• Ocean and Polar Research
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• v.28 no.3
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• pp.287-290
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• 2006

Eye growth and lens diameter of brown croaker Miichthys miiuy were positively allometric between hatching and 180 days post-hatch (d.p.h.). Eye growth in relation to head length and head height was nearly isometric. Eyes were formed completely at 14 d.p.h. At this age, the eye has a crystalline lens, an optic nerve fiber layer, a ganglion cell layer, an inner plexiform layer, an inner nuclear layer, an outer plexiform layer, an outer nuclear layer, an outer limiting membrane, and a pigment epithelium. The essential demands that must be met by the retina in this species pertain to light sensitivity and spatial resolution.

• Environmental Analysis Health and Toxicology
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• v.17 no.1
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• pp.1-6
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• 2002

Effects on nonylphenol (NP) treatment on early development of fertilized eggs and survival of larvae in olive flounder, Paralichthys olivaceus were investigated. Fertilized eggs and hatched larvae were exposed to aqueous solutions of NP at nominal concentrations of 50, 100, 150 and 200 $\mu\textrm{g}$/L. In the control I and II (methanol-carrier control) groups, the hatchability of fertilized eggs were 90.7$\pm$7.02 and 90.0$\pm$5.29％ (P <0.05), respectively. However, treatments of NP concentrations at 50, 100, 150 and 200 ug/L were reduced to 78.7$\pm$4.16, 46.0$\pm$9.17, 48.0$\pm$3.46 and 33.3$\pm$11.02% (P<0.05), respectively. The time to hatching of fertilized eggs was delayed in high-dose NP treatment groups rather than control groups. Fertilized eggs of NP immersed group could not hatch normally, and also vertebra of the larvae observed as abnormal shape. Cumulative mortality of hatched larvae after 26hr NP treatment was 38.3, 78.3, 88.3 and 100% in NP 50, 100, 150 and 200 ug/L treatment groups, respectively. Whereas, control I and II were 11.7, 16.7% (P<0.05). Hatched larvae showed most death at the embryonic development stage with abnormalities. These results suggest that NP treatment in fertilized eggs and larvae was inhibited as normally development for fertilized egg and growing of larvae.

• Korean Journal of Ecology and Environment
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• v.48 no.4
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• pp.280-286
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• 2015

Life cycles of Sweltsa illiesi and S. lepnevae were determined from a headwater stream in Mt. Jumbong. Identification of nymphs was done by using DNA barcoding. Nymphs begin to have species specific color pattern on their head several months prior to adult emergence and differ in the form of the third antennal segment. Two species appear to have similar semivoltine life cycles in this stream, beginning to hatch from June and finishing adult emergence about 701 days later. The combined annual mean biomass in ash free dry mass (AFDM) was estimated as $96mg\;AFDM\;m^{-2}$. The combined annual secondary production were 373 mg and $297mg\;AFDM\;m^{-2}\;yr^{-1}$, calculated by using size frequency method and increment summation method respectively.

• Journal of Embryo Transfer
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• v.23 no.1
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• pp.1-4
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• 2008

This study was performed to investigate the effect of laser-assisted hole in the zona pellucida (ZP) of frozen-thawed ICR mouse embryos on the process of hatching that is critical for expanded blastocysts to implant into endometrium, Vitrification medium, composed of ethylene glycol and sucrose supplemented with 7.5% (w/v) PVP, was used to freeze $2{\sim}4$ cell stage embryos recovered from oviducts of superovulated and mated female mice before storing them in $LN_2$. Right after thawing them, a laser beam was shot to make a hole in ZP followed by culturing in KSOM for $96{\sim}120\;hr$ and examining development to blastocyst and hatching every 12 hr. Laser-treated embryos showed significantly higher hatching rate compared to control (92.9% vs. 22.1%, p<0.05). From around Day 4, blastocysts developed from laser-treated embryos started hatching while the blastocysts of control group failed to hatch showing a lot of shrinkage. This study shows that a laser-assisted hole in ZP improves the hatching rate of blastocysts developed from frozen-thawed, in vitro cultured ICR mouse embryos.

• Journal of Welding and Joining
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• v.28 no.4
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• pp.33-40
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• 2010

The purpose of this study is to establish a predictive equation of transverse residual stress at the thick FCA butt weldment of large container vessel. The variables used were restraint degree, yield strength of base material, thickness of weldment and welding heat input. Restraint degree at the thick weldment of container ship having the various welding sequence was calculated using FEA. From the result, the H-type specimen was designed to reproduce the level of restraint degree at the actual weldment of containership. Based on the results, the predictive equations of the mean value and the distribution of transverse residual stress at each location of the weldment were established using dimensional analysis and multiple-regression method. The predictive equations were verified by comparing with those measured by XRD in the actual weldment of the ship.

• Development and Reproduction
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• v.19 no.4
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• pp.189-196
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• 2015

This study was examined the ovogenesis of Ussurian bullhead, Leiocassis ussuriensis and the morphological development of its larvae and juveniles and to use the results as basic information for the preservation of species and resource enhancement. For artificial egg collection, human chorionic gonadotropin (HCG) was injected at a rate of 10 IU per gram of fish weight. During breeding period, water temperature maintained at $24.5{\sim}26.5^{\circ}C$ (mean $25.0{\pm}0.05^{\circ}C$). The process of ovogenesis reached the two-cell stage in 50 minutes after fertilization. In 73 hours of fertilization the movement of the embryoid body became active state and the larvae began to hatch from the tail through the oolemma. Length of prelarvae were 6.33~6.50 mm long (mean $6.40{\pm}0.06mm$) just after hatching having yolk with their mouth not opened. After thirty eight days of hatching, juveniles were 30.6~32.5 mm long (mean $31.5{\pm}0.65mm$). The color was dark yellowish brown throughout the entire body, and the number of caudal fin rays developed to thirty six perfectly.

• Journal of Animal Science and Technology
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• v.57 no.10
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• pp.30.1-30.4
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• 2015

The present study was aimed to study the light and electron microscopic studies of thymic Hassall's corpuscles was done in various age groups of Nandanam Chicken ranging from day-old to forty weeks. Hassall's corpuscles are special, unique structures present in thymic medulla and also in the cortex of all the age groups of Nandanam chicken (from hatch to forty weeks) in the present study. Size of the Hassall's corpuscles in the medulla is larger than the ones present in the cortical region of thymus. The Hassall's corpuscles are made up of structureless eosinophilic mass surrounded by concentrically arranged reticuloepithelial cells. Under electron microscope, the Hassall's corpuscles were composed of reticuloepithelial cells interconnected by many desmosomes. The epithelial cells had abundance of cytoplasmic fibrils and desmosomes with few mitochondria and ribosomes. The nucleus was oval or round which was slightly indented. The centre of the Hassall's corpuscles was appeared either solid or cystic. The cystic corpuscles had cell debris within the cyst lumen.

• Fisheries and Aquatic Sciences
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• v.21 no.2
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• pp.4.1-4.5
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• 2018

Insulin-like growth factors (IGFs), along with IGF-binding protein and IGF receptor, are well-known regulators in the growth and survival of vertebrates. In this study, we investigated the involvement of IGFs and protein variation during embryonic development of the olive flounder (Paralichthys olivaceus). Morphological stages were divided into six main developments as blastula, gastrula, cephalization, cranial regionalization, tail lift, and hatch. During embryonic development, protein variation was investigated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electrospray ionization quadrupole time-of-flight mass spectrometry/mass spectrometry. In addition, the mechanism of signaling of IGF-I receptor was examined using immuno-blot analysis. We found marked changes in protein expression at four stages of embryonic development and identified proteins as belonging to the vitellogenin 2 family. As development progresses, expression of IGF-II, phosphotyrosine, and phospho-Akt increased, while expression of growth factor receptor-bound protein 2 (GRB2) and one of guanine-nucleotide-binding proteins (Ras) decreased. These results provide basic information on the IGF system in the embryonic development of the olive flounder.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.56 no.2
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• pp.126-137
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• 2020

This study collected and analyzed the fishing process of existing fishing boat and newly built fishing boat by using the video observation methods to understand the improvement of fishing operation efficiency and safety according to the scale change of coastal composite fishing boat. The fishing operation efficiency was calculated by analyzing the frequency of movement, the movement distance and the moving time per basket used in the fishing process to derive the improvement of the newly built fishing boat compared to the existing fishing boat. It was confirmed that the mean frequency of movements decreased to 13.9%, the average moving time decreased to 21.8%, the mean movement distance increased to 20.5% and the movement through the top of gunwale did not occur. Movement of frequency, increased and time according to the fishing operation were directly affected by the width of side passages and the presence or absence of walking obstruction such as bulwark stay, hatch coaming and fishing gears on deck. The results of this study are expected to be used as basic data for redesigning into a safe and efficient coastal composite fishing boat in the future.

• Journal of the Korean Applied Science and Technology
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• v.21 no.4
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• pp.300-305
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• 2004

High-solid coatings were prepared by blending of previosly synthesized acrylic resins and hexamethylene diisocyanate-trimer and curing it at room temperature. The characterization of the films of the prepared coatings was performed. The impact resistance, cross-hatch adhesion, $60^{\circ}$specular gloss, and heat resistance of the films proved to be good, and the pencil hardness and drying time proved to be slightly poor. Especially, there was a remarkable improvement in the heat resistance. This improvement may stem from the regular arrangement of ethyl groups introduced into the acrylic resin. As a result of Rigid-body pendulum visco-elasticity measurement, dynamic $T_g$ values of cured films increased with dynamic $T_g$ values.