• YAKHAK HOEJI
• /
• v.36 no.6
• /
• pp.529-537
• /
• 1992

To investigate the effect of ursolic acid on the expression of oncogenes in tumor cells of mice, sarcoma 180 ascites tumor cells were implanted into the left groin of ICR mice and the tumor bearing mice were treated with ursolic acid. The expression of oncogenes were measured by in situ hybridization method. Ursolic acid significantly reduced the expression of oncogenes in the tumor cells. Therefore, it can be said that the prestated anticarcinogenic effect of ursolic acid could be partly ascribed to the mechanism included in the oncogene´s transcription level.

• Proceedings of the Korean Society of Food Hygiene and Safety Conference
• /
• 2001.10a
• /
• pp.71-73
• /
• 2001

• Korean Journal of Animal Reproduction
• /
• v.17 no.1
• /
• pp.13-19
• /
• 1993

We investigated the effects of CP-2 extracted from the mixture of Copis and Croton tiglium L, which showed very high cytotoxic effect to the various tumor cells, on the growth and steroidenesis of primary and transformed cell lines PA-GS6 and PO-GRS1 by cotransfectionwith SV40 and Ha ras oncogenes. CP-2 inhibited the growth of PA-GS6 and PO-GRS1 in a dose dependent manner when the growth of them was measured by cell number and by protein content, while CP-2 did not affect the growth of primary granulose cells. Productions of progesterone ofprimary and transformed granulosa cells were stimulated by forskolin, but this stimulatory effect was blocked by treatment of CP-2. Clinical application of CP-2 asa new anti-cancer drug and utilization of transformed granulosa cells as a model system for the screening of anti-cancer drug were also discussed.

• 한국방재학회:학술대회논문집
• /
• 2010.02a
• /
• pp.95.2-95.2
• /
• 2010

조도계수는 자연하천의 흐름해석에 사용되는 매우 중요한 변수로서, 하천의 단면, 하상입자들의 크기 및 형상, 식생, 수로단면의 변화, 수로의 만곡, 수위와 유량 등 매우 복합적인 요소의 영향을 받는 경험적 매개변수이다. 일반 자연하천에서는 유량이 적어질 경우 하천구간 내 여울이나 보의 영향으로 수면 불연속 흐름이 발생할 가능성이 커지기 때문에 수리학적 모형을 이용하여 조도계수를 산정할 경우 계산 구간 내 수면 불연속 구간에서 부정확한 조도계수가 산정되는 한계가 있다. 따라서 본 연구에서는 여울이나 장애물이 존재하지 않는 대표하천을 선정하여 대표적인 특성을 갖는 하천에 대하여 평 갈수기 조도계수를 산정하였다.

• Proceedings of the Korean Society of Toxicology Conference
• /
• 1998.10a
• /
• pp.36-36
• /
• 1998

Tumorigenicity of benzo(a)pyrene (BP) and benzo(a)pyrene diol epoxides ((+)BPDE-1, (-)BPDE-1) was investigated in transgenic TG-AC mice carrying v-Ha-ras oncogene fused to the promoter of the mouse embryonic a-like, z-globin gene. Animals were topically treated twice per week for 25weeks with BPDE (10$\mu$g/mouse) and BP (10, 20, 40$\mu$g/mouse). In addition, animals were treated with BPDE or BP (initiated) followed by TPA (2$\times$2.5$\mu$g/week, for 4 weeks) for promotion study. In the continuous treatment of BPDE or BP, animals treated with 40$\mu$g BP showed $100\%$ tumor response after 20 weeks, $40\%$ of mice for 20$\mu$g BP, and $20\%$ for (+)BPDE-1, but (-)BPDE-1 and 10$\mu$g BP did not show any tumor response. After 25 weeks, most tumors turned out to be carcinomas in animals treated with 40$\mu$g BP. In BPDE or BP/TPA Initiation-promotion study, papilloma response occurred earlier (6 weeks after TPA treatment) than in continuously treated animals with BPDE or BP. RT-PCR assay for transgene expression showed that BP or BPOE was not transgene dependent in its tumorigenicity, but TPA was. Several Cytokine genes(TGF-a, TNF-a) and c-myc gene expressions were monitored in skin tissues during BP carcinogenesis. In early stage of BP treatment, the gene expressions were elevated(c-myc,TGF-a) or unchanged(TNF-a) compared to control, but the levels were gradually decreased during both middle and late stages of cacinogenesis, Gene expression levels of skin papillomas in acetone initiated-TPA promoted animals were close to those of middle stage or between middle and late stages. i-NOS was also highly expressed in carcinoma and papilloma, These data suggest that transgene expressions of TG-AC mice were not dependent on BP carcinogenesis and that TG-AC mice were more sensitive to TPA regardless of types of initiators. In addition, genes(TGF-a, c-myc, TNF-a, i-NOS) were modulated in the skin during BP cacinogenesis or TPA promotion.

• Environmental Mutagens and Carcinogens
• /
• v.26 no.3
• /
• pp.77-85
• /
• 2006

2,3,7,8-Tetrachlorodibenzo-$\rho$-dioxin(TCDD) displays high toxicity in animals and has been implicated in human carcinogenesis. Although TCDD is recognized as potent carcinogens, relatively little is known about their role in the tumor promotion and carcinogenesis. It is known that TCDD can increase of cancer risk from various types of tissue by a mechanism possibly involving the aryl hydrocarbon receptor (AhR) activation. In this study, effects of TCDD on cellular proliferation of normal human skin and lung fibroblasts, Detroit551 and WI38 cells were investigated. In addition, to enhance our understanding of TCDD-mediated carcinogenesis, we have investigated process in which expression of Erk1/2, cyclinD1, oncogene such as Ha-ras and c-myc, and their cognate signaling pathway. TCDD that are potent activators of AhR-mediated activity was found to induce significant increase of cytochrome P4501A1 mRNA expression, suggesting a presence of functional AhR. These results support that CYP1A1 enzyme may be involved in the generation of TCDD-induced toxicity. Moreover mitogen-activated protein kinases (MARKs) phosphorylation and cyclin D1 overexpression are induced by TCDD, which corresponded with the progression of cellular proliferation. However, TCDD did not affected Ha-ras and c-myc mRNA expression. Taken together, it seems that TCDD are could be a part of cellular proliferation in non-tumorigenic normal human cells such as Detroit551 and WI38 cells through the upregulation of MAPKs signaling pathway regulating growth of cell population. Therefore, AhR-activating TCDD could potentially contribute to tumor promotion and Detroit551 and WI38 cells have been used as a detection system of tumorigenic effects of TCDD.

• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.10 no.1
• /
• pp.23-35
• /
• 2007

Nowadays, as a result of increasing income and spare time, the social demands for better living environment become more increasing. Above all, landscape improvement, the essential part of physical environment, will be a more important subject in urban planning. In these circumstances, classification of urban scenic quality is required for urban landscape development programs. The major purpose of this study is to suggest a technical method of designing urban scenic quality map for urban district area based on the scenery management system of the USDA forest service and literature studies. As a conclusion of this study, four steps are desirable for the technical method for designing a scenic quality map of urban districts areas : 1) Define a landscape unit on the map, 2) Take a photograph of these landscape unit on site, 3) Evaluate the landscape unit by semantic differential scale with landscape adjectives, and 4) Draw the scenic quality map, investigate the landscape characteristics and suggest the landscape scenic development plans.

• Asian Pacific Journal of Cancer Prevention
• /
• v.14 no.12
• /
• pp.7713-7718
• /
• 2013

DNA methylation is considered a promising biomarkers for diagnosis of cancer in general and of ovarian cancer in particular. In our study, we validated the accuracy of methylation specific polymerase chain reaction (MSP) to analyze the methylation pattern of BRCA1, RASSF1A and ER in 59 and 10 Vietnamese patients with epithelial ovarian cancer (EOC) and benign ovarian tumors, respectively. We found methylation of BRCA1, RASSF1A and ER in 11/59 (18.6%), 40/59 (67.8%) and 15/59 (25.4%) of EOC cases, while methylation of BRCA1 was only detected in 2/10 (20%) benign ovarian patients. Forty five out of the 59 EOCs (78%) demonstrated methylation at one or more genes. The methylation frequency of RASSF1A was significantly associated with EOC (p<0.0005). No significant association was observed between methylation status of these genes and the clinical and pathological parameters of tumors collected from Vietnamese women suffering from ovarian cancer.

• Journal of the Korean Institute of Landscape Architecture
• /
• v.33 no.4 s.111
• /
• pp.1-10
• /
• 2005

Recently, as urban landscape is growing in importance, urban landscape planning is being actively performed. for this purpose, classification of the urban landscape is definitely required. Therefore, this research focuses on classifying urban landscape in Daejeon metropolis by dividing the urban land use pattern. This results are as follows. 1. Urban land use pattern is divided into 20 classes. The residential, commercial and industrial areas, the old market and the bus terminal are evaluated negatively, whereas the areas of school, water reservoir, neighborhood park and train station are appreciated as being positive in landscape characters. 2. As a result of a cluster analysis, urban landscape has five different landscape types. These are: landscapes of medium diversity lacking green area, landscapes of high diversity lacking green area, landscapes rich in green area and with medium diversity, landscapes rich in green area and with high diversity, and landscapes rich in green area and with low diversity. 3. In landscape characters of beauty and harmony, landscapes rich in green area and with medium diversity are more positively evaluated than those rich in green area and with low diversity. This point should be taken into account for planning the urban landscape.

• BMB Reports
• /
• v.43 no.3
• /
• pp.205-211
• /
• 2010

Sprouty (Spry) proteins have previously been suggested as negative regulators of the MAPK pathway through interaction with Raf-1. However, the molecular basis of this inhibition has not been elucidated. In this study, we used cells expressing FLAGtagged Raf-1 with point mutations at known phosphorylation sites to reveal that activation of Raf-1 mutants does not correlate with their degree of interaction with Spry2. The association of Raf-1 with Spry2 in intact cells was further corroborated by immunofluorescence colocalization. Additionally, there was no significant change observed in the strength of interaction between Raf-1 mutants and Spry2 after paclitaxel treatment despite differences in the activation levels of these mutants. Thus, our study provides the evidence that Spry2 does not directly regulate Raf-1 kinase activity, but instead acts as a scaffolding protein that assists interactions between Raf-1 kinase and its direct regulators.