The purpose of this study was to determine the effect of high voltage pulsed current (HVPC) stimulation on proliferative activities of basal keratinocytes by measured nucleolar organizer region (NOR) expression and thickness of spinous layer in aged rat skin. Fifty-one weeks old twelve male Sprague-Dawley rats (300∼350g) were divided into control and HVPC stimulation groups. Each animal's hair on the back were removed. The HVPC stimulation group received an negative monophasic twin peak pulsed current stimulation with 50 V, while the control group was given the same treatment without electricity. The rats were sacrificed after 3 weeks. The biopsy specimens were fixed in formalin, embedded in paraffin and stained with hematoxyline-eosin and silver nitrate. The thickness of basal to granular layer of the epidennis were measured using a light microscope and computerized image analysis system. The number of argyrophilic nucleolar organizer region (AgNOR) were counted using a light microscope and computerized image analysis system and calculated as the mean number of AgNOR per nucleus in the basal keratinocyte. By using a Student's t-test, an increase in the thickness of basal-spinous layer (P<0.001) of epidermis can be observed in HVPC stimulation rats as compared with the control rats, whereas the thickness of the granular layer is not affected. A Student's t-test showed a significantly higher mean NOR number per nucleus of the basal keratinocyte in the HVPC stimulation rats than control rats (P<0.001). There was significantly positive correlation between the NOR number and the thickness of basal-spinous layer (r=0.80, P<0.05). These results suggest that the HVPC stimulation may increase the thickness of spinous layer in the epidennis due to increased proliferative activities of basal keratinocytes in epidennis in aged rat skin.
This study attempts to analyze relational structures among service failure-related variables after the moderation of fairness in the beauty service industry with the following purposes: First, it aims to review and investigate service failure & service recovery strategies, non-switching intentions after recovery, revisit intention, the intent to provide word-of-mouth recommendations and previous studies on service failure and recovery in the beauty service industry. Second, it targets the analysis of the role of fairness as a variable that moderates relations between service recovery strategies and post-recovery satisfaction in the beauty service industry. For this, the following research method was used: This study has investigated the effect of service failure and its recovery strategies (behavioral recovery strategy, psychological recovery strategy, monetary recovery strategy) on customer satisfaction for beauty service users and used the Structural Equation Model (SEM) to further analyze and verify the effect of the satisfaction on post-satisfaction behavior (non-switching intention, repurchase intention and the intent to provide word-of-mouth recommendations). The SEM was divided into a measurement model and structural model to determine if the model is appropriate and estimate the parameters of the path coefficient. In addition, this study examined to see if fairness (procedural fairness, distributive fairness and interactive fairness) works as a moderating variable while the service recovery efforts affect customer satisfaction. Then, the role of service recovery strategies, targeted to satisfy the customers who were dissatisfied because of service failure, were investigated. In addition, its effect on post-satisfaction behavior was analyzed from the structural aspect, and the moderating role was examined as well. Then, the role of the service recovery strategy, which can be used to satisfy dissatisfied customers, was examined, and the effect of the satisfaction on customer behavior was analyzed from a structural perspective. In addition, the moderating role of fairness was tested. As a result, this study is significant in that it helps service providers formulate service recovery-related strategies.
Kim, Mi-Heui;Park, Duk-Byeong;Ahn, Yoon-Soo;Jun, Young-Mi
The Korean Journal of Community Living Science
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v.17
no.4
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pp.175-197
/
2006
This study aims to assess the traditional knowledge on seasonal customs and its characteristics for practical use. The Delphi method and correspondence analysis were hired to collect and analysis the data. Twenty six professionals for the Delphi participated in three-round process. The followings were concluded. The number of items valuing and resourcing for seasonal customs. were 118(40 for folks and ceremonies, 53 for foods. 25 for plays) on Delphi results. For example are Bokjori, Dano fm, Bokjumeoni, Chuseok, Tano festival, New Year Card, washing hair with an iris, Soup with rice cake, Boiled rice with five cereals, Rice and red-bean porridge, Kimchi-making for the winter, Sharing walnut with friends, Game of yut, Playing kite, Play with Hanga etc. Nowadays the ideas of modernized practical use on seasonal customs was to connect with modernized scientific technology and designate commemoration day. The items for connecting with modernized scientific technology were new year card, painting and recording sound with traditional seasonal customs, food areas, cake with seven kind of cereals, play areas, hand wrestling, cockfighting in folks and customs areas. Also the items for designating commemoration days were the Suelbeam and Suelbeam Socks present for the aged people, man cooking day, Korean traditional workers day in folk and ceremony areas.
Purpose: The Inaba's procedure, the treatment of osmidrosis axillae, has the advantages of low recurrent rate and easy to learn, yet it produces early postoperative discomfort and scar formation by tie - over dressing. The authors modified the Inaba's procedure by using delayed suture of the incision wound and omitting tie - over dressing. The comparative study of Inaba's procedure and its modification was performed to confirm the advantages of modified procedure. Methods: The study contains the retrospective analysis of the medical records of 296 patients with osmidrosis who were treated using the Inaba's procedure from December, 1996 to February, 2007. The study also contains the prospective analysis of 20 patients, from March, 2007 to July, 2008, who were treated by the modified Inaba's procedure with delayed suture of the incision wound and gentle pressure dressing instead of tie - over dressing. The operative results of two groups were compared and verified by Mann - Whitney U test(SPSS 12.0). Results: The incidence of complications was 14.5% in the Inaba's procedure, whereas 6.2% in the modified Inaba's procedure. Both procedures have the same basic surgical procedure in terms of the location of incision site and subdermal shaving of the sweat glands, and therefore similar good results were obtained in the aspect of postoperative axillary odor, recurrent rate and postoperative condition of axillary hair. Certainly, the modified Inaba's procedure had better outcome in each element of PSS(Patient Scar Self-Rating Scale), compared to the Inaba's procedure. In addition, the modified Inaba's procedure showed a statistical significance in dressing - related pain reduction and overall satisfaction. Conclusion: The modified Inaba's procedure had advantages of decreased early postoperative complications such as hematoma, discomfort and pain caused by tie - over dressing, and decreased scar formation. However, the drawback was delayed suture of the incision wound after 48 hours.
The purpose of this study were to make clear the actual state of indoor air quality by cooling in private institute classrooms and to analyze influencing factors on indoor air quality. The measurements of physical elements and observations of architectural characteristics and living conditions were carried out in each 1 classroom of 5 private institutes. Measuring elements were indoor temperature, relative humidity, $PM_{10}$, HCHO, CO, and $CO_2$ concentration. As results, the averages of indoor temperature each classroom were $21.7\sim28.1^{\circ}C$. 3 classrooms were lower than the Maintenance standard($26\sim28^{\circ}C$) of School Health Law. The means of relative humidity were 48$\sim$63%, all classrooms were ranged within the standard(30$\sim$80%). The means of $PM_{10}$ concentration were 2.1 $\sim$ 17 $\mu g/m^3$, all classrooms were kept within the standard(100 $\mu g/m^3$). The means of HCHO concentration were 0.02$\sim$0.16 ppm, 2 classrooms were exceed the standard(0.1 ppm). The means of CO concentration were 1.4$\sim$3.6 ppm, all classrooms were kept within the standard(10ppm). The means of $CO_2$ concentration were 1,593$\sim$3,819 ppm, all classrooms were exceed the standard(1,000ppm). The results of analysis on influencing factors of the physical elements are as follow; the air conditioner set of temperature, personal necessities like hair spray, teacher's smoking in corridor, windows and doors opening time, the number of students, and volume of classroom.
Kim, Siwon;Lee, Minji;Yoon, Dahye;Lee, Dong-Kye;Choi, Hye-Jin;Kim, Suhkmann
Bulletin of the Korean Chemical Society
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v.34
no.8
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pp.2413-2418
/
2013
Forensic and legal medicine require reliable data to indicate excessive alcohol consumption. Ethanol is oxidatively metabolized to acetate by alcohol dehydrogenase and non-oxidatively metabolized to ethyl glucuronide (EtG), ethyl sulfate (EtS), phosphatidylethanol, or fatty acid ethyl esters (FAEE). Oxidative metabolism is too rapid to provide biomarkers for the detection of ethanol ingestion. However, the non-oxidative metabolite EtG is a useful biomarker because it is stable, non-volatile, water soluble, highly sensitive, and is detected in body fluid, hair, and tissues. EtG analysis methods such as mass spectroscopy, chromatography, or enzyme-linked immunosorbent assay techniques are currently in use. We suggest that nuclear magnetic resonance (NMR) spectroscopy could be used to monitor ethanol intake. As with current conventional methods, NMR spectroscopy doesn't require complicated pretreatments or sample separation. This method has the advantages of short acquisition time, simple sample preparation, reproducibility, and accuracy. In addition, all proton-containing compounds can be detected. In this study, we performed $^1H$ NMR analyses of urine to monitor the ethanol and EtG. Urinary samples were collected over time from 5 male volunteers. We confirmed that ethanol and EtG signals could be detected with NMR spectroscopy. Ethanol signals increased immediately upon alcohol intake, but decreased sharply over time. In contrast, EtG signal increased and reached a maximum about 9 h later, after which the EtG signal decreased gradually and remained detectable after 20-25 h. Based on these results, we suggest that $^1H$ NMR spectroscopy may be used to identify ethanol non-oxidative metabolites without the need for sample pretreatment.
Journal of the Society of Cosmetic Scientists of Korea
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v.19
no.1
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pp.1-19
/
1993
Gas chromatography serves to separate the mixtures into its components, and mass spectrometer is used to analyzing of unknown compounds. But there are many problems the identification of horn compounds using by GC only. As this reason GC/MS a very powerful analyzing technique. Mass spectrometer consists of 1) inlet stem 2) ion source 3) Bass filter 4) detectors and 5) data system. There are two analyzing modes in the GC/MS, those are scan and SIM(selected ion mom toping) modes. Scan mode is used when analyzing unknown compounds and SIM mode al lows the mass spectrometer to detect specific compound with very high sensitivity. As GC/MS applications for the analysis of cosmetic products, volatile compounds in lotion, earn foundation and hair color, and carbon distribution of fatty acids in soap were performed. Also as a new sample pre-treatment technique, head space sampler/GC/MS introduced in order to analyze the volatile compounds in a toothpaste.
The purpose of this study was to investigate the appearance management behaviors and motives differentiated by the body image of college men. A questionnaire was designed for the survey and the subjects were 228 college in Pusan and the Kyongnam province, Korea. The statistical analyses were carried out in the forms of frequency, factor analysis, cluster analysis, ANOVA and Duncan's multiple range test. The findings throughout the research are as follows; First, according to the multidimensional body image of the subjects, the college men were classified into three groups, i.e. the appearance concerning and satisfied group, the weight concerning group, and the appearance indifferent group. The appearance management behaviors of the college men consist of innovative appearance management, weight reduction, appearance management through apparel and fashion products, body shape care, skin care, hair care and health care. Second, the weight concerning group showed a higher BMI than those of the other groups. The means of the monthly income and the expenses for the appearance management of the appearance indifferent group were lower than those of the other groups. Third, the college men were generally shown to pursue the motive improving sociality. The appearance concerning and satisfied group and the weight concerning group indicated higher pursuing motives than the appearance indifferent group in the appearance management motives. Finally, there were significant differences in the appearance management behaviors among the three groups. While the weight concerning group showed the more concerning appearance management behaviors, the appearance indifferent group had the least appearance concerning tendency.
We have utilized the PCR-RFLP method to detect the ryanodine receptor(RYR1) gene mutation and to estimate the genotype frequencies of the RYR1 gene in commercial crossbred pig population. The exon region(659bp) including point mutation(C ${\rightarrow}$T; Arg ${\rightarrow}$Cys) in the porcine ryanodine receptor gene, which is a causal mutation for PSS, was amplified by PCR and digested with Cfo I restriction enzyme. The RYR1 gene was classified into three genotypes by agarose gel electrophoresis. The normal homozygous(NN) individuals showed two DNA fragments consisted of 493 and 166bp. The mutant homozygous(nn) individuals showed only one DNA fragment of 659bp. Also, all three fragments(659, 493 and 166bp) were showed in heterozygous(Nn) carrier animals. The proportions of normal, carrier and PSS pigs within crossbred population of pigs were 81%, 15% and 4%, respectively. According to the results of analysis of variance for the association of genotypes of RYR1 of pigs at 30kg, day age at 90kg and average daily gains, the RYR1 nn genotype was very higher than RYR1 NN genotype for day age at 30kg with 5% level of significant difference, but no significant difference for association of any other genotypes with day age at 90kg and average daily gain in crossbred pigs. Therefore, DNA diagnosis by using PCR-RFLP analysis for the PSS gene was useful for large-scale screening of commercial pigs in the swine industry.
Objectives The purpose of this study is to investigate whether Korean oriental pediatrics hospitals are using their feedback surveys to improve their medical conditions. Methods We selected 24 oriental medicine university hospitals and sent surveys to investigate. 15 of those surveys were analyzed. Results The results were classified as follows; Only 9 of university hospital said that they are currently using their feedback surveys to improve their services. Question for asking the purpose of doing their feedback surveys, 58.3% of hospital responded to get some information for diagnosis, 25% of them said to see the progress of treatment of specific disease, and 16.7% answered for using their data to the further studies. The question about the reason why hospital were need to make these feedback surveys, 46.2% of them answered to apply better service, 38.4% of them said to post medical journal. The question for asking what do oriental medical hospitals check from feedback surveys, 40% of them said they check Ryodoraku, 33.3% was pulse diagnostic apparatus, EAV(Electro-puncture According to Voll) was 26.7%. The classification according to purpose of test resulted as follows; information for diagnosis was 48.1%, the progress of a disease was 37.3%. In addition oriental medical test there were blood test, radiological test, brain waves test, Hair Tissue Mineral Analysis(HTMA), ultrasonic test, body composition analysis. Conclusions Further clinical research is necessary for improvement of using the feedback survey effectively.
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