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http://dx.doi.org/10.5762/KAIS.2020.21.4.490

The Use of Styrofoam Box for Chikso (Korean Brindled Cattle) Semen Cryopreservation with Liquid Nitrogen  

Kim, Sung Woo (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA)
Ko, Yeoung-Gyu (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA)
Lee, Jae-Yeong (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA)
Kim, Chan-Lan (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA)
Hwang, In-Sul (Animal Biotechnology Division, National Institute of Animal Science, RDA)
Publication Information
Journal of the Korea Academia-Industrial cooperation Society / v.21, no.4, 2020 , pp. 490-496 More about this Journal
Abstract
A styrofoam box is used as a simple and easy freezing method to preserve animal semen as a livestock genetic source. This study optimized the methods of freezing chikso brindled cattle semen. To test the freezing box, the motility of spermatozoa was compared between two box sizes (length×width×heigh) with the dimensions of 23.5×30.5×22.5 cm and 25.5×46.5×26.5 cm. The motility of thawed sperm from brindled Korean bulls was used to confirm the efficiency of the freezing boxes. The box having a larger inner space with larger horizontal and height measurements supported better motility after thawing (60.4±5.3% vs 67.2±3.1%) with 10 min of exposure time in liquid nitrogen vapor. The optimized freezing space is estimated to be an essential element for successful freezing results and the larger box could be used for production of more than 60 frozen semen straws. These properties are also helpful to optimize the cryopreservation techniques that would control the quality and quantity of semen straws according to different animal species.
Keywords
Styrofoam Box; Semen; Cryopreservation; Animal Genetic Resources; Liquid Nitrogen;
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