• Journal of Korean Society of Forest Science
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• v.84 no.4
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• pp.424-431
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• 1995

This study was conducted to evaluate resistance and physiological responses of Zelkova serrata Makino seedlings to ozone in open-top chamber. One-year-old seedlings of Zelkova serrata were planted in pots in April and grown in greenhouse until August. The plants were transferred into two out-door open-top chambers with a dimension of 2.0 m in diameter and 2.0 m in height. First chamber served as a control and was supplied with ambient air. Ozone was added to the second chamber for 5 hours per day(10.00 AM-15.00 PM) for 23 consecutive days at 0.1 ppm. Each chamber housed 70 pots. Every two, three or five days after initiation of exposure, ten pots were randomly removed from the chamber and determined for the contents of chlorophyll a, b, total chlorophyll and ${\beta}$-carotene in the leaves. Photosynthesis and dark respiration were estimated by measuring $CO_2$ absorption in a gas exchange chamber and oxygen absorption by oxygen monitoring system, respectively. Superoxide dismutase(SOD) activity in the leaves was assayed by a xanthine oxidase method. First visible injury of translucent(water-soaked looking) spots appeared on the leaves 14 days after the initial exposure, and ozone accelerated senescence of old leaves. Contents of chlorophyll a and b decreased by 17%, and 31%, respectively, in ozone treatment two days after exposure. The decrease in chlorophyll b was greater than that of chlorophyll a. Content of ${\beta}$-carotene in ozone treatment decreased by 25% two days after initiation of exposure, but the reduction was recovered with time. Photosynthesis decreased by 45%, and the respiration increased by 28% in the ozone treatment. SOD activity started to increase 4 days after beginning of exposure and increased by 285% 7 days after exposure, and decreased to the level below the control treatment with the advancement of the visible injury.

• Journal of Bio-Environment Control
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• v.4 no.2
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• pp.152-158
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• 1995

This experiment was undertaken in order to clarify effect of NO$_3$-N/NH$_4$-N ratios(NO$_3$/NH$_4$ : 10:0, 8:2) in the nutrient solution on growth, yield, photosynthetic rate, relative concentration of chlorophyll and root activity of hydroponically grown pepper plants at three different root- zone temperatures of 18$^{\circ}C$, 22$^{\circ}C$ and 26$^{\circ}C$. Plant height, leaf number, stem diameter, fresh and dry weight of leaf and root were no effect in by three root- zone temperatures. However, leaf number, stem diameter, fresh and dry weight of leaf and stem, dry weight of root at 18$^{\circ}C$, 22$^{\circ}C$ and $25^{\circ}C$ increased when NH$_4$-N was added to the solution. Under root-Bone temperatures of 18$^{\circ}C$, 26$^{\circ}C$ condition, fruit length were longer by the addition of NH$_4$-N. Fruit number and yield increased by the addition of NH$_4$-N at three root-zone temperatures. Photosynthetic rate decreased as root - zone temperature increased. Under root-zone temperatures of 18$^{\circ}C$, 22$^{\circ}C$ and 26$^{\circ}C$ condition, photosynthetic rate increased significantly by the addition of NH$_4$-N. Chlorophyll content of plants increased at 22$^{\circ}C$. Under root-zone temperatures of 18$^{\circ}C$, 22$^{\circ}C$ and 26$^{\circ}C$ condition, chlorophyll content of plants increased by the addition of NH$_4$-N. Root activity of increased at 26$^{\circ}C$ Under root-Bone temperatures of 18$^{\circ}C$, 22$^{\circ}C$ and 26$^{\circ}C$ condition, root activity increased by the addition of NH$_4$- N.

• Journal of Bio-Environment Control
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• v.25 no.2
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• pp.83-88
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• 2016

In this study, previously reported surplus solar energy-related study result and current status of fan coil unit (FCU) for cooling and heating installed in the current sites were briefly examined and then a method to determine the number of FCUs required to recover surplus solar energy was schematically proposed to provide basic data for researchers and technical engineers in this field. The maximum, mean, and minimum outside temperatures during the experiment period were about $28.2^{\circ}C$, $4.4^{\circ}C$, and $-11.5^{\circ}C$, respectively. The horizontal surface solar radiation level outside the greenhouse was in a range of $0.8-20.5MJ{\cdot}m^{-2}$ and mean and total solar radiation were $10.8MJ{\cdot}m^{-2}$ and $1,187.5MJ{\cdot}m^{-2}$. The mean temperature and relative humidity in the greenhouse during the daytime were in a range of 18.8-45.5 and 53.5-77.5%. The total surplus solar energy recovered from the greenhouse during the experiment period was approximately 6,613.4MJ, which could supplement about 6.7% of the total heating energy 98,600.2 MJ. In addition, the number of FCUs installed for heating varies case to case, although similar FCUs are used. Thus, it is necessary to study the installation height, orientation and installation distance as well as the appropriate number of FCUs from the efficient and economical viewpoints. The required numbers of FCUs for surplus solar energy recovery were 8.4-10.9units and 6.1-8.0units based on air mass and circular flow rate that passed through the FCUs. Considering calculation methods and the risks such as efficiency and use environments of FCUs, it was found that about nine units (one unit per $24m^3$ approximately) needed to be installed. The required number of FCUs for surplus solar energy recovery was around one unit per $24m^3$ approximately.

• Journal of Bio-Environment Control
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• v.17 no.3
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• pp.221-225
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• 2008

Lilium formolongi 'Fl August' plantlets with scale-leaves and scale-bulb were treated at 10, 15, 20, $25^{\circ}C$ for 15, 30, or 45 days and planted in February, March, April and May. In the April planting, flowering percentage was below 10% and in the May planting no flowering occurred. Sprouting and flowering percentages were lower at the late planting times. Days to flowering of the April planting was 110.8 days compared to 128 days for the February planting. Plant height and numbers of leaves were reduced to 7.2 in May planting, compared to 40.5 leaves in February planting, and almost no flowers emerged in either the April or May plantings. Plantlets exposed to 10 or $15^{\circ}C$ flowered at 80 percent or higher at all treatment durations, but at 20 or $25^{\circ}C$ flowering percentages were lower, with 30% or less in the $25^{\circ}C$ treatment. In the $15^{\circ}C$ treatment days to flowering were less than 100 days, while the number of flowers and flower bud differentiation were greatest in the $15^{\circ}C$ treatment. Cytokinin and auxin were analyzed in bulblets grown at 15, 20 and $25^{\circ}C$. T-zeatin content was three times greater in the $15^{\circ}C$ treatment than at $25^{\circ}C$, but the content of indoleacetic acid (IAA) was less at $15^{\circ}C$ than at 20 and $25^{\circ}C$. In the $15^{\circ}C$ treatment, T-zeatin content was about twice the IAA content in the scale- bulblet. The auxin and cytokinin balance may affect flower bud differentiation. $15^{\circ}C$ with 30 days was most effective for flowering in scale-bulblet and planting of February and March were effective for flowering too.

• Korean Journal of Soil Science and Fertilizer
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• v.18 no.2
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• pp.148-155
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• 1985

This study was conducted to find out the effect of rice straw and wollastonite applied to the paddy soil on the growth and yield of the rice plant. The soil was treated with two level of straw 0 and 88gr per pot, of which respectively combined with 4 levels of wollastonite, 0 (Avail $SiO_2$: 50 ppm), 27 (Avail. $SiO_2$: 150 ppm), 85 (Avail. $SiO_2$: 300 ppm) and 185 (Avail. $SiO_2$: 450 ppm) gr per pot. The results for two years experiment were as follows. 1. Application of rice straw and wollastonite, respectively, suppressed the growth of the plant at early stage. As a consequence the hight and number of tiller of the plant were comparatively smaller, but the combined effect of rice straw and wollastonite treatment on the plant growth appeared after the maximum tillering stage and got a better yield as compared with single treatment of rice straw or wollastonite. 2. At the pot of no rice straw and 27 gr wollastonite treatment, and also pot of rice straw and 85gr (in the year of 1983 experiment) as well as 135gr wollastonite treatment (in the year 1984 experiment), the yield of total dry material and unhulled rice of the plant were respectively higher than other pots in different treatment and the plant height showed similar tentency. Application of rice straw in paddy soil appeared to require more wollastonite for growth of the rice plant as compared with no rice straw application. 3. The highest yield was obtained at the pot with rice straw and 85gr of wollastonite treatment in the year of 1983 experiment, were its yield had 25% increament as compared with the control pot, while being studied for residual effect of rice straw and wollastonite application in the next year, the highest yield was obtained at the pot with rice straw and 135gr wollastonite treatment, where its yield had 30% increament as compared with the control pot.

• Korean Journal of Poultry Science
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• v.43 no.1
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• pp.15-20
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• 2016

This research was carried out to determine the effect of Cudrania tricuspidata fruit (CTF) on the performance of laying hens, egg quality, yolk fatty acid composition, Immunoglobulin G (IgG) and blood composition. A total 288 Brown Nick laying hens of 48 weeks old replaced into 4 groups with 4 replicates of 18 birds in each. Treatments were added with 0, 0.25, 0.5, 1.0% CTF containing dried seed and pulp. Metabolizable energy (ME) and crude protein (CP) were 2,800 kcal/kg and 16.0% in basal diets, respectively. Egg production and daily eggmass were significantly increased in 1.0% CTF addition treatment compared to those of other treatments (P<0.05). Egg weight and feed intake were not different among treatments. Feed conversion ratio showed the lower result in 1.0% CTF treatment than other treatments (P<0.05). Eggshell color was not influenced by the addition of CTF. Albumin height, Haugh unit and eggshell breaking strength was not statistically different, but seemed to be higher as dietary CTF addition increased. Yolk color was increased as dietary CTF increased and was the highest in 1.0% CTF group (P<0.05). Fatty acids were not shown the consistency in yolk. Blood cholesterol and triglyceride (TG) of birds fed CTF were significantly low compared to those of control. IgG was also statistically higher in CTF treatments than control (P<0.05). As the results of this experiment, the addition of 1% CTF would be the most effective on performance and egg quality, whereas the 0.25% CTF addition in basal diets seemed to be optimum to improve the blood cholesterol, TG,high density lipoprotein cholesterol (HDL-CHOL), IgG in laying hens.

• The Journal of Korean Academy of Prosthodontics
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• v.49 no.3
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• pp.245-253
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• 2011

Purpose: The aim of this in vitro study was to estimate surface characteristic after peptide coating and investigate biological response of human mesenchymal stem cell to anodized titanium discs coated with RGD peptide by physical adhesion and chemical fixation. Materials and methods: Fluorescence isothiocyanate (FITC) modified RGD-peptide was coated on the anodized titanium discs (diameter 12 mm, height 3 mm) using two methods. One was physical adhesion method and the other was chemical fixation method. Physical adhesion was performed by dip and dry procedure, chemical fixation was performed by covalent bond via silanization. In this study, human mesenchymal stem cell was used for experiments. The experiments consisted of surface characteristic evaluation after peptide coating, analysis about cell adhesion, proliferation, differentiation, and mineralization. Obtained data are statistically treated using Kruskal-Wallis test and Bonferroni test was performed as post hoc test (P=.05). Results: The evaluation of FE-SEM images revealed no diffenrence at micro-surfaces between each groups. Total coating dose was higher at physical adhesion experimental group than at chemical fixation experimental group. In cell adhesion and proliferation, RGD peptide coating did not show a statistical significance compared with control group (P>.05). In cell differentiation and mineralization, physical adhesion method displayed significantly increased levels compared with control group and chemical fixation method (P<.05). Conclusion: RGD peptide coating seems to enhance osseointegration by effects on the response of human mesenchymal stem cell. Especially physical adhesion method showed more effective than chemical fixation method on response of human mesenchymal stem cell.

• Clinical and Experimental Pediatrics
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• v.48 no.1
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• pp.34-39
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• 2005

Purpose : Perinatal complications associated with maternal smoking and alcohol ingestion in pregnancy have been well documented. But until now there has been no clinical data on it collected in our country. In this paper, we tried to research the association between maternal smoking and alcohol ingestion in pregnancy and the physical indices of the neonate at delivery among unmaried mothers from one charitable institution. Methods : We enlisted 125 unmarried with a history of smoking and alcohol consumption during pregnancy who delivered a baby in National Medical Center from March, 2001 to March, 2004 as a study group. As a control, 174 unmarried mothers without a history of smoking and alcohol were enlisted. Then, we compared the physical indices - birth weight, height, and the head circumference - of the neonates from both groups. Furthermore, we investigated the effects of the period and the quantity of maternal smoking and alcohol ingestion on the physical indices of the neonates from the both groups. Results : Low physical indices of neonates were found in the study group(either smoking group, or drinking group) compared with the control group. The quantity of smoking or drinking and the period of smoking or drinking have no significant effect on physical indices. Conclusion : We found that maternal smoking and alcohol result in the low physical index of neonates, and educational initiatives must be directed at expectant mothers to emphasize the harmful effects of smoking and alcohol ingestion in pregnancy.

• Journal of Bio-Environment Control
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• v.13 no.4
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• pp.233-239
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• 2004

This research was conducted to determine the effect of soil pH change followed by leaching with ground water on crop growth, yield and nutrient uptake of strawberry. Treatments were leaching with ground water (A), hydrated lime application of dissolved portion in $500g{\cdot}L^{-1}$ solution followed by leaching (B), application of $Al_2SO_4$ solution with the concentration of $0.75g{\cdot}L^{-1}$ followed by leaching (C), pH elevation by (B) followed by pH lowering by (C), then leaching (D). Soil samples taken before experiment had 6.0 in soil pH. But the pH of all treatments taken after pH changes and leaching were in the range from 5.8 to 6.2 without statistical differences among treatments. Electrical conductivity of soils taken before experiment was $1.82dS{\cdot}m^{-1}$ and those of (A), (B), (C), and (D) were 1.65, 1.72, 1.71 and $1.83dS{\cdot}m^{-1}$, respectively. The plant height in treatment (C) was 20.4 cm at 80 days after transplanting which was the highest among treatments tested. The other growth characteristics did not show statistical differences among treatments. Tissue contents of Fe, Mn, Zn, Cu and B in treatment (C) were higher than those of other treatments at 80 days after transplanting. The treatment (C) had the highest number of special sizes and fruit weight per 50 plants such as 137 and 3.784 g, respectively. The treatment also had 3.123 kg in total fruit weight and 2,489 kg in marketable fruit weight per 10 are. The treatment (B) also had good results such as 2,820 kg in marketable fruit weight per 10 are.

• Journal of Bio-Environment Control
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• v.3 no.2
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• pp.119-127
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• 1994

This study was conducted to investigate the effects of temperature and light conditions on the vigor and growth responses of red pepper(Capsicum annuum L.) seedlings in the nonheated plastic houses with triple and quadruple coverings during winter. The results obtained were as follows ; 1. The growth in terms of plant height, number of leaves, leaf area and dry weight of each organ was reduced up to 50% in the triple coverings compared to quadruple ones. The greatest difference between the triple coverings and quadruple ones was the leaf area, showing two to five times larger in the quadruple ones. Therefore, the differences of the early environmental conditions during raising seedling stage was well reflected. 2. The leaf expansion in the early stage of seedling was delayed in the triple coverings and subsequently crop growth rate(CGR) significantly reduced. The specific leaf area (SLA) in the triple coverings was less than quadruple ones. The net assimilation rate (NAR) was increased in the early stage of seedlings and then reduced in the quadruple coverings. In triple ones, however, the great reduction showed at six weeks after sowing and then increased sharply. 3. The linear relationship among leaf area, total dry weight and leaf dry weight was observed in the all experimental areas, while between leaf area and crop growth rate only in the quadruple coverings, and between leaf area and net assimilation rate in both triple and quadruple ones. 4. The dry matter partitioned to the leaf increased up to six weeks after sowing in the triple coverings but reduced in the stem and root. However, the adverse results were obtained in the quadruple ones. 5. In the triple coverings, the number of branches showed the exponential increment while fruit weight showed linear increment in both triple and quadruple ones. Higher yield was obtained in the quadruple ones. 6. The shoot dry weight among the characteristics of seedlings was greatly contributed to the yield of red pepper after transplanting. Accordingly, one of the decisive criterion for good quality of red pepper seedlings was determined by the highest correlation between shoot dry weight of growing plant and fruit yield of pepper plant.