• Journal of Life Science
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• v.11 no.1
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• pp.76-82
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• 2001

Fifteen species of microorganisms were isolate form the intestines of fishes, fish feed, and ferment. Eleven microorganisms except HY4, HY8, HY12, and HY13 were Gram-positive, and HY1, HY2, HY3, HY5, HY6, and HY7 produced lactic acid. The species of HY1, HY2, HY3, HY4, HY5, HY6, HY13, and HY14 showed some growth in the medium containing 1% of NaCl. Except HY6, HY7, HY8, HY12 and HY5, 10 isolates had proteolytic activity, whereas only HY13 and HY14 had lipase activity. From all the results four isolates (HY3, HY4, HY13 and HY14) were chosen for the degradation of fish wastes. There was no mutual inhibition among the microorganisms, and the optimum temperature and pH for the growth of the mixed culture were found to be 3 2$\^{C}$ and 7, respectively. Under the optimum growth conditions the maximum optical density and the maximum specific growth rate were estimated to be 2.35 and {TEX}$0.46h^{-1}${/TEX}, respectively. Major microorganisms in the mixed culture at the log-phase were HY3 and HY4, which occupied 70%. The degrading efficiency of fish waste by the mixed microorganisms was 2.3 times higher, compared to control. The total amount of free amino acids in the degraded products from fish wastes was 39g/100g protein and little odor was produced by the mixed microorganisms after 48 hours.

• Microbiology and Biotechnology Letters
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• v.35 no.2
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• pp.104-111
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• 2007

In this study, aniline dioxygenase genes responsible for initial catabolism of aniline in Burkholderia sp. HY1 and Delftia sp. HY99 were cloned and the amino acid sequences were comparatively analyzed, which already have been reported as bacteria utilizing aniline as a sole source of carbon and nitrogen, B. sp. HY1 was found to have at least a plasmid, and the plasmld-cured strain, B. sp. HY1-PC obtained using mitomycin C was tested with wild type strain to investigate whether the former maintained the degradability for aniline. This proved that the aniline oxygenase gene from B. sp. HY1 was located in chromosomal DNA, not in plasmid DNA. Aniline dioxygenase small subunits from B. sp. HY1 and D. sp. HY99 were found, based on 146 amino acids, to share 79% similarity. Notably, ado2 genes from B. sp. HY1 and D. sp. HY99 which were found to be terminal dioxygenase of aniline dioxygenase small subunit showed 99% similarity in the deduced amino acid sequences with tdnA2 of Frateuria sp. ANA-18 and danA2 of D. sp. AN3, respectively. Besides, enzyme assay and amino acid sequence analysis of catechol dioxygenase supported the previous report that B. sp. HY1 might occupy ortho-cleavage pathway using catechol 1,2-dioxygenase, while D. sp. HY99 might occupy catechol 2,3-dioxygenase for meta-cleavage pathway.

• Journal of the Korean Association of Geographic Information Studies
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• v.9 no.3
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• pp.136-145
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• 2006

SWAT includes a lot of parameters related with geography, hydrological time series, land management and water pollution, etc. So, it needs many spatial, non-spatial and time series data to run SWAT. If SWAT is operated in conjunction with GIS, we can use database which includes model input data and do all the processes which covers data creation, model input and analysis of simulation results in a system. The objective of this study is to develop HyGIS-SWAT which is the interface system to couple the SWAT model and HyGIS. To achieve this object, system operation process based on HyGIS-SWAT data model is evaluated and databases are designed and established. As a result, HyGIS-SWAT prototype system is developed. HyGIS data model and HyGIS-Model operation process can be applied effectively to the development of HyGIS-SWAT. The technologies from this study can be used as base technology to develop another HyGIS application which connect HyGIS with models.

• Microbiology and Biotechnology Letters
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• v.27 no.4
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• pp.267-272
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• 1999

The effects of Bifidobacterium longum HY8001 supplement intake on the fecal microflora and fecal bacterial enzyme activity were studied in ten healthy human volunteers, before, during and after intake (respectively for 3 weeks). During intake of B. longum HY8001 supplement, fecal, $\beta$-glucuronidase and nitroreductase activities significantly decreased 44.6%(p<0.005) and 32.3%(p<0.01), respectively. Although numbers of major bacterial groups of fecal microflora were not affected by B. longum HY8001 intake for 3 weeks, the number of Bifidobacterium was significantly increased (p<0.05). This result indicates that intake of B. longum HY8001 might be potentially beneficial for the prevention and inhibition of colon cancer and improvement of human intestinal microflora composition.

• Horticultural Science & Technology
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• v.19 no.1
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• pp.22-28
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• 2001

An optimum seeding date for carrots growing in Jeju was determined. In early growth stage, the number of leaves was more in Hyang-yang #2 cultivar (HY2) than that in the others, meanwhile that of Hukjun 5 chon (H5) and Shinhukjun 5 chon (S5) was higher in the latter half of the growing season. The weight of leaves was greater in HY2 until 2 months after seeding, but thereafter drastically increased and resulted significant difference at harvest in S5. The length of roots was greater in H5 and S5 than in HY2 when seeded on July 17 and measured after September 13, but it was similar in all cultivars when seeded on August 1 and later. The diameter of the roots was greatest in HY2, regardless of seeding dates, meanwhile no difference was found among other cultivars. The weight of roots was greatest in HY2 when seeded on July 17, August 1 or later, meanwhile it was greater in H5 after November, when seeded on August 15. The percentage of roots cracked was 20, 15, and 10% respectively for HY2, S5 and H5. The percentage of roots branched was 0.3% in HY2. The percentage of roots cracked or branched was much higher when seeded on August 1 than on July 17, and was high (40%) in HY2 and S5, compared to H5 when seeded on August 15. The content of sucrose, glucose and fructose increased until 110 days after seeding in all cultivars, but fructose and glucose contents decreased in about 135 days after seeding, but sucrose contents increased continuously even after 135 days. Sucrose content increased and fructose and glucose contents decreased in S5, but this trend was reversed after late November. Sugar contents was different among the cultivars seeded on August 15, while sucrose content decreased and glucose and fructose contents increased with time. The content of ${\beta}$-carotene was more than 12,000 IU in all cultivars, but decreased in 170 days after seeded on July 17. The content was highest in HY2 and followed by S5 and H5 in order. It was not different among the cultivars in 170 days after seeding on August 1, but was higher in S5 than the other cultivars in 170 days after seeded on late and decreased with seeding date.

• Journal of Microbiology and Biotechnology
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• v.16 no.8
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• pp.1262-1268
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• 2006

In the search for a novel cytotoxic substance from medicinal plants, HY53 ($C_{17}H_{32}O_2N_2$; molecular weight 296) was isolated from the leaves of Pata de Vaca (Bauhinia forficata). The growth of the HepG2 cells was inhibited in a dose-dependent manner when treated with 0.07 to 0.40 mM HY53 for 24 h (IC$_{50}$: 0.13 mM). Furthermore, nuclear DAPI staining revealed the typical nuclear features of apoptosis in the HepG2 cells exposed to 0.27 mM HY53, whereas a flow cytometric analysis of the HepG2 cells using propidium iodide showed that the apoptotic cell population increased gradually from 8% at 0 mM to 23% at 0.14 mM and 45% at 0.40 mM after being exposed to each concentration of HY53 for 24 h. Moreover, a TUNEL assay also exhibited the apoptotic induction of the HepG2 cells treated with HY53. To obtain further information on the HY53-induced apoptosis, the expression level of certain apoptosis-associated proteins was examined using a Western blot analysis. Treatment of the HepG2 cells with HY53 resulted in the activation of caspase-3, and subsequent proteolytic cleavage of poly(ADP-ribose) polymerase (PARP). Consequently, the results confirmed that the apoptosis in the HepG2 cells was induced by HY53 and the involvement of caspase-3-mediated PARP cleavage in the apoptotic process.

• Journal of Life Science
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• v.13 no.6
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• pp.879-888
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• 2003

In order to measure the enzyme activity of 5-epi-aristolochene hydroxylase, one of cytochrome P450 (P450) enzymes in eicitor-treated pepper cell, we used in vivo assay method and demonstrated a dramatic suppression of the activity by P450-inhibitors, ancymidol and ketocornazole. Using RT-PCR method with degenerate primer of the well conserved domains found within most P450-enzymes, and using cDNA library screening method, one distinct cDNA, being designated P450Hy01, was successfully isolated from elicitor-treated pepper cells. P450Hy01 mRNA was all induced in elicitor-treated cells whereas never induced in control cells. Moreover, levels of P450Hy01 expression were highly correlated with the levels of extracellular capsidiol production by different elicitors in cell cultures. P450Hy01 transcript was also induced by several other elicitors such as, cellulase, arachidonic acid, jasmonic acid, yeast extract as well as UV stress. P450Hy01 sequence contained high probability amino acid matches to known Plant P450 genes and ORF with a conserved FxxGxRxCxG heme-binding domain. P450Hy01 cDNA showed 98% of homology in sequence of nucleotide as well as amino acid to 5-epi-aristolochene-1, 3-hydroxylase (5EAl, 3H) which has been isolated in tobacco cells, suggesting that P450Hy01 is prominent candidate gene for P450-enzyme encoding 5EAl, 3H in pepper cell.

• Journal of Welding and Joining
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• v.14 no.5
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• pp.134-144
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• 1996

A study was performed to investigate the properties of base metal and weldment for two HSLA steels and one HY-100 steel. Tensile, yield strength and elongation of HSLA-A steel were superior to those of HY-100 steel and yield ratios in HSLA-A and HSLA-B steels were higher than HY-100 steel owing to the precipitation of $\varepsilon$-Cu phase. The impact energy of HSLA-A steel was greater at all aging temperatures than that of HY-100 steel. HSLA-A and HY-100 steels had low impact transition temperature of about -l$25^{\circ}C$ and high upper shelf energy, The peak hardness of weldment in HSLA-A, HSLA-B and HY-100 steels were Hv 299, Hv 275 and Hv 441, respectively. The hardenability of HY-100 steel was largest due to the higher amount of carbon. The y-groove test showed that HSLA steels had superior resistance to cold cracking. Toughness of weld joint at the F. L. and F. L. ＋1mm in HSLA-A was almost the same as HY-100, but those at F. L.＋3mm and F. L.＋5mm was greater in HSLA-A steel.

• Journal of Sasang Constitutional Medicine
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• v.20 no.2
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• pp.72-84
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• 2008

1. Objectives The purpose of this study was to observe the anti-oxidant effects of Hyangsayangyi-tang(HY) in SD rats. 2. Methods This experiment was used the tissue of stomach, spleen and pancreas cells of 6, 52 and 68 weeks old SD rats. Each age group was again divided into three groups. One group, as normal group, was not-treated cells, another group, as control group, was saline-treated cells, and the last group, as experimental group, was HY-treated cells. After culture for 48 hours, each groups measured the level of SOD, GSH, MDA and NO in the tissue of stomach, spleen and pancreas cells. 3. Results and Conclusions The activity of SOD were significantly increased in spleen cell of 52, 68 w-HY group, pancreas cell of 68 w-HY group and in stomach cell of 52, 68 w-HY group compared with those of the normal and the control groups. The level of GSH were significantly increased in spleen cell of 52, 68w-HY group and in pancreas cell of 68w-HY group compared with those of the normal and the control groups. The level of MDA were significantly decreased in pancreas cell of 68 w-HY group compared with those of the normal and the control groups. The level of NO were significantly decreased in spleen cell of 68 w-HY group, pancreas cell of 52, 68w-HGD groups compared with those of the control groups. According to results, HY showed anti-oxidant effect. Investigation into the clinical use of the HY is suggested for future research.

• Journal of Microbiology and Biotechnology
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• v.23 no.3
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• pp.414-421
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• 2013

Lactic acid bacteria (LAB) in fermented foods have attracted considerable attention recently as treatment options for immune diseases, the incidence of which has been increasing worldwide. The ability of 500 strains of LAB, isolated from kimchi, to induce TNF-${\alpha}$ production in peritoneal macrophages was investigated. Lactobacillus plantarum HY7712 most strongly induced TNF-${\alpha}$ production as well as NF-${\kappa}B$ activation. However, HY7712 inhibited NF-${\kappa}B$ activation in LPS-stimulated peritoneal macrophages. When HY7712 was orally treated in cyclophosphamide (CP)-immunosuppressed mice for 5 or 15 days, it reversed the body and spleen weights, blood RBC and WBC levels, and splenocyte and bone marrow cells that were reduced by CP. Orally administered HY7712 increased concanavalin A-induced T cell proliferation to 84.5% of the normal group on day 15, although treatment with CP alone markedly reduced it to 53.7% of the normal group. Furthermore, orally administered HY7712 significantly induced the expressions of IL-2 and IFN-${\gamma}$ in ConA-induced splenic cytotoxic T cells of CP-treated mice. Orally administered HY7712 restored the CP-impaired phagocytosis of macrophages in mice. Orally administered HY7712 also restored the cytotoxicity of NK and cytotoxic T cells derived from spleen and bone marrow against YAC-1 in CP-immunosuppressed mice. Based on these findings, orally administered HY7712 may accelerate the recovery of cyclophosphamide-caused immunosuppression, without evident side effects, by immunopotentiating NK and Tc cells, and may provide a mechanistic basis for using HY7712 as an alternative means in lessening chemotherapyinduced immunosuppression in cancer patients.