• Title/Summary/Keyword: Haeyeol-tang

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The Inhibitory Effects of Haeyeol-tang in Expression of Pro-inflammatory Cytokine on LPS-stimulated THP-1 Cells (해열탕(解熱湯)이 LPS로 자극된 대식세포에 있어 염증관련 Cytokine 발현억제에 미치는 효과)

  • Lee, Byeong-Sam;Kim, Hong-yeoul;Kim, Jin-Ju;Jung, Sung-Ki;Rhee, Hyung-Koo;Jung, Hee-Jae
    • The Journal of Internal Korean Medicine
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    • v.29 no.2
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    • pp.334-347
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    • 2008
  • Background and Objective : Haeyeol-tang, composed of Houttuyniae Herba, Lonicerae Flos, Taraxaci Herba, and Scrophulariae Radix, is widely used for alleviating the symptom of various kinds of inflammatory pulmonary disease, including asthma and COPD. We want to know whether Haeyeol-tang has an anti-inflammatory effect by analyzing expression of pro-inflammatory cytokines. Materials and Methods : We differentiated the THP-1 cells into macrophage-like cells by treatment with PMA. Inflammation was induced by treatment with LPS and PMA. We found the safe concentration of Haeyeol-tang by using MTS assay and used PD98059 as a negative control for comparison of anti-inflammatory effect of Haeyeol-tang. Results : The RT-PCR analysis results show that the cell survival rate is over 100% within 1 ng/mL to 1 ug/mL of Haeyeol-tang and begins to decrease under 100% at 10 ug/mL. The gene expression of $IL-1{\beta}$, IL-6, IL-8, IL-10, $TNF-{\alpha}$ and $TGF-{\beta}$ levels were down-regulated when Haeyeol-tang was treated at concentrations between 1 ng/mL an 1 ug/mL on monocyte-derived macrophages. Interestingly, 1 ug/mL Haeyeol-tang-treated samples showed that the transcriptional activities of IL-8, $TNF-{\alpha}$, IL-10 and $TGF-{\beta}$ were more down-regulated than those of PD098059 $(TNF-{\alpha}$ inhibitor). At protein level, the ELISA analysis results showed that there were more remarkable (p<0.001) decreases in expression of $IL-1{\beta}$, IL-6, IL-8 and $TNF-{\alpha}$ on both the 1 ug/mL Haeyeol-tang-treated group and the PD98059-treated group than the LPS-treated group. Conclusion : We conclude that Haeyeol-tang has an anti-inflammatory effect by inhibiting expression of pro-inflammatory cytokines and chemokines at mRNA and protein levels. These results may provide us a promising way to care for general inflammatory diseases as well as inflammatory pulmonary disease, including asthma and COPD, with further clinical study.

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Experimental Research on the Effect of Haeyeol-tang (해열탕(解熱湯)의 항염증(抗炎症), 항산화(抗酸化), 항노화(抗老化) 작용(作用)에 대한 실험적(實驗的) 연구(硏究))

  • Chu, Cheng-Kang;Kim, Jin-Ju;Roh, Young-Lae;Lee, Sung-Hun;Jeong, Seung-Yeon;Jung, Hee-Jae;Jung, Sung-Ki
    • The Journal of Internal Korean Medicine
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    • v.28 no.4
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    • pp.671-680
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    • 2007
  • Objectives : The purpose of this study was to examine the effect of Haeyeol-tang on infectious disease of respiratory system. Methods : Haeyeol-tang was used by cold storage after dilution with 1, 10 and $100{\mu}g/ml$ of PBS, from dried material as extracted material with extraction with 50% ethanol. Statistical significance was examined through measuring TNF-$\alpha$, RT-PCR, DPPH, nitric oxide(NO), and MTT of Raw264.7 cells activated with LPS. Results : For the toxicity experiment of Haeyeol-tang, almost no toxicity was shown on cells in the concentration ranges of 1, 10 and $100{\mu}g/ml$. For the effect of NO production, Haeyeol-tang showed significant (p<0.001) decrease of NO production, according to its concentration. For the effect on the eliminating activity of DPPH radical, Haeyeol-tang showed increasing tendency of eliminating activity of DPPH radical, according to its concentration. For the effect on revelation of NOS2 enzyme, Haeyeol-tang showed inhibiting tendency against the revelation of NOS2, according to its concentration. For the effect on the TNF-$\alpha$ production, Haeyeol-tang showed significant (p<0.001) decrease of TNF-$\alpha$ production. Conclusions : This result means that Haeyeol-tang is effective for anti-inflammation, as well as excellent effect for anti-oxidation and anti-aging.

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A Case Report of a Patient Diagnosed with Nasal Polyp Treated with Korean Medicine (한약 치료를 통해 비용종(Nasal polyp)이 소실된 부비동염 환자 치험 증례 1례)

  • Yu, Chang-hwan;Do, Ha-yoon;Kang, Sung-woo;Roh, Ji-ae;Kim, Kwan-il;Lee, Beom-joon;Jung, Hee-jae
    • The Journal of Internal Korean Medicine
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    • v.40 no.5
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    • pp.752-759
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    • 2019
  • Objective: The purpose of this case study was to report the effect of Korean medicine treatment on a patient diagnosed with a nasal polyp. Methods: A 62-year-old female patient with a nasal polyp visited the clinic sixteen times from September 11th, 2018 to December 18th, 2018. The patient was treated with Eunhwayeongyo-tang, Haeyeol-tang, Hongsamkyungok-go extract, and acupuncture during the treatment period. We evaluated the improvement of symptoms by nasoendoscopy. Results: After the Korean medicine treatment, the nasal polyp was completely eradicated. Hyperemia of the nasal mucous membrane and rhinorrhea were also relieved. Conclusion: The study findings suggested that Korean medicine treatment (Eunhwayeongyo-tang, Haeyeol-tang, and Hongsamkyungok-go extract, and acupuncture) might be effective for treatment of patients diagnosed with nasal polyps.

A Case Report of Symptom Improvement in a Patient Diagnosed with Nontuberculous Mycobacterial Lung Disease Treated with Korean Medicine (비결핵 항산균 폐질환 환자의 한의치료로 증상 호전에 대한 증례보고)

  • Kang, Sung-woo;Yu, Chang-hwan;Hong, Sung-eun;Kim, Dae-young;Kim, Kwan-il;Lee, Beom-joon;Jung, Hee-jae
    • The Journal of Internal Korean Medicine
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    • v.41 no.5
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    • pp.856-867
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    • 2020
  • Objectives: The purpose of this case study was to report the effect of Korean Medicine treatment on a patient diagnosed with nontuberculous mycobacterial lung disease. Methods: A 61-year-old female patient with nontuberculous mycobacterial lung disease was admitted to the clinic from October 15th, 2018 to November 7th, 2018. The patient was treated using Korean medical treatments of Jinhae-tang-gami, Ssanghwa-tang-gami, Haeyeol-tang, Jinhae-tang plus Ssanghwa-tang-gami, and Jinhae-tang plus Bojungikgi-tang-gami. We evaluated the improvement of symptoms by a numeric rating scale (NRS) score, C-reactive protein (CRP) levels, erythrocyte sedimentation rate (ESR), and body temperature. Results: After Korean medicine treatment, the patient's cough and sputum amounts were decreased as measured by the NRS score. CRP levels and ESR were also decreased and fever was relieved. Conclusion: This study suggested that Korean medicine treatment might be effective in treating patients diagnosed with nontuberculous mycobacterial lung disease.

Two Case Reports of Pneumonia in Elderly Patients That Was Improved by Traditional Korean Medicine Treatment (노인성 폐렴 한방 치험 2례)

  • Bhang, Yeon-hee;Baek, Hyun-jung;Kim, Jae-hyo;Kim, Sang-jin;Kim, Kwan-il;Lee, Beom-joon;Jung, Sung-ki;Jung, Hee-jae
    • The Journal of Internal Korean Medicine
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    • v.37 no.2
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    • pp.352-360
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    • 2016
  • Objective: This study reports on traditional Korean medicine therapy used for pneumonia among elderly patients.Method: Two patients diagnosed with pneumonia were treated with herbal medicine and acupuncture, as well as cupping along the back. We checked chest X-rays, coughing and sputum on the visual analogue scale (VAS), and lab evaluations in order to evaluate the effectiveness of the treatment.Results: Following treatment with traditional Korean medicine therapy, VAS scores for coughing and sputum decreased and the chest X-rays and inflammation markers improved.Conclusion: Traditional Korean medicine therapy treatment appears to be effective for treating pneumonia in elderly patients.