• Journal of Microbiology and Biotechnology
• /
• 제26권8호
• /
• pp.1375-1382
• /
• 2016

The extremely halophilic archaeon Halobacterium noricense is a member of the genus Halobacterium. Strain CBA1132 (= KCCM 43183, JCM 31150) was isolated from solar salt. The genome of strain CBA1132 assembled with 4 contigs, including three rRNA genes, 44 tRNA genes, and 3,208 open reading frames. Strain CBA1132 had nine putative CRISPRs and the genome contained genes encoding metal resistance determinants: copper-translocating P-type ATPase (CtpA), arsenical pump-driving ATPase (ArsA), arsenate reductase (ArsC), and arsenical resistance operon repressor (ArsR). Strain CBA1132 was related to Halobacterium noricense, with 99.2% 16S rRNA gene sequence similarity. Based on the comparative genomic analysis, strain CBA1132 has distinctly evolved; moreover, essential genes related to nitrogen metabolism were only detected in the genome of strain CBA1132 among the reported genomes in the genus Halobacterium. This genome sequence of Halobacterium noricense CBA1132 may be of use in future molecular biological studies.

• Applied Biological Chemistry
• /
• 제33권3호
• /
• pp.247-251
• /
• 1990

오징어 젓갈로 부터 분리한 protease를 생산하는 H5-2 균주는 Halobacterium 속 균으로 동정되었으며 NaCl 2.0M 이하의 배지에서는 생육하지 못하는 고도 호염성균이었고, 이 균의 protease 생산은 Nan 4.5M, yeast extract 0.4%, gelatin 1.5%를 첨가한 pH7.0의 Norberg & Hofsten 배지에서 $38^{\circ}C$로 $4{\sim}5$일간 배양했을 때 가장 양호하였다.

• 한국미생물·생명공학회지
• /
• 제19권2호
• /
• pp.153-157
• /
• 1991

Intracellular enzymes of an extreme halophilic bacterium, Halobacterium sp. HE10, isolated from a saltern in Korea was investigated. The membrane-bound enzyme, NADH dehydrogenase, involved in electron transport system was stimulated by the addition of 2.0 M NaCl. The respiratory enzyme activities such as NADH oxidase and NADH dehydrogenase was decreased on removal of $Na^+$ ion and restored when replaced with cations like $K^+$, $Li^+$and $NH_{4}^{+}$ ions. Furthermore, their activities were affected by the anions such like carbonate, acetate, sulfate, chloride and nitrate at the presence of $Na^+$ion. Lactate dehydrogenase activity was highest at the asturated solution of NaCl and isocitrate dehydrogenase activity was a maximum level at 1.0 M NaCl. These results suggested that the enzyme activites of the respiratory chain in Halobacterium sp. EH10 was stimulated by the presence of $Na^+$ ion.

• KSBB Journal
• /
• 제13권3호
• /
• pp.268-271
• /
• 1998

Bacteriorhodopsin in the purple membrane (PM) of halobacteria has recently been attracting much attention to be used as a component of molecular electron device and optical computers. In order to increase the productivity of bacteriorhodopsin in high cell density cultures of Halobacterium halobium R1, an internal membrane cell-retention bioreactor system was employed. As a result, the production of cell mass at OD660 of 12 and of bacteriorhodopsin at 125-130 mg/L were obtained using the internal membrane bioreactor system at a dilution rate of 0.066 hr-1. The productivity achieved by the internal membrane system (0.7 mg/L$.$hr) was 3.5-fold higher than that obtained by the corresponding batch cultivations (0.2 mg/L$.$hr).

• 한국식품영양과학회지
• /
• 제23권5호
• /
• pp.856-862
• /
• 1994

In salt-preserved foods of every kinds, it was examined the growth condition of halophilic bacteria that induced a change of colour, taste, nutritive substance, a production condition of enzyme and a character of crude enzyme. Used bacteria is H. halobium a kind of extremely halophilic bacteria, and the required of optimum culture needed a quite long time of crude enzyme production is 168 hours. Optimum pH is about 7-7.5, so the traditional food of such neutrality pH as soybean paste and soy sauce particularly come into trouble because the growth can flourish in neutrality or alkaliescence, and the crude enzyme also appeared that best activation between pH 6 and pH 8. The optimum temperature is about 37$^{\circ}C$, the optimum temperature of enzyme is about 40 $^{\circ}C$ and the temperature stability is settled for 15 minutes and it is completely inactivated at 10 minutes. In the influence of each metal ion, Fe++ and Mn++ a stimulated the growth of H.halobium and the activation of enzyme, Cu++ and Zn++ were identified that made the growth and the activation of enzyme inhibit.

• 미생물학회지
• /
• 제29권1호
• /
• pp.56-62
• /
• 1991

Extremely halophilic bacteria isolated from salterns at Mado, Kyunggido, Korea, were identified and investigated on their salt requirements. The results have shown that six strains were identified to be belonged to the genus Halobacterium and three strains identified as the fenus Halococcus. Among them, the optimal NaCl concentration for growth of Halobacterium sp. EH10 was at 4.2M and no growth occurs below 2.0M NaCl. The strain, EH10, is nonmotile and showed acid production from glucose, fructose and maltose while H. salinarum is motile and does not produce acid from any carbohydrates. On the other hand, the strain EH10 does not utilize readily glucose while a number of sugars are readily utilized for growth with acid production by H. saccharovorum. Thus, the isolate, EH10, was classified into the genus Halobacterium and could be a novel species of the genus by its main morphological and physiological features including G+C content. The optimal temperature for growth of the isolate, EH10, was 50.deg.C. But this strain did not grow when NaCl was replaced with KCl.

• Applied Biological Chemistry
• /
• 제33권4호
• /
• pp.337-342
• /
• 1990

Halobacterium sp.가 생산하는 protease를 ethanol 침전, S데hadexG-100 및 G-75로 겔 여과하여 비활성이 364units/mg protein 수율이 14%로 정제할 수 있었다. 정제호소의 casein에 대한 Km값은 $4.2{\times}10^{-4}M$이었다. 이 효소의 반응 최적온도 및 pH는 $35^{\circ}C$와 pH 8.0이었고, 온도 및 pH에 대한 안정성은 $50^{\circ}C$까지와 pH $5.0{\sim}11.0$범위이었다. $Ca^{2+}$와 $Mg^{2+}$ 이온은 효소의 활성을 증가시켰고 $Fe^{3+},\;Zn^{2+},\;Cu^{2+},\;Hg^{2+},\;Cd^{2+}$ 이온은 저해하였다. 정제호소는 기질용액중에 NaCl농도가 증가함에 따라 효소활성이 감소하고 2M NaCl 농도 이하에서 투석하면 활성이 저하되었으며 증류수로 2시간 투석으로 완전히 실활되었다. 그러나 0.1M $CaCl_2$용액으로는 6시간 투석하여도 활성은 그대로 유지되었다.

• 한국응용과학기술학회지
• /
• 제13권3호
• /
• pp.119-125
• /
• 1996

The excess volumes of mixing of benzyl alcohol, ethyl alcohol, halothane, and procaine in vesicle and suspensions of several lipids have been determined at $25^{\circ}C$ using a excess volume dilatometer. The potency of general anesthetics has long been known to be correlated with lipid solubility. Denaturations of the bacteriorhodopsin, which is a sole membrane protein in the purple membrane of Halobacteriun Halobium, were studied by UV/Vis absorption changes. The excess volumes of mixing of benzyl alcohol and procaine in egg lecithin were all found to be negative and this result was confirmed as Miller's supposition.

• 한국응용과학기술학회지
• /
• 제35권1호
• /
• pp.157-162
• /
• 2018

Halobacterium Halobium에서 항우울제의 부분 몰 부피와 몇 가지 지질의 부유물은 과량의 부피 팽창 계를 사용하여 $25^{\circ}C$에서 측정되었다. 일반적인 항우울제 인 Fluoxetine의 효능은 오랫동안 지질 용해도와 관련이 있는 것으로 알려져 왔다. Halobacterium Halobium의 보라색 막에서 유일한 막 단백질 인 소포의 변성은 280nm에서 흡수 변화에 의해 연구되었고 과도한 부피 팽창 계로 330nm에서 형광 변화를 보였다. 확산 교환에 의한 점성 중합체 용액의 1H NMR 분석은 측정을 위한 중요한 단계로, Halobacteriun Halobium에서 Fluoxetine의 부분 몰 부피와 입자 크기는 양성으로 측정되었다. 항우울제는 낮은 사기와 우울증에 근거하여 다양한 인지 및 정신 증상을 유발하는 질병을 예방하여 일일 수행 능력을 저하시키는 걸로 나타났다.

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2005년도 생물공학의 동향(XVII)
• /
• pp.621-621
• /
• 2005