• Applied Biological Chemistry
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• v.6
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• pp.61-77
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• 1965

In this experiment, Akiochi was studied especially on plant growth on the degraded soils. Besides, such soils were carefully examined on its character and plant body was analysed to know the difference in various mineral contents. For this purpose, paddy cultivation was done with the variety Pal Dal at Suwon, Sosa and Pyungtak. Three plots were chosen at each location as the normal and 2 levels of akiochi, a-the stronger and b-the weaker. Harvests from these 9 plots were measured agronomically and also chemically analysised. As for soil, after an observation on vertical section of soil, samples from each layer were also studied both physically and chemically. The results are summarized as follows. 1. Outer changes in rice plant and changes in yield components. 1) Rice from Akiochi soil showed remarkably shortened culm length, head length, protrusoion length, blade length of boot leaf, and coleoptile length, compared with that from the normal paddy field. 2) There was a tendency for Akiochi rice to have more heads per plant. 3) Akiochi rice showed poorer intercalary growth of upper 3 internodes. The ratio of this upper internode length to total culm length was also smaller in this case. Consquently the ratio of lower internode length to total culm length became larger than that from normal peddy field. 4) Akiochi rice showed significantly fewer first spikelets and attached grains of head at main stem. 5) Maturing rate of both this main seem of whole plant body was remarkably lower than that of normal rice. 6) Akiochi rice showed lower head weight of main stem, total hulled rice weight, total grain yield, 1000-grain weight, straw weight and straw-hulled rice ratio. 2. Physical and chemical study on soil. 1) Akiochi soil showed thinner upper layer and total thickness of upper and lower parts was smaller than that of normal. 2) Akiochi soil of Suwon was mainly composed of sand, while that of Sosa and Pyungtak was composed of heavy clay. 3) Chemical analysis indicated that content of $SiO_2$ in upper layer is always lower than that of normal. But no other common tendencies were found. 4) This analysis further lillustrates lower content of Fe, & Mn at Suwon ; of Mn at Sosa and higher content of Fe at Sosa and organic matters at Pyungtak. 5) Some differences in the content of N in each plot could be marked though irregular. 3. Chemical Composition of plant body. 1) Chemical analysis on grain, boot leaf and straw did not suggest any remarkable differences between normal and Akiochi rice, except that the latter contains less Si in boot leaf and less Mn in straw. 2) Contents of each chemical element were measured in grain and straw to calculate the percentage of element content in grain to that of whole plant body including both grain and straw. Here, Akiochi rice always showed lower value in N, K and Mn. 4. Relationship between chemical composition of plant body and that of soil. Akiochi soil at Sosa marked lower content of Mn. This caused another lower content of this element in grain, boot leaf and straw. But except that, no remarkable relationship could be found in this study.

• Polymer(Korea)
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• v.39 no.3
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• pp.493-498
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• 2015

For biomaterials for skin regeneration with minimized inflammatory response, high bioactivity and biocompatibility are highly required. Also, it should have a porous microstructure to improve cell adhesion and growth. In this study, we extracted a new collagen source from duck's feet which is by-product, and made the shape of sponges from duck's feet collagen (DC) to compare with DBP and SIS. To analyze physical and chemical property of the scaffold, SEM and FTIR were used. MTT assay was used to measure the attachment and proliferation of NIH/3T3 in the scaffolds. RTPCR was used to evaluate the expression of proinflammatory cytokine. Also, 1,1-diphenyl-2-picrylhydrazyl (DPPH) was used to measure the ability of antioxidant activity. Overall, this study shows that DC scaffold is biocompatible and has good physical property. Additionally, DC scaffold shows the potential as wound healing biomaterials.

• Journal of Animal Science and Technology
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• v.62 no.5
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• pp.668-681
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• 2020

The purpose of this study was aimed to isolate a Salmonella Typhimurium-specific phage (KFS-ST) from washing water in a poultry processing facility and to investigate the feasibility of the KFS-ST as a novel bio-receptor for the magnetoelastic (ME) biosensor method. KFS-ST against S. Typhimurium was isolated, propagated, and purified using a CsCl-gradient ultracentrifugation. Morphological characteristics of KFS-ST were analyzed using transmission electron microscopy (TEM). Its specificity and efficiency of plating analysis were conducted against 39 foodborne pathogens. The temperature and pH stabilities of KFS-ST were investigated by the exposure of the phage to various temperatures (-70℃-70℃) and pHs (1-12) for 1 h. A one-step growth curve analysis was performed to determine the eclipse time, latent time and burst size of phage. The storage stability of KFS-ST was studied by exposing KFS-ST to various storage temperatures (-70℃, -20℃, 4℃, and 22℃) for 12 weeks. KFS-ST was isolated and purified with a high concentration of (11.47 ± 0.25) Log PFU/mL. It had an icosahedral head (56.91 ± 2.90 nm) and a non-contractile tail (225.49 ± 2.67 nm), which was classified into the family of Siphoviridae in the order of Caudovirales. KFS-ST exhibited an excellent specificity against only S. Typhimurium and S. Enteritidis, which are considered two of the most problematic Salmonella strains in the meat and poultry. However, KFS-ST did not exhibit any specificity against six other Salmonella and 27 non-Salmonella strains. KFS-ST was stable at temperature of 4℃ to 50℃ and at pH of 4 to 12. The eclipse time, latent time, and burst size of KFS-ST were determined to be 10 min, 25 min and 26 PFU/ infected cell, respectively. KFS-ST was relatively stable during the 12-week storage period at all tested temperatures. Therefore, this study demonstrated the feasibility of KFS-ST as a novel bio-receptor for the detection of S. Typhimurium and S. Enteritidis in meat and poultry products using the ME biosensor method.

• Korean journal of applied entomology
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• v.36 no.4
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• pp.317-322
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• 1997

Hatched-larvae of the mulberry longicorn beetle, Apriona germari Hope, collected from mulberry fields were reared on artificial diet at 25$^{\circ}$C with 14 h light and 10 h dark to study the larval developmental characteristics. Artificial diet developed for rearing silkworm was used with minor modification adding mulberry branch powder. In case of artificial diet rearing, the head width of larval instar from the I st to the 12th instars was ranged from 0.12 to 0.69cm, and growth rate of each instar was significantly high between the I st and the 2nd instars. In addition, the weight of the 8th instar larvae was increased approximately 176-fold in comparision with that of the 1st instar larvae. Larval duration of each instar took long with larval developmental stages, and that of the 1st to the 9th or the 12th instars was 186.03 or 304.58 days, respectively. The survival rate of larvae was 40.8% by the 8th instar. The pupation rate was approximately 32.4%. Furthermore, although pupation stage was broadly appeared from the 7th to the I lth instars, pupation was majorly observed at the 8th and the 9th instars.

• Korean Journal of Ichthyology
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• v.13 no.3
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• pp.166-172
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• 2001

Parental care of eggs of Chromis fumea (Tanaka) was observed by Skin Scuba gear in August 1995 off Cheju Island. The eggs and larval development were observed in the laboratory at Pukyong National University, Pusan. Eggs were deposited on a flat surface of hard substratum and formed a plain sheet of one layer of eggs. Males showed parental care of the eggs by hovering over the nest and attacking other fishes that approached the nest. The spawned eggs were transparent, elliptical in shape and measured 0.73~0.88 mm in long axis and 0.50~0.56 mm in short axis. An analysis by T-test of the long axis of the egg with time showed a significant difference (p<0.01). However, there was no significant difference in short axis with time. Eggs were reared at water temperatures of $23.0{\sim}25.5^{\circ}C$ and took about 4~5 days from embryonic body stage to hatching. The newly hatched larvae were 1.10~1.61 mm in notochord length (NL) had 21 myomeres, has closed mouth but open anus, preanus length was 45.8% of NL. Melanophores were distributed on the occipital region of head, the eye, the peritoneal region, and along the ventral contour. The larvae absorbed the yolk material and oil globule in 3~4 days after hatching and became postlarvae. On the third day after hatching, the larvae had attained 1.9~2.4 mm in NL, had 25 myomeres, and showed rapid growth. By the ninth day after hatching, the larvae had attained 2.9 mm in NL, had an swimbladder in the dorsal margin of ventral region, the preanus length was 37.9% of NL, and their melanophores were enlarged and reduced in number.

• Korean Journal of Ichthyology
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• v.19 no.4
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• pp.343-349
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• 2007

Embryonic and larval developments on Pacific cod, Gadus macrocephalus are described from laboratory-reared specimens. The eggs (1.075~1.168 mm in diameter) were weakly adhesive, almost spherical in shape, transparent and unpigmented, showing a pale yellow yolk without oil globules. Hatching was occurred 220 hrs after fertilization at $9.8^{\circ}C$. The newly hatched larvae (3.72~4.40 mm in total length, TL) had 43~47 (11~13+32~34) myomeres, mouth and anus which was not yet opened. The yolk was completely absorbed at 4.58 mm TL, and transformed to postlarval stage. Locations of the anus and second dorsal fin origin, and distinctive growth of the larvae head were observed at 5.48 mm TL with melanophores in the gill cover and upper region of the occipital. In addition, melanophores were distinctive in the ventral region and caudal fin base at 6.88 mm TL. All fins were formed with a complete set of fin rays having the following counts (D. 3~7-12~14-13~14; A. 13-15) at 11.54 mm TL. Both lateral line and barbel of lower jaw were observed at 15.07 mm TL.

• Korean Journal of Animal Reproduction
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• v.16 no.1
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• pp.63-76
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• 1992

This study was carried out to investigate the appearance time of the second polar body for producing Gynogenesis or Triploid which could be obtained by arresting the second polar body by cold shock, and then blastoderm was used to measure fertility that revealed the nature of oogenesis, the effects of water temperature on fertility, hatchability, abnormality, viability and growth rate, and the water temperature and the breeding methods to prevent early death of larvae in Korean loach(Misgurnus anguillicaudatus) ; the results obtained in this study were summarized as follows. The second polar body was observed ont he surface of plasma disc close to micropyle within 10~40 min after fertilization at 29$^{\circ}C$. Artificial inseminatin had to be done immediately after the egg spawning because the spermatozoa of loach their mobility within 2 minutes when they were exposed to water. The amount of time needed to reach at blastoderm stage was 12 hours if fertilized eggs were incubated at 16$^{\circ}C$, 8 hours at 19$^{\circ}C$, 6 hours at 21$^{\circ}C$, 5 hours at 23$^{\circ}C$, 4 hours at 26$^{\circ}C$ and 3 hours 30 min at 29$^{\circ}C$ showing the shorter time for development of eggs at higher temperature. Fertilization rates in water temperatures of 19$^{\circ}C$, 21$^{\circ}C$, 23$^{\circ}C$, and 26$^{\circ}C$ were higher than those of water temperatures, 16$^{\circ}C$ and 29$^{\circ}C$. Water temperatures at 19$^{\circ}C$, 21$^{\circ}C$, and 23$^{\circ}C$ showed higher hatching rates that those of 16$^{\circ}C$, 26$^{\circ}C$, and 29$^{\circ}C$, while abnormal rates in 16$^{\circ}C$, 19$^{\circ}C$, 21$^{\circ}C$ and 23$^{\circ}C$ were lower than that of 26$^{\circ}C$ and 29$^{\circ}C$. Water temperatures at 16$^{\circ}C$, 19$^{\circ}C$, 21$^{\circ}C$, 23$^{\circ}C$ and 26$^{\circ}C$ respectively, were more different than 29$^{\circ}C$ in survival rates. The embryos were hatched at 72 hours after fertilization in 16$^{\circ}C$ water temperature, 48 hours in 19$^{\circ}C$, 40 hours in 21$^{\circ}C$, 32 hours in 23$^{\circ}C$, 25 hours in 26$^{\circ}C$, and 16 hours in 29$^{\circ}C$. Within three days after hatched out, the larvage grew 3mm in total length, the yolk granules were entirely consumed and the head and the trunk became thicker. Within 45 days after hatched out, the larva grew 25mm at 29$^{\circ}C$, 21mm at 26$^{\circ}C$, 16mm at 23$^{\circ}C$, 15mm at 21$^{\circ}C$, 12mm at 16$^{\circ}C$ in a 30 litreglass aquarium.

• Korean Journal of Community Nutrition
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• v.11 no.1
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• pp.3-13
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• 2006

The purpose of this study is to estimate the nutrient intakes and food habits among preschool children in Kyungjoo city. The subjects were 210 preschool children, aged 4 - 6 years. Measurements of the weight, height, chest circumference, and head circumference of the children were conducted. And general home environment and factors related to eating habits for preschool children were collected using a questionnaire that included information about family income, parents' education and occupations. The average weight-length index (WLI) for the subjects was $103.9\%$. Using the WLI, $20.0\%$ of the preschool children were underweight, $48.6\%$ were normal, $19.0\%$ were overweight, and $12.3\%$ were obese. On the R$\ddot{o}$hrer index, $13.8\%$ of the preschool children were underweight, $38.5\%$ were normal, and $47.7\%$ were over weight or obese. The average daily intake ($\%$ of RDA) of energy and each nutrient was 1323.5 kcal ($81.3\%$), Ca 484.3 mg ($80.7\%$), Fe 7.05 mg ($88.1\%$), vitamin A 420.0 RE ($105\%$), vitamin $B_{2}$ 0.76 mg ($95.0\%$), vitamin $B_{2}$ 0.87 mg ($87.0\%$), and vitamin C 53.1 mg ($106.2\%$) , respectively. In particular, older subjects had lower intake in RDA $%$ of calcium and iron. The energy intake ratio from snacks was much higher than the recommended level of the preschool children. With regard to frequency of regularity of breakfast, $1.9\%$ of preschool children skipped every morning and $7.6\%$ of the children skipped more than 5 per week. With regard to the intake frequency of vegetables, fruits, complex carbohydrates, and milk, $13.3\%$, $19.9\%$, $22.8\%$, and $41.8\%$ of the children ate more than 5 times per week. The eating habit score was positively correlated (r = 0.18, P < 0.05) with household income. This study suggests that nutrition education to increase fruit and vegetable consumption for preschool children should be emphasized. Also a nutrition education program is needed to enhance consuming calcium and iron intake for adequate growth.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.5
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• pp.440-444
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• 2006

This study was carried out to compare the grain quality under lodging time and grade at ripening. Ripened grain ratio and 1000-grain weight were higher with lodging of plants at $45^{\circ}$ angle from the ground and increased with crop development. The number of spikelets, as well as the protein and amylose content of the grains, were higher in plants under severe or complete lodging than those under lodging at $45^{\circ}$ angle from the ground. Palatability and sensory evaluation values were higher in plants under lodging at $45^{\circ}C$ angle from the ground than those under complete lodging. Brown rice: rough rice ratio was higher with lodging of plants at $45^{\circ}C$ angle from the ground and increased with crop growth. Milling recovery and milled rice recovery were higher with lodging of plants at $45^{\circ}C$ angle from the ground, whereas chalky grain, cracked grain and damage grains were higher at complete lodging.

• Magazine of the Korean Society of Agricultural Engineers
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• v.31 no.2
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• pp.82-91
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• 1989

This study was carried out to get the basic information of irrigation plans for the red pepper, such as optimum irrigation level and irrigation requirement in Taegu and Kyungpook province. In this study, red peppers were cultivated in 6 PVC pot lysimeters filled with 60cm deep clay loam soil. Four tensiometers were installed in each plot to measure the soil water pressure head. Field measurements were made during the period June 6 to October 31, 1988 at the experimental farm of Kvungpook National University. Six levels of irrigation were used. They were PF 1.8-2.0, PF 2.2-2.4, PF 2.8-3.0, FC-PF.1.7, FC-PF 2.2, and FC-PF 2.7. The results obtained from this study are summarized as follows : 1. In case of irrigation levels of narrow ranges of water contents, the higher the soil water content was, the larger the ET was. Hut in case of the irrigation levels returning to the field capacity, the lager the PF value of irrigation point was, the larger the ET was. Considering ET, yield and weight per fruit, the latter is much better than the former irrigation method. 2. The mean daily ET and mean ET ratio for each 10-day period showed that the maximum value occured in the last of August. The ranges of those were 3.74-14.64 mm/day and 0.87-3.40, respectively. These values showed that small during the early stage of growth, large during the middle stage and getting smaller in the last stage. 3. In case of irrigation levels of narrow ranges of water contents, the increase of irrigation water supplied increased the ET. The relationship between the two showed nearly straight line. Most of irrigated water was consumed as ET and the rest as percolation. But, in case of irrigation levels returning to the field capacity, the higher the PF value of irrigation point was, the larger the ET ratio was. However, their relationship didn't show straight line. 4. The irrigation level of PC - PP 2.7 was found to be the optimum irrigation level with respect to the yield, the weight per fruit, stem length, irrigation requirement and percolation quantity. In this case, mean daily ET and mean ET ratio were 6.79 mm/day (total 10052 mm) and 1.67, respectively. The maximum mean daily ET and mean ET ratio for 10-day period were 14.64 mm/day and 3.40, respectively, in the last of August, and the maximum daily ET was 2126 mm/day on August 24. 5. In case of PC - PP 2.7 which is found the optimum irrigation level, mean irrigation water required, mean ET and mean percolation water quantity were 7.44 mm/day, 6.79 mm/day(91.3% of irrigation water), and 0.38 mm/day (5.5% of it), respectively.