• Molecules and Cells
• /
• 제38권6호
• /
• pp.528-534
• /
• 2015

Hypoxia-inducible factor (HIF) is a key regulator of tumor growth and angiogenesis. Recent studies have shown that, BIX01294, a G9a histone methyltransferase (HMT)-specific inhibitor, induces apoptosis and inhibits the proliferation, migration, and invasion of cancer cells. However, not many studies have investigated whether inhibition of G9a HMT can modulate HIF-$1{\alpha}$ stability and angiogenesis. Here, we show that BIX01294 dose-dependently decreases levels of HIF-$1{\alpha}$ in HepG2 human hepatocellular carcinoma cells. The half-life of HIF-$1{\alpha}$, expression of proline hydroxylase 2 (PHD2), hydroxylated HIF-$1{\alpha}$ and von Hippel-Lindau protein (pVHL) under hypoxic conditions were decreased by BIX01294. The mRNA expression and secretion of vascular endothelial growth factor (VEGF) were also significantly reduced by BIX01294 under hypoxic conditions in HepG2 cells. BIX01294 remarkably decreased angiogenic activity induced by VEGF in vitro, ex vivo, and in vivo, as demonstrated by assays using human umbilical vein endothelial cells (HUVECs), mouse aortic rings, and chick chorioallantoic membranes (CAMs), respectively. Furthermore, BIX01294 suppressed VEGF-induced matrix metalloproteinase 2 (MMP2) activity and inhibited VEGF-induced phosphorylation of VEGF receptor 2 (VEGFR-2), focal adhesion kinase (FAK), and paxillin in HUVECs. In addition, BIX01294 inhibited VEGF-induced formation of actin cytoskeletal stress fibers. In conclusion, we demonstrated that BIX01294 inhibits HIF-$1{\alpha}$ stability and VEGF-induced angiogenesis through the VEGFR-2 signaling pathway and actin cytoskeletal remodeling, indicating a promising approach for developing novel therapeutics to stop tumor progression.

• 복식
• /
• 제66권6호
• /
• pp.95-109
• /
• 2016

The process of making fashion accessories brings many benefits to visually impaired people. It helps the development of their tactile sensation, improves their aesthetic attitude, creativity, and imagination, and brings energy to their life. The current accessory making education in Korea is targeted to people with normal vision, and does not reflect the specific needs of the visually impaired. This causes a lot of trouble in educating the visually impaired. Therefore, this study aims to increase the efficiency of accessory making education by selecting curriculum and material that are appropriate and systematic for the visually impaired. In order to understand visual impairment, literature review was conducted to examine the definition and classifications of the impairment. Research was also done regarding material types and production method of fashion accessory. The study also designed an education program, including teaching guidelines, by referring to literature related to fashion accessory design, teaching guideline. The study conducted 12 experiments based on the results from April to June 2014. After revising and supplementing the teaching method, 2nd set of experiments were conducted from July to September 2014. Each class lasted for one and a half hour every week, and the class was composed of 6 students, 1 instructor and 1 volunteer. After each class, interviews were done to collect student opinions about the curriculum and material. Total experiment results and interview were reflected in constructing the final education program. The result of the experiments and interviews showed that selecting the accessory materials for class must be selected carefully. The visually-impaired students were very sensitive to the size, texture, shape, hole size of beads and length of material. After the experiments, all the students were able to distinguish the size and shape of the beads well, and finished the accessories using the given materials. The study verified that there is a need for another approach when educating the visually impaired about fashion accessory design. Many factors need to be considered from selecting materials to class contents. This study will contribute as a reference to fashion accessory making education as well as other design education for the visually impaired.

• 인삼문화
• /
• 제2권
• /
• pp.17-26
• /
• 2020

인삼은 예로부터 연명(延命)의 약물로 분류된 상약(上藥)의 하나로 자리를 잡고 지닌 약성에 따라 만병통치약으로 사용되었으며, 특수계급만을 대상으로 하였던 인삼 수요가 다른 계층으로 확대되면서 그 수요가 급증하게 되었다. 이에 따라 무분별한 채취로 멸절(滅絶)되어 채취하기가 어렵게 되어 자연산 인삼으로는 공급의 한계에 이르게 되자, 인삼 공급 방법은 힘든 자연산 인삼의 채취보다는 가성비 높은 가삼 재배 쪽으로 이동하게 되었다. 인삼종주국인 우리나라의 가삼 재배 시기에 관해서는 연구가 이루어지고 있으나, 첫 재배지에 관한 연구는 아직 보고되지 않고 있다. 본 연구에서는 가삼 재배 관련 자료의 정리를 통하여 우리나라 최초의 가삼 재배지에 관한 정리와 고찰을 하였으며, 여기에 인용된 사료는 조선왕조실록, 임원십육지, 오주연문장전산고, 중경지, 증보문헌비고, 인삼사 등이다. 정리와 고찰의 결과, 조선 시대 최초의 가삼 재배지는 영남지역으로 특히, 풍기면(현재의 경북 영주시 풍기읍)으로 추측된다.

• 한국미생물·생명공학회지
• /
• 제36권3호
• /
• pp.182-188
• /
• 2008

내열성 Trehalose synthase를 생산하는 초고온성 균주 HJ6은 일본 Arima 온천수에서 분리하였다. 세포의 길이는 $2{\sim}4\;um$, 직경 0.4 um의 간균으로 생육최적 pH와 온도는 각각 6.5와 $80^{\circ}C$이였다. 분리된 균주의 16s rRNA 염기서열을 분석하고 계통학적으로 분류한 결과, HJ6 균주는 Thermus thermophilus에 속하는 것으로 동정되었다. PCR법을 이용하여 trehalose synthase(TS) 유전자를 클로닝하고 염기서열을 분석한 결과, ORF는 2,898개의 뉴클레오타이드로 구성되고 915개의 아미노산을 암호화하였다. 아마노산 서열을 바탕으로 상동성을 분석한 결과, Thermus caldophilus GK24 유래 TS와 99%, Meiothermus ruber 유래 TS와 83%의 identity를 나타내었다. 이 유전자를 온도감수성 프로모터를 포함하는 pJLA503 벡터를 이용하며 대장군에서 발현하고 정제하여 약 110 kDa 단백질을 얻을 수 있었다. 정제된 효소는 트레할로스 전환활성에 대한 최적 pH가 7.5이고, 최적온도는 $80^{\circ}C$이며, 활성의 반감기는 $90^{\circ}C$에서 40분으로 확인되어 높은 내열성을 가지는 것으로 확인되었다. 본 효소의 트레할로스 최대 전환율은 기질농도 500mM에서 55.7%를 나타내었고, 기질 농도가 증가함에 따라 더불어 증가하였기 때문에 본 효소의 트레할로스 전환율을 기질농도에 의존적인 것으로 생각되었다.

• 대한약리학회지
• /
• 제25권1호
• /
• pp.101-107
• /
• 1989

Procainamide를 투여후 이 약물 및 활성형 대사산물인 N-acetylprocainamide (NAPA)의 약동학적 성상을 알아보기 위해 숫컷 성견 5마리에 procainamide 및 NAPA를 교차 투여하여 얻은 혈장농도 data를 2-compartmental model에 의해 약동학적 분석을 시행하여 다음과 같은 결과를 얻었다. 1. 10mg/kg의 procainamide를 1회 15분간 정주후 혈장 procainamide 농도변화는 명백한 분포기와 소실기를 보였으며 생성된 NAPA의 혈장농도는 시간경과에 따라 최고혈장농도는 $0.124{\mu}g/ml$ 이하이었으며 정주 직후 조직분포에 따른 혈장농도의 일시적으로 감소 후 증가하는 초기 dip 현상을 보였다. 2. Procainamide의 steady-state 분포용적(Vss) 및 central compartment volume (Vc)은 각각 $1.20{\pm}0.27\;L/kg$ 및 $0.36{\pm}0.08\;L/kg$ 이였으며 NAPA의 Vss및 Vd는 $1.21{\pm}0.21\;L/kg$ 및 $0.26{\pm}0.07\;L/kg$이었다. 3. Procainamide 및 NAPA의 청소율(Cl)은 각 $0.47{\pm}0.08\;L/kg/hr$와 $0.35{\pm}0.08\;L/kg/hr$ 이었으며 혈장 반감기$(t_{1/2{\beta}})$는 각각 2.85 및 2.77 시간이었다. 4. N-acetylation에 의한 Procainamide의 대사청소율은 $18.24{\pm}6.22\;ml/kg/hr$로 이는 전체 procainamide 청소율의 3.9%를 차지하였다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
• /
• 제35권6호
• /
• pp.397-402
• /
• 2009

The purpose of this study is to investigate the effects of alendronate and pamidronate on proliferation and the alkaline phosphatase activity of human bone marrow derived mesenchymal stem cells and to relate the results with bisphosphonate related osteonecrosis of the jaw(BRONJ). With the consent of patients with no systemic disease and undergoing iliac bone graft, cancellous bone was collected to obtain human bone marrow derived mesenchymal stem cells through cell culture. 96 well plate were prepared with a concentration of $10^4$cell/ well. Alendronate and pamidronate were added to each well with the concentration of $10^{-6}M$, $10^{-8}M$ and $10^{-10}M$, respectively. Then proliferation capacity of each well was evaluated with the cell counting kit. 24 well plates were prepared with a concentration of $10^5$cell/ml/well and with the bone supplement, alendronate and pamidronate were added with the concentration of $10^{-6}M$, $10^{-8}M$ and $10^{-10}M$, respectively on each plate. The plates were cultured for either 24 or 72 hours. Then the cells were sonicated to measure the alkaline phosphatase activity and protein assay was done to standardize the data for analysis. As the concentration of alendronate or pamidronate added to the culture increased, the proliferation capacity of the cells decreased. However, no statistical significance was found between the group with $10^{-10}M$ of bisphophonate and the control group. Pamidronate was not capable of increasing the alkaline phosphatase activity in all trials. However, alkaline phosphatase activity increased with 24 hours of $10^{-8}M$ of alendronate treatment and with 48 hours of $10^{-10}M$ of alendronate treatment. Cell toxicity increased as the bisphosphonate concentration increased. This seems to be associated with the long half life of bisphosphonate, resulting in high concentration of bisphosphonate in the jaw and thus displaying delayed healing after surgical procedures. Alendronate has shown to increase the alkaline phophatase activity of human bone marrow derived mesenchymal stem cells. However, this data is insufficient to conclude that alendronate facilitates the differentiation of human bone marrow derived mesenchymal stem cells. Further studies on DNA level and animal studies are required to support these results.

• Applied Biological Chemistry
• /
• 제40권1호
• /
• pp.76-79
• /
• 1997

국내에서 최초로 합성, 개발된 유기인계 살충제인 flupyrazofos(KH-502)의 물리화학적 특성으로 수용성, 가수분해, 증기압, 분배계수를 EPA와 OECD 방법에 준하여 측정하였다. 수용성은 $25^{\circ}C$에서 0.80 ppm으로 낮았고 가수분해 반감기는 $25^{\circ}C$에서 266.5시간(pH 4.0), 180.0시간(pH 7.0), 120.9시간(pH 9.0)으로 알칼리 조건에서 불안정하였으며 $40^{\circ}C$에서는 $25^{\circ}C$보다 $2{\sim}4$배 정도 가수분해가 빠르게 진행되었다. 상은($25^{\circ}C$)에서의 증기압은 $2.81{\times}10^{-5}$ torr로 측정되었고, 다른 두 온도(35, $45^{\circ}C$)에서 증기압을 측정하여 log P=0.673-(1565.4/T)라는 관계식을 구하였다. Flupyrazofos의 상온($25^{\circ}C$)에서의 증기압은 diazinon과 유사한 수준이었고, DDVP보다는 약 1,000배 정도 낮은 수치로 휘발에 의하여 환경에 영향을 미칠 가능성은 낮을 것으로 판단되었다. Flupyrazofos의 옥탄올/물 분배계수치를 보면(log Kow=5.24) 먹이연쇄를 통한 생물농축이 예상되나 비교적 가수분해가 빠르기 때문에 비록 수계에 노출된다고 하여도 그 가능성은 매우 낮을 것으로 사료된다.

• 한국보건간호학회지
• /
• 제15권2호
• /
• pp.225-238
• /
• 2001

The purpose of this study is to examine public health nurses for their morale, test the effect of each factor on the morale, determine the variables affecting the morale, and thereby, provide for some basic data useful to the development of personnel affair administration for nurses. The survey on the nurses for public health centers was conducted for 10 days from April 23 to May 2, 2001; 96 nurses from Pusan, 101 from Kyonggi-do and 108 from Kangwon-do. The results of the study are as follows; 1. For the demographic variables, those aged between 31 and 39 accounted for the majority $(50.2\%)$ of the sample. $90.8\%$ was married, and $67.9\%$ of the sample graduated from junior colleges. $29.6\%$ of them had worked for 10-14 years and $62.5\%$ was of the seventh rank. $62.3\%$ of the nurses was recruited, and $59.4\%$ had chosen the public job for its guaranteed status. Half the nurses were working for the county population of which was more than 260 thousands, and $53\%$ of the nurses was serving the public health centers with 11-15 members. $47.3\%$ of them perceived that their living standard is middle or lower. 2. The degree of the moral was found as mean score. 2.65, which is interpreted as low. It has 11 factors. The rank of mean score of the factors is job satisfaction (3.36), sense of belonging (3.36), sense of recognition (3.30), stress due to supervision (3.13), degree of participation (3.05) and self-achievement (3.04), while those scoring lower points were assurance of status (2.58), pay level (2.53), working conditions (2.35) and promotion opportunity (2.31). 3. The degree of moral depending on the demographic variables was significantly affected by such variables as age. academic background, career, rank, employment method, motive for the job, size of the population served and living standards. 4. The result of the Stepwise multiple regression analysis for the morale factors, it was found that self-achievement accounted for $61\%$ of the total variance of the degree of morale, followed by stable status $(18\%)$, working conditions $(8\%)$, job requirements $(4\%)$ and promotion opportunities $(2\%)$; these five factors explained about $94.6\%$ of total variance of the degree of nurses' morale. On the other hand. age $(8\%)$ and academic background $(2\%)$ accounted for $10\%$ of the total variance of the degree of morale. 5. The factors affecting nurses' job life was economic need $(26.7\%)$, personnel management $(17.5\%)$, sense of recognition $(13.2\%)$, human relationship $(11.0\%)$, stable status and personal development in rank. On the other hand, the factors influencing on their possible resignation were children's bringing-up $(27.8\%)$, poor personnel management and promotion $(13.3\%)$, workload $(11.1\%)$ and little personal development $(8.9\%)$, Since such morale factors as promotion opportunity, working condition. pay level and stable status scored lower, it is required to reconsider those factors to improve nurses' morale.

• 보건교육건강증진학회지
• /
• 제12권2호
• /
• pp.73-89
• /
• 1995

This study was planned to investigate indirectly the effects of study environments of high school students such as family characteristics, availability of sex education, and possibility of exposure to unhealthy sexual environments. For this purpose, the level of knowledge and attitude towards sex of general and industrial high school students were compared because these two groups of students were believed to be different in those study variables. For this study, 600 students (300 male and 300 female students) from a general high school students and 400 (200 male and 200 female) from an industrial high school in one industrial city in Korea were surveyed with a structured questionnaire. The study results were as follows: 1. There were significant differences in selected family condition variables between general and industrial high school students. Proportions of students with single parents, those who live in houses other than their own, and those father's occupations were farming and manufacturing were higher in industrial high school students than in general high school students. 2. Female students wanted to have more knowledge on sex whereas male students were interested in things related to sexual acts in both general and industrial high school students. The largest proportion of students wanted to discuss their sex problems with their friends not with their parents. 3. More students in general high school thought that the sexual intercourse is natural and pretty than those in industrial high school, whereas more industrial high school students insisted on the sexual purity before marriage than general high school students. 4. About 65% of students surveyed agreed to making friends with opposite sex and, in fact, more than half either had experiences or were having friends of opposite sex. More students in industrial high school wanted to choose their marital partners based on advices of their parents than students in general high school. 5. More female students than male and more industrial high school students than general high school students were embarrassed with first wet dream or menstruation and felt guilty about the masturbation. 6. Level of knowledge on reproductive physiology, sex transmitted disease and contraception was higher in general high school students than in industrial high school students. These study results suggest that simple knowledge on sex of the general high school students does not seem to affect positively the students attitude towards sex and the unfavorable living conditions of industrial high school students seem to influence the students to become more sincere and healthy in their attitude towards sex and life. Therefore, more thoughtful consideration in contents and methods should be given when the sex education is provided to the high school students.

• 가정과삶의질연구
• /
• 제19권4호
• /
• pp.49-68
• /
• 2001

The purpose of this study was 1) to review communal housing in the UK, 2) to consider the policy implications for elderly communal housing in Korea. The research methods used were 1) literature review about communal housing and related policy in the UK 2) field survey in the UK 3) interpretative suggestion for the proper policy implication to develope communal housing for the elderly in Korea. Sheltered housing in the UK had been developed as communal housing for the elderly with special needs since the 1970s. The type of sheltered housing were category 1 and category 2. Very sheltered housing with more facilities and meal services was added in 1980s. Sheltered housing was evaluated as the most humanistic solution for older people in the UK in 1980s. Because of the policy of moving institutional care to community care, sheltered housing became less in demand because of more options for older people including being able to stay in their own home. So new completion of sheltered housing by registered social landlords reduced saliently. Sheltered housing already totalled over half million units in which 5% of all elderly over 65 still lived and a small quantity of private sector for sale schemes emerged in the 1990s. The reason why the residents moved to sheltered housing was for sociable, secure, and manageable living arrangements. In general the residents were satisfied with these characteristics but dissatisfied with the service charge and quality of meals, especially in category 2.5 schemes. The degree of utilisation of communal spaces and facilities depended on the wardens ability and enthusiasm. Evaluation of sheltered housing indicated several problems such as wardens duty as a \"good neighbour\" ; difficult-to-let problems with poor location or individual units of bedsittiing type with shared bathroom ; and the under use of communal spaces and facilities. Some ideas to solve these problems were suggested by researchers through expanding wardens duty as a professional, opening the scheme to the public, improving interior standards, and accepting non-elderly applicants who need support. Some researchers insisted continuing development of sheltered housing, but higher standards must be considered for the minority who want to live in communal living arrangement. Recently, enhanced sheltered housing with greater involvement of relatives and with tied up policy in registration and funding suggested as an alternative for residential care. In conclusion, the rights of choice for older people should be policy support for special needs housing. Elderly communal housing, especially a model similar to sheltered housing category 2 with at least 1 meal a day might be recommended for a Korean Model. For special needs housing development either for rent or for sale, participation of the public sector and long term and low interest financial support for the private sector must be developed in Korea. Providing a system for scheme managers to train and retrain must be encouraged. The professional ability of the scheme manager to plan and to deliver services might be the most important factor for the success of elderly communal housing projects in Korea. In addition the expansion of a public health care service, the development of leisure programs in Senior Citizens Centre, home helper both for the elderly in communal housing and the elderly in mainstream housing of the community as well. Providing of elderly communal housing through the modified general Construction Act rather than the present Elderly Welfare Act might be more helpful to encourage the access of general people in Korea. in Korea.