• Proceedings of the Plant Resources Society of Korea Conference
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• 2023.04a
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• pp.26-26
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• 2023

포스트 코로나 이후 먹거리에 대한 소비자의 인식전환으로 건강먹거리인 야콘에 대한 관심이 증가하고 있다. 야콘은 남미 안데스에서 도입된지 40여년 되었으며 달콤한 맛과 아삭아삭한 식감을 가져 생식용과 야콘즙으로 인기있으나 현재 국내 육종된 품종이 없다. 따라서, 다양한 유전자원을 확보하고 자원에 대한 평가를 통해 신품종 육성을 위한 기초 자료로 활용하고자 실시하였다. 연구에 사용된 재료는 국립식량과학원에서 보유하고 있는 5개 자원을 대상으로 초장, 엽수, 줄기수, 생체중, 관아수량, 괴근수량 등을 조사하였으며, 조사방법은 농촌진흥청 농업과학기술 연구조사 분석기준(RDA, 2012) 준하였다. 야콘 유전자원의 지상부 특성 결과, 초장이 92-127cm 범위를 보였으며, 줄기수는 8-12개, 경엽중은 1,433-2,855kg, 엽수는 152-195개의 범위를 보였다. 또한, 유전자원별 지하부 특성을 살펴보면, 유전자 원별 HY1이 관아수가 가장 많고, 관아수량도 352/10a으로 가장 무거웠다. 괴근수량을 살펴보면, HYL2가 총수량이 5,197 kg/10a, 상품수량이 4,213 kg/10a으로로 가장 많았으며, HYL3, HYL4, HYL5, HYL1 순이었다. 유전자원의 생리장해 결과를 조사한 결과, 열근 및 부패발생은 HYL1이 가장 심하였으며, HLY5가 상대적으로 약했다. 유전자원의 품질특성을 조사한 결과, 당도는 HYL5가 12.5로 가장 높았으며, HYL1이 8.7로 가장 낮았다. 이상의 결과를 바탕으로, 향후 야콘의 인공교배 단계에서부터 육종프로그램에 활용할 계획이다.

• Molecules and Cells
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• v.39 no.8
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• pp.581-586
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• 2016

Post-translational modifications (PTMs) of proteins are essential to increase the functional diversity of the proteome. By adding chemical groups to proteins, or degrading entire proteins by phosphorylation, glycosylation, ubiquitination, neddylation, acetylation, lipidation, and proteolysis, the complexity of the proteome increases, and this then influences most biological processes. Although small RNAs are crucial regulatory elements for gene expression in most eukaryotes, PTMs of small RNA microprocessor and RNA silencing components have not been extensively investigated in plants. To date, several studies have shown that the proteolytic regulation of AGOs is important for host-pathogen interactions. DRB4 is regulated by the ubiquitin-proteasome system, and the degradation of HYL1 is modulated by a de-etiolation repressor, COP1, and an unknown cytoplasmic protease. Here, we discuss current findings on the PTMs of microprocessor and RNA silencing components in plants.

• Pediatric Infection and Vaccine
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• v.22 no.3
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• pp.194-200
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• 2015

Purpose: Urinary tract infection (UTI) is a common bacterial infection in children and Escherichia coli is a predominant pathogen. The purpose of this study is to evaluate phylogenetic groups and virulence factors of E. coli causing UTI in children in Korea. Methods: From October 2010 to April 2013, urinary E. coli strains were isolated from the 33 pediatric patients of UTI. Multiplex polymerase chain reactions were performed to evaluate the phylogenetic groups and 5 virulence factor genes (fimH, sfa, papA, hylA, and cnf1) of E. coli. Distribution of molecular characteristics of E. coli was analyzed by clinical diagnosis and accompanying vesicoureteral reflux (VUR). Results: Most (84.8%) uropathogenic E. coli were belonged to phylogenetics group B2 and the others (15.2%) were belonged to group D. The virulence factors were distributed as: fimH (100%), sfa (100%), hylA (63.6%), cnfI (63.6%), and papA (36.4%). According to clinical diagnosis, phylogenetic distribution of E. coli strain was 92.3% of B2 and 7.7% of D in acute pyelonephritis and 57.1% of B2 and 42.9% of D in cystitis. Distribution of virulence factors was similar in both groups. In patients with acute pyelonephritis, phylogenetic distribution was similar in VUR and non-VUR group, but proportion of papA genes were lower in VUR group than that of non-VUR group (43.8% vs. 20.0%, P=0.399). Conclusions: This study provides current epidemiologic molecular data of E. coli causing pediatric UTI in Korea and will be a fundamental for understanding the pathogenesis of pediatric UTI.

• Journal of Photoscience
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• v.9 no.2
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• pp.90-93
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• 2002

Phytochrome A (phyA) and phytochrome B (phyB) perceive light and adapt to fluctuating circumstances by different manners in terms of effective wavelengths, required fluence and photoreversibility. Action spectra for induction of seed germination and inhibition of hypocotyl elongation using phytochrome mutants of Arabidopsis showed major difference. PhyA is the principal photoreceptor for the very low fluence responses and the far-red light-induced high irradiance responses, while phyB controls low fluence response in a red/far-red reversible mode. The structural requirement of their bilin chromophores for photosensory specificity of phyA and phyB was investigated by reconstituting holophytochromes through feeding various synthetic bilins to the following chromophore-deficient mutants: hy1, hyl/phyA and hyl/phyB mutants of Arabidopsis. We found that the vinyl side-chain of the D-ring in phytochromobilin interacts with phyA apoprotein. This interaction plays a direct role in mediating the specific photosensory function of phyA. The ethyl side-chain of the D-ring in phycocyanobilin fails to interact with phyA apoprotein, therefore, phyA specific photosensory function is not observed. In contrast, both phytochromobilin and phycocyanobilin interact with phyB apoprotein and induce phyB specific photosensory functions. Structural requirements of the apoproteins and the chromophores for the specific photoperception of phyA and phyB are discussed.

• The Korean Journal of Food And Nutrition
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• v.33 no.3
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• pp.223-236
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• 2020

In this study, a safety evaluation was conducted to confirm if the Enterococcus faecium CKDB003 strain obtained by selection from a mixed fermentation of fruit and milk is suitable for use as a probiotic. The MIC value for the 10 antibiotics specified in the EFSA guidance was below the acceptable cut-off value. The antibiotic resistance genes aac(6')-li, eatAv, and msr(C) exist by whole genome sequencing, but are in the chromosome and not in the plasmid, thus confirming that there is no possibility of transmission to other microorganisms. It was confirmed that cytolysin (cylA, cylB, cylI, cylL-l, cylL-s, cylM, cylR1, cylR2), aggregation substance (asa1, asp1), collagen adhesion (ace), enterococcal surface protein (esp), endocarditis antigen (efaA), hyaluronidase (hyl) and gelatinase (gelE) were not present in the genome by examining the genes of factors related to virulence. Also, the biochemical analysis showed no toxic enzyme activities, and no virulence genes were detected by the PCR method. Thus, the E. faecium CKDB003 strain can be safely used as a health functional food probiotic, based on the results of the safety assessment.

• Culinary science and hospitality research
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• v.22 no.6
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• pp.71-77
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• 2016

To determine the nutritional value of domestic tomatoes, the levels of amino acids, amino acid metabolites, and the bioactive compound ${\gamma}-aminobutyric-acid$ (GABA) were analyzed in three domestic tomato varieties (Rafito, Momotaro, and Medison). Eighteen free amino acids were found, and total free amino acid content was 3,810.21~4,594.56 mg/100 g (dry weight). L-glutamic acid (L-Glu) was the most abundant amino acid, ranging from 1,866.60 mg/100 g for Momotaro to 2,417.45 mg/100 g for Medison. The next most abundant amino acids were L-glutamine (L-Gln) and L-aspartic acid (L-Asp). The three tomato varieties had a good balance of all the essential amino acids except tryptophan. Total essential amino acid content was 274.26~472.71 mg/100 g (dry weight). The following amino acid metabolites were found: L-carnitine (L-Car), hydroxylysine (Hyl), o-phosphoethanolamine (o-Pea), phosphoserine (p-Ser), ${\beta}-alanine$ (${\beta}-Ala$), N-methyl-histidine (Me-His), ethanolamine (EtNH2),and L-citrulline(L-Cit). Large quantities of GABA were found in all three varieties: 666.95-868.48 mg/100g (dry weight). These results support the use of these tomato varieties as nutritious food materials.

• Korean Journal of Plant Resources
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• v.26 no.1
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• pp.9-18
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• 2013

The aim of this study is to provide the basic data for yacon [Samallanthus sonchifolius (Poepp. & Endl.) H. Robinson] in dietary food. The nutritional compositons, such as protein, ash, carbohydrate, dietary fiber, vitamin and fructooligosaccharide, were analyzed for 4 yacon germplasm lines. Yacon has low calories with only 46~56 kcal/100 g. The contents of water, fat, ash, protein, carbohydrate and dietary fiber were ranged 85.9~86.8%, 0.1~0.2%, 0.2~0.3%, 0.5~0.7%, 12.2~13.1% and 1.05~1.14%, respectively. The iodine-starch test did not show any color or precipitation reaction, which indicates that yacon has no starch content. However, in the absence of starch, yacon is rich in fluctooligosaccharide, which is between 9.6~11.1%. Maltose is present in the larger amount, followed by sucrose, glucose, and fructose in terms of free sugars. The analysis of minerals revealed that yacon contains potassium in the larger amount of 141~176 mg/100 g F.W., followed by magnesium at 8.2~10.6 mg, calcium, and sodium representing the least present mineral. Yacon proved to have a total of 17 types of amino acids, which are between 404.0~581.8 mg per 100 g of yacon. Glutamic acid, the main sweetening component, is present in the large amount of 94.0~182.2 mg/100 g F.W., followed by aspartic acid, arginine, and alanine. The proportion of the essential amino acid was 24.8~33.6%. Results of analysis also showed that yacon contains 0.001~0.024 mg, 0.03~0.11 mg, 0.02~0.3 mg, 0.3~0.4 mg and 14.1~20.6 mg of ${\beta}$-carotene, thiamin, riboflavin, niacin, and ascorbic acid, respectively. It is also likely to be highly used as functional food material in the future because it is abundant in both fluctooligosaccharide and antioxidants which are important functional components.

• Culinary science and hospitality research
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• v.23 no.7
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• pp.42-49
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• 2017

This study was conducted to determine the nutritional value of domestic cherry tomato varieties (Summerking, Qutiquti, and Minichal). The levels of amino acids, amino acid derivatives, and ${\gamma}-aminobutyric-acid$ (GABA) were analyzed using ion chromatography. In domestic cherry tomatoes, eighteen free amino acids were found including L-glutamic acid (L-Glu), L-glutamine (L-Gln), and L-aspartic acid (L-Asp). L-Glu was the most abundant amino acid, ranging from 1,533.17 mg/100 g to 1,920.65 mg/100 g (dry weight). The next abundant amino acids were L-Gln, ranging from 784.68 mg/100 g to 1,164.36 mg/100 g and L-Asp, ranging from 320.73 mg/100 g to 387.22 mg/100 g. Domestic cherry tomatoes contained eight essential amino acids except tryptophan and the total essential amino acid content was 297.30~432.43 mg/100 g (dry weight), which was 8.92~10.61% of total free amino acid. Several amino acid derivatives were found: L-carnitine (L-Car), hydroxylysine (Hyl), o-phosphoethanolamine (o-Pea), phosphoserine (p-Ser), ${\beta}-alanine$ (${\beta}-Ala$), N-methyl-histidine (Me-His), ethanolamine ($EtNH_2$), and L-citrulline (L-Cit). L-Car, transporting long-chain fatty acid into mitocondrial matrix, was the most abundant amino acid derivative in all domestic cherry tomatoes. A high level of GABA (313.18~638.57 mg/100 g), known as a neurotransmitter, was also found in all three domestic cherry tomatoes. These results revealed that domestic cherry tomatoes have a good balance of nutrient and bioactive compounds. Therefore, cherry tomatoes can be used as a functional food material.

• Journal of Applied Biological Chemistry
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• v.65 no.3
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• pp.121-128
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• 2022

This study explored plant-derived natural antioxidants by evaluating the antioxidant activity of Lamiaceae plant seed extracts. Plants with the percentage of filled seeds at or above 90% and seed germination at or above 50% were selected. Of the ten species studied, the total phenolic content of the seeds was high in the species Phlomis umbrosa Turcz. (6.2 mg GAEs/g of seeds) and Elsholtzia ciliata (Thunb.) Hyl. (4.5 mg GAEs/g of seeds). The total flavonoid content of the seeds was high in E. ciliata (1.0 mg QEs/g of seeds) and P. umbrosa (0.6 mg QEs/g of seeds). Based on the EC₅₀ value of the seed extracts, 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity was high in the seeds of the plants E. ciliata (27.5 ㎍/mL), Mosla dianthera (Buch.-Ham. ex Roxb.) Maxim. (29.2 ㎍/mL), and Prunella vulgaris var. lilacina Nakai (33.3 ㎍/mL). In addition, 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity was high in P. vulgaris var. lilacina (25.6 ㎍/mL), E. ciliata (25.9 ㎍/mL), and M. dianthera (27.6 ㎍/mL) seeds. The ferric reducing antioxidant power of the seed extracts was high in P. vulgaris var. lilacina (2910.4 µM Fe(II)/g of extract), E. ciliata (2836.2 µM Fe(II)/g of extract), and M. dianthera (2754.4 µM Fe(II)/g of extract). According to the cluster analysis based on antioxidant activity, the seeds of the ten species were classified into three groups, from group 1 with low antioxidant activity to group 3 with high antioxidant activity; E. ciliata, M. dianthera, and P. vulgaris var. lilacina were classified as group 3.

• Journal of Korean Philosophical Society
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• v.145
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• pp.195-215
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• 2018

Plato said that our cosmos was created from the union of nous and $anank{\bar{e}}$ in Timaeus. In addition to this, 'the third kind', namely $ch{\bar{o}}ra$, exists already with genesis before the birth of the cosmos. Plato explains this $ch{\bar{o}}ra$ with several metaphors. That is, 'receptacle', 'mother', 'gold', 'space', 'place' etc. From Aristotle to contemporary scholars, generally three types of interpretations are presented. First, $ch{\bar{o}}ra$ is a kind of a 'void.' Secondly, $ch{\bar{o}}ra$ is Aristotle's $prot{\bar{e}}$ $hyl{\bar{e}}$. Thirdly, $ch{\bar{o}}ra$ has two aspects of space and matter. I will accept the third opinion. Plato's $ch{\bar{o}}ra$ has both a spatial aspect and material aspect. $Ch{\bar{o}}ra$ is a place that accommodates all sensible things in itself. On the other hand, $ch{\bar{o}}ra$ seems to be a mother who nourishes sensible things like a child. In this, Plato lacks a logical consistency. The research of $ch{\bar{o}}ra$ can not deviate from the limits of $eik{\bar{o}}s$ logos.