• Title/Summary/Keyword: Hd1

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NMR Spectroscopy and Mass Spectrometry of 1, 2-Hexanediol Galactoside synthesized using Escherichia coli β-Galactosidase (대장균 베타-갈락토시데이즈를 이용하여 합성된 1, 2-Hexanediol Galactoside의 NMR Spectroscopy 및 Mass spectrometry)

  • Kim, Yi-Ok;Lee, Hyang-Yeol;Jung, Kyung-Hwan
    • Journal of the Korean Applied Science and Technology
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    • v.33 no.2
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    • pp.286-292
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    • 2016
  • 1, 2-Hexanediol galactoside (HD-gal) has been synthesized from 1, 2-hexanediol (HD), a cosmetic preservative, using recombinant Escherichia coli ${\beta}$-galactosidase (${\beta}$-gal) at the high lactose concentration (300 g/l). To confirm the molecular structure of synthesized HD-gal, NMR ($^1H$- and $^{13}C$-) spectroscopy and mass spectrometry of HD-gal were conducted. $^1H$ NMR spectrum of HD-gal showed multiple peaks corresponding to the galactocyl group, which is an evidence of galactocylation on HD. Downfield proton peaks at ${\delta}_H$ 4.44 ppm and multiple peaks from ${\delta}_H$3.96~3.58 ppm were indicative of galactocylation on HD. Up field proton peaks at ${\delta}_H$ 1.60~1.35 ppm and 0.92 ppm showed the presence of $CH_2$ and $CH_3$ protons of HD. $^{13}C$ NMR spectrum revealed the presence of 21 carbons suggestive of ${\alpha}$- and ${\beta}$-anomers of HD-gal. Among 12 carbon peaks from each anomers, the 3 peaks at dC 68.6, 60.9 and 13.2 ppm were assigned to be overlapped showing only 21 peaks out of total 24 peaks. The mass value (protonated HD-gal, m/z = 281.1601) from mass spectrometry analysis of HD-gal, and $^1H$ and $^{13}C$ NMR spectral data were in well agreement with the expecting structure of HD-gal. For further study, the minimum inhibitory concentrations (MICs) of HD-gal against bacteria will be investigated, and, in addition, cytotoxicity to human skin cells of HD-gal will be examined. It is expected that it will eventually be able to develop a new cosmetic preservative, which have low cytotoxicity against human skin cell and maintains antimicrobial effect.

Evaluation of Nutritional Deficit Status in Hemodialysis Patients' Based on Usual Dietary Intake, Anthropometric and Biochemical Parameters (일상식이섭취상태와 신체계측 및 혈액화학적 영양지표에 의한 혈액투석 환자의 영양결핍상태 평가)

  • Sohng Kyeong-Yae
    • Journal of Korean Academy of Fundamentals of Nursing
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    • v.3 no.1
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    • pp.68-80
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    • 1996
  • Long-term hemodialysis(HD) patients manifest various signs of protein and caloric malutrition due to poor intake of nutrients and other causes. Poor nutritional status increases the mortality and morbidity rates in HD patients. Thus, mataintnance of adequate nutritional status has been a major task in taking care of patients receiving HD. This study was to evaluate the nutritional status of HD patients and to clarify the degree of nutritional deficit based on usual dietary intake, anthropometric and biochemical indicators. Sixty HD patients comprised a HD group, while the control group consisted of 60 healthy adults whose age and sex matched those of the HD group. Nutritional status was evaluated by dietrary intake using instant nutritional scale, anthropometric measures, serum protein concentrations and the number of lymphocytes. The data were analyzed by using Chi-square test and unpaired t-test. The results are as follows. 1. Regarding usual dietary intake of HD group. 1) Estimated caloric intake was significantly lower than the recommended daily allowance(RDA) and among them, 35% were taking calories less than 85% of the RDA. 2) Estimated protein intake was significantly higher than the RDA and among them 40% were taking protein more than 115% of the RDA. 3) Estimated fat intake was lower than the RDA. 4) Vitamin A, B, $B_1,\;B_2$, C and niacin in take was lower than the RDA respectively. 5) Estimated ferrous intake was within the normal limit the RDA while estimated calcium intake was higher than the RDA. 6) Both calorie and protein intake were higher for the 10 patients who had been under continuous ambulatory peritoneal dialysis than for the patients under HD from the beginning. 2. Regarding anthropometric measures : 1) Body mass index(BMI), midarm circumference(MAC), and triceps skinfold thickness(TSF) were lower in the HD group than in the control group. 2) Among HD group, 47.1% were within the normal limit of BMI, while 86.7% were within the same limit in the control group. 3) Among HD group, 35.0% were within the normal limit of MAC, while 83.3% were within the same limit in the control group. 4) Among HD group, only 8.3% were normal, 30.3% were mild deficit status of TSF, while 50% were normal and 48.3% were mild deficit status in the control group. 3. Regarding biochemical laboratory tests 1) Albumin, transferrin concentrations and the number of lymphocytes were lower in HD group than in the control group. 2) Among HD group, 98.3% were within the normal limit of albumin concentration and all were within the same limit in the control group. 3) Among HD group, only 11.7% were within the normal limit of transferrin concentration, while 81.7% were within the same limit in the control group. 4) Among HD group, 25% were within the normal limit, while 93.3% were within the same limit in the control group. The above findings suggest that HD patients were in nutritional deficit status. Adequate diet therapy and periodical evaluation of the nutritional status in HD patients are needed. Accordingly, it turned out that anthropometric measures were very reliable parameters and easy to use to evaluate nutritional status. So nurses are encouraged to adopt anthropometric measures to examine nutritional deficit status of HD patients.

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A SEARCH FOR EXOPLANETS AROUND NORTHERN CIRCUMPOLAR STARS VI. DETECTION OF PLANETARY COMPANIONS ORBITING THE GIANTS HD 60292 AND HD 112640

  • Lee, Byeong-Cheol;Park, Myeong-Gu;Han, Inwoo;Bang, Tae-Yang;Oh, Hyeong-Il;Choi, Yeon-Ho
    • Journal of The Korean Astronomical Society
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    • v.53 no.1
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    • pp.27-34
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    • 2020
  • We report the detection of exoplanet candidates in orbits around HD 60292 and HD 112640 from a radial velocity (RV) survey. The stars exhibit RV variations with periods of 495 ±3 days and 613±6 days, respectively. These detections are part of the Search for Exoplanets around Northern Circumpolar Stars (SENS) survey using the fiber-fed Bohyunsan Observatory Echelle Spectrograph installed at the 1.8-m telescope of the Bohyunsan Optical Astronomy Observatory in Korea. The aim of the survey is to search for planetary or substellar companions. We argue that the periodic RV variations are not related to surface inhomogeneities; rather, Keplerian motions of planetary companions are the most likely interpretation. Assuming stellar masses of 1.7 ± 0.2M (HD 60292) and 1.8 ± 0.2M (HD 112640), we obtain minimum planetary companion masses of 6.5 ± 1.0MJup and 5.0 ± 1.0MJup, and periods of 495.4 ± 3.0 days and 613.2 ± 5.8 days, respectively.

A Study on Data Model Conversion Method for the Application of Autonomous Driving of Various Kinds of HD Map (다양한 정밀도로지도의 자율주행 적용을 위한 데이터 모델 변환 방안 연구)

  • Lee, Min-Hee;Jang, In-Sung;Kim, Min-Soo
    • Journal of Cadastre & Land InformatiX
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    • v.51 no.1
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    • pp.39-51
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    • 2021
  • Recently, there has been much interest in practical use of standardized HD map that can effectively define roads, lanes, junctions, road signs, and road facilities in autonomous driving. Various kinds of de jure or de facto standards such as ISO 22726-1, ISO 14296, HERE HD Live map, NDS open lane model, OpenDRIVE, and NGII HD map are currently being used. However, there are lots of differences in data modeling among these standards, it makes difficult to use them together in autonomous driving. Therefore, we propose a data model conversion method to enable an efficient use of various kinds of HD map standards in autonomous driving in this study. Specifically, we propose a conversion method between the NGII HD map model, which is easily accessible in the country, and the OpenDRIVE model, which is commonly used in the autonomous driving industry. The proposed method consists of simple conversion of NGII HD map layers into OpenDRIVE objects, new OpenDRIVE objects creation corresponding to NGII HD map layers, and linear transformation of NGII HD map layers for OpenDRIVE objects creation. Finally, we converted some test data of NGII HD map into OpenDRIVE objects, and checked the conversion results through Carla simulator. We expect that the proposed method will greatly contribute to improving the use of NGII HD map in autonomous driving.

ANALYSIS OF LONG PERIOD RADIAL VELOCITY VARIATIONS FOR HD 18438 AND HD 158996

  • Bang, Tae-Yang;Lee, Byeong-Cheol;Jeong, Gwang-hui;Han, Inwoo;Park, Myeong-Gu
    • The Bulletin of The Korean Astronomical Society
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    • v.42 no.1
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    • pp.42.4-43
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    • 2017
  • We investigate the long-period radial velocity (RV) variations for M giant HD 18438 and K giant HD 158996 using the high-resolution Bohyunsan Observatory Echelle Spectrograph at the 1.8m telescope of Bohyunsan Optical Astronomy Observatory in Korea. These two target stars are important because HD 18438 is the largest star and HD 158996 is the brightest star for exoplantary system candidate so we can understarnd how evolved stars affect planets by researching these stars. We calculated precise RV measurements of 38 and 24 spectra from November 2010 to January 2017 and June 2010 to January 2017, respectively. We dreived the RV variation period for 719.0 days of HD 18438, 775.6 days for HD 158996. We conclude that the RV variation of HD 158996 is caused by planetary companion which has the mass of 14.7 MJup, semi-major axis of 2.2 AU, and eccentricity of 0.27 assuming the stellar mass of $2.34M{\odot}$. On the other hand, the origin of RV variation of HD 18438 with period of 719.0 days is still uncertain. It might be caused by stellar chromospheric activity or planetary companion, so more observations and tests are required.

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CO TO H2 RATIO OF INTERSTELLAR MOLECULAR CLOUDS IN THE DIRECTIONS OF EARLY TYPE STARS (초기형 별 방향 성간운의 CO 와 H2 비율 계산)

  • Park, Jae-Woo;Lee, Dae-hee;Min, Kyoung-Wook
    • Journal of Astronomy and Space Sciences
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    • v.21 no.4
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    • pp.243-248
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    • 2004
  • We present measurements of interstellar CO absorption lines in the spectra of 7 early-type stars that were observed with the FUSE(Far Ultraviolet Spectroscopic Explore.) Among 54 early-type target stars in the Galactic disk and halo observed with the BBFS(Berkeley Extreme and Far-ultraviolet Spectrometer), we choose 7 program stars (HD 37903, HD 97991, HD 149881, HD 156110, HD 164794, HD 214080 and HD 219188) which have only a single velocity component in the high-resolution optical measurements, in order to avoid line blending. To analyze the CO molecule, we select the E-X (0-0) band at $1076{\AA}$, which has a large oscillate. strength and is not blended with other interstellar absorption lines. We detect the CO absorption lines in three (HD 37903, HD 164794, and HD 214080) out of seven targets, and derive CO column densities for those targets. We also estimated the CO to $H_2$ ratios toward the three stars, based on the previously estimated $H_2$ column densities.

SEARCH FOR EXOPLANETS AROUND NORTHERN CIRCUMPOLAR STARS III. LONG-PERIOD RADIAL VELOCITY VARIATIONS IN HD 18438 AND HD 158996

  • Bang, Tae-Yang;Lee, Byeong-Cheol;Jeong, Gwang-Hui;Han, Inwoo;Park, Myeong-Gu
    • Journal of The Korean Astronomical Society
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    • v.51 no.1
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    • pp.17-25
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    • 2018
  • Detecting exoplanets around giant stars sheds light on the later-stage evolution of planetary systems. We observed the M giant HD 18438 and the K giant HD 158996 as part of a Search for Exoplanets around Northern circumpolar Stars (SENS) and obtained 38 and 24 spectra from 2010 to 2017 using the high-resolution Bohyunsan Observatory Echelle Spectrograph (BOES) at the 1.8m telescope of Bohyunsan Optical Astronomy Observatory in Korea. We obtained precise RV measurements from the spectra and found long-period radial velocity (RV) variations with period 719.0 days for HD 18438 and 820.2 days for HD 158996. We checked the chromospheric activities using Ca $\text\tiny{II}$ H and $H{\alpha}$ lines, HIPPARCOS photometry and line bisectors to identify the origin of the observed RV variations. In the case of HD 18438, we conclude that the observed RV variations with period 719.0 days are likely to be caused by the pulsations because the periods of HIPPARCOS photometric and $H{\alpha}$ EW variations for HD 18438 are similar to that of RV variations in Lomb-Scargle periodogram, and there are no correlations between bisectors and RV measurements. In the case of HD 158996, on the other hand, we did not find any similarity in the respective periodograms nor any correlation between RV variations and line bisector variations. In addition, the probability that the real rotational period can be as longer than the RV period for HD 158996 is only about 4.3%. Thus we conclude that observed RV variations with a period of 820.2 days of HD 158996 are caused by a planetary companion, which has the minimum mass of 14.0 $M_{Jup}$, the semi-major axis of 2.1 AU, and eccentricity of 0.13 assuming the stellar mass of $1.8 M_{\odot}$. HD 158996 is so far one of the brightest and largest stars to harbor an exoplanet candidate.

Cloning and Expression of the Bacillus thruingiensis var. kurstaki HD-1 Crystal Protein gene in Eschelichia coli

  • Sang Hyn Kim;You
    • Journal of Sericultural and Entomological Science
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    • v.35 no.2
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    • pp.129-133
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    • 1993
  • The 44Md plasmid of Bacillus thruingiensis var. kurstaki HD-1(B. t k HD-1) was partially digested with Sau3AI and the fragments were cloned into E. coli HB101 on vector pBR322. Of 2, 950 clones with a recombinant pBR322, only one clone KC1 was determined to have the gene for crystal toxic proteins from the 44Md plasmid of B. t k HD-1 at the BamHI site of pBR322. The recombinant pBR322 was named pKC1 and its molecular size was 12kb. The KC1 produced a protein which was toxic to the silkworm and antigenically similar to the crystal toxic protein of B. t k HD-1. Also, electrophoretic mobility of the KC1 protein was apparently the same as that of the crystal toxic protein of B. t k HD-1.

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Gibberellins Production and Identification of Endophytic Fungi Isolated from Aquatic Plant in Fresh Water (담수에 자생하는 수생식물에서 분리된 내생균류의 지베렐린 생산과 동정)

  • You, Young-Hyun;Kang, Sang-Mo;Choi, Yu-Mi;Lee, Myung-Chul;Kim, Jong-Guk
    • The Korean Journal of Mycology
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    • v.43 no.1
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    • pp.71-76
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    • 2015
  • Aquatic plant Hydrocharis dubia (Blume) Backer was collected from the Dalsung wetland in Daegu. Sixteen endophytic fungi with different colony morphologies were isolated from the roots of aquatic plants. Waito-c rice (WR) seedlings were treated with fungal culture filtrates (FCF) for screening plant growth-promoting activity. In the results, HD1008 strain isolated from aquatic plant showed highest plant growth-promoting activity. The FCF of HD1008 strain was analyzed using gas chromatography mass spectrometry (GC/MS) with selected ion monitoring (SIM). Analysis of the FCF of HD1008 strain found that it contained gibberellins (GA) ($GA_1$, 1.2 ng/100 mL; $GA_4$, 5 ng/100 mL). Phylogenetic tree of HD1008 strain was constructed by partial internal transcribed spacer (ITS) region and partial beta-tubulin gene sequences. Therefore, we describe HD1008 strain as a new gibberellin-producing Penicillium trzebinskii based on morphological and molecular characteristics.

A study on the interference on FM Radio channel from HD Radio signal (HD Radio에 의한 FM 방송의 간섭 연구)

  • Lee, Sang-Woon
    • Journal of Satellite, Information and Communications
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    • v.7 no.1
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    • pp.122-127
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    • 2012
  • The purpose of this study is to know about the interference on FM Radio channel from HD Radio signal. In Korea, a data service channel, named DARC is operated by a major FM radio broadcaster which has national wide service area. Therefore, an analysis of the interference on FM radio channel with DARC data channel from HD Radio, most recently developed digital radio system. Technical parameters of FM radio with DARC data channel and HD Radio system are considered for this simulation. As results some interference generated because of HD Radio signal according to its signal allocation and service mode of HD Radio.