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Cell-type-specific Gene Expression Patterns in Human Carcinoma Cells followed by Irradiation  

Park Ji-Yoon (Department of Biochem & Mol. Biol, Hanyang University)
Kim Jin-Kyu (RI Radiation Research Team, Korea Atomic Energy Research Institute)
Chai Young Gyu (Department of Biochem & Mol. Biol, Hanyang University)
Publication Information
Korean Journal of Environmental Biology / v.23, no.2, 2005 , pp. 152-156 More about this Journal
Abstract
Ionizing radiation is a well- known therapy factor for human carcinoma cells. Genotoxic stress mediates cell cycle control, transcription and cellular signaling. In this work, we have used a microarray hybridization approach to characterize the cell type-specific transcriptional response of human carcinoma MCF-7 and HeLa cell line to $\gamma-radiation$, such as 4Gy 4hr. We found that exposure to $\gamma-ray$ alters by at least a $log_2$ factor of 1.0 the expression of known genes. Of the 27 genes affected by irradiation, 11 are down- regulated in MCF-7 cells and 2 genes induced by radiation,15 are repressed in HeLa cells. Many genes were involved in known damage- response pathways for cell cycling, transcription factor and cellular signaling response. However, in MCF-7 cells, we observed gene expression pattern in chromatin, apoptosis, stress, differentiation, cytokine, metabolism, ribosome and calcium. In HeLa cells, it showed clearly the expression changes in adhesion and migration, lysosome, brain, genome instability and translation. These insights reveal new therapy directions for studying the human carcinoma cell response to radiation.
Keywords
irradiation; genotoxic stress; human carcinoma cells; expression pattern; cell type-specificity;
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