• Journal of Chest Surgery
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• v.23 no.5
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• pp.844-853
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• 1990

We have performed 27 cases of orthotopic homologous cardiac transplantation using Korean mongrel dogs and one case of sham operation for the evaluation of harmful effect of cardiopulmonary bypass itself on the dog from April, 1989 to June, 1990. Our previous reports have already demonstrated basal hemodynamic and hematologic data on the canine homologous heart transplantation and the fundamental principles of transplantation of the heart. The mean body weight of recipients was 13.2$\pm$1.2kg with a rage of 11 ~ 15kg, and the hemodynamic and hematologic pictures were almost same as the result of previous reports from our hospital, except marked decrease in postoperative platelet count[from 3.18 $\pm$0.80x106/mm3 to 1.41$\pm$0 37x 106/mm3]. Mean survival time was 24.82$\pm$49.40 hours with the longest survival of 264 hours. Donor cardiectomy included coronary vasodilatation with diltiazem, potassium arrest, and the rapid cooling of the heart suspending in the specially designed ice-bath. Median sternotomy provided excellent exposure of the surgical field. 6 \ulcorner0 prolene suture was used for the anastomosis of both atrial cuffs and the great arteries, and we found the fact that stenosis, bleeding, thrombus formation around the anastomotic site could be decreased with the use of everted horizontal mattress suture techniques. Immunosuppression was done with a combination of lower dose Cyclosporin-A, Azathioprine, methyl-prednisolone, but our cases still showed too short survival to worry about graft rejection. Still poor was our quality control of experimental animal, we had much difficulties in postmortem evaluation of the dogs. Low cardiac output due to biventricular failure, intractable supraventricular or ventricular tachyarrhythmia, postoperative massive bleeding, sepsis were most frequent findings that could be thought as a cause of death. A few cases showed subendocardial patch hemorrhage in both ventricular cavity or atrial septum at autopsy, suggesting acute subendocardial infarction. Although our team overcome most of the technical problems of orthotopic heart transplantation, we should pile up further knowledges about donor heart preservation, quality control of animal, infection, rejection, the effect of the cardiopulmonary bypass to improve the results.

• Food Science of Animal Resources
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• v.21 no.4
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• pp.383-388
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• 2001

This study was conducted to analyzed the chemical compositions of Korean propolis. In proximate analysis, the moisture, crude protein. crude fat. ash and carbohydrate content of Yecheon and Youngwol propolis were 3.25 and 3.97%, 9.82 anti 7.04%, 48.25 and 50.7%, 3.91 and 5.89%, 34.77 and 32.4%, respectively. The highest level of P, Ca and Cu were revealed at Youngwol propolis as much as 184.15, 128.32 and 1.77ppm. As a harmful heavy meta1s, the Cr. AS, Cd, find Pb level of Australian propolis were revealed 2.2888, 0.9534, 0.2611 find 2.5595ppm. However, Korean propolis was not detected the Cd. The benzoic acid content of Yecheon and Youngwol propolis were 1,130.6 ppm and 1,049.1 ppm. And than Chinese product revealed the highest levee of benzoic acrid content. The linoleic and linolenic acid content of Yecheon and Youngwol propolis were 47.6:70.0 and 37.3:47.0mg/100g, respectively.

• Korean Journal of Veterinary Research
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• v.50 no.3
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• pp.197-203
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• 2010

Pesticides are useful to eliminate harmful insects and grow crops however, misuse and abuse of pesticides may cause a death of wild birds, livestock, and companion animals. We analyzed residual pesticides in the ingesta and tissues from the dead wild birds, livestock, and companion animals which were suspected pesticide poisoning based on the diagnosis of the Animal Disease Diagnostic Center of National Veterinary Research and Quarantine Service (NVRQS). The samples were primarily brought to NVRQS from local communities and veterinary diagnostic laboratories. The 231 suspicious samples of pesticide poisoning based on the necropsy were analyzed by GC/NPD, GC/FPD, GC/ECD, or GC/MSD in 2008 and 2009. Pesticides were identified from the 55 samples of total 143 samples analyzed in 2008 and from the 34 samples of total 88 samples analyzed in 2009. The pesticide identification rates in 2008 were higher than the rates in 2009. It may have relevance to the increased samples with the outbreak of avian influenza in 2008 in Korea. The concentrations of pesticides found in the dead animals varied and exceeded the respective $LD_{50}$ of pesticides in many cases. Monochrotophos, phosphamidon, and methomyl were the most common pesticides found and those pesticides are high rank sales in Korea. The cause of pesticide poisoning in animal is assuming a misuse and an abuse of commonly used pesticides in Korea.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.12
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• pp.1895-1900
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• 2007

An experiment was carried out to investigate the effects of different chilling temperature on duck breast and leg meat quality. Duck carcasses were chilled for 30 minutes in water at either $0^{\circ}C$, $10^{\circ}C$ or $20^{\circ}C$ within 20 minutes of post mortem with 6 carcasses per group. Results showed no significant effects of chilling temperature on ultimate pH, protein solubility, sarcomere length and shear force value for duck breast or leg meat (p>0.05). Leg meat had higher ultimate pH, redness and shear force value, lower cooking loss, lightness, yellowness and protein solubility values than breast meat. The interaction of meat type and chilling temperature on cooking loss was significant (p<0.05). The effect of chilling temperature on cooking loss was more severe in leg meat than breast meat and $20^{\circ}C$ chilling resulted in significantly higher cooking losses than the other chilling temperatures. Results of this experiment revealed that duck carcass can be chilled at $10^{\circ}C$ without any harmful effect on meat quality including toughness of meat.

• Journal of Society of Preventive Korean Medicine
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• v.9 no.1
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• pp.119-133
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• 2005

Traditional herbal medicine is widely used among the Korean people, and other eastern Asian countries employ similar therapies as well. In recent years, due to increasing interest in herbal medicines, many researches have been made on the toxicity and side effects of herbal medications. Through private and public media, there have been many opinions suggesting taking herbal medicines is very harmful, especially on the liver and kidney functions. This assertion has been mainly presented by the doctors that practice western medicine, But this assertion is never based on adequate knowledge of herbal medicine. This study aims to provide the evidences that taking herbal medicines is safe on the renal functions. Four frequently used herbal medications(Sipjeondaebotang, Bojungigitang, Ojeoksan, and Yukmijihwangtang) were used to test the toxicity of herbal medicine oh the lab animal model(SD-Rat). There is no significant difference in body weight and kidney weight after herbal medication for 1 month. In all experimental groups, no abnormal findings were observed in histological study, and lab renal function index(BUN, creatinine, uric acid). These results say that four herbal multi-used-medicines, when medicated, is safe from the renal toxicity in lab animal model.

• Journal of Animal Environmental Science
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• v.15 no.1
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• pp.59-68
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• 2009

This study investigated the evaluation of maturity, stability, nutrient and heavy metal from rotating drum composter of food waste amended with poultry manure composting inoculated with effective microorganisms(EM). Composting were performed for the first, drying reactor($15m^3$) 3 hours and the second, composting reactor($30m^3$) 24 hours, and parameters monitored this period included moisture content, NaCl, pH, electrical conductivity(EC), C/N ratio, organic matter(OM), nutrient content and heavy metal. Changes in compost temperature during composting were maintained constantly in the range of $60{\sim}80^{\circ}C$ using firewood boiler(450 MJ/h). We examined physicochemical parameters and heavy metals in order to assess their effectiveness as stability and maturity, nutrient and harmful indicators such as seed germination rate<60%, potassium 1>%, dm and NaCl>1%, dm at the end of the final compost. The finished compost obtained after decomposition phase at the end of the 2nd composter could not be utilized for land improvement or reclamation.

• Journal of Animal Science and Technology
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• v.63 no.4
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• pp.799-814
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• 2021

Information is lacking about the effect of date of a fall cut of alfalfa (Medicago sativa L.) on dry matter yield (DMY), forage nutritive value, and stands persistence. The objective of this study was to determine the effect of timing of a fall cut on DMY, forage nutritive value and stand persistence of three alfalfa varieties: low-lignin Hi-Gest 360, Roundup Ready Tonica, and conventional Gunner in Northeastern Kansas in the United States. The field study was carried out by splitting plot in randomized complete block design with four replications. The harvesting data of different maturity stages were collected in each year from 2015 to 2018. Three cuts were harvested based on the stage of maturity, and the last (fourth) cut was done on September 15, September 30, October 15, and October 30 of each year. The persistence of the alfalfa stands was determined each fall after the last cut, and each spring after the first cut, by counting the number of live plants in a randomly placed quadrat in each plot. Alfalfa cut on September 15 and September 30 had a higher stand persistence compared to alfalfa cut on October 15 and October 30. The DMY of the first cut in 2016 was significantly higher in roundup ready than the low-lignin alfalfa variety. In the second cut, DMY was significantly higher in conventional alfalfa than the roundup ready. There were no significant differences in DMY between alfalfa varieties in the rest of seasonal cuttings in 2016 and 2017 and annual total yield in both years. In general, low lignin alfalfa variety had higher crude protein and relative feed value and lower acid detergent fiber and neutral detergent fiber contents than those in roundup ready and conventional alfalfa varieties. On average, nutritive value of alfalfa was generally affected by last cutting dates in 2017. Based on 3-year data the last cutting of alfalfa in the fall could be done by September 30-October 15 without harmful effect on DMY.

• ALGAE
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• v.36 no.4
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• pp.315-326
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• 2021

Ion channels are membrane protein complexes mediating passive ion flux across the cell membranes. Every organism has a certain set of ion channels that define its physiology. Dinoflagellates are ecologically important microorganisms characterized by effective physiological adaptability, which backs up their massive proliferations that often result in harmful blooms (red tides). In this study, we used a bioinformatics approach to identify homologs of known ion channels that belong to 36 ion channel families. We demonstrated that the versatility of the dinoflagellate physiology is underpinned by a high diversity of ion channels including homologs of animal and plant proteins, as well as channels unique to protists. The analysis of 27 transcriptomes allowed reconstructing a consensus ion channel repertoire (channelome) of dinoflagellates including the members of 31 ion channel families: inwardly-rectifying potassium channels, two-pore domain potassium channels, voltage-gated potassium channels (K_v), tandem K_v, cyclic nucleotide-binding domain-containing channels (CNBD), tandem CNBD, eukaryotic ionotropic glutamate receptors, large-conductance calcium-activated potassium channels, intermediate/small-conductance calcium-activated potassium channels, eukaryotic single-domain voltage-gated cation channels, transient receptor potential channels, two-pore domain calcium channels, four-domain voltage-gated cation channels, cation and anion Cys-loop receptors, small-conductivity mechanosensitive channels, large-conductivity mechanosensitive channels, voltage-gated proton channels, inositole-1,4,5-trisphosphate receptors, slow anion channels, aluminum-activated malate transporters and quick anion channels, mitochondrial calcium uniporters, voltage-dependent anion channels, vesicular chloride channels, ionotropic purinergic receptors, animal volage-insensitive cation channels, channelrhodopsins, bestrophins, voltage-gated chloride channels H⁺/Cl^- exchangers, plant calcium-permeable mechanosensitive channels, and trimeric intracellular cation channels. Overall, dinoflagellates represent cells able to respond to physical and chemical stimuli utilizing a wide range of G-protein coupled receptors- and Ca²⁺-dependent signaling pathways. The applied approach not only shed light on the ion channel set in dinoflagellates, but also provided the information on possible molecular mechanisms underlying vital cellular processes dependent on the ion transport.

• Journal of the Korean Applied Science and Technology
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• v.35 no.4
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• pp.1230-1242
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• 2018

In the present work, we reviewed feed mycotoxin-related research and provide potential strategies to overcome feed mycotoxin issues. Cereal grains and cereal byproducts are most easily contaminated by fungus. Fungi in feed ingredients produce secondary metabolites such as aflatoxin, deoxynivalenol, and zearalenone, which are commonly found in feed ingredients. These mycotoxins in animal feeds and ingredients are regulated in many countries. Dietary mycotoxins have detrimental effects on immune systems and growth performance in pigs. A major harmful effect of dietary mycotoxin is reduced feed intake, resulting in deficient energy and nutrient intake and eventually depressed growth of pigs. The reduced energy and nutrient intake may be overcome possibly by increased energy and nutrient concentrations. Dietary supplementation of some mycotoxin binders may reduce the detrimental effects of mycotoxins. However, the effects of mycotoxin binders especially on deoxynivalenol and zearalenone have been reported to be variable depending on classes and concentration of mycotoxin, environmental condition, and type of mycotoxin binders.

• Journal of Animal Science and Technology
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• v.65 no.3
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• pp.519-534
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• 2023

Rumination in cattle is closely related to their health, which makes the automatic monitoring of rumination an important part of smart pasture operations. However, manual monitoring of cattle rumination is laborious and wearable sensors are often harmful to animals. Thus, we propose a computer vision-based method to automatically identify multi-object cattle rumination, and to calculate the rumination time and number of chews for each cow. The heads of the cattle in the video were initially tracked with a multi-object tracking algorithm, which combined the You Only Look Once (YOLO) algorithm with the kernelized correlation filter (KCF). Images of the head of each cow were saved at a fixed size, and numbered. Then, a rumination recognition algorithm was constructed with parameters obtained using the frame difference method, and rumination time and number of chews were calculated. The rumination recognition algorithm was used to analyze the head image of each cow to automatically detect multi-object cattle rumination. To verify the feasibility of this method, the algorithm was tested on multi-object cattle rumination videos, and the results were compared with the results produced by human observation. The experimental results showed that the average error in rumination time was 5.902% and the average error in the number of chews was 8.126%. The rumination identification and calculation of rumination information only need to be performed by computers automatically with no manual intervention. It could provide a new contactless rumination identification method for multi-cattle, which provided technical support for smart pasture.