• Journal of Periodontal and Implant Science
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• v.37 no.1
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• pp.1-10
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• 2007

연구 목적 : 구취의 주된 화학적 요소는 휘발성 황 화합물 (VSCs)들이다. 이들은 치태, 설태, 타액과 혈액, 상피세포의 혐기성 그람 음성 세균에 의한 단백분해 변성으로 만들어 진다. 또한 구취는 치주질환과 타액내의 세균의 증가를 야기하는 불량한 구강 위생상태에서 기인한다고 보고되고 있다. 이 연구의 목적은 VSCs를 측정함으로 구취에 대한 비외과적 치주 치료의 영향을 평가하고, 타액내의 세균 수를 측정함으로써 VSCs와의 상관관계를 살펴보고자 하는 것이다. 연구 대상 및 방법 : 중등도의 만성치주염을 가지는 23명의 참가자들이 대조군과 실험군으로 분류되었다. 대조군은 구강위생교육 (치간청결기구와 혀세정 포함)만을 시행하였고 실험군은 구강위생교육과 함께 24시간 내에 전악 치석제거술과 치근활택술을 시행하였다. 치료 전과 치료 4주 후에 VSCs 농도를 $Halimeter^{(R)}$(Interscan Corp., CA, USA)를 사용하여 측정하였고 타액 시료는 혐기성과 호기성 조건에서 배양되었다. 결과 : 4주 후에 두 군 모두에서 휘발성 황화합물의 농도가 유의하게 감소하였고, 실험군에서의 감소량이 통계적으로 유의하게 더 크게 나타났다. 타액 내 세균의 배양에서 총세균수에 대한 혐기성 세균의 비율이 실험군에서만 통계적으로 유의하게 감소하였다. 그러나 세균의 비율과 휘발성 황화합물의 농도와의 상관관계는 낮게 나타났다. 즉 구취의 감소는 타액 요인 보다는 치주치료에 의한 다른 복합적인 인자의 작용에 의한 것으로 추론할 수 있었다. 결론 : 본 연구는 중등도의 치주염환자에서 구강위생교육만으로도 구취의 주요 화학적 요인인 휘발성 황화합물의 농도를 감소시킬 수 있으나 비외과적 치주치료를 동반하였을 때 더욱 큰 효과를 얻을 수 있는 것으로 해석된다. 그러나 실험 참가자의 수가 적었고 장기간의 분석이 이루어지지 않았으므로 앞으로 더 많은 연구가 구취에 대한 치주치료의 영향을 이해하기 위해서 필요할 것으로 사료된다.

• The Journal of Internal Korean Medicine
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• v.25 no.4
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• pp.252-259
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• 2004

Objectives : Halitosis is a common human condition, exact pathophysiological mechanisms of which are unclear. This study, which investigates halitosis patients' characteristics and their related factors, was done with intent to establish a foundation for the Oriental Medical treatment of halitosis. Methods : 329 patients were surveyed by reviewing medical charts and questionnaires from the East-West Halitosis Clinic of Kyunghee Medical Center from May 1, 2001 to December 31, 2002. Sex, age, illness duration, coating on the tongue, postnasal drip, globus pharyngeus, indigestion, condition of feces, halimeter measurements and results of the salivary scans were analyzed. Results : The thick and yellowish coating on the tongue was not a major cause of halitosis in the patients studied. Among the halitosis patients, 21.6% complained of postnasal drip, 15.6% of globus pharyngeus, 37.4% of indigestion, and 23.8% of diarrhea or constipation, suggesting the possibility of extra-oral origin in oral malodor. Conclusions : This study suggests that, when treating halitosis patients, intra-oral causes of halitosis aside, Oriental Medical treatments such as solving qi-stasis(氣鬱), augmenting deficiency of the spleen qi(碑氣虛) and treating gastric fever(胃熱) constitute effective management for oral malodor patients.

• The Journal of Internal Korean Medicine
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• v.28 no.2
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• pp.354-362
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• 2007

Objective : The aim of this study was to investigate the ability of Cheonggugamrosu. a gargling agent made from herb extracts, to decrease oral malodor in vitro and in clinical research. Methods : For in vitro experiment. samples were Garglin (over-the-counter mouthwash), CheonggugamrosuA and Cheonggugamrosu B. Deodorizing activity of each sample against methyl mercaptan were analyzed by gas chromatography (GC). In clinical examination. subjects were 73 students from the oriental medicine school of Kyunghee University. The concentration of VSC in oral cavity of each subject was measured by Halimeter and subjects were divided into 3 groups that would gargle for 30 seconds with normal saline. Garglin and CheonggugamrosuA. Subsequently, concentration of VSC was measured in same way immediately, 2 hours and 4 hours after gargling. Results : During in vitro experiment by GC, deodorizing activities were 46.77% with Garglin, 80.64% with Cheonggugamrosu A and 82.28% with Cheonggugamrosu B. In clinical examination, at 2 hours after gargling, the concentration of VSC in the Cheonggugamrosu A group was significantly lower than in the normal saline group, but not in the Garglin group. 4 hours after gargling, the concentration of VSC in both CheonggugamrosuA and Garglin wasn't significantly different from the one in the normal saline group. Conclusions : Cheonggugamrosuhad better and longer deodorizing effect than Garglin in this study. Because of the safety of herbal medicine and its efficaciousness, we can use Cheonggugamrosu as a clinical prescription.

• Journal of Periodontal and Implant Science
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• v.30 no.1
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• pp.203-212
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• 2000

Putrefactive activity within the oral cavity is the principal cause of halitosis. The most common intraoral sites of oral malodor production are tongue, interdental and subgingival areas. The other foci may include faulty restorations, sites of food impaction and abscesses. Periodontal disease frequently involves pathological oral malodor, which is caused mainly by volatile sulfur compounds(VSC), such as hydrogen sulfide, methyl mercaptan, and dimethyl sulfide. The purpose of this study is to evaluate the association between oral malodor and periodontal status. Volatile sulfur compounds in mouth air were estimated by portable sulfide monitor($Halimeter^{TM}$). The results were as follows : 1. The levels of volatile sulfur compounds were significantly greater in a periodontitis group than in a control group(P<0.01). The amounts of VSC in mouth air from patients with periodontal involvement were four times greater than those of the control group. 2. The significant positive correlation was found between VSC concentrations and the number of pocket depth above 4mm(P<0.01), but correlation between VSC concentrations and plaque score was not statistically significant(P>0.05). 3. In the periodontitis group, VSC concentrations of pre-treatment significantly decreased after scaling and root planing(P<0.01). 4. No statistically significant correlation was found between VSC concentrations and sex / age in the periodontitis group. The above results indicate that periodontal disease may play a role as an important factor of oral malodor and deep periodontal pockets are a source of volatile sulfur compounds.

• Journal of Microbiology and Biotechnology
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• v.29 no.2
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• pp.311-320
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• 2019

Fusobacterium nucleatum is a morbific agent in periodontitis and halitosis. Egg yolk antibody (IgY) was obtained from egg yolks from chickens stimulated with F. nucleatum. This study was to assess the effectiveness of IgY on periodontitis and halitosis caused by F. nucleatum in vitro and in vivo. The growth of F. nucleatum was inhibited (p <0.05) by different concentrations of IgY in vitro and the results of a Halimeter show volatile sulfur compounds (VSCs) were reduced to $904{\pm}57ppb$ at a concentration 40 mg/ml of IgY. The changes of fatty acids of F. nucleatum were determined using GC-MS. The scores for odor index of rat saliva were decreased. The major constituent of volatile organic compounds (VOCs) including short-chain acids decreased 46.2% in 10 mg/ml IgY, ammonia decreased 70% in 40 mg/ml IgY, while aldehydes and olefine ketones were almost unchanged. The ELISA assay revealed that IL-6 and TNF-${\alpha}$ were decreased after 4 weeks' IgY treatment. Morphometric (X-ray) and histological analyses (HE) showed that IgY reduced alveolar bone loss and collagen fibers became orderly in rat models. As a result, IgY may have the potential to treat periodontitis and halitosis.

• The Journal of Korean Medicine
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• v.31 no.4
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• pp.151-163
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• 2010

Objective: We investigated the oral malodor inhibitory effect of Scutellariae Radix (SR), Phellodendri Cortex (PC), Moutan Cortex (MTC) and Magnoliae Cortex (MGC) for the development of a gargle solution. Methods: 1. Against P. gingivalis and Pr. intermedia, the minimal bactericidal concentration (MBC) and the change of viable cells that were exposed to 1% each herbal extract were observed. 2. Deodorizing activity of 2% herbal extract and Garglin $Mint^{(R)}$ against methyl mercaptan were evaluated by gas chromatography (GC). 3. We used the salivary sediment system (SSS) as the malodor model. 4. The clinical examination was repeated 3 times by 2 subjects by $Halimeter^{(R)}$. Baseline VSC of each subject was measured. Then, the control subject gargled with cysteine for 30 sec. After 4 min, subjects would gargle for 30 seconds with herbal extracts (2%) and Garglin $Mint^{(R)}$. Subsequently, the concentration of VSC was measured at 0, 4, 8, 12, 16, 20, 40 and 60 minutes. Results: 1. Against P. gingivalis, MBC of SR, PC and MTC was 0.1%, and MBC of MGC was 1%. Removal time of P. gingivalis was as follows; 5 hr in MGC, 24 hr in SR and PC, and 48 hr in MTC. Against Pr. intermedia, MBC of SR and PC was 0.5%, and MBC of MTC, MGC was 1%. Removal time of Pr. intermedia was as follows; 5 hr in MTC and 24 hr in SR, PC and MGC. 2. Deodorizing effect of herbal extracts against methyl mercaptan was as follows; MGC and MTC had 100%, SR had 82.22%, PC had 66.60%, Garglin $Mint^{(R)}$ had 40.54%. 3. In the experiment using SSS, PC and MTC had statistically significant malodor-inhibitory effects (p<.05). 4. In the clinical examination, PC and MGC had statistically significant inhibitory effects at every elapsed time compared to the control subject. MTC had that until 40 min. SR had that at 0, 4, 8, 20, and 60 min. Conclusions: SR, PC, MTC and MGC have an antibacterial effect and the chemical removable activity of the oral malodor caused by VSC. These four herbs could have potential as effective anti-malodor agents.

• Journal of Oral Medicine and Pain
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• v.36 no.4
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• pp.245-252
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• 2011

The aim of this study is to clinically evaluate the effect of a mouthwash containing cetylpyridinium and zinc chloride on reduction of morning oral malodor in healthy subjects measured by organoleptic measurement and a portable sulfide monitor. A total of 8 healthy non-smoking male volunteers were enrolled in this study of crossover design consisting of two experimental phases. The subjects were instructed to rinse the mouth with 10 mL of the experimental mouthwash containing cetylpyridinium and zinc chloride for 30 seconds at the first phase. At the second phase after a one-week washout period, each subject rinsed with distilled water as a control. All experiments were conducted at around 8:30 a.m. and oral malodor was measured using organoleptic measurement and a portable sulfide monitor just before rinsing with the experimental mouthwash or control (baseline), 1 hr, 2 hrs, and 3 hrs after rinsing. The mouthwash containing cetylpyridinium and zinc chloride reduced morning oral malodor up to 3 hrs after rinsing. Organoleptic score and concentrations of volatile sulfur compounds after use of the experimental mouthwash significantly decreased with time, and the decreases were significantly different between the mouthwash and control. In conclusion, the mouthwash containing cetylpyridinium and zinc chloride is significantly effective on reduction of morning oral malodor in healthy subjects by 3 hrs.