• Journal of Life Science
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• v.15 no.4 s.71
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• pp.566-571
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• 2005

A natural habitat bacterium, Enterobacter cloaceae K41 was isolated from fresh water plant root and identified. This strain was used to investigate heavy metal resistance. The optimal growth conditions of the bacterium were LB medium containing$1\%$ yeast extract, $1\%$ lactose, $1\%$ NaCl, pH 7.0, at $37^{\circ}C$, and for 24 hours on a shaker. The minimal inhibitory concentration (MIC) of heavy metals against E. cloaceae KCTC2519 and E. cloaceae K41 was compared. The MIC of E. cloaceae K41 was 150 ppm in Cu, 50 ppm in Cd whereas that of the standard strain was 50 ppm in Cu but no growth was observed either Cd or two mixed heavy metal solution. The presence of plasmid was cleared from the isolated strain whereas no possession from the standard strain. The plasmid from E. cloaceae K41 was transformed into E. coli $DH5{\alpha}$. The MIC of transformed strain increased resistance 7 times in Cu and 6 times in Cd by insertion of this plasmid. The metal adsorption of the transformant was increased 1.3 times in Cu and 1.5 times in Cd indicating the plasmid was responsible for heavy metal resistance.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.17 no.1
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• pp.81-90
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• 2014

A Fairy Pitta is a bird known to breed only in mainland China, Taiwan, Japan and Korea and is listed as Vulnerable in the IUCN Red List. We carried out a DNA analysis to contribute to conserve the genetic diversity of Fairy Pitta. 32 samples were collected at Jeju Island, the Korean Peninsula and Taiwan from 2004 to 2013 and DNA was extracted from them and several sequences were amplified-it through PCR. And then we performed the population genetic analysis. We found there was a transversion between nucleotide sequences at CO1 gene, while there was no changes at Cyt-b gene. And we confirmed the polymorphism from two genes was caused from genetic drift not from selection. Through this analysis, the group within the Peninsula was found bigger than other two groups based on the analysis of CO1 gene, and the group from Taiwan was found bigger than other two groups through the analysis of Cyt-b gene. The population genetic structure of mitochondria gene of three group was showing CO1 gene had 5 haplotypes and Cyt-b gene had 6 haplotypes. Haplotype 2 in CO1 gene was found in three group and many individuals of samples had this haplotype. Like CO1 gene, haplotype 2 in Cyt-b gene was found in three group and was included in plenty of individuals. Other haplotypes were not overlaped and broke off among the three groups. To prevent from the extinction of Fairy Pitta and to obtain the genetic diversity, we need to compare with other regional group such as Japan, China and perform additional research in the non-breeding area.

• Korean Journal of Environmental Agriculture
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• v.27 no.1
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• pp.72-79
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• 2008

Phytosociological study was carried out to investigate the structural characteristics of Humulus japonicus community in the Middle area of Korea. The vegetation data of total 67 $relev\acute{e}s$ were analyzed by the $Z\ddot{u}rich$-Montpellier school's method. Upper layer plant communities of Humulus japonicus were classified into four community groups : Humulus japonicus-Artemisia princeps community, Chenopodium album community, Setaria viridis-Echinochloa crus-galli community and Typical community. Lower layer plant communities were classified into five community groups : Galium spurium-Stellaria aquatica community, Equisetum arvense community, Persicaria theunbergii community, Echinochloa crus-galli-Digitaria ciliaris community and Typical community. According to the results of canonical correspondence analysis by vegetation and environmental factor, Setaria viridis-Echinochloa crus-galli community of upper layer was distributed throughout the soil with high relative light and soil hardness. Chenopodium album community appeared in the soil with high pH. Galium spurium-Stellaria aquatica community of lower layer was formed in the high soil pH. Persicaria theunbergii community and Echinochloa crus-galli-Digitaria ciliaris community were distributed in the soil with high moisture.

• Korean Journal of Environmental Biology
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• v.32 no.1
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• pp.49-57
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• 2014

Orb-web spiders can be considered as an effective bio-indicative animal to monitor the ecological air pollution of certain habitat since they have limited shifting ability during their life spans. In this study we revealed the fine structural characteristics of booklung in the orb-web spider Nephila clavata, and its bio-indicative significance in the monitoring environmental asbestos exposure. The high resolution scanning electron microscopy results suggest that the cuticular spikes near the atrium could be act as an effective filtering structure for dust materials. Furthermore, inner cuticular spikes which protruded across the air sacs, have the functions not only sustaining appropriate respiratory volume of each air sac but also help to enhance respiratory capacity by maximizing the gross surface area for gas exchange. Interestingly, in spite of short exposure, the asbestose fibrils were effectively captured on the surface of the atrial cuticular spikes. Furthermore, histologic damages were observed due to penetration of asbestos fibrils through air sacs and fixed on the hemolymph space. In addition, although accumulated mass of hemocytes were observed near the fibrils, there were no asbestos bodies or coagulated hemocytes were found on the surface of the fibrils, This could be mainly due to the short exposure period towards asbestos. Briefly, these results indicate the spider's booklung could be valuable tool in the detection ecological air pollutants.

• The Korean Journal of Mycology
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• v.38 no.2
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• pp.95-98
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• 2010

One interesting parasitic forming fungus, being grown on the eggs of Phallus impudicus was collected from Mulchajoreum and on woods of Saryeoni in Jeju island. This taxon identified as a Podostroma solmsii (E. Fisch.) S. Imai f. octospora Yoshim. Doi (Hyporeaceae) based on morphological characteristics. According to the interpretation of Doi(1978), this taxon hsving the stipitate, genus Podostroma, family Hypocreaceae, Hypocreales, Ascomycota was identified as Podostroma solmsi f. octospora and its korean common name designated by authors. This species is characterized in having the fusiforme to ellipsoidal, unicellular and warted ascospores without septate and with blunt apiculus at each end and mycoparasitic habitat. Although there is some mycologists suggest that this taxon having above characters needs to be derived from Hypomyces. But authors are primarily going to record this species as a named "Podostroma solmsii (E. Fisch.) S. Imai f. octospora Yoshim. Doi" for the fungus flora of Korea. In a future, it is need in detail to compare their DNA sequencing data on the type species and some related species. All collections cited here are deposited in the Herbarium Conservation Center of National Academy of Agricultural Sciences.

• Journal of Korea Water Resources Association
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• v.44 no.1
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• pp.23-30
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• 2011

Any significant inflow of fresh water due to flood or snow melting can rapidly reduce salinity at the bay. In such a case, the habitat environment in the sea near river mouth can be partly destroyed. Therefore, research to understand the salinity distribution quantitatively at the bay for the utilization of natural environment and for the inhabitant conservation must be very important. In this study, the investigation on the relationship between satellite image and turbidity is carried out first, and then the salinity distribution at the bay using the relationship between turbidity and salinity is derived. The main results are as follows. First the reappearance ability of RGB bands respectively of the satellite image is investigated, and then it was confirmed that the combination of band2 and band3 expressed best the movement characteristics of turbid water at the bay is chopped up into 4 small areas. Second the turbidity of river mouth is estimated using the travel time from the upward monitoring station to the river mouth. Finally the satellite image is converted into the salinity distribution by the correlation of salinity and turbidity. It is confirmed that the salinity distributions obtained from above three investigation methods are quite reasonable and clear.

• Journal of Korea Water Resources Association
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• v.51 no.1
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• pp.35-48
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• 2018

Groynes are installed generally to protect the riverside or the river bank from the erosion caused by water flows by controlling the flow direction and velocity in rivers. In the past, groynes were used to secure enough depth of water in canals. As there has been a growing interest in river restoration and the natural river maintenance since 2000, groynes are proposed as a major environmental hydraulic structure because the flow control and various river bed conditions around the groyne can contribute to habitat functions. Groynes are typically installed in a series. In designing groyne series, groyne spacing is an important factor because the flow changes in the main canal and the flow inside the groyne area occurs variably depending on the groyne spacing. This study provide information to determine the groyne spacing suitable for the purpose of the groyne by examining the flows that variably changes according to the groyne spacing and angle in the recirculation zone of the groyne field. In particular, the formation of vortex, the location of vortex core and the water flow near the river bank, all of which occur in the recirculation zone inside the groyne area, were mainly analyzed to examine the flow characteristics near the river bank that influences the safety of the river bank area. The results of the experiment will serve as important basic data to examine changes in the river bed inside the groyne area as well as the safety of river banks following the installation of groyne series.

• Korean Journal of Ecology and Environment
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• v.47 no.spc
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• pp.10-18
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• 2014

The habitat characteristic of the Gammarus sobaegensis which is one of the Amphipoda kinds has been found inhabiting in the inter mountain stream at Ganseong stream. From the investigation, the main inhabitant area of the G. sobaegensis maintained the water temperature under $10^{\circ}C$ during the spring and autumn season, and the electric conductivity was also maintaining under $40{\mu}Scm^{-1}$. For the precipitation, for this year when the monthly accredited rainfall did not exceed 800 mm, it did not affect maintaining the population of the G. sobaegensis but when the monthly accredited rainfall exceeded 1,000 mm it was found to be working as a unfavorable condition for maintaining the population. The effect especially from this kind of rainfall created the flow of G. sobaegensis as well as the flow of the fallen leaves which are the food resources and affected the population of the G. sobaegensis as a multiple interruption reason. Among the Functional Feeding Groups (FFGs) distribution depending on the use of the food resources the shredders showed up in the ratio of 20% in the site 1~3, and showed a big difference with the site 4~7 as it showed up in the ratio near 10%. Also, after comparing the variation of the individual number among the G. sobaegensis and shredders at the site 1~3 where G. sobaegensis mainly shows up, it showed the same variation pattern except for 2009.

• Korean Journal of Environmental Biology
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• v.35 no.4
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• pp.437-445
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• 2017

An analysis of recent studies and a field survey were conducted to investigate the distribution of the exotic species Ophraella communa LeSage (Coleoptera, Chrysomelidae) and the results' applicability for biological control against ragweed, which disturbs the island's ecosystem. The ragweed beetle (O. communa) can be found anywhere on the host plant Ambrosia artemisiifolia L., which grows in Jeju Island. Moreover, ragweed beetles possess the following characteristics: a high host plant specificity, a high-temperature resistance, an excellent mobility, and multivoltinism. Ragweed is extensively distributed and gradually expands its habitat further on Jeju Island. For this reason, it is recommended to take advantage of O. communa for the biological control of ragweed instead of pesticides or direct removal, considering that Jeju is endowed with many natural reserves and unique insular traits.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.32 no.4
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• pp.525-533
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• 1999

In the Korea Strait total of 96 copepod taxa (40 genera) were identified from the seasonal and vertically stratified samples. Species richness was the highest in fall and the abundance was the highest in spring. Spatial differences were not significant within each season, but was meaningful among seasons. The water column layers of high abundances were near bottom in spring, and surface in summer and fall. Species association of copepods was examined by the cluster analysis. There are the two different results on the timing of the intrusion of East Sea Cold Water to the Korea Strait in the deeper layer based on physical data [in winter (Lee et al., 1938) vs in summer (Cho and Kim (1998)]. This study based on the distributional characteristics of copepods supported Cho and Kim (1998)'s result. Although sea water temperatures was one of major controlling factors of the copepod distribution in this strait, biological interrelation among the species such as common or exclusive exploitation of the habitat was also responsible for the observed distributional patterns.