• Title/Summary/Keyword: Heat exchange

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Studies on the Rice Yield Decreased by Ground Water Irrigation and Its Preventive Methods (지하수 관개에 의한 수도의 멸준양상과 그 방지책에 관한 연구)

  • 한욱동
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.16 no.1
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    • pp.3225-3262
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    • 1974
  • The purposes of this thesis are to clarify experimentally the variation of ground water temperature in tube wells during the irrigation period of paddy rice, and the effect of ground water irrigation on the growth, grain yield and yield components of the rice plant, and, furthermore, when and why the plant is most liable to be damaged by ground water, and also to find out the effective ground water irrigation methods. The results obtained in this experiment are as follows; 1. The temperature of ground water in tube wells varies according to the location, year, and the depth of the well. The average temperatures of ground water in a tubewells, 6.3m, 8.0m deep are $14.5^{\circ}C$ and $13.1^{\circ}C$, respercively, during the irrigation period of paddy rice (From the middle of June to the end of September). In the former the temperature rises continuously from $12.3^{\circ}C$ to 16.4$^{\circ}C$ and in the latter from $12.4^{\circ}C$ to $13.8^{\circ}C$ during the same period. These temperatures are approximately the same value as the estimated temperatures. The temperature difference between the ground water and the surface water is approximately $11^{\circ}C$. 2. The results obtained from the analysis of the water quality of the "Seoho" reservoir and that of water from the tube well show that the pH values of the ground water and the surface water are 6.35 and 6.00, respectively, and inorganic components such as N, PO4, Na, Cl, SiO2 and Ca are contained more in the ground water than in the surface water while K, SO4, Fe and Mg are contained less in the ground water. 3. The response of growth, yield and yield components of paddy rice to ground water irrigation are as follows; (l) Using ground water irrigation during the watered rice nursery period(seeding date: 30 April, 1970), the chracteristics of a young rice plant, such as plant height, number of leaves, and number of tillers are inferior to those of young rice plants irrigated with surface water during the same period. (2) In cases where ground water and surface water are supplied separately by the gravity flow method, it is found that ground water irrigation to the rice plant delays the stage at which there is a maximum increase in the number of tillers by 6 days. (3) At the tillering stage of rice plant just after transplanting, the effect of ground water irrigation on the increase in the number of tillers is better, compared with the method of supplying surface water throughout the whole irrigation period. Conversely, the number of tillers is decreased by ground water irrigation at the reproductive stage. Plant height is extremely restrained by ground water irrigation. (4) Heading date is clearly delayed by the ground water irrigation when it is practised during the growth stages or at the reproductive stage only. (5) The heading date of rice plants is slightly delayed by irrigation with the gravity flow method as compared with the standing water method. (6) The response of yield and of yield components of rice to ground water irrigation are as follows: \circled1 When ground water irrigation is practised during the growth stages and the reproductive stage, the culm length of the rice plant is reduced by 11 percent and 8 percent, respectively, when compared with the surface water irrigation used throughout all the growth stages. \circled2 Panicle length is found to be the longest on the test plot in which ground water irrigation is practised at the tillering stage. A similar tendency as that seen in the culm length is observed on other test plots. \circled3 The number of panicles is found to be the least on the plot in which ground water irrigation is practised by the gravity flow method throughout all the growth stages of the rice plant. No significant difference is found between the other plots. \circled4 The number of spikelets per panicle at the various stages of rice growth at which_ surface or ground water is supplied by gravity flow method are as follows; surface water at all growth stages‥‥‥‥‥ 98.5. Ground water at all growth stages‥‥‥‥‥‥62.2 Ground water at the tillering stage‥‥‥‥‥ 82.6. Ground water at the reproductive stage ‥‥‥‥‥ 74.1. \circled5 Ripening percentage is about 70 percent on the test plot in which ground water irrigation is practised during all the growth stages and at the tillering stage only. However, when ground water irrigation is practised, at the reproductive stage, the ripening percentage is reduced to 50 percent. This means that 20 percent reduction in the ripening percentage by using ground water irrigation at the reproductive stage. \circled6 The weight of 1,000 kernels is found to show a similar tendency as in the case of ripening percentage i. e. the ground water irrigation during all the growth stages and at the reproductive stage results in a decreased weight of the 1,000 kernels. \circled7 The yield of brown rice from the various treatments are as follows; Gravity flow; Surface water at all growth stages‥‥‥‥‥‥514kg/10a. Ground water at all growth stages‥‥‥‥‥‥428kg/10a. Ground water at the reproductive stage‥‥‥‥‥‥430kg/10a. Standing water; Surface water at all growh stages‥‥‥‥‥‥556kg/10a. Ground water at all growth stages‥‥‥‥‥‥441kg/10a. Ground water at the reproductive stage‥‥‥‥‥‥450kg/10a. The above figures show that ground water irrigation by the gravity flow and by the standing water method during all the growth stages resulted in an 18 percent and a 21 percent decrease in the yield of brown rice, respectively, when compared with surface water irrigation. Also ground water irrigation by gravity flow and by standing water resulted in respective decreases in yield of 16 percent and 19 percent, compared with the surface irrigation method. 4. Results obtained from the experiments on the improvement of ground water irrigation efficiency to paddy rice are as follows; (1) When the standing water irrigation with surface water is practised, the daily average water temperature in a paddy field is 25.2$^{\circ}C$, but, when the gravity flow method is practised with the same irrigation water, the daily average water temperature is 24.5$^{\circ}C$. This means that the former is 0.7$^{\circ}C$ higher than the latter. On the other hand, when ground water is used, the daily water temperatures in a paddy field are respectively 21.$0^{\circ}C$ and 19.3$^{\circ}C$ by practising standing water and the gravity flow method. It can be seen that the former is approximately 1.$0^{\circ}C$ higher than the latter. (2) When the non-water-logged cultivation is practised, the yield of brown rice is 516.3kg/10a, while the yield of brown rice from ground water irrigation plot throughout the whole irrigation period and surface water irrigation plot are 446.3kg/10a and 556.4kg/10a, respectivelely. This means that there is no significant difference in yields between surface water irrigation practice and non-water-logged cultivation, and also means that non-water-logged cultivation results in a 12.6 percent increase in yield compared with the yield from the ground water irrigation plot. (3) The black and white coloring on the inside surface of the water warming ponds has no substantial effect on the temperature of the water. The average daily water temperatures of the various water warming ponds, having different depths, are expressed as Y=aX+b, while the daily average water temperatures at various depths in a water warming pond are expressed as Y=a(b)x (where Y: the daily average water temperature, a,b: constants depending on the type of water warming pond, X; water depth). As the depth of water warning pond is increased, the diurnal difference of the highest and the lowest water temperature is decreased, and also, the time at which the highest water temperature occurs, is delayed. (4) The degree of warming by using a polyethylene tube, 100m in length and 10cm in diameter, is 4~9$^{\circ}C$. Heat exchange rate of a polyethylene tube is 1.5 times higher than that or a water warming channel. The following equation expresses the water warming mechanism of a polyethylene tube where distance from the tube inlet, time in day and several climatic factors are given: {{{{ theta omega (dwt)= { a}_{0 } (1-e- { x} over { PHI v })+ { 2} atop { SUM from { { n}=1} { { a}_{n } } over { SQRT { 1+ {( n omega PHI) }^{2 } } } } LEFT { sin(n omega t+ { b}_{n }+ { tan}^{-1 }n omega PHI )-e- { x} over { PHI v }sin(n omega LEFT ( t- { x} over {v } RIGHT ) + { b}_{n }+ { tan}^{-1 }n omega PHI ) RIGHT } +e- { x} over { PHI v } theta i}}}}{{{{ { theta }_{$\infty$ }(t)= { { alpha theta }_{a }+ { theta }_{ w'} +(S- { B}_{s } ) { U}_{w } } over { beta } , PHI = { { cpDU}_{ omega } } over {4 beta } }}}} where $\theta$$\omega$; discharged water temperature($^{\circ}C$) $\theta$a; air temperature ($^{\circ}C$) $\theta$$\omega$';ponded water temperature($^{\circ}C$) s ; net solar radiation(ly/min) t ; time(tadian) x; tube length(cm) D; diameter(cm) ao,an,bn;constants determined from $\theta$$\omega$(t) varitation. cp; heat capacity of water(cal/$^{\circ}C$ ㎥) U,Ua; overall heat transfer coefficient(cal/$^{\circ}C$ $\textrm{cm}^2$ min-1) $\omega$;1 velocity of water in a polyethylene tube(cm/min) Bs ; heat exchange rate between water and soil(ly/min)

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Radiation, Energy, and Entropy Exchange in an Irrigated-Maize Agroecosystem in Nebraska, USA (미국 네브라스카의 관개된 옥수수 농업생태계의 복사, 에너지 및 엔트로피의 교환)

  • Yang, Hyunyoung;Indriwati, Yohana Maria;Suyker, Andrew E.;Lee, Jihye;Lee, Kyung-do;Kim, Joon
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.22 no.1
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    • pp.26-46
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    • 2020
  • An irrigated-maize agroecosystem is viewed as an open thermodynamic system upon which solar radiation impresses a large gradient that moves the system away from equilibrium. Following the imperative of the second law of thermodynamics, such agroecosystem resists and reduces the externally applied gradient by using all means of this nature-human coupled system acting together as a nonequilibrium dissipative process. The ultimate purpose of our study is to test this hypothesis by examining the energetics of agroecosystem growth and development. As a first step toward this test, we employed the eddy covariance flux data from 2003 to 2014 at the AmeriFlux NE1 irrigated-maize site at Mead, Nebraska, USA, and analyzed the energetics of this agroecosystem by scrutinizing its radiation, energy and entropy exchange. Our results showed: (1) more energy capture during growing season than non-growing season, and increasing energy capture through growing season until senescence; (2) more energy flow activity within and through the system, providing greater potential for degradation; (3) higher efficiency in terms of carbon uptake and water use through growing season until senescence; and (4) the resulting energy degradation occurred at the expense of increasing net entropy accumulation within the system as well as net entropy transfer out to the surrounding environment. Under the drought conditions in 2012, the increased entropy production within the system was accompanied by the enhanced entropy transfer out of the system, resulting in insignificant net entropy change. Drought mitigation with more frequent irrigation shifted the main route of entropy transfer from sensible to latent heat fluxes, yielding the production and carbon uptake exceeding the 12-year mean values at the cost of less efficient use of water and light.

A study on the origination and transmission of Koh(袴) in Northeast Asia-from the 4th century to 7th century (동북아세아(東北亞細亞) 고(袴)의 발생(發生) 및 전파(傳播)에 관(關)한 연구(硏究) - $4{\sim}7$세기(世紀) 중심으로 -)

  • Park, Kyung-Ja;Lee, Jean-Kyung
    • Journal of the Korean Society of Costume
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    • v.15
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    • pp.177-194
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    • 1990
  • Koh(袴) was a type of dress worn on the lower part of the body which was commonly used in the Northeast Asia. It was originally used by the Northern race for the need of nomadism or hunting. The origin of the Koh which appeared in the area would be found from the trousers of the Huns who influenced in the Northeast Asia, and became in the part of the Scythian culture. The Scythians are the nomadizing race inhabited in the Northern Caucasas on the wast of the Black Sea and influenced on the inland Eurasian steppe as the first typical horse-riding race. The objectives of Koh which had been worn in the Scythian, Mongolia, Korea as well as Japan as a part of Dongho dress and ornaments and to contemplate the transmission process by cultural exchange among different races for the period from 4th century to 7th century. 1. The Origination of the Koh The Koh was originated by the environmental factor to protect the cold in the North but also from the heat in the South, and was changed and developed as gradually satisfying to the needs of the times. In the Northeast Asia the Koh was in the class of the Northern Chinese garment, and was used widely by the horse riding Scythians who moved widely from the Eurasian inland to Japan. The oldest original which could reflect the type of the Northern clothes was a pair of trousers discovered in the Huns remains of Noin Ula. This showed the exact form of hunting clothes and had a similar form with the Korean female tro-users. Since the same form of trousers drawn on the wall painting of which was excavated 4-5th century ancient Koguryo(高句麗) tomb was the same form the trousers of Noin Ula seemed to be the original form of Koh in the Northeast Asia. 2. The Chinese Trousers It was the time of the King Mooryung(武靈王) in the Cho(趙) Dynasty B.C. 3th century that the trousers used regularly in China. However, the Koh had been used as undergarment which functioned for the protection of the cold not the horseriding garment. The trousers seemed to be not very obviously shown off since the Poh (袍) was long, but mainly used by the people from lower class. As people learned the adapted the trousers. It was essential for the times of war and quarrel. The king himself started wearing the Koh. The Chinese trousers were influenced by the Huns, the Northern clothes of the Scythian culture, and similar to the Korean clothes. 3. The Korean Trousers Korean was a race bared from the Eastern foreign group. It was obvious that the clothes was Baji-Jeogori(바지 저고리), the garment of the Northern people. This had the same form of the Scythian dress and ornaments which was excavated from the Mongolian Noin Ula. The Scythian dress and ornaments were influenced from the Ancient West Asia Empire and transmitted to the Northeast Koguryu by the horseriding Scythian. The trousers were kept in the traditional style by the common people in Korea were transmitted to Japan which were for behind in cultural aspect, as well as got used to the Chinese as the efficient clothes though active cultural exchange. 4. The Japanese Trousers The ancient Japanese clothes were influenced by the Southern factor but not the form of the Koh. As the Korean people group was moving towards Japan and conquer the Japanese in the 4-5th century, however, North Altaic culture was formed and at the same time the clothes were also developed. The most influenced clothes at this time were those of Baekge(百濟) and the trousers form called Euigon became the main form. Because of the climatic regional factor, it was tied not at the ankle but under the knee. From the view the ancient Japanese clothes disappeard about that time, it could be due to the conquest of the culturally superior race but not the transmission of the culture. In the latest 7th century both the Chinese and Japanese dress forms were present, but the Dongho(東胡) dress and its ornament from Korea was still the basic of the Japanese dress form.

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Development of Assay Methods for Enterotoxin of Escherichia coli Employing the Hybridoma Technology (잡종세포종기법을 이용한 대장균의 장독소 측정법 개발)

  • Kim, Moon-Kyo;Cho, Myung-Je;Park, Kyung-Hee;Lee, Woo-Kon;Kim, Yoon-Won;Choi, Myung-Sik;Park, Joong-Soo;Cha, Chang-Yong;Chang, Woo-Hyun;Chung, Hong-Keun
    • The Journal of the Korean Society for Microbiology
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    • v.21 no.1
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    • pp.151-161
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    • 1986
  • In order to develop sensitive and sepcific assay methods for E. coli heat labile enterotoxin(LT) hybridoma cell lines secreting LT specific monoclonal antibody were obtained. LT was purified from cell lysate of E. coli O15H11. The steps included disruption of bacteria by French pressure, DEAE Sephacel ion exchange chromatography, Sephadex G200 gel filtration, and second DEAE Sephacel ion exchange chromatography, successively. Spleen cells from Balb/c mice immunized with the purified LT and $HGPRT^{(-)}$ plasmacytomas, $P3{\times}63Ag8.V653$ were mixed and fused by 50% (w/v) PEG. Hybrid cells were grown in 308 wells out of 360 wells, and 13 wells out of them secreted antibodies reacting to LT. Among these hybridoma cell 1G8-1D1 cell line was selected since it had produced high-titered monoclonal antibody continuously. By using culture supernatant and ascites from 1G8-1D1 cells the monoclonal antibody was characterized, and an assay system for detecting enterotoxigenic E. coli was established by double sandwich enzyme-linked immunosorbent assay (ELISA). The following results were obtained. 1. Antibody titers of culture supernatant and ascites from 1G8-1D1 hybridoma cells were 512, and 102, 400, respectively by GM1-ELISA and its immunoglobulin class was IgM. 2. The maximum absorption ratio of 1G8-1D1 cell culture supernatant to LT was 90% at $300\;{\mu}g/ml$ of LT concentration. LT concentration shown at 50% absorption ratio was $103.45{\mu}g$ and the absorption ratio was decreased with tile reduction of LT concentration. This result suggests that monoclonal antibody from 1G8-1D1 hybridoma cell bound with LT specifically. 3. The reactivities of 1G8-1D1 cell culture supernatant to LT and V. cholerae enterotoxin(CT) were 0.886 and 0.142(O.D. at 492nm) measured by the GM1-ELISA, indicating 1G8-1D1 monoclonal antibody reacted specifically with LT but not with CT. 4. The addition of 0.1ml of ascites to 0.6mg and 0.12mg of LT decreased the vascular permeability factor to 41% and 44% respectively, but it did not completely neutralize LT. 5. By double sandwich ELISA using monoclonal antibody, as little as 75ng of the purified LT per ml could be detected. 6. The results by assay of detecting LT in culture supernatants of 14 wild strains E. coli isolated from diarrhea patients by the double sandwich ELISA were almost the same level as those by reverse passive latex agglutination.

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Effect of Coolant on PEMFC Performance in Low Humidification Condition (저가습 조건에서 냉각 유체의 고분자전해질 연료전지에 대한 영향)

  • Lee, Hung-Joo;Song, Hyun-Do;Kwon, Jun-Taek;Kim, Jun-Bom
    • Journal of the Korean Electrochemical Society
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    • v.10 no.1
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    • pp.25-30
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    • 2007
  • Proton exchange membrane fuel cell(PEMFC) performance could be affected by various factors such as cell temperature, total pressure, partial pressure of reactants and relative humidity. Hydrogen ion is combined with water to form hydronium ion [$H_3O^+$] and pass through membrane resulting electricity generation. Cooling system is needed to remove heat and other uses on large scale fuel cell. In case that collant conductivity is increased, fuel cell performance could be decreased because produced electricity could be leaked through coolant. In this study, triple distilled water(TDW) and antifreeze solution containing ethylene glycol was used to observe resistance change. Resistance of TDW was taken 28 days to reach preset value, and effect on fuel cell operation was not observed. Resistance of antifreeze solution was not reached to preset value up to 48 days, but performance failure occurred presumably caused by bipolar plate junction resulting stoppage resistance experiment. Generally PEMFC humidification is performed near-saturated operating conditions at various temperatures and pressures, but non-humidifying condition could be applied in small scale fuel cell to improve efficiency and reduce system cost. However, it was difficult to operate large scale fuel cell without humidifying, especially higher than $50{\sim}60^{\circ}C$. In case of small flux such as 0.78 L/min, temperature difference between inlet and outlet was occurred larger than other cases resulting performance decrease. Non-humidifying performance experiments were done at various cell temperature. When both of anode and cathode humidification were removed, cell performance was strongly depended on cell operating temperature.

Control of Water-Adsorption Properties of Mesoporous Silica and MOF by Ion Exchange and Salt Impregnation (양이온 교환 및 염 함침을 통한 메조다공성 실리카와 유기-금속 구조체의 수분 흡착 특성 조절)

  • Lee, Eun Kyung;Cho, Kanghee;Kim, Sang Kyum;Lim, Jong Sung;Kim, Jong-Nam
    • Clean Technology
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    • v.24 no.1
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    • pp.55-62
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    • 2018
  • The adsorbent used in water-adsorption cooling system utilizing low-temperature heat of below $90^{\circ}C$ is required to exhibit high water uptake capacity at a relative humidity ($P/P_0$) between 0.1 and 0.3. Mesoporous silica (MCM-41) and MOF(MIL-101) exhibit quite large water adsorption capacity under saturated water vapor at $35^{\circ}C$. However, these adsorbents show small water adsorption capacity ($0.027{g_{water}\;g_{ads}}^{-1}$, $0.074{g_{water}\;g_{ads}}^{-1}$, respectively) in the relative humidity ($P/P_0$) range of 0.1 to 0.3. In this study, the surface properties of mesoporous silica and MOF were modified by simple methods to develop an adsorbent having a higher water uptake than the conventional water adsorbents at a relative humidity ($P/P_0$) of 0.1 ~ 0.3. In the case of mesoporous silica (MCM-41) exhibiting mainly water adsorption at $P/P_0=0.5{\sim}0.7$, aluminum species was functionalized on the mesopore walls and then cations existing near the aluminum were exchanged with various cations (e.g., $Na^+$, ${NH_4}^+$, and $(C_2H_5)_4N^+$). In addition, 20 wt% (to total weight of the composites) of hygroscopic inorganic salt ($CaCl_2$) was impregnated on the MCM-41. In the case of the MIL-101 (MOF), 20 wt% of hygroscopic inorganic salt ($CaCl_2$) was impregnated on the MIL-101. The MCM-41 which was ion-exchanged with various cations has main adsorption branch around 0.5 of $P/P_0$ which was slightly shifted with low-pressure direction in comparison with pristine MCM-41. However, tiny increases were observed on the adsorption in the range of $P/P_0$ between 0.1 and 0.3. After salt impregnation on the MCM-41, the adsorption capacity under $P/P_0=0.1{\sim}0.3$ at $35^{\circ}C$ was increased from $0.027{g_{water}\;g_{ads}}^{-1}$ to $0.152{g_{water}\;g_{ads}}^{-1}$. In the case of MIL-101, the amount of water adsorption at $35^{\circ}C$ under $P/P_0=0.1{\sim}0.3$ was increased from $0.074{g_{water}\;g_{ads}}^{-1}$ to $0.330{g_{water}\;g_{ads}}^{-1}$ after the salt impregnation.

Large-scale Virtual Power Plant Management Method Considering Variable and Sensitive Loads (가변 및 민감성 부하를 고려한 대단위 가상 발전소 운영 방법)

  • Park, Yong Kuk;Lee, Min Goo;Jung, Kyung Kwon;Lee, Yong-Gu
    • Journal of the Institute of Electronics and Information Engineers
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    • v.52 no.5
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    • pp.225-234
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    • 2015
  • Nowadays a Virtual Power Plant (VPP) represents an aggregation of distributed energy resource such as Distributed Generation (DG), Combined Heat and Power generation (CHP), Energy Storage Systems (ESS) and load in order to operate as a single power plant by using Information and Communication Technologies, ICT. The VPP has been developed and verified based on a single virtual plant platform which is connected with a number of various distributed energy resources. As the VPP's distributed energy resources increase, so does the number of data from distributed energy. Moreover, it is obviously inefficient in the aspects of technique and cost that a virtual plant platform operates in a centralized manner over widespread region. In this paper the concept of the large-scale VPP which can reduce a error probability of system's load and increase the robustness of data exchange among distributed energy resources will be proposed. In addition, it can directly control and supervise energy resource by making small size's virtual platform which can make a optimal resource scheduling to consider of variable and sensitive load in the large-scale VPP. It makes certain the result is verified by simulation.

Production of Ammonia Water and Sulfuric Acid from Ammonium Sulfate by Electrodialysis with a Bipolar Membrane (바이폴라막 전기투석을 이용한 황산암모늄으로부터 암모니아수와 황산의 제조)

  • Hwang, Ui-Son;Choi, Jae-Hwan
    • Journal of Korean Society of Environmental Engineers
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    • v.27 no.1
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    • pp.36-42
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    • 2005
  • This study examined the feasibility of producing sulfuric acid and ammonia water from ammonium sulfate solution using two-compartment electrodialysis with a bipolar membrane (EDBM). Electrodialysis experiments were carried out with 20 wt% ammonium sulfate at different current densities and sulfuric acid concentrations in a concentrate compartment. The current efficiency increased with the current density from 25 to $100\;mA/cm^2$. Nevertheless, the efficiency was relatively low compared with that of general desalting electrodialysis, owing to the diffusion of sulfuric acid from the concentrate compartment to the diluate. The diffusion rate through the anion exchange membrane increased with the sulfuric acid concentration in the concentrate compartment, which decreased the current efficiency. Conversely, the electrical resistance decreased with increasing current density owing to the Joulian heat generated during water dissociation in the transition region of the bipolar membrane under a high electric field. From the experimental results, we concluded that operating at a higher current density is effective from the perspective of current efficiency and electrical resistance when producing sulfuric acid and ammonia water from ammonium sulfate using a two-compartment EDBM process. Further studies on the effects of increasing the sulfuric acid concentration on current efficiency are required to apply the EDBM process practically.

Separation of Soybean Protein by Free-flow Electrophoresis (자유유동 전기이동법에 의한 대두단백질 분리)

  • 한재갑;류화원
    • KSBB Journal
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    • v.10 no.1
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    • pp.63-70
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    • 1995
  • The effect of operating conditions on separation of soybean proteins in a home-made free-flow electrophoresis apparatus was investigated. Measurement of the pH, conductivity, and UV-absorbance(280 nm) were carried out at each run and the purity of the sample was tested with SDS-PAGE analysis. The soybean extract pretreated with Tris and boric acid was mixed with the amino acids composed of glutamic acid, histidine, arginine, glycine(1 mM each) with glycyl-glycine(2mM) and KCl(1mM). When the cellulose acetate was used as a compartment between the electrode and the buffer solution in the cell, pH distribution in the separation cell varied from 3.0 at the anodic side to 8.0 at the cathodic side and had two inflection point. The applied voltage was from 300V to 1000V and the separation was better at a higher voltage but the voltage was limited by the capability of the cooling system due to Joule heat. The proteins focused near the middle of the channel. From the change of pH and conductivity it was found that the ions in the channel moved out to the electrodes through the membrane. In the case when the concentration of the buffer solution was increased 5 times, proteins were focused at 300V. We could not increase up to the ten times of the concentration since the temperature difference between inlet and outlet was more than $25^{\circ}C$ and denaturation of proteins was expected. When ion-exchange membranes were used U-type pH distribution was set up due to the ionic polarization near the membrane. The commercial ampholytes, instead of the mixed amino acids showed not much improvements in purity of the separated sample.

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Design of Energy Model of Greenhouse Including Plant and Estimation of Heating and Cooling Loads for a Multi-Span Plastic-Film Greenhouse by Building Energy Simulation (건물에너지시뮬레이션을 활용한 연동형 온실 및 작물에너지모델 설계 및 이의 냉·난방부하 산정)

  • Lee, Seung-No;Park, Se-Jun;Lee, In-Bok;Ha, Tae-Hwan;Kwon, Kyeong-Seok;Kim, Rack-Woo;Yeo, Uk-Hyeon;Lee, Sang-Yeon
    • Journal of Bio-Environment Control
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    • v.25 no.2
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    • pp.123-132
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    • 2016
  • The importance of energy saving technology for managing greenhouse was recently highlighted. For practical use of energy in greenhouse, it is necessary to simulate energy flow precisely and estimate heating/cooling loads of greenhouse. So the main purpose of this study was to develope and to validate greenhouse energy model and to estimate annual/maximum energy loads using Building Energy Simulation (BES). Field experiments were carried out in a multi-span plastic-film greenhouse in Jeju Island ($33.2^{\circ}N$, $126.3^{\circ}E$) for 2 months. To develop energy model of the greenhouse, a set of sensors was used to measure the greenhouse microclimate such as air temperature, humidity, leaf temperature, solar radiation, carbon dioxide concentration and so on. Moreover, characteristic length of plant leaf, leaf area index and diffuse non-interceptance were utilized to calculate sensible and latent heat exchange of plant. The internal temperature of greenhouse was compared to validate the greenhouse energy model. Developed model provided a good estimation for the internal temperature throughout the experiments period (coefficients of determination > 0.85, index of agreement > 0.92). After the model validation, we used last 10 years weather data to calculate energy loads of greenhouse according to growth stage of greenhouse crop. The tendency of heating/cooling loads change was depends on external weather condition and optimal temperature for growing crops at each stage. In addition, maximum heating/cooling loads of reference greenhouse were estimated to 644,014 and $756,456kJ{\cdot}hr^{-1}$, respectively.