• 방송공학회논문지
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• 제23권1호
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• pp.53-62
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• 2018

스토리를 분석하기 위해서는 스토리의 행동 주체인 등장인물에 대한 분석이 선행되어야 한다. 현재의 등장인물 분석 방법인 Character-net이나 RoleNet과 같은 방법은 최종 누적된 스토리의 결과에 대한 분석만을 진행하여 조연의 역할 분류를 하기에는 부족한 면이 있다. 조연의 역할 분석을 위해 축적된 형태의 스토리 분석 방법이 아닌 스토리 진행에 따른 시계열적 분석 방법을 연구할 필요가 있다. 본 논문은 Character-net의 3차원 시각화를 통해 등장인물들 중에 조력자들을 유형에 따라 멘토와 단짝친구로 분류하는 방법을 제안하고 그 방법의 정확도를 평가하는 논문이다. 3차원 시각화를 위해 WebGL을 사용하여 웹브라우저 상에서 누구나 결과를 확인할 수 있도록 인터페이스를 구성하였다. 또한 멘토와 단짝친구를 구별하기 위한 규칙을 제안하였고 그 성능을 평가하였다. 영화 7편에 대해 선정된 10명의 등장인물에 대해 평가한 결과 90%의 정확도를 나타내는 것을 확인하였다.

• Toxicological Research
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• 제34권1호
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• pp.7-12
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• 2018

Laboratory animal models have been developed to investigate preventive or therapeutic effect of medicinal products, or occurrence or progression mechanism of atopic dermatitis (AD), a pruritic and persistent inflammatory skin disease. The murine model with immunologic phenomena resembling human AD was introduced, which demonstrated skewedness toward predominance of type-2 helper T cell reactivity and pathophysiological changes similar as human AD following 2,4-dinitrochlorobenzene (DNCB) sensitization and challenge. Molecular mechanism on the DNCB-mediated AD was further evaluated. Skin tissues were collected from mice treated with DNCB, and each tissue was equally divided into two sections; one for protein and the other for mRNA analysis. Expression of filaggrin, an important protein for keratinocyte integrity, was evaluated through SDS-PAGE. Level of mRNA expression for cytokines was determined through semi-quantitative reverse transcriptase polymerase chain reaction. Expression of filaggrin protein was significantly enhanced in the mice treated with DNCB compared with the vehicle (acetone : olive oil = 4 : 1 mixture) treatment group or the normal group without any treatment. Level of tumor necrosis factor-alpha and interleukin-18 mRNA expression, cytokines involved in activity of type-1 helper T ($T_H1$) cell, was significantly downregulated in the AD group compared with other control groups. These results suggest that suppression of $T_H1$ cell-mediated immune response could be reflected into the skin tissue of mice treated with DNCB for AD induction, and disturbance of keratinocyte integrity might evoke a compensatory mechanism.

• 동의생리병리학회지
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• 제18권5호
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• pp.1347-1355
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• 2004

In this study, the immunological effect of a traditional Korea herbal acupuncture, that has been widely used for the treatment of various immunological disorders including inflammation in Korea, was examined in vitro and in vivo. In our previous study demonstrated that BV increased the expression of IFN-γ mRNA, that plays pivotal role in T cell response. This study was designated to evaluate the effect of BV on helper T cell development by monitoring Th1/Th2 specific cytokine secretion patterns in artificially induced Th1/Th2 polarized condition and in vivo. The results demonstrated that BV didn't have mitogenic effects on the unstimulated CD4+ T cell, but increased the CD4+ T cell proliferation upon activation with anti-CD3/CD28 antibody. The Th1 cells were over-populated dramatically in Th1 driven condition with BV treatment, while the Th2 cells were increased slightly in Th2 skewed condition. Furthermore, under Th1-skewed conditions, the level of IFN-γ was considerably increased with BV treatment. Besides, the expression of T-bet, a transcription factor that plays pivotal role in Th1 lineage programming, was increased with BV treatment. The expressions of IFN-γ and T-bet were also significantly increased in vivo. The results that Th1 specific cytokine secretion were considerably increased and Th2 specific cytokine secretion were not significantly changed in vitro and in vivo indicated that BV enhances Th1 lineage development, Therefore, these results suggest that BV might be desirable agent for correction of Th1 dominant pathological disorders.

• 동의생리병리학회지
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• 제18권5호
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• pp.1397-1403
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• 2004

By recently study, GM (Gamipaemo-tang) treatment have worked well on the allergic asthma. The purpose of this study was effect of GM extract on helper T cell, major regulator of immune system. Splenic cells from 8-week BALB/c mice were cultured in GM containing media without activation for 48 hours. The MTS assay and flow cytometry study revealed that lymphocyte treated with GM were not effective on CD4+ T cells. Subsequently CD4+ T cells were isolated and cultured in GM containing media. Either GM were not effective on CD4+ T cell without APCs. By FACS scan analysis, the expression of INF-γ, IL-4 were down-regulated in the condition skewed Th1 and Th2 cells respectively, Using ELISA analysis, the expression of INF-γ is up-regulated and IL-4 is down-regulated in the condition skewed Th1, Th2 cells respectively. With RT-PCR analysis, the expression of mRNA for INF-γ is down-regulated and IL-4 is down-regulated in the condition skewed Th1 and Th2 cells respectively. The result suggests that GM inhibited the differetiation of Th2 cells significantly and indicates GM could enhance anti-allergic immune system.

• The Plant Pathology Journal
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• 제27권4호
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• pp.315-323
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• 2011

Soybean plants infected with Bean pod mottle virus (BPMV) develop acute symptoms that usually decrease in severity over time. In other plant-virus interactions, this type of symptom recovery has been associated with degradation of viral RNAs by RNA silencing, which is accompanied by the accumulation of virus-derived small interfering RNAs (siRNAs). In this study, changes in the accumulation of BPMV siRNAs were investigated in soybean plants infected with BPMV alone, or infected with both BPMV and Soybean mosaic virus (SMV) and in transgenic soybean plants expressing SMV helper component-protease (HC-Pro). In many potyviruses, HC-Pro is a potent suppressor of RNA silencing. In plants infected with BPMV alone, accumulation of siRNAs was positively correlated with symptom severity and accumulation of BPMV genomic RNAs. Plants infected with both BPMV and SMV and BPMV-infected transgenic soybean plants expressing SMV HC-Pro exhibited severe symptoms characteristic of BPMVSMV synergism, and showed enhanced accumulation of BPMV RNAs and siRNAs compared to plants infected with BPMV alone and nontransgenic plants. Likewise, SMV HC-Pro enhanced the accumulation of siRNAs produced from a silenced green fluorescent protein gene in transient expression assays, while the P19 silencing suppressor of Tomato bushy stunt virus did not. Consistent with the modes of action of HC-Pro in other systems, which have shown that HC-Pro suppresses RNA silencing by preventing the unwinding of duplex siRNAs and inhibiting siRNA methylation, these studies showed that SMV HC-Pro interfered with the activities of RNA-induced silencing complexes, but not the activities of Dicer-like enzymes in antiviral defenses.

• 정보보호학회논문지
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• 제26권1호
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• pp.69-79
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• 2016

생체 정보를 이용한 사용자 인증은 사용자가 어떠한 정보도 소유하거나 기억할 필요가 없으므로 사용자 측면에서 매우 편리하다. 그러나 생체 정보는 한번 노출되면 영구적으로 사용할 수 없기 때문에 인증 수행 시 생체 정보의 프라이버시 보호가 필수적이다. 또한 생체 정보는 본질적으로 노이즈가 있기 때문에 인증 수행 시 적절한 오차 범위 이내에서 이를 처리할 수 있어야 한다. 최근 퍼지 추출기(fuzzy extractor)를 이용한 생체 정보 기반 인증 프로토콜에 대한 연구가 활발히 진행되고 있으나, 사용자가 헬퍼 데이터(helper data)를 추가적으로 기억해야 하는 문제점이 존재한다. 본 논문에서는 함수 암호 중 하나인 HV-KEM(Hidden Vector Key Encapsulation Mechanism)을 이용하여 사용자가 어떠한 정보도 소유하거나 기억할 필요 없는 생체 정보 기반 인증 프로토콜을 제안한다. 또한, 인증 프로토콜 설계를 위한 HV-KEM의 보안 요구사항을 정립하고 제안하는 인증 프로토콜을 정확성/안전성/효율성 측면에서 분석한다.

• 대한한방소아과학회지
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• 제18권1호
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• pp.221-246
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• 2004

Objective: The purpose of this study was to investigate the effects of Kamikwiryongtang (KKT) on the immune response and growth in a young mouse (3 weeks mice). Methods The viability of thymocytes and splenocytes in vivo and in vitro system, the population of helper T (Th) cells and cytotoxic T (Tc) cells in thymocytes and increased the population of T-lymphocytes and the population of Th cells in splenocytes, the production of ${\gamma}$ -interferon, interleukin-2 and interleukin-4 in splenocytes was investigated. KKT (500mg/kg) was administerd p.o. once a day for 7 days. Results: KKT increased the viability of thymocytes and splenocytes in vivo, but did not affect the viability of thymocytes and enhanced the viability of splenocytes in vitro system. In addition, KKT did not affect the population of helper T (Th) cells and cytotoxic T (Tc) cells in thymocytes and increased the population of T -lymphocytes and the population of Th cells in splenocytes. Also, KKT increased the production of ${\gamma}$-interferon, interleukin-2 and interleukin-4 in splenocytes. Furthermore, KKT increased the production of nitric oxide in vivo, but did not affect the production of nitric oxide in vitro system. KKT enhanced the phagocytic activity of peritoneal macrophages in vivo, but decreased the phagocytic activity in vitro system: KKT increased the body weight of a young mouse. Conclusions: KKT stimulates the specific immune response via increase of, the viability of thymocytes and splenocytes and the non-specific immune response via increase of phagocytic activity of peritoneal macrophages and stimulates the growth of a young mouse.

• 동의생리병리학회지
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• 제18권2호
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• pp.580-585
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• 2004

BJYGC is often clinically used as a treatment of allergic rhinitis. This study was aimed to find out the effect BJYGC would have on the helper T cell, and how it can promote the subsets of helper T cells to regain their balance that they lost due to immunological diseases. Splenocytes were prepared from BALB/c mice was cultured without stimulation in the presence of BJYGC for 48 hr. The viability of CD4 T cells from Balb/c mouse were measured at various concentrations of BJYGC using the MTS assay. It was somewhat increased up to concentration of 400 ㎍/ml, but did not show any significant difference. Proliferation was measured using the MTS assay, CD4 Th cells were stimulated with anti-CD3/28 in the presence of BJYGC for 48 hr. As evidence for rapid T cell activation, CD25 expression by flow cytometry was evaluated at 10, 50, 100 and 200 ㎍/㎖ of BJYGC. Th cell differentiation experiments were performed to examine whether BJYGC can affect the Th polarization process. CD4 T cells were activated in culture under neutral, Th1-polarized or Th2-polarized conditions in the presence of BJYGC at 10, 100 and 200 ㎍/㎖. Cytokine production was measured by ELISA. This experiment proved that BJYGC could inhibit the secretion of both IL-4 and IFN-γ in neutral condition and polarized condition, too. Considering that BJYGC shows an excellent effect on treating allergies, the author can conclude that its pharmacological action may be associated with decreased IL-4 and, it may also regulate IFN-γ depending the host's need. Also, it was discovered that Th1 cell was pathologic in chronic inflammatory tissue specific diseases, such as insulin dependent diabetes mellitus, multiple sclerosis, RA, and uveitis. We are counting on the BJYGC to be able to control the tendency of Th1 cell predominancy in an immune reaction.

• 한국콘텐츠학회논문지
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• 제20권5호
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• pp.500-512
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• 2020

본 연구는 리조트 조직의 저변에 잠재해 있으며 리조트 직원들에 의해 공유되어야 하는 조직문화를 발견하는데 목적이 있다. 최근 힐링에 대한 욕구와 지역사회의 라이프스타일 체험을 강조하는 추세에 주목해 볼 때 이는 리조트가 원래 탄생 시기부터 가지고 있었던 고유의 역할이었다. 그럼에도 불구하고 최근 리조트가 호텔 산업과 경계가 모호해지고 테마파크를 닮은 복합리조트로 변화하는 등 정체성이 흔들리고 있음을 부인할 수 없다. 리조트의 조직문화를 찾아내기 위해 16명의 리조트 전문가들을 대상으로 그레마스 행위소 모형을 기초로 제작된 심층인터뷰를 진행하였는데 3박4일간의 리조트 체험을 통해서 응답내용의 충실성을 유도하였다. 모형이 가지고 있는 6개의 행위소를 리조트를 구성하는 제반 요소와 합치시키고 응답자로 하여금 자유롭게 은유적으로 표현하도록 하였다. 응답자들은 발신자, 수신자, 주체, 객체, 보조자, 반대자를 각각 리조트, 리조트 방문객, 리조트기업, 리조트상품서비스, 리조트지원집단, 리조트방해집단에 대응시킨 결과 순서대로 심신치유, 환자, 치유사, 치유약, 치유도우미, 돌팔이약장수로 은유하였다. 은유한 내용은 모형에 따라 신화(이야기)로 구성하였으며 더 나가 조직문화에 주는 시사점으로 연결하였다. 연구의 이론적 실무적 시사점 및 한계점은 논문 말미에 포함시켰다.

• 한방안이비인후피부과학회지
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• 제24권1호
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• pp.86-95
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• 2011

Objectives : Ulmus davidiana (UD) has been widely used in Korean herbal medicines used for treatment of acute and chronic inflammatory diseases, such as rhinitis, asthma, and abscess. In this study, To investigated the protective effect of UD on type 1 allergic response, we determined whether UD inhibits early and late allergic response. Methods : The effect of UD was analyzed by ELISA and RT-PCR in RBL-2H3 cells. Levels of ${\beta}$ -hexosaminidase, interleukin (IL)-4 and TNF-${\alpha}$ were measured using enzyme-linked immunosorbent assays (ELISAs). mRNA levels of COX-2 and T-helper type 2(Th2) cytokines were analyzed with RT-PCR. Results : We found that UD suppressed ${\beta}$-hexosaminidase release in RBL-2H3 not only by the PMA plus A23187 stimulation, but also by the IgE-DNP-HSA stimulation at the antigen-antibody binding stage and antibody-receptor binding stage. UD also significantly inhibited COX2 level, along with reduced Th2 cytokine levels, such as IL-3, IL-4, IL-5, IL-13, GM-CSF, and TNF-${\alpha}$ in RBL-2H3. Conclusions : Our results indicate that UD protects against type 1 allergic response and exerts an anti-inflammatory effect through the inhibition of degranulation and expression of COX2 and Th2 cytokines.