• Journal of Life Science
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• v.14 no.4
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• pp.683-688
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• 2004

A bacteriocin-producing lactic acid bacteria was isolated from Kimchi on MRS selective media with the use of Lactobacillus delbrueckii subsp. delbrueckii as an indicator strain. The strain YH-10 was identified as Lactococcus lactis subsp. lactis through the API test. The crude bacteriocin (freeze-dried 50% ammonium sulfate precipitate of culture supernatant) produced by the strain was named as lacticin YH-10. Lacticin YH-10 showed the growth inhibitory activity against Gram positive pathogenic bacteria and other lactic acid bacteria. The bacteriocin was inactivated by proteases such as protamex and aroase AP-10 and partially inactivated by amylase, proteinase K, trypsin, and papain. The lacticin YH-10 remained its activity with the treatment of heat at 10$0^{\circ}C$ for 60 min or the changes of pH 2 to 11. However, the activity was lost at high pH combined with the exposure to 10$0^{\circ}C$. The bacteriocin production of the strain was started in the exponential phase and stopped in the stationary phase. The approximate molecular mass of the bacteriocin produced by the strain was approximate 14 kDa in the analysis on SDS-PAGE.

• Journal of the Korean Geotechnical Society
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• v.29 no.5
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• pp.29-38
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• 2013

The G-class cement is commonly used in practice for geothermal well cementing in order to protect a steel casing that is designed to transport hot water/steam from deep subsurface to ground surface during operating a geothermal power plant. In order to maintain optimal performance of geothermal wells, physical properties of the cementing material should be satisfactory. In this paper, relevant factors (i.e., groutability, uniaxial compression strength, thermal conductivity and free fluid content) of the G-class cement were experimentally examined with consideration of various water-cement (w/c) ratios. Important findings through the experiments herein are as follows. (1) Groutability of the G-class cement increases by adding a small dose of retarder. (2) There would be a structural defect caused when the w/c ratio is kept higher in order to secure groutability. (3) Thermal conductivity of the G-class cement is small enough to prevent heat loss from hot steam or water to the outer ground formation during generating electricity. (4) The G-class cement does not form free water channel in cementing a geothermal well. (5) The Phenolphthalein indicator is applicable to the distinction of the G-class cement from the drilling mud.

• Journal of the Korean Society of Marine Environment & Safety
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• v.25 no.7
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• pp.898-905
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• 2019

As the positive feedback between the absorption of chromophoric dissolved organic matter (CDOM) and acceleration of ice melt can impact the aquatic biota and dynamic heat budget, long-term monitoring of the CDOM variation in the polar ocean is necessary. However, the monitoring of CDOM is not easy because of harsh weather and difficult access, especially in the Antarctic Ocean. Therefore, the purpose of this study was to find a suitable long-term monitoring site for CDOM variation; we selected Maxwell Bay and Marian Cove at Sejong Base and horizontal and vertical distributions of CDOM were measured. After a 72 hr time-series measurement test of the CDOM variation at Sejong Dock and Sejong Cape in Maxwell Bay, Sejong Dock was selected, as it does not haveland discharge effects. The seasonal variation of CDOM was evident and the average CDOM concentration of Maxwell Bay was comparable with the adjacent sea. The CDOM at Sejong Dock from February to November 2010 was the highest in the fall and winter and the lowest during spring and summer. Thus, based on our one-year CDOM data, we suggest that Sejong Dock in Maxwell Bay is suitable for long-term monitoring of CDOM as an indicator of photochemical and biological environmental change and an important factor in determining the heating budget in the Antarctic Ocean.

• Journal of Climate Change Research
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• v.4 no.1
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• pp.1-10
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• 2013

This study has purpose to minimize the impact of climate change of Siheung. Vulnerability assessment was carried out for establishing the Siheung Climate Change Master Plan. Climate change vulnerability assessment analyzed using climate exposure, sensitivity and adaptive capacity indicators. A proxy variable is selected from each indicator. Meteorological data uses the RCP scenarios provided by the Meteorological Administration, and this study assumes that the same trend will continues in the future. Siheung are vulnerable to heavy rains in the flooded roads and farmland. Also, it is necessary to be careful heat wave in summer. The size and scale of the damage depends on the city's ability to respond to the impacts of climate change. It is necessary to make a adaptation plan for climate change impact assessment and vulnerability analysis. This study will be used to make Siheung Climate Change Master Plan and to determine the priority of the policy as guideline. It is expected that this study is helpful to pursue climate change vulnerability assessment of other local governments.

• Journal of the Korean Institute of Landscape Architecture
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• v.47 no.5
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• pp.28-40
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• 2019

In order to resolve the imbalances in the supply of living SOCs according to socio-economic status, location, and population groups, the discussions on inclusive city policies are expanding. The purpose of this study is to propose an Index of Park Derivation (IPD) as an alternative indicator for the promotion of an inclusive urban park policy that can be applied in the 7 major metropolitan cities to select a region with a relatively high park needs. The main research results are as follows. First, the concept of an inclusive urban park policy is defined as "a policy to supply to manage high-quality park services with priority given to areas with low socio-economic and environmental status, such as a large amount of elderly, children, low-income families, areas vulnerable to disasters, such as heat and fine dust, and population groups." Second, we developed the index of park derivation (IPD), which is a combination of 17 variables including park service level, demographic characteristics, economic and educational level, health level, and environmental vulnerability. The variables that constitute the index of park deprivation (IPD) can be applied to SOC policies outside the parks, such as sports facilities, daycare centers, kindergartens, and public libraries. Third, applying index of park deprivation (IPD) to 1,148 Eup/Myeon/dong areas of the 7 metropolitan cities resulted in areas with relatively high park service needs. This study implies that the central and the local government suggest an alternative index to promote an inclusive urban park policy based on statistical and geographical information and data that can be easily accessed and utilized.

• Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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• 2001.06a
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• pp.1610-1610
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• 2001

Microbiological examination of silage is of little value in gauging the outcome of silage, and so chemical analysis is more reliable and meaningful indicator of quality. On the other hand chemical assessments of the principal fermentation products provide an unequivocal basis on which to judge quality. Livestock require energy, protein, minerals and vitamins from their food. While fresh forages provide these essential items, conserved forages on the other hand may be deficient in one or more of them. The aim of the conservation process is to preserve as many of the original nutrients as possible, particularly energy and protein components (Woolford, 1984). Silage fermentation is important to preservation of forage with respect of feeding value and animal performance. Chemical and bacteriological changes in the silo during the fermentation process can affect adversely nutrient yield and quality (Moe and Carr, 1984). Many of the important chemical components of silage must be assayed in fresh or by extraction of the fresh material, since drying either by heat or lyophilisation, volatilises components such as acids or nitrogenous components, or effects conversion to other compounds (Abrams et al., 1987). Maize silage dorms the basis of winter rations for the vast majority of dairy and beef cattle production in Uruguay. Since nutrient intake, particularly energy, from forages is influenced by both voluntary dry matter intake and digestibility; there is a need for a rapid technique for predicting these parameters in farm advisory systems. Near Infrared Reflectance Spectroscopy (NIRS) is increasingly used as a rapid, accurate method of evaluating chemical constituents in cereals and dried forages. For many years NIRS was applied to assess chemical composition in dry materials (Norris et al., 1976, Flinn et al., 1992; Murray, 1993, De Boever et al., 1996, De la Roza et al., 1998). The objectives of this study were (1) to determine the potential of NIRS to assess the chemical composition of dried maize samples and (2) to attempt calibrations on undried samples either for farm advisory systems or for animal nutrition research purposes in Uruguay. NIRS were used to assess the chemical composition of whole - plant maize silage samples (Zea mays, L). A representative population of samples (n = 350) covering a wide distribution in chemical characteristics were used. Samples were scanned at 2 nm intervals over the wavelength range 400-2500 nm in a NIRS 6500 (NIRSystems, Silver Spring, MD, USA) in reflectance mode. Cross validation was used to avoid overfitting of the equations. The optimum calibrations were selected on the basis of minimizing the standard error of cross validation (SECV). The calibration statistics were R$^2$ 0. 86 (SECV: 11.4), 0.90 (SECV: 5.7), 0.90 (SECV: 16.9) for dry matter (DM), crude protein (CP), acid detergent fiber (ADF) in g kg$\^$-1/ on dry matter, respectively for maize silage samples. This work demonstrates the potential of NIRS to analyse whole - maize silage in a wide range of chemical characteristics for both advisory farm and nutritive evaluation.

• International Journal of Industrial Entomology and Biomaterials
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• v.46 no.1
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• pp.16-23
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• 2023

The silkworm's dormancy and embryonic development are accomplished through the interaction of various genes. Analysis of the expression of several interacting genes can predict the embryonic stage of silkworms. In this study, we analyzed the changes in the expression level of genes at each stage during the embryonic development of dormant silkworm eggs and selected genes that can predict the hatching time. Jam123 and Jam124 silkworms were collected after egg laying, and the silkworm eggs were preserved using a double refrigeration method and expression analysis was performed for 23 genes during embryogenesis. There were 5 genes showing significant changes during embryogenesis: UDP-glucuronosyltransferases (BmUGTs), heat shock protein hsp20.8 (BmHsp20.8), Cytochromes b5-like proteins (BmCytb5), Krüppel homolog 1 (BmKr-h1), and cuticular protein RR-1 motif 41 (BmCpr41). As a result of quantitative comparison of the expression levels of these 5 genes through real-time PCR, the BmUGTs gene showed a difference between Jam123 and Jam124, making it difficult to see it as an indicator for predicting hatching time. However, the BmHsp20.8 gene had a common expression decreased at the imminent hatching stage. In addition, it was confirmed that the expression level of the BmCytb5 gene decreased to the lowest level at the time of imminent hatching, and the expression of the BmKr-h gene was made only at the time of imminent hatching. The expression of the last BmCpr41 gene can be confirmed only at the time of imminent hatching, and it was confirmed that it shows a rapid increase right before hatching. Taken together, these results suggest that expression analysis of BmHsp20.8, BmCytb5, BmKr-h1, and BmCpr41 genes can determine the stage of embryogenesis, predict hatching time, which facilitate better management of silkworm eggs.

• Annals of Occupational and Environmental Medicine
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• v.34
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• pp.23.1-23.14
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• 2022

Background: The musculoskeletal disease (MSD) burden is an important health problem among Korean fishers. We aimed to investigate the indicators of the prevalence of MSD and contributions of significant indicators to MSD in Korean fishers. Methods: This cross-section study included 927 fishers (male, 371; female, 556) aged 40 to 79 years who were enrolled from 3 fishery safety and health centers. The outcome variable was one-year prevalence of MSD in 5 body parts (the neck, shoulder, hand, back, and knee). Independent variables were sex, age, educational attainment, household income, job classification, employment xlink:type, hazardous working environment (cold, heat, and noise), ergonomic risk by the 5 body parts, anxiety disorder, depression, hypertension, diabetes, and hyperlipidemia. The adjusted odds ratio of MSDs by the 5 body parts were calculated using multiple logistic regression analysis. We computed the population attributable fraction (PAF) for each indicators of MSDs using binary regression models. Results: The one-year prevalence of MSD in the neck, shoulder, hand, back, and knee was 7.8%, 17.8%, 7.8%, 27.2%, and 16.2% in males vs. 16.4%, 28.1%, 23.0%, 38.7%, and 30.0% in females, respectively. The ergonomic risk PAF according to the body parts ranged from 22.8%-59.6% in males and 22.8%-50.3% in female. Mental diseases showed a significant PAF for all body parts only among female (PAF 9.1%-21.4%). Cold exposure showed a significant PAF for the neck, shoulder, and hand MSD only among female (25.6%-26.8%). Age was not a significant indicator except for the knee MSD among female. Conclusions: Ergonomic risk contributed majorly as indicators of MSDs in both sexes of fishers. Mental disease and cold exposure were indicators of MSDs only among female fishers. This information may be important for determining priority risk groups for the prevention of work-related MSD among Korean fishers.

• Korean Journal of Environment and Ecology
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• v.28 no.1
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• pp.80-89
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• 2014

This study of the school's outdoor space for relaxing and activity of the two most numerous students, high heat and low heat with a green space, playground targets of thermal comfort indicators UTCI, PMV, WBGT using the thermal comfort students feeling compare the analyzed. The destination of this study, school facilities of Nam-gu, Namdong- gu, Incheon were studied and the investigation period was conducted from July to August. List of measurement, in the case of thermal comfort indicators, UTCI, PMV, WBGT was measured in the case of green, ratio of green coverage and GVZ was measured. GVZ analysis were as follows: A school ($4.71m^3/m^2$) B school ($3.34m^3/m^2$) C school ($0.38m^3/m^2$). Comparative analysis of the results of thermal comfort indicators by schools, UTCI was Green space $26.15{\sim}31.38^{\circ}C$ and playground $40.66{\sim}42.94^{\circ}C$, PMV values were 1.76 to 2.66 as a green space. WBGT was Green space $26.15{\sim}31.38^{\circ}C$, playground $31.67{\sim}34.53^{\circ}C$. Comparative analysis of the results of thermal comfort indicators UTCI, PMV, WBGT all A school, B school, C school, on the green space was comfortable levels more than playground. The results of the school type thermal comfort and green correlation analysis of thermal comfort UTCI, PMV, WBGT all solar radiation, globe temperature, and a positive correlation shown solar radiation, globe temperature is not comfortable, the higher was considered. UTCI, PMV, WBGT of thermal comfort indicator all ratio of green coverage, GVZ and negative correlation appears ratio of green coverage, GVZ was increased due to the lowering of the value of thermal comfort indicators was considered to be comfortable.

• Journal of Plant Biotechnology
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• v.43 no.3
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• pp.359-366
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• 2016

Tradescantia is a perennial plant in the family of Commelinaceae. It is known to be sensitive to radiation. In this study, Tradescantia BNL 4430 was irradiated with gamma radiation at doses of 50 to 1,000 mGy in a phytotron equipped with a $^{60}Co$ radiation source at Korea Atomic Energy Research Institute, Korea. At 13 days after irradiation, we extracted RNA from irradiated floral tissues for RNA-seq. Transcriptome assembly produced a total of 77, 326 unique transcripts. In plantlets exposed to 50, 250, 500, and 1000 mGy, the numbers of up-regulated genes with more than 2-fold of expression compared that in the control were 116, 222, 246, and 308, respectively. Most of the up-regulated genes induced by 50 mGy were heat shock proteins (HSPs) such as HSP 70, indicating that protein misfolding, aggregation, and translocation might have occurred during radiation stress. Similarly, highly up-regulated transcripts of the IQ-domain 6 were induced by 250 mGy, KAR-UP oxidoreductase 1 was induced by 500 mGy, and zinc transporter 1 precursor was induced by 1000 mGy. Reverse transcriptase (RT) PCR and quantitative real time PCR (qRT-PCR) further validated the increased mRNA expression levels of selected genes, consistent with DEG analysis results. However, 2.3 to 97- fold higher expression activities were induced by different doses of radiation based on qRT-PCR results. Results on the transcriptome of Tradescantia in response to radiation might provide unique identifiers to develop in situ monitoring kit for measuring radiation exposure around radiation facilities.