
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• 제8권2호
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• pp.183-192
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• 2013

PURPOSE: The purpose of this study was to research the effect of self-Stretching Exercise on musculoskeletal Symptom and job Stress for care helpers who work at geriatric hospital and come up to NIOSH standard. METHODS: The effect of self-Stretching Exercise was measured using 'musculoskeletal symptom survey table' and 'Job stress measurement scale for korean' from the Korea Occupational Safety and Health Agency(KOSHA) for 40 care helpers. The paired t-test and independent t-test was used for statistical analysis. RESULTS: The pracitce of Self-Stretching Exercise was showed significant effect reducing the stress for care helper's total job Stress and inappropriate compensation, job instability, job requirement, relationship conflict, physical environment(p<.05), and significant difference for their total score, relationship conflict, structure and organization, physical environment, inappropriate compensation improvement on variations on job stress in groups(p<.05). However, self-Stretching Exercis was showed no significant effect on physcial burden. CONCLUSION: The self-Stretching Exercise was the most effect way to reduce Musculoskeletal Symptom and job Stress for a care helpers through the guidance of physical therapist.

• Archives of Pharmacal Research
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• 제21권5호
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• pp.537-542
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• 1998

to more effectively drive immune responses toward antigen-specific T helper type 2 (Th2) cell-mediated responses, we constructed a mammalian expression vetor (oPVA/IL4) carrying a fused gene in which the ovalbumin (OVA) cDNA was covalently linked to murine interleukin-4 (IL-4) cDNA. A biologically active OVA/IL4 DNA, as demonstrated by Wes tern blotting and cytokine bioassay. In tramuscular injection of BALB/c mice with the pOVA/IL4 DNA increased both the production of OVA-specific IL-4 by CD$4^{+}$ T cells and the ratio of anti-OVA lgG1 to anti-OVA lgG2a isotypes, while the injection with the pOVA DNA alone, or with the mixture of the pOVA and pIL4 DNA did no or little increase. furthermore, the OVA-specific, Th2 cell-mediated immune responses were significantly enhanced by multiple injections with the pOVA/IL4 DNA. These studies indicate that the direct linkage of an OVA gene to an IL-4 gene in the expression plasmid confines the effects of IL-4 to the OVA-specific cells, efficiently driving the immune response toward OVA-specific, Th2 cell-mediated responses.

• Food Science and Biotechnology
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• 제18권6호
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• pp.1316-1321
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• 2009

Multiple beneficial properties of Opuntia ficus indica (OPF) are well established. In the present study, we have investigated the immunological role of OPF extract (OPFE) on murine splenocytes. OPFE dose- and time-dependently enhanced the proliferation of splenocytes without cytotoxicity. Our results also showed that the number of $CD4^+$ helper T cells and CD45R/$B220^+$ pan B cells increased markedly, but not $CD8^+$ cytotoxic T cells or $CD11b^+$ granulocytes/macrophages. In addition, OPFE significantly decreased the production levels of T helper (Th) 1 type cytokines, interferon (IFN)-$\gamma$, and tumor necrosis factor (TNF)-$\alpha$, although had no significantly differences in those of interleukin (IL)-4, a Th2 type cytokine in concanavalin A (Con A)-stimulated blastogenic cells. Furthermore, OPFE alone strongly increased IL-4 production and decreased TNF-$\alpha$ production even in the absence of Con A. On the basis of these results, this study suggests that OPFE enhances immunity by regulating the pro- and anti-inflammatory response, indicating that this extract exerts a marked immunomodulatory effect, confirming its usefulness as therapy for immune-related diseases.

• 가정과삶의질연구
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• 제32권2호
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• pp.27-40
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• 2014

The purpose of this study is to examine the motive of participating in the care work, the overall cognition survey for the care work and the infant care practice areas required in the care work for the child care helpers who activate in the care work, and search for methods of specializing infant care and care experiences on the results. Results of study showed that firstly, middle- or the late middle-age women participated in the care worker recognized care work as productive labor to create economic value and that secondly, the child care helpers' recognition degree of self-development and job satisfaction through performance of care work was wholly high level and that there was will of participation in training for self-development but actual participation rate was low due to restrictions by general conditions. Thirdly, the infant care practice areas and their particular contents that the child care helpers recognized in the care work necessary were highly shown in the whole. The requested level of education for safety management, play guidance, humanity guidance for infant and child was high. Point to discuss is that occupation stability and occupation image must be raised through the public job-hunting system for the child care helpers. In addition, a tailor-made education support suitable for the child care helpers' career stage is needed. Education support by the performance of infant care practice area and the educational requirements must be provided to major rearers to ensure that they can function as behavioral and emotional supporters. Finally, a support system for continuous self-management.

• BMB Reports
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• 제47권7호
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• pp.399-404
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• 2014

B7-H4 is a member of B7 family of co-inhibitory molecules and B7-H4 protein is found to be overexpressed in many human cancers and which is usually associated with poor survival. In this study, we developed a therapeutic vaccine made from a fusion protein composed of a tetanus toxoid (TT) T-helper cell epitope and human B7-H4IgV domain (TT-rhB7-H4IgV). We investigated the anti-tumor effect of the TT-rhB7-H4IgV vaccine in BALB/c mice and SP2/0 myeloma growth was significantly suppressed in mice. The TT-rhB7-H4IgV vaccine induced high-titer specific antibodies in mice. Further, the antibodies induced by TT-rhB7-H4IgV vaccine were capable of depleting SP2/0 cells through complement-dependent cytotoxicity (CDC) in vitro. On the other hand, the poor cellular immune response was irrelevant to the therapeutic efficacy. These results indicate that the recombinant TT-rhB7-H4IgV vaccine might be a useful candidate of immunotherapy for the treatment of some tumors associated with abnormal expression of B7-H4.

• IMMUNE NETWORK
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• 제13권2호
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• pp.63-69
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• 2013

IL-12 is a secretory heterodimeric cytokine composed of p35 and p40 subunits. IL-12 p35 and p40 subunits are sometimes produced as monomers or homodimers. IL-12 is also produced as a membrane-bound form in some cases. In this study, we hypothesized that the membrane-bound form of IL-12 subunits may function as a costimulatory signal for selective activation of TAA-specific CTL through direct priming without involving antigen presenting cells and helper T cells. MethA fibrosarcoma cells were transfected with expression vectors of membrane-bound form of IL-12p35 (mbIL-12p35) or IL-12p40 subunit (mbIL-12p40) and were selected under G418-containing medium. The tumor cell clones were analyzed for the expression of mbIL-12p35 or p40 subunit and for their stimulatory effects on macrophages. The responsible T-cell subpopulation for antitumor activity of mbIL-12p35 expressing tumor clone was also analyzed in T cell subset-depleted mice. Expression of transfected membranebound form of IL-12 subunits was stable during more than 3 months of in vitro culture, and the chimeric molecules were not released into culture supernatants. Neither the mbIL-12p35-expressing tumor clones nor mbIL-12p40-expressing tumor clones activated macrophages to secrete TNF-${\alpha}$. Growth of mbIL-12p35-expressing tumor clones was more accelerated in the $CD8^+$ T cell-depleted mice than in $CD4^+$ T cell-depleted or normal mice. These results suggest that $CD8^+$ T cells could be responsible for the rejection of mbIL-12p35-expressing tumor clone, which may bypass activation of antigen presenting cells and $CD4^+$ helper T cells.

• 한국통신학회논문지
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• 제39A권11호
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• pp.682-689
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• 2014

모바일 데이터 트래픽(data traffic)의 증가로 인해 스몰셀(Small Cell) 가용을 이용한 여러 기술들이 해결책으로 제시되고 있다. 하지만 이러한 기술은 백홀(backhaul) 용량의 증가가 필수적이고 이가 충족되지 않을 경우 성능한계가 기인한다. 이를 바탕으로 기지국 내 헬퍼(helper)라는 백홀 용량이 없거나 작은 대신 저장 매체 용량을 지닌 스몰셀을 이용해 백홀 용량을 저장 매체 용량으로 대체하여 사용자의 데이터 다운로드 지연값을 줄이는 펨토-캐싱(Femto-Caching)이 제시되었다. 본 논문은 사용자 위치 분포를 이용하는 실제적인 시나리오에서 각 헬퍼에 파일 분배 전략 방법을 제시함으로써 기존 연구를 확장하였다. 시뮬레이션을 통해 헬퍼간 커버리지(Coverage)가 겹치는 상황과 헬퍼의 커버리지가 증가하는 상황에 대해 사용자의 데이터를 헬퍼로부터 받을 수 있는 기회가 증가함을 통해 성능을 검증하였다.