• Clinical and Experimental Pediatrics
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• 제53권5호
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• pp.616-622
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• 2010

Enterovirus 71 (EV71) has been recognized as a frequent cause of epidemics of hand-foot-and-mouth disease (HFMD) associated with severe neurological symptoms. In the spring of 2009, HFMD was epidemic in Korea. Severe cases with complication, including death, have been reported and it has become a public health issue. Most symptomatic EV71 infections commonly result in HFMD or herpangina. These clinical manifestations can be associated with neurologic syndromes frequently. Neurologic syndromes observed in EV71 include meningitis, meningoencephalomyelitis, poliomyelitis-like paralytic disease, Guillain-Barr$\acute{e}$ syndrome, transverse myelitis, cerebellar ataxia, opsoclonus-myoclonus syndrome, benign intracranial hypertension, and brainstem encephalitis. Examinations for EV 71 were performed from the stools, respiratory secretion or CSF of the children by realtime PCR. Gene analysis showed that most of them were caused by EV71 subgenotype C4a which was prevalent in China, 2008. Public health measures including personal and environmental hygiene, must to target daycare centers, kindergartens, and schools where highly susceptible children congregate. To prevent the spread of infection, preschools where transmission persists for more than 2 incubation periods, have been recommended for closure, and trigger criteria for voluntary closure was instituted. During closure, operators are to thoroughly clean the centers before they are allowed to reopen. In addition, parents are advised to ensure that their children adopt a high standard of personal hygiene and to keep the infected child at home until full recovery. Because the outbreaks occur in a cyclical pattern, surveillance system to predict next outbreaks and adequate public health measures to control need to be planned for future. Control of EV71 epidemics through surveillance and public health intervention needs to be maintained in Korea. Future research should focus on understanding of EV71 virulence, identification of the receptor(s) for EV71, development of antiviral agents and development of vaccine.

• 한국환경보건학회지
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• 제46권6호
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• pp.710-718
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• 2020

Objectives: We analyzed water in waterscape facilities to investigate contamination levels of water-borne pathogens and four test items (pH, turbidity, residual chlorine, and Escherichia coli) at facilities including play fountains, splash parks, and artificial streams from June to October in Suwon City and in the whole of Gyeonggi-do. Methods: A total of 62 waterscape facility samples were collected from 36 sites and tested for pathogenic Escherichia coli and water-borne viruses that cause hand-foot-and-mouth disease, eye disease, and acute enteritis. Results: None of the water-borne pathogens were detected in waterscape facility samples collected from across Gyeonggi-do that were for pre-inspection for facility management. However, the results of samples from Suwon collected in hot weather and during the school vacation period showed five total inconsistencies in turbidity (four cases) and Escherichia coli (one case). Three out of the four inconsistent samples in turbidity were from the same facility which operated a sand filtration system due to its locational factors close to mountains. Conclusion: We suggest that the waterscape facilities in Gyeonggi-do are managed properly in the respect of microbial contamination and water quality.

• Biomolecules & Therapeutics
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• 제22권1호
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• pp.41-46
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• 2014

Enterovirus 71 (EV71) is the predominant cause of hand, foot and mouth disease (HFMD). The antiviral activity of hederasaponin B from Hedera helix against EV71 subgenotypes C3 and C4a was evaluated in vero cells. In the current study, the antiviral activity of hederasaponin B against EV71 C3 and C4a was determined by cytopathic effect (CPE) reduction method and western blot assay. Our results demonstrated that hederasaponin B and 30% ethanol extract of Hedera helix containing hederasaponin B showed significant antiviral activity against EV71 subgenotypes C3 and C4a by reducing the formation of a visible CPE. Hederasaponin B also inhibited the viral VP2 protein expression, suggesting the inhibition of viral capsid protein synthesis.These results suggest that hederasaponin B and Hedera helix extract containing hederasaponin B can be novel drug candidates with broad-spectrum antiviral activity against various subgenotypes of EV71.

• 한국산업융합학회 논문집
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• 제24권6_2호
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• pp.707-713
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• 2021

Symptoms of foot-and-mouth disease include fever and drooling a lot around the hoof, blisters in the mouth, poor appetite, blisters around the hoof, and blisters around the hoof. Research is underway on smart barns that remotely manage these symptoms through cameras. Visible light cameras can measure the condition of livestock such as blisters, but cannot measure body temperature. On the other hand, infrared thermal imaging cameras can measure body temperature, but it is difficult to measure the condition of livestock. In this paper, we propose an object detection system using deep learning-based livestock detection using visible and infrared thermal imaging composite camera modules for preemptive response

• Clinical and Experimental Vaccine Research
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• 제12권4호
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• pp.291-297
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• 2023

Enterovirus infections induce infectious diseases in young children, such as hand, foot, and mouth disease which is characterized by highly contagious rashes or blisters around the hands, feet, buttocks, and mouth. This predominantly arises from enterovirus A71 or coxsackievirus A16 infections and in severe cases, they can lead to encephalitis, paralysis, pulmonary edema, or even fatality, representing a global health threat. Due to the absence of effective therapeutic strategies for these infections, various experimental animal models are being investigated for the development of vaccines. During the early stages of research on enterovirus infections, non-human primate infections exhibited symptoms like those in humans, leading to their utilization as model animals. However, due to economic and ethical considerations, their current usage is limited. While enterovirus infections do not readily occur in mice, an infection model with mouse-adapted strain in neonatal mice has been employed. Cellular receptors have been identified in human cells, and genetically modified mice expressing these receptors have been used. Most recently, the utilization of Mongolian gerbil model is actively being considered and should be pursued for further animal model development. So, herein, we provide a summarized overview of the current portfolio of available enterovirus infection models, emphasizing their respective advantages and limitations.

• Biomolecules & Therapeutics
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• 제31권3호
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• pp.350-358
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• 2023

Hand-foot-and-mouth disease (HFMD) is a viral infectious disease that occurs in children under 5 years of age. Its main causes are coxsackievirus (CV) and enterovirus (EV). Since there are no efficient therapeutics for HFMD, vaccines are effective in preventing the disease. To develop broad coverage against CV and EV, the development of a bivalent vaccine form is needed. The Mongolian gerbil is an efficient and suitable animal model of EV71 C4a and CVA16 infection used to investigate vaccine efficacy following direct immunization. In this study, Mongolian gerbils were immunized with a bivalent inactivated EV71 C4a and inactivated CVA16 vaccine to test their effectiveness against viral infection. Bivalent vaccine immunization resulted in increased Ag-specific IgG antibody production; specifically, EV71 C4a-specific IgG was increased with medium and high doses and CVA16-specific IgG was increased with all doses of immunization. When gene expression of T cell-biased cytokines was analysed, Th1, Th2, and Th17 responses were found to be highly activated in the high-dose immunization group. Moreover, bivalent vaccine immunization mitigated paralytic signs and increased the survival rate following lethal viral challenges. When the viral RNA content was determined from various organs, all three doses of bivalent vaccine immunization were found to significantly decrease viral amplification. Upon histologic examination, EV71 C4a and CVA16 induced tissue damage to the heart and muscle. However, bivalent vaccine immunization alleviated this in a dose-dependent manner. These results suggest that the bivalent inactivated EV71 C4a/CVA16 vaccine could be a safe and effective candidate HFMD vaccine.

• Clinical and Experimental Vaccine Research
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• 제13권2호
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• pp.132-145
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• 2024

Purpose: Enterovirus 71, a pathogen that causes hand-foot and mouth disease (HFMD) is currently regarded as an increasing neurotropic virus in Asia and can cause severe complications in pediatric patients with blister-like sores or rashes on the hand, feet, and mouth. Notwithstanding the significant burden of the disease, no authorized vaccine is available. Previously identified attenuated and inactivated vaccines are worthless over time owing to changes in the viral genome. Materials and Methods: A novel vaccine construct using B-cell derived T-cell epitopes from the virulent polyprotein found the induction of possible immune response. In order to boost the immune system, a beta-defensin 1 preproprotein adjuvant with EAAAK linker was added at the N-terminal end of the vaccine sequence. Results: The immunogenicity of the designed, refined, and verified prospective three-dimensional-structure of the multi-epitope vaccine was found to be quite high, exhibiting non-allergenic and antigenic properties. The vaccine candidates bound to toll-like receptor 3 in a molecular docking analysis, and the efficacy of the potential vaccine to generate a strong immune response was assessed through in silico immunological simulation. Conclusion: Computational analysis has shown that the proposed multi-epitope vaccine is possibly safe for use in humans and can elicit an immune response.

• Journal of Yeungnam Medical Science
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• 제30권1호
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• pp.58-61
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• 2013

Enterovirus commonly causes neurologic diseases (aseptic meningitis, encephalitis, etc.), hand-foot-mouth disease, herpangina, and acute hemorrhagic conjunctivitis. However, it rarely causes pneumonia in immunocompetent adults. In Korea, no case has been reported about pneumonia caused by enterovirus in healthy adults. We can cite the case of a 20-year-old woman who presented severe community-acquired pneumonia caused by enterovirus. The diagnosis was based on reverse transcriptase polymerase chain reaction (RT-PCR) of a respiratory specimen.

• 한국산학기술학회논문지
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• 제20권10호
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• pp.425-432
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• 2019

본 연구는 한국언론진흥재단 BIGKinds를 통해 수집한 구제역 등의 축산 질병 관련 보도가 소비자의 육류소비에 미치는 영향요인들을 알아보기 위하여 생존 분석을 이용하여 분석하였다. 소비자 구매자료는 농촌진흥청의 농식품 소비자 패널조사 자료를 이용하였으며, 생존시간에 대한 분석결과 국내산 소고기와 돼지고기, 수입산 소고기와 돼지고기 모두에서 FMD(구제역) 보도가 유의한 영향을 미치며, FMD(구제역) 관련 보도로 인해 첫 구매율이 감소한 것으로 나타났다. 시간 의존 Cox 비례 해저드 모형을 이용하여 세부적으로 분석한 결과 모든 모형은 5% 유의수준에서 유의한 것으로 분석되었다. 각 모형에서 공통적으로 유의한 것으로 분석된 것은 FMD 관련 보도 건수로 나타났다. 2010년 국내산과 수입산 돼지고기의 Haz. Ratio는 5% 유의수준에서 0.999로 나타나 FMD 관련 보도가 1건 증가할 때마다 첫 구매율은 0.999배 감소한 것으로 분석되었다. 반면 2015년 돼지고기의 Haz. Ratio는 2010년과는 다르게 1.001로 나타나 오히려 첫 구매율이 증가한 것으로 분석되었다. 소고기의 경우에도 돼지고기와 비슷한 결과가 나타났다. 본 연구는 소비자의 육류소비에 질병 관련 보도가 첫 구매에 어떠한 영향을 미치는지에 대해 원산지별, 육류별로 추정하여 보다 합리적인 생산 및 소비 활동에 필요한 정보를 제공하는 데 의의를 가지고 있다. 또한, 축산 질병 관련 보도가 지속되는 시간을 추정하여 제시함으로써 소비 판촉 등의 홍보에도 반영할 수 있을 것으로 여겨진다.

• 한국건강관리협회지
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• 제2권1호
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• pp.93-104
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• 2004

Purpose : This study was conducted to understand the health status and health behaviors of preschoolers to provide baseline data for developing health promotion programs. Method 'Parents of children attending day-care centers and guardians of preschoolers were recruited for the study. Data were collected from June to August2002 using a structured questionnaire. Result . Among 754 preschoolers, 17,3% were overweight, while 18.2% were underweight, while 15.9% and 23.2% were overweight and underweight respectively, among 85 children living with guardians. The most frequent infectious diseases that children have had previously were hand-foot-mouth disease, chicken pox, and measles. Current health conditions that children have frequently are respiratory disease and atopic dermatitis for bother groups. Only 54%-61% brush their teeth everyday at bedtime, 49%-54% wash their hands every time after returning home, 4.5%-8.8%wear bicycle helmets, 9,3%-11.1% use a child car seat, and 3.5%-8.1% eat fruits and vegetables five times a day, Children living with guardians were less likely toper form positive health behaviors compared with children in day-care centers. Conclusion 'Based on the study results, health professionals could plan and develop health promotion programs to change unhealthy behaviors of preschoolers targeting high-risk groups.