• Title/Summary/Keyword: Height control

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The Use of Styrofoam Box for Chikso (Korean Brindled Cattle) Semen Cryopreservation with Liquid Nitrogen (칡소 동결 정액 생산을 위한 스티로폼상자와 액체질소 이용 방법)

  • Kim, Sung Woo;Ko, Yeoung-Gyu;Lee, Jae-Yeong;Kim, Chan-Lan;Hwang, In-Sul
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.21 no.4
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    • pp.490-496
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    • 2020
  • A styrofoam box is used as a simple and easy freezing method to preserve animal semen as a livestock genetic source. This study optimized the methods of freezing chikso brindled cattle semen. To test the freezing box, the motility of spermatozoa was compared between two box sizes (length×width×heigh) with the dimensions of 23.5×30.5×22.5 cm and 25.5×46.5×26.5 cm. The motility of thawed sperm from brindled Korean bulls was used to confirm the efficiency of the freezing boxes. The box having a larger inner space with larger horizontal and height measurements supported better motility after thawing (60.4±5.3% vs 67.2±3.1%) with 10 min of exposure time in liquid nitrogen vapor. The optimized freezing space is estimated to be an essential element for successful freezing results and the larger box could be used for production of more than 60 frozen semen straws. These properties are also helpful to optimize the cryopreservation techniques that would control the quality and quantity of semen straws according to different animal species.

AN EXPERIMENTAL STUDY ON THE CYTOTOXICITY OF CAVITY LINERS (이장재의 세포독성에 관한 실험적 연구)

  • Kim, Jeong-Hye;Kim, Yung-Hai
    • Restorative Dentistry and Endodontics
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    • v.15 no.2
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    • pp.77-92
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    • 1990
  • The purpose of this study was to evaluate the cytotoxic effects of 6 cavity liners in vitro. Human fibroblasts were cultured in ${\alpha}$-MEM and each liner was manually mixed and filled in glass ring cylinder ($8{\times}8mm$ in diameter, in height). The cylinders filled with the liners were placed in the center of the dish (35mm in diameter) containing 3ml of ${\alpha}$-MEM. Millipore filters (pore size $0.22{\mu}m$) to simulate dentin barrier were also placed between the bottom of cylinder and the dish. Then the culture dishes were stored in 5% $CO_2$ containing incubator for 5 and 10 days at the temperature of $36.6^{\circ}C$. The results of the experiments were analyzed by counting the cells in the period of 5 and 10 days respectively, and were assessed by calculating the cell multiplication rate and the relative growth rate. The experiemntal groups and the control group were compared statistically. The results of the study were summarized as follows: 1. The cell number of Zinc oxide-eugenol was $(4.13{\pm}1.31){\times}10^4$ cells/ml at 5 days and $(4.32{\pm}1.61){\times}10^4$ cells/ml at 10 days. 2. The cell number of Cavitec was ($8.35{\pm}2.87{\times}10^4$ cells/ml and $(10.08{\pm}5.10){\times}10^4$ cells/ml at 5 and 10 days respectively. 3. The cell number of Dycal was $(13.56{\pm}3.89){\times}10^4$ cells/ml at 5 days and $(34.75{\pm}8.85){\times}10^4$ cells/ml at 10 days. 4. The cell number of life was $(11.46{\pm}3.32){\times}10^4$ cells/ml and $(21.92{\pm}6.18){\times}10^4$ cells/ml at 5 and 10 days. 5. The cell number of Base cement was $(13.73{\pm}3.73){\times}10^4$ cells/ml and $(36.68{\pm}5.20){\times}10^4$ cells/ml at 5 and 10 days. 6. The cell number of Dentin cement was $(13.58{\pm}3.90){\times}10$ cells/ml and $(66.95{\pm}24.09){\times}10$ cells/ml at 5 and 10 days. 7. The cell multiplication rate of zinc oxide-eugenol cements was significantly less than that of the calcium hydroxide and glass ionomer cement. (P < 0.05)

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Quality Characteristics of Muffins Containing Wheat Sprout Powder (밀싹 분말 첨가 머핀의 품질 특성)

  • Park, La-Young
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.5
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    • pp.784-789
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    • 2015
  • This study investigated the quality of muffins prepared with various concentrations of wheat sprout powder. Muffins were prepared by adding 0, 1, 3, 5, and 7% wheat sprout powder to flour of the basic formulation. The weight of muffins increased upon addition of 3~5% wheat sprout powder. Height and volume index of the muffins containing 1~5% wheat sprout powder were higher than those of the control. The pH, lightness, and redness of muffins decreased with 3~5% wheat sprout powder, whereas yellowness and moisture contents increased. The hardness of muffins increased upon addition of wheat sprout powder, but there was no significant difference among the sample groups. The total polyphenol contents and antioxidative activity as measured by 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity of muffins increased as the concentration of wheat sprout powder increased. Muffins containing 1~5% wheat sprout powder showed acceptable sensory properties, including color, flavor, taste, and overall acceptability. Results indicate that the optimal concentration of wheat sprout powder in the muffin formula was 3~5% (w/w).

Effect of Geothermal Water on Germination, Seedling Growth and Development of Vascular Bundle in Rice (온천수가 벼종자의 발아, 유묘생장 및 유관속발달에 미치는 영향)

  • Lee, Dong-Jin;Zamora, Oscar B.;Chae, Je-Cheon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.41 no.1
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    • pp.53-61
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    • 1996
  • Geothermal water contains toxic quantities of sulfur, potassium, sodium, boron, and other toxic elements. These toxic elements can substantially restrict germination and seedling growth in rice plant. Germination percentage, average days required for germination and germination velocity were drastically affected by geothermal water. Binato cultivar has higher germination rate compared to IR58 and Unbong 7. Plant height, root length, leaf number and total dry weight decreased with increased geothermal water concentration. Binato and IR58 showed higher total dry weight than Unbong 7 at 25 percent geothermal water at 15 days after treatment (DAT). Binato and IR58 were relatively more tolerant than Unbong 7 in terms of percentage of leaf damage at 25, 50 and 75% concentration of geothermal water at 10 DAT. The development of large and small vascular bundles decreased with increasing concentration of geothermal water from control to 50% in three rice cultivars.

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Effects of Organic-Matter Application on Weed Occurrence and Growth, Yield of Rice Plants (유기물 시용이 벼의 생육 및 수량과 잡초발생에 미치는 영향)

  • Ahn, Jeong-Koo;Lee, Yun-Sang;Lee, Sang-Young;Lee, Chul-Won;Woo, Sun-Hee
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.57 no.2
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    • pp.182-187
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    • 2012
  • Objective of this research was to evaluate the effects of different organic matter on growth and yield in rice plants and suppression of weed occurrence in paddy soil. The treatments consisted of rice bran, expeller cake, or combination of rye green manure and rice bran without any agrochemical or fertilizer. At 50 days after transplanting (DAT), plant height of rice between conventional practice and expeller cake treatments were similar, while rice bran treatment was inhibited growth of rice during growing period. Also, heading date of rice among the all treatments was not significantly different. Rice yield in expeller cake or rye green manure and rice bran treatments was attained to 91% and 98% of the conventional practice, respectively, while the value in rice bran treatments was just 82%. On the other hand, at 40 DAT of rice plants, occurred major weed species in organic matter treatments were four species, while those in conventional practice were two species. Additionally, the dry weight of weeds in rice bran treatment was significantly inhibited compared to it of expeller cake or combination of rye green manure and rice bran treatments, while the value in rice bran treatment was higher than the conventional practice.

A Study on the Development of a Dam Operation Table Using the Rainfall Matrix (강우 매트릭스를 활용한 댐 운영 조견표 개발에 관한 연구)

  • Jeong, Changsam
    • Journal of Korean Society of Disaster and Security
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    • v.13 no.2
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    • pp.39-51
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    • 2020
  • Recently, flood damage has been increasing in Korea due to frequent local torrential rains caused by abnormal weather conditions. According to the calculation of the recurrence period of torrential rain that occurred in North Chungcheong Province on July 16, 2017, it was estimated that the rainfall frequency in the upper are of Goessan Dam was around 1,524 years, and the highest level of Goesan Dam rose to EL.137.60 meters, leaving only 5 cm of margin until the height of the dam floor (EL.137.65 meters). The Goesan Dam, which operated for 62 years since 1957, needs to be prepared to cope with the increase of floodgate volume in the basin, the development of a single purpose dam for power generation only, and there are no measurement facilities for flood control, so efficient operation methods are needed to secure the safety of residents in upper and lower regions. In this study, a method of dam operation was proposed by constructing a rain matrix for quick decision making in flood prediction, calculating the highest level of dam for each condition in advance, and preparing a survey table, and quickly finding the level corresponding to the conditions in case of a situation.

Effects of Depth and Duration of Flooding on Growth and Yield at Different Growth Stage in Pepper(Capsicum annuum L.);I. Response to Flooding at Seedling Stage (고추(Capsicum annuum L.)의 생육단계별(生育段階別) 침수처리(浸水處理)에 따른 생육반응(生育反應);I. 유묘기(幼苗期) 반응(反應))

  • Guh, Ja-Ock;Kuk, Yong-In
    • Korean Journal of Environmental Agriculture
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    • v.15 no.3
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    • pp.325-334
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    • 1996
  • Pepper plants were flooded at 0, 5, 10 and 15cm at seedling stage under the condition of greenhouse. Treatment of flooding times are 6, 12, 24, 48 and 120 hours. The results obtained are summarized as follows. Plant height, number of leaves, shoot and root fresh weight were not recovered the flooding damages regardless of flooding time and depth. Pepper plant died in flooding depth of 5cm or more for over 48 hours. Plants in fallen leaves were found at more than 5cm depth and 6 hours of floodings. Photosynthesis and respiration rate decreased in the 5cm flooding depth or more for 24 hours. Chlorophyll content and root activity decreased for 12 hours or more at all the flooding depth. Also, diffusion resistance of stomata cell increased as increased flooding time and depth. Diseases occurred remarkably in proportion to the depth and hours of flooding treatment. It was not possible to control the desease by fungicide, also then was no effects of foliar spray of urea. Weight of fruit per plant not decrease by the 12 hours of 0cm and the 6 hours of 5cm flooding but decreased at deeper and longer flooding. Average weight of a fruit increased. The yield could not expected in the depth of 5cm or more for over 48 hours, There was significant positive correlation between all the investigated characteristics of growth and yield. There was, however, negative correlation between number of leaf and diffusion resistance of stomata.

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In Vitro Study Evaluating the Antimicrobial Activity of Vancomycin-Impregnated Cement Stored at Room Temperature in Methicillin-Resistant Staphylococcus aureus (상온에서 보관한 반코마이신 함유 항생제 시멘트의 메티실린 내성 포도상구균에 대한 항균력 조사)

  • Park, Se-Jin;Cho, Yongun;Lee, Seok Won;Woo, Hee-Yeon;Lim, Sang Eun
    • Journal of Korean Foot and Ankle Society
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    • v.22 no.1
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    • pp.38-43
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    • 2018
  • Purpose: Treatment of diabetic foot infection due to methicillin-resistant Staphylococcus aureus (MRSA) remains challenging. Applying vancomycin-impregnated cement is one of the best methods of treatment. Vancomycin-impregnated cement has been used worldwide; however, to date, there is a limited number of studies regarding its use. We evaluated the duration of antimicrobial activity of vancomycin-impregnated cement stored at room temperature after manufacturing. Materials and Methods: The vancomycin-impregnated cement was manufactured by mixing 1 g of vancomycin with 40 g of polymer and adding 17.90 g of liquid monomer. The cement dough was shaped into flat cylinders with diameter and height of 6 mm and 2 mm, respectively. Another cement of the same shape without mixing vancomycin was prepared as the negative control. All manufactured cements were sterilized with ethylene oxide gas and stored at room temperature. Each cement was placed on Mueller Hinton agar plate lawned with standard MRSA strain. Standard vancomycin disk and gentamicin disk were placed together. After 24 hours, the diameter of inhibition zone was measured, and if the diameter was less than 15 mm, vancomycin-impregnated cement was regarded as a loss of antimicrobial activity. The study was repeated every 2 weeks until vancomycin-impregnated cements lost their antimicrobial activity. Results: Vancomycin-impregnated cement stored for a duration of 16 weeks created a 14 mm inhibition zone, while vancomycin disk created a 15 mm inhibition zone. Vancomycin-impregnated cement stored for a duration of 17 weeks created 7 mm and 9 mm inhibition zones, while vancomycin disk created 16 mm and 15 mm inhibition zones, respectively. Conclusion: We found a decrease of antimicrobial activity in vancomycin-impregnated cements after 16 weeks. After 17 weeks, they showed definite loss of antimicrobial activity. Therefore, we recommend not using vancomycin-impregnated cement spacers that has been stored for more than 16 weeks at room temperature.

Leaching behavior of the herbicide bentazon in soil column (Soil column중 제초제 bentazon의 용탈 행적)

  • Kyung, Kee-Sung;Oh, Kyeong-Seok;Ahn, Ki-Chang;Kwon, Jeong-Wook;Lee, Jae-Koo
    • The Korean Journal of Pesticide Science
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    • v.2 no.1
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    • pp.59-64
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    • 1998
  • In order to elucidate the leaching behaviour of the herbicide bentazon in soil, soil columns(5cm ID ${\times}$ 34 cm L) were packed with three different soils up to 30 cm height, followed by the treatment of [$^{14}C$]bentazon, and rice plants(Oryza sativa L.) were grown for 9 weeks on these columns, with the columns without growing rice plants as the control for comparison. The amounts of $^{14}C$ activities percolated were about 92% of the originally applied $^{14}C$ irrespective of the physicochemical properties of the soils in the absence of rice plants, whereas $21{\sim}50%$ of the originally applied $^{14}C$ was percolated in the presence of rice plants, suggesting that the amounts of $^{14}C$ leached decreased remarkably in rice-cultivating soils. Bentazon leached faster in soil with higher pH and with lower organic matter content in the presence of growing rice plants. The amounts of $^{14}CO_{2}$ evolved from the soil columns were less than 0.2% of the originally applied $^{14}C$. Smaller amounts of $^{14}C$ were translocated into shoots via roots in soils with higher organic matter content. $^{14}C$ activities distributed into the aqueous phase of the leachate collected from the soil columns increased with leaching period and by rice cultivation, whereas the physico-chemical properties of soils did not exhibit any effect. The amounts of soil-bound residues increased remarkably by cultivating rice plants.

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Comparison of Topographic Surveying Results using a Fixed-wing and a Popular Rotary-wing Unmanned Aerial Vehicle (Drone) (고정익 무인항공기(드론)와 보급형 회전익 무인항공기를 이용한 지형측량 결과의 비교)

  • Lee, Sungjae;Choi, Yosoon
    • Tunnel and Underground Space
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    • v.26 no.1
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    • pp.24-31
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    • 2016
  • Recently, many studies have been conducted to use fixed-wing and rotary-wing unmanned aerial vehicles (UAVs, Drones) for topographic surveying in open-pit mines. Because the fixed-wing and rotary-wing UAVs have different characteristics such as flight height, speed, time and performance of mounted cameras, their results of topographic surveying at a same site need to be compared. This study selected a construction site in Yangsan-si, Gyeongsangnam-do, Korea as a study area and compared the topographic surveying results from a fixed-wing UAV (SenseFly eBee) and a popular rotary-wing UAV (DJI Phantom2 Vision+). As results of data processing for aerial photos taken from eBee and Phantom2 Vision+, orthomosaic images and digital surface models with about 4 cm grid spacing could be generated. Comparisons of the X, Y, Z-coordinates of 7 ground control points measured by differential global positioning system and those determined by eBee and Phantom2 Vision+ revealed that the root mean squared errors of X, Y, Z-coordinates were around 10 cm, respectively.