• Archives of Pharmacal Research
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• 제2권1호
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• pp.25-33
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• 1979

In order to find out nutritional effects of Korean edible mushrooms on the multiplication of tissue cells, the alcohol extracts and acid bydrolysates of eleven species of mushrooms were added to Earle's ESS. A comparison of the respective multiplications of HeLa cells in this solution and in control solution of TC=199. Yielded the following result: The alcohol extracts and acid hydrolysates of a Coprinus comatur, Agaricus campestris, Agaricus bisporus, Lentinus edodes, Tricholoma matsutake, Pleurotus ostretus, Ramaria botrytis and Pholiota nameko influenced favorably the maintenance of the normal form and monolayer of HeLa cells. The growth curves of HeLa cells in the cultures containing, respectively, the alcohol extracts and acid hydrolysates of these eight mushrooms showed that five species, i.e., Coprinus comatus, Agaricus compestris, Agaricus bisporus, Lentinus edodes and Tricholoma matsutake effected an excellent multiplication and that the other three species were less effective than those five species. As to the effects on the cell multiplication, no marked difference was observed between the alcohol extracts and the acid hydrolysates of the mushrooms tested.

• 환경생물
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• 제23권2호
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• pp.152-156
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• 2005

Ionizing radiation is a well- known therapy factor for human carcinoma cells. Genotoxic stress mediates cell cycle control, transcription and cellular signaling. In this work, we have used a microarray hybridization approach to characterize the cell type-specific transcriptional response of human carcinoma MCF-7 and HeLa cell line to $\gamma-radiation$, such as 4Gy 4hr. We found that exposure to $\gamma-ray$ alters by at least a $log_2$ factor of 1.0 the expression of known genes. Of the 27 genes affected by irradiation, 11 are down- regulated in MCF-7 cells and 2 genes induced by radiation,15 are repressed in HeLa cells. Many genes were involved in known damage- response pathways for cell cycling, transcription factor and cellular signaling response. However, in MCF-7 cells, we observed gene expression pattern in chromatin, apoptosis, stress, differentiation, cytokine, metabolism, ribosome and calcium. In HeLa cells, it showed clearly the expression changes in adhesion and migration, lysosome, brain, genome instability and translation. These insights reveal new therapy directions for studying the human carcinoma cell response to radiation.

• 대한한방부인과학회지
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• 제19권1호
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• pp.14-30
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• 2006

Purpose : This study was conducted to investigate the inhibitory effects of Cortex ulmi pumilae on cell proliferation in HeLa cell. Methods : Human uterine cervical carcinoma HeLa cells were cultured in the 1%, 5% and 10% concentration of Cortex ulmi pumilae solution for 24 hours, 48 hours and 72 hours for the direct inhibitory effects of Cortex ulmi pumilae. Afterwards, we executed the analysis of the effect of Cortex ulmi pumilae solution on cell proliferation inhibition using XTT assay, DNA fragmentation, molecular biological method through MAP kinase activity and FACS analysis of caspase activity in the HeLa cells. Results : After 48 and 72 hours cultivation, the HeLa cells showed the concentration-dependently significant increase in all Cortex ulmi pumilae solution containing groups compared to the control. In the FACS analysis, all Cortex ulmi pumilae solution containing groups showed concentration-dependent increase compared to the control after 24 hours cultivation and the caspase-3 activities were decreased in all Cortex ulmi pumilae solution containing groups compared to the control after 24, 48 and 72 hours cultivation. After 48 and 72 hours cultivation, we could examined the apparent DNA fragmentation in all Cortex ulmi pumilae solution containing groups. In the XTT study, all Cortex ulmi pumilae solution containing groups showed concentration-dependent decrease compared to the control after 24 and 72 hours cultivation but 10% group after 48 hours and 5% and 10% groups after 72hours were presumed statistically significant differences. The expressions of MAP kinase were decreased in all Cortex ulmi pumilae solution containing groups compared to the control after 24, 48 and 72 hours cultivation. Conclusion : From this study we could suggest that Cortex ulmi pumilae be available to the inhibition of apoptosis of human cervical carcinoma cell line in vitro.

• Applied Microscopy
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• 제43권3호
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• pp.117-121
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• 2013

Mitochondrion is an important intracellular organelle controlling energy production essential for cell survival. In addition, it is closely related to cellular apoptosis and necrosis. Linear, branched, circular, and ball-shaped mitochondria have been reported. Recent research suggests that mitochondrial morphology may reflect functional status of the cell. In this study, we investigated the density and ratio of the each morphological categories of mitochondria in a few normal cultured cells; astrocyte, HeLa and COS7 cells, of which metabolic activities are different, with high voltage electron microscopy. The absolute number and relative number per unit area of mitochondria was largest in astrocyte. But, the proportion of different mitochondrial shape was similar among cells. These results shows the numerical profiles but not morphological profiles of mitochondria are related to the metabolic activity of each cell line.

• 한국미생물·생명공학회지
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• 제32권1호
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• pp.72-77
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• 2004

본 연구진은 토양미생물의 배양액으로부터 cyclin-dependent kinase 저해활성의 Toyocamycin을 분리하였으며 〔16〕, 화학적 전합성을 통하여 활성이 개선된 유도체인 신물질 MCS-5A를 합성하였다〔3〕. 이 MCS-5A를 이용한 항암 기전규명을 위한 연구를 통하여 , human promyelocytic leukemia cell(HL-60)에서 MCS-5A에 의해 cyclin-dependent kinase inhibitor p16$^{INK4A}$ 단백질의 발현증가가 암세포의 세포주기 억제와 동시에 HL-60 cell희 세포사멸을 유도하는 것을 확인하였다(data not shown). 그러나 HL-60 cell의 경우와는 달리 non small cell lung cancer cell(NSCLC)인 A549 cell(p16$^{INK4A}$ 결핍 세포주)에 MCS-5A를 처리할 경우에는 전혀 세포사멸이 유도되지 않았다. 따라서 MCS-5A에 의한 HL-60 cell에서의 세포사멸 유도는 발암억제 유전자인 P16$^{INK4A}$의 세포 내 발현 및 존재 여부에 의해 좌우되는 것으로 판단되었다. 이러한 배경에서 본 연구는 p16$^{INK4A}$.의 기존에 알려진 세포주기 억제를 유발하는 cyclin-dependent kinase inhibitor(CKI)로서의 역할 뿐 아니라, p16$^{INK4A}$ 유전자가 세포사멸을 유도할 수 있다는 새로운 기능을 규명하기 위하여 다음의 연구를 시도하였다. 즉 $p^{INK4A}$ 결핍 세포주인 A549(-p16/+p53)와 H1299(-pl6/-p53) 그리고 p16$^{INK4A}$ 함유 세포주인 HeLa(+p16/+p53)세포에 외부로부터 p16$^{INK4A}$ 유전자를 도입시켜, 각 세포주에서의 세포사멸 유도 여부를 비교하고자 하였다. 우선 wild-type p16$^{INK4A}$ 유전자를 가진 HeLa cell에서 총 RNA를 추출하여, 역전사 반응으로 cDNA를 만들고, PCR을 통해 p16$^{INK4A}$ 유전자를 증폭하였다. pcDNA3.1/His is A vector에 p16$^{INK4A}$ 유전자를 끼워 넣고 competent cell (XL1-Blue)에 형질 전환하여 cloning한 후, p16$^{INK4A}$ clone을 다량으로 추출하였다. 위에 언급한 각각의 cell line에 p16$^{INK4A}$유전자를 농도(0, 1, 5, 10$\mu\textrm{g}$)별로 transfection 시킨 후, p16 단백질을 일정 시간 동안(12시간) 발현시킨 뒤, TUNEL등의 분석을 통해 세포사멸이 유도되는지를 확인하였으며, 또한 Western blot 분석을 통하여 p16단백질과 세포사멸 유도 인자인 caspase 3의 발+현 양상을 확인하였다. 연구 결과, Western blot을 통해 transfection시킨 p16/INK4A/유전자의 농도에 따라 각각의 cell line에서 Pro-caspase 3의 감소함을 관찰할 수 있었고, TUNEL분석을 통해 A549및 HeLa cell에서 세포사멸이 유도됨을 확인할 수 있었다 특히 A549(-p16/+p53)와 HeLa cell(+p16/+p53)에서는 TUNEL 분석 및 Western blot을 통한 pro-caspase 3의 caspase 3로의 전환 등을 통해 세포사멸이 발생하였음을 확연하게 확인할 수 있었으나, 반면 H1299(-pl6/-p53) cell에서는 단지 Western blot을 통한 pro-caspase 3의 활성화만을 통해 간접적으로 세포사멸을 확인 할 수 있었다. 또한 p53이 결핍된 H1299(-pl6/-p53)세포주에서의 $^{INK4A}$ 에 의한 세포사멸 유도는 p53 비의존적으로 작용한다는 사실을 확인할 수 있었다. 결론적으로 발암억제 유전자인 $^{INK4A}$ 는 CKI로서의 기능뿐 아니라, 세포사별 유도와도 밀접하게 관련되어 있으며, 이 기능은 발암 억제 유전자인 p53과는 독립적으로 작용한다는 사실을 확인하였다. 세포사멸 유도 기전연구에서 $p16^{INK4A}$ 가 세포사멸을 유도하는 기전에 대해서는 아직 명확하게 밝혀진 바는 없으며, 현재 본 연구실에서 다양한 실험을 통해 연구가 진행 중이다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권11호
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• pp.4671-4675
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• 2015

Moringa oleifera Lam. (Moringaceae) is widely consumed in tropical and subtropical regions for their valuable nutritional and medicinal characteristics. Recently, extensive research has been conducted on leaf extracts of M. oleifera to evaluate their potential cytotoxic effects. However, with the exception of antimicrobial and antioxidant activities, little information is present on the cytotoxic activity of the essential oil obtained from M. oleifera seeds. Therefore, the present investigation was designed to investigate the potential cytotoxic activity of seed essential oil obtained from M. oleifera on HeLa, HepG2, MCF-7, CACO-2 and L929 cell lines. The different cell lines were subjected to increasing oil concentrations ranging from 0.15 to 1 mg/mL for 24h, and the cytotoxicity was assessed using MTT assay. All treated cell lines showed a significant reduction in cell viability in response to the increasing oil concentration. Moreover, the reduction depended on the cell line as well as the oil concentration applied. Additionally, HeLa cells were the most affected cells followed by HepG2, MCF-7, L929 and CACO-2, where the percentages of cell toxicity recorded were 76.1, 65.1, 59.5, 57.0 and 49.7%, respectively. Furthermore, the $IC_{50}$ values obtained for MCF-7, HeLa and HepG2 cells were 226.1, 422.8 and $751.9{\mu}g/mL$, respectively. Conclusively, the present investigation provides preliminary results which suggest that seed essential oil from M. oleifera has potent cytotoxic activities against cancer cell lines.

• Asian Pacific Journal of Cancer Prevention
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• 제17권1호
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• pp.183-187
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• 2016

Rhizomes of Boesenbergia pandurata (Roxb.) Schlecht have been reported to contain active compounds with anticancer properties. This research was carried out to examine anti-proliferative and apoptotic induction against HeLa and Vero cells-line. Dried powder of B. pandurata rhizomes was extracted by a maceration method using 90% ethanol. Cytotoxic assays to determine $IC_{50}$ and anti-proliferative effects were carried out by MTT methods. Observation of apoptosis was achieved with double staining using acridine orange and ethidium bromide. The results showed that ethanolic extract of B. pandurata was more cytotoxic against HeLa cells ($IC_{50}$ of $60{\mu}g/mL$) than Vero cells ($IC_{50}$ of $125{\mu}g/mL$). The extract had higher anti-proliferative activity as well as apoptotic induction in HeLa than Vero cells. Therefore, it was concluded that the ethanolic extract of B. pandurata had anti-proliferative as well as apoptosis induction activity dependent on the cell type.

• 한국동물학회지
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• 제39권2호
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• pp.123-131
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• 1996

In this study, we examined the expression of heat shock proteins (HSPs) in fish cell line CHSE-2lnl and human HeLa cells exposed to heat shock. In fish CHSE-214 cells HSP70 was the major polvpeptide induced by an elevated temperature or an amino acid analog, while in HeLa cells HSP90 as well as HSP70 were prominently enhanced in response to these stresses. Pretreatment of actinomvcin D prior to heat shock completely inhibited the induction of fish HSP70, indicating the transcriptional regulation of fish HSP70 gene expression. In HeLa and CHSE-214 cells either recovering from heat shock or experiencing prolonged heat shock, attenuation in the HSP90 a'nd HSP70 induction occurred but both induction and repression of HSP70 synthesis appear 19 precede those of HSP90. Moreover, attenuation did not occur in the syntheses of 40 kDa and 42 kOto proteins which were only induced in CHSE-214 cells. The enhanced syntheses of these he proteins continued as long as CHSE-214 cells were Siven heat shock. These results suggest that down-regulation of HSP syntheses during prolonged heat shock may be controlled by several different. as vet undefined, mechanisms.

• Journal of Microbiology and Biotechnology
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• 제27권7호
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• pp.1359-1366
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• 2017

(E)-2-Methoxy-4-(3-(4-methoxyphenyl)prop-1-en-1-yl)phenol (MMPP), derived from butenal, is a recently synthesized Maillard reaction product. Owing to its novelty, little is known about the function of MMPP. In this study, we elucidated the effects of MMPP on apoptosis in cervical cancer by using the HeLa cervical cancer cell line, which is widely used in cancer research. We observed that MMPP was cytotoxic to HeLa cells and induced activation of caspase-3, -8, and -9, without affecting the expression of the viral oncogenes E6 and E7. In particular, the expression of the death receptors DR5 and FAS was significantly increased by MMPP treatment. There were no significant alterations of mitochondrial intrinsic factors. Taking all these results together, our findings show that MMPP primarily induces apoptosis in HeLa cervical cancer cells via the extrinsic apoptotic signaling pathway, accompanied by an enhanced expression of death receptors.

• KSBB Journal
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• 제21권3호
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• pp.171-174
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• 2006

형광을 이용한 암 진단을 위해 배양된 정상 및 암세포주에 광민감제인 5-ALA를 투여하고 세포 내 외에서 생성된 Protoporphyrin IX(PpIX)의 형광을 측정하여 5-ALA 투여의 최적농도를 조사하였다. 정상 간세포주(Chang) 및 자궁경부암 세포주(HeLa)에 5-ALA를 농도별로 투여하여 ALA에 의해 유도된 PpIX의 생성을 확인하고, MTT assay로 세포생존율을 측정하였다. 배양된 cell에 5-ALA를 투여한 후 24시간 동안 배양함으로써 생성되는 PpIX의 양은 형광의 강도로 측정하였다. HeLa 세포주에 대한 5-ALA의 최적농도는 $50{\mu}g/ml$이며, 이 때의 형광(emission) 스펙트럼은 여기 파장이 410 nm일 때 602.3 nm, 659.9 nm에서 형광 봉우리가 관찰되었다. PpIX의 형광 강도를 측정한 결과, PpIX는 정상세포에서는 낮은 농도로 축적이 되는 반면에 암세포에서 더 높은 농도로 축적되었으며, 세포 외보다는 세포 내에서 더 높은 농도로 축적됨을 알 수 있었다.