• 한국태양에너지학회:학술대회논문집
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• 2008.04a
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• pp.166-171
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• 2008

We cannot imagine any more the environment and energy problems are separated from our lives. The various attempts to solve these problems are made all over the world. In this study it was performed to analyze a different heating and cooling load depending on the earth-sheltering method and kind of soils by using TRNSYS 16 as the first step to establish the design guidelines for earth-sheltered architecture, one of the eco-friendly and low energy consuming building types. After performing this simulation, we found the result like this. It is the most lowest load in case of all of walls and roof being earth-sheltered. But considering of the load reduction rate, the effect of earth-sheltering the exterior vertical wall is more efficient for load reduction than the one of earth-sheltering a roof. And we got a lower thermal load in case of a lower heat conductivity of soil. Afterwards we will conduct a further study for boundary condition at earth-sheltered surface and the simulation analysis about the sensitivity variables. The final goal of this study is preparing the design guidelines for earth-sheltered architecture. so we will contribute to building energy saving.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2009.11a
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• pp.335-338
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• 2009

The process of charging an oxidizer in the liquid propellant rocket can divide into the cooling of the oxidizer tank, the high flow charge, the small flow charge, and the replenishment charge for the correction of temperature. The oxidizer of the Naro(KSLV-I) first stage uses the liquid oxygen. And the flow rate and the temperature specification corresponding to each charge mode are presented for the requirement. The flow throttling valve and heat exchanger are installed in the oxidizer filling system in order to satisfy this kind of the flow rate and temperature requirement specification. In this research, by using the Flowmaster which is a commercial one-dimension thermo-fluidic analysis program, one dimensional flow system analyses was performed to predict the exact flow rate at each specific mode. Also, the flow rate correction sensitivity of the flow control valves was analytically determined to satisfy the flow condition refinement at each mode within the limited certification test.

• Proceeding of Spring/Autumn Annual Conference of KHA
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• 2009.04a
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• pp.278-283
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• 2009

This study aims to figure out the old people's needs for modification of their housing to maintain an independent lifestyle despite their health status and living arrangements. The total of 438 residents take part in the questionnaire survey research through the quota sampling method grouped by age (60-64 group, 65-69 group, 70-74 group, and over 75 group), sex (male and female), and house type (apartment houses and others). The results are as follows. (1) The old people's most inspired modification needs in interior spaces are remodeling the heating controls in the living room and the bedroom, ventilation facilities and storage spaces in the kitchen, non-slip tile flooring and ventilation facilities in bathroom, an easy door-lock, non-slip tile flooring, a draft cut-off, and storage spaces in the entrance. Besides they require emergency alarm, easy door and window locks, fire and gas alarm, and furniture with easy handling. It is necessary to supply the aged with the appropriate heating controls for their sensitivity to heat, with enough storage spaces for the increased possessions, and with diverse safety systems reflected blunting of mobility and sensibility. (2) As they grow older, the aged require more remote controls and safety facilities such as emergency alarm, easy locks and furniture with distinguishable colors. Male elderly is more concerned with safety, while female elderly do with convenience due to their different time spending in the house. The elderly residents in the apartment houses require the heating controls, a draft cut-off, and storage space less than other types of houses. Thus modification of the heating controls, a draft cut-off, and storage space are regards as basic needs for the elderly residents in non-apartment houses.

• Journal of Environmental Health Sciences
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• v.29 no.2
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• pp.94-102
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• 2003

This study was carried out to investigated the distribution and characteristics of Vibrio spp. isolated from fish and shellfish, seawater and brackish water samples collected from Pohang, Uljin, Yeongduk and Gyeongju in Gyeongbuk Province from April 2000 to October 2000. Total 155 strains of genus Vibrio were isolated from 439 collected samples, and numbers of isolated strains of V. parahaemolyticus and V. vulnificus were 140 and 15, respectively. The isolation rate from the samples collected in Pohang was the highest as 41.5% (76 strains), and wat the highest as 71.4% (30 strains) in brackish water, and was the highest as 55.7% (34 strains) in August. And the optimal pH, temperature, and NaCl concentration for growth of V. parahaemolyticus and V. cholerae were 8.0, 3$0^{\circ}C$ and 2.0％, respectively. In a resistance test for environmental factors, heat and cold resistants of V. parahaemolyticus were higher than those of V. vulnificus, withstanding for 15 minutes at 6$0^{\circ}C$ and 6 days at -18$^{\circ}C$. The pH range for existence of V. parahaemolyticus and V. vulnificus were 4.5~l1.0 and 4.5~10.0, showing the similar resistance to pH. V. parahaemolyticus and V. vulnificus could grow in media containing up to 10.0% and 7.0% NaCl, respectively, Salt-tolerance of V. parahaemolyticus was higher than that of V. vulnificus. In an antibiotics sensitivity test against 16 strains of V. parahaemolyticus, twelve strains were resistant to ampicillin, eight strains were resistant to cephalothin. one strain was resistant to streptomycin, and one strain was resistant to ticarcillin.

• Atmosphere
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• v.26 no.3
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• pp.435-444
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• 2016

In spite of considerable progress in the recent decades, there still remain large uncertainties in numerical cloud models. In this study, effects of uncertainty in terminal velocity of graupel on cloud simulation are investigated. For this, a two-dimensional bin microphysics cloud model is employed, and deep convective clouds are simulated under idealized environmental conditions. In the sensitivity experiments, the terminal velocity of graupel is changed to twice and half the velocity in the control experiment. In the experiment with fast graupel terminal velocity, a large amount of graupel mass is present in the lower layer. On the other hand, in the experiment with slow graupel terminal velocity, almost all graupel mass remains in the upper layer. The graupel size distribution exhibits that as graupel terminal velocity increases, in the lower layer, the number of graupel particles increases and the peak radius in the graupel mass size distribution decreases. In the experiment with fast graupel terminal velocity, the vertical velocity is decreased mainly due to a decrease in riming that leads to a decrease in latent heat release and an increase in evaporative cooling via evaporation, sublimation, and melting that leads to more stable atmosphere. This decrease in vertical velocity causes graupel particles to fall toward the ground easier. By the changes in graupel terminal velocity, the accumulated surface precipitation amount differs up to about two times. This study reveals that the terminal velocity of graupel should be estimated more accurately than it is now.

• Journal of the Korean Society for Nondestructive Testing
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• v.17 no.4
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• pp.262-269
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• 1997

An eddy current probe ($8{\times}1$ multiple-element, surface scan) was successfully designed and fabricated at the KEPRI using the impedance equivalent circuit theory. The probe is intended for the detection of circumferential deformations (cross-section view) of the heat exchanger tubing that can occur due to corrosion, erosion, and denting. Optimum design parameters providing the highest sensitivity and signal-to-noise ratio, such as the coil dimensions, electrical characteristics, and test frequencies, were determined based on initial laboratory experiments conducted on the test specimen (SS304 tubing: OD : 9.68mm, wall-thickness : 0.47mm) containing artificial flaws (e.g., dents and corroded surface on tube OD) using the available Zetec-made probe. Using this parameters, a new probe was made and tested on an unknown specimen. The result indicated that the new probe is capable of detecting the circumferential deformation with the error of ${\pm}0.2%$ (0.022mm) of the tube O.D.

• Food Science of Animal Resources
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• v.37 no.3
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• pp.464-468
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• 2017

The identification of pork in commercially processed meats is one of the most crucial issues in the food industry because of religious food ethics, medical purposes, and intentional adulteration to decrease production cost. This study therefore aimed to develop a method for the detection of pork adulteration in meat products using primers specific for pig mitochondrial DNA. Mitochondrial DNA sequences for pig, cattle, chicken, and sheep were obtained from GenBank and aligned. The 294-bp mitochondrial DNA D-loop region was selected as the pig target DNA sequence and appropriate primers were designed using the MUSCLE program. To evaluate primer sensitivity, pork-beef-chicken mixtures were prepared as follows: i) 0% pork-50% beef-50% chicken, ii) 1% pork-49.5% beef-49.5% chicken, iii) 2% pork-49% beef-49% chicken, iv) 5% pork-47.5% beef-47.5% chicken, v) 10% pork-45% beef-45% chicken, and vi) 100% pork-0% beef-0% chicken. In addition, a total of 35 commercially packaged products, including patties, nuggets, meatballs, and sausages containing processed chicken, beef, or a mixture of various meats, were purchased from commercial markets. The primers developed in our study were able to detect as little as 1% pork in the heat treated pork-beef-chicken mixtures. Of the 35 processed products, three samples were pork positive despite being labeled as beef or chicken only or as a beef-chicken mix. These results indicate that the developed primers could be used to detect pork adulteration in various processed meat products for application in safeguarding religious food ethics, detecting allergens, and preventing food adulteration.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.17
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• pp.7043-7048
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• 2014

Inhibition of heat shock protein 90 (Hsp90) leads to inappropriate processing of proteins involved in DNA damage repair pathways after DNA damage and may enhance tumor cell radio- and chemotherapy sensitivity. To investigate the potentiation of antitumor efficacy of lidamycin (LDM), an enediyne agent by the Hsp90 inhibitorgeldanamycin (GDM), and possible mechanisms, we have determined effects on ovarian cancer SKOV-3, hepatoma Bel-7402 and HepG2 cells by MTT assay, apoptosis assay, and cell cycle analysis. DNA damage was investigated with H2AX C-terminal phosphorylation (${\gamma}H2AX$) assays. We found that GDM synergistically sensitized SKOV-3 and Bel-7402 cells to the enediyne LDM, and this was accompanied by increased apoptosis. GDM pretreatment resulted in a greater LDM-induced DNA damage and reduced DNA repair as compared with LDM alone. However, in HepG2 cells GDM did not show significant sensitizing effects both in MTT assay and in DNA damage repair. Abrogation of LDM-induced $G_2/M$ arrest by GDM was found in SKOV-3 but not in HepG2 cells. Furthermore, the expression of ATM, related to DNA damage repair responses, was also decreased by GDM in SKOV-3 and Bel-7402 cells but not in HepG2 cells. These results demonstrate that Hsp90 inhibitors may potentiate the antitumor efficacy of LDM, possibly by reducing the repair of LDM-induced DNA damage.

• 한국지구물리탐사학회:학술대회논문집
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• 2003.11a
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• pp.244-251
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• 2003

A two-dimensional BEM code, $FRACOD^{2D}$, was applied to simulate fracture initiation and propagation processes in a rock pillar during an in situ heater test of a rock pillar planned at the $\"{A}sp\"{o}$ Underground Rock laboratory of SKB, in Southern Sweden. To take the advantage of conventional BEM for simulating fracturing processes, but without efforts for domain integral transformation, a hybrid approach is developed to simulate the fracturing processes in rock pillar under coupled thermo-mechanical loading. The code FRACOD was used for simulating the fracture initiation and propagation processes with its boundary tractions reflecting the effects of the initial and redistributed thermomechanical stresses in the domain of interest at multiple excavation and heating steps were produced by a special algorithm of stress inversion, based on resultant thermo-mechanical stress fields at each excavation and heat loading step by a FEM code without considering fracturing processes. This hybrid approach can take the advantages of both types of numerical methods and avoids their shortcomings for fracturing process simulation and domain effects, respectively. In this paper, we present the hybrid approach for the stress, displacements, and fracturing processes at sequential excavation and heating steps of the in situ heater test as a predictive modelling, the formulation of the fracturing models and the predictive results. Two sections of borehole depth, 0.5 m and 1.5 m below the tunnel floor are considered. The pillar area is modelled with the FRACOD and the stress field produced by excavation and heating is transferred with corresponding boundary stresses. From the modelling results, the degree of fracturing and damage are evaluated for 120 days of heating. Dominated shear fracturing in the vicinity of the central pillar was observed from the models at both sections, but spalled area appears to be limited. Based on the modelling results, a sensitivity study for the effect of pre-existing fractures in the vicinity of the holes is also conducted, and the initiation and evolution of EDZ around the deposition holes are investigated using this particular numerical technique.

• The Plant Pathology Journal
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• v.19 no.5
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• pp.260-265
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• 2003

Gummy stem blight caused by Didymella bryoniae occurs exclusively in cucurbits. This fungus has been known not to produce its pycnidium in vitro unless irradiated. In this study, cultural conditions for the mass-production of pycnidiospore by Metal Halide (MH) lamp irradiation were maximized. The mycelia were cultured at $26^{\circ}C$ on PDA for 2 days under dark condition, and then the plate was illuminated with MH lamp continuously for 3-4 days at $26^{\circ}C$. Results show that a great number of pycnidia were simultaneously formed. The pycnidiospores produced were then used as immunogen. Fusions of myeloma cell (v-653) with splenocytes from immunized mice were carried out. Two hybridoma cell lines that recognized the immunogen D. bryoniae were obtained. One monoclonal antibody (MAb), Dbl, recognized the supernatant while another MAb, Db15, recognized the spore. Two clones were selected which were used to produce ascite fluid of the two MAb, Dbl and Db15, the immunotypes of which were identified as IgG1 and IgG2b, respectively. Titers of MAb Dbl and MAb Db15 were measured and the absorbance exceeded 0.5 even at a $10^{-5}$ dilution. The MAbs reacted positively with D. bryoniae but none reacted with other viral isolates, Cucumber mosaic virus and Cucumber green mottle mosaic virus. Sensitivity of MAb was precise enough to detect spore concentration as low as $10^{-3}$/well by indirect ELISA. Characterization of the MAbs Dbl, Db15 antigen by heat and protease treatments, which suggests that the epitope recognized by these two MAbs was glycoprotein.