• 한국식품영양학회지
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• 제33권2호
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• pp.174-182
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• 2020

Probiotics improve the immune system. However, the effects of its lactic acid bacteria on atopic dermatitis relief and inflammation improvement is not fully understood. Recently, one of the probiotics, Lactobacillus helveticus HY7801 (HY7801), was found to have an anti-inflammatory effect. In this study, we investigated the effects of HY7801 on atopic dermatitis-induced animal models. After four weeks of oral administration, the group treated with HY7801 showed amelioration of the atopic dermatitis compared to the group receiving placebo. In the HY7801 treated group, the epidermal hyper-proliferation and collagen deposition were inhibited compared to the placebo group, and the secretion amount of the inflammatory factors, such as TNF-α, IL-4 were reduced. In conclusion, these results suggest that HY7801 acts as a functional probiotic via amelioration of the atopic dermatitis such as a decrease of epidermal hyper-proliferation, and collagen deposition and anti-inflammatory effects.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2009년도 학술발표회 초록집
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• pp.1327-1331
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• 2009

SWAT(Soil and Water Assessment Tool)은 대규모의 복잡한 유역에서 장기간에 걸친 다양한 종류의 토양과 토지이용 및 토지관리 상태에 따른 물과 유사 및 농업화학물질의 거동이 토지관리방법에 미치는 영향을 예측하기 위해 미국 농무성 농업연구소(USDA Agricultural Research Service, ARS)의 Jeff Arnold에 의해 1990년대 초에 개발된 유역모델이며, 최근에는 SWAT2005 모형이 개발되었다. SWAT 모형은 SWAT2000 모형을 구동하기 위한 ArcView 3.2a 기반의 인터페이스 시스템이 널리 이용되어 왔으나, 최근에는 SWAT2005 모형과 ArcGIS를 연계 운영하기 위한 ArcSWAT(Winchell et al, 2008)이 개발된 바 있다. 국내에서는 GIS와 데이터베이스를 기반으로 SWAT2000 모형의 구동 및 입출력 자료의 관리 등을 효과적으로 수행할 수 있는 SWAT2000 모형과 HyGIS(Hydro Geographic Information System)를 연계시스템인 HyGIS-SWAT(김경탁과 최윤석, 2006)이 개발된 바 있다. 본 연구에서는 최근 들어 SWAT2005 모형의 활용성이 높아짐에 따라 HyGIS와 SWAT2005 모형의 연계시스템을 개발하고자 하며, 이를 통해서 SWAT2005 모형 구동의 효율성과 편의성을 증대시킬 뿐만 아니라, 개선된 SWAT2005 모형으로 기존의 HyGIS-SWAT을 개선하고자 한다.

• 한국재료학회:학술대회논문집
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• 한국재료학회 2011년도 추계학술발표대회
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• pp.35.2-35.2
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• 2011

In this study, hyaluronic acid (HyA) - Gelatin (Gel) hydrogels were prepared at ratio of 15:85 with the goal of obtaining a high uniform porosity and porous biocompatibility scaffold for bone tissue engineering applications. In order to develop a proper scaffold for bone implant application, a HyA-Gel hydrogel loaded in sponge Biphasic Calcium Phosphate (BCP) was prepared. To assay the cytocompatibility and cell behavior on the HyA-Gel hydrogel and HyA-Gel/BCP scaffold, cell attachment and spreading of MSCs seeded on the scaffolds were studied. An invivo study was performed for HyA-Gel/BCP scaffolds after 1 and 3 months implantation. Our results provide a novel and simple method to obtain an adequate scaffold for osteoblast cells and indicate that HyA-Gel hydrogel and HyA-Gel/BCP scaffold could be a good candidate for bone tissue engineering scaffolds.

• 환경영향평가
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• 제19권3호
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• pp.223-230
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• 2010

Trajectory dispersion models are used for the dispersion calculations in air quality assessments, Yellow-sand modeling, environmental planning and the emergency response. Meso-scale forcing and coastal circulations are calculated by trajectory model in the East Asia region. In this study the meteorological fields (GDAS and MM5) coupled to the trajectory model (HY-SPLIT) are applied to simulate the transport and the dispersion. Seoul is selected as a starting point of the HY-SPLIT. The sensitivity studies are performed by conducting an ensemble of simulations using the GDAS and the MM5 model for the same dispersion cases. The results in this study show a significant difference depending on the resolution of meteorological models. Additionally, in most cases of the compared tionally,results from MM5 and GDAS, the absolute and relative distance, shows significant difference and the difference increased with the increasing distance of HY-SPLIT. Therefore, for the case of small domai for twi d field distefbution over complex terrai, should be used only high model temporal or spatial resolution to improve the HY-SPLIT model results.

• Journal of Microbiology and Biotechnology
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• 제14권5호
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• pp.978-984
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• 2004

In the course of screening for a novel inhibitor of CDC2, HY558-1 was isolated from a culture broth of Penicillium minioluteum F558. Moreover, it was found that HY558-1 had an effect on both the cell cycle regulation and apoptosis of human cervical adenocarcinoma HeLa cells. A flow cytometric analysis of HeLa cells revealed appreciable cell cycle arrest at the G1 and G2/M phases following treatment with HY558-1. Furthermore, DNA fragmentation due to apoptosis was observed in HeLa cells treated with HY558-1. To obtain further information on the cell cycle arrest and apoptotic induction induced by HY558-1, the expression of certain cell cycle and apoptosis-associated proteins was examined using a Western blot analysis. The results revealed that HY558-1 inhibited the phosphorylation of pRb and decreased the expression levels of CDK2, CDC2, and cyclin A in the cell cycle progression. It was also shown that the level of $p21^{WAF1/CIP1}$ was increased in HeLa cells treated with 0.52 mM of HY558-1. Accordingly, HY558-1 was found to inhibit the proliferation of HeLa cells through the induction of G1 phase arrest by inhibiting pRb phosphorylation via an upregulation of $p21^{WAF1/CIP1}$, and G2/M phase arrest by directly inhibiting CDC2 and cyclin A. Moreover, HeLa cells treated with 0.52 mM of HY558-1 exhibited apoptotic induction associated with the cleavage of Bid and release of cytochrome c from mitochondria into the cytosol. Subsequent investigation of the activation of caspase-3 and cleavage of poly (ADP-ribose) polymerase (PARP) suggested that the mitochondrial pathway was primarily involved in the HY558-1-induced apoptosis in HeLa cells.

• 생명과학회지
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• 제32권4호
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• pp.285-289
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• 2022

본 연구에서는 인천시 영흥도에서 채취한 해수시료에서 분리한 한천분해세균의 생육특성과 균주가 생산하는 agarase 특성을 분석하였다. 분리된 균주의 16S rRNA 유전자 염기서열은 Agarivorans 속 세균과 95% 유사하여, 분리된 균주를 Agarivorans sp. HY-1으로 명명하였다. Agarivorans sp. HY-1을 Marine broth 2216 배지에서 27℃, 250 rpm 조건으로 배양하니 생장은 1일차에, 효소활성은 2일차에 최대를 보였다. 조효소액은 균주 배양액에서 세포외로 분비되는 agarase로 준비하였다. Agarivorans sp. HY-1균주의 세포외 agarase는 40℃와 pH 7.0 (20 mM Tris- HCl)에서 최대의 활성을 보였다. 한천분해효소는 20, 30, 40, 50, 60 및 70℃에서 각 64, 91, 100, 97, 89 및 60%의 상대활성을 나타냈으며, pH 5, 6, 7 및 8에서는 79, 95, 100 및 55%의 활성을 나타냈다. 이 agarase는 20, 30, 40℃에서 2시간 열처리를 하였을 때 86% 이상의 잔존활성을 보였으며 50℃에서는 2시간 후 44% 이상의 잔존활성을 보였다. TLC 분석 결과, Agarivorans sp. HY-1는 α-agarase를 생산하는 것이 확인되었다. α-Agarase의 분해산물은 항암 활성 및 항산화 효과를 지니고 있으므로, Agarivorans sp. HY-1 균주와 agarase는 유용하게 이용할 수 있을 것으로 기대된다.

• Corrosion Science and Technology
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• 제10권5호
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• pp.157-161
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• 2011

HY-Galume is a brand name of 55%Al-Zn coated steel sheets produced by Hyundai HYSCO. Heavy gauged HY-Galume having thickness around 2 millimeters is used as a steel casing for the electrical distribution panel in the construction market. It requires a strict surface quality because it is used indoors. In this paper, several troubleshooting experiences in producing heavy gauged HY-Galume are proposed.

• Journal of Ginseng Research
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• 제47권5호
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• pp.683-683
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• 2023

• 원예과학기술지
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• 제19권1호
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• pp.22-28
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• 2001

제주에서 당근의 적정 파종기를 파악하기 위한 실험을 하였다. 엽수는 생육초기에는 '향양 2호(HY2)'가 다소 많았으나 후반기로 갈수록 오히려 '흑전 5촌(H5)'과 '신흑전5촌(S5)'에서 많아지는 편이었다. 엽중은 파종후 2개월까지는 'HY2'가 무거웠지만 후반기로 갈수록 'S5'의 증가율이 현저히 커서 후기에는 유의차를 보였다. 근장은 1차파종에서는 9월 13일부터 'H5'과 'S5'가 'HY2'에 비하여 길었으며 2,3차 파종기에서는 3품종이 거의 비슷한 경향을 보였다. 근경은 파종기 모두에서 품종간에는 큰 차이가 없었다. 근중도 1차, 2차파종기때는 역시 'HY2'가 무거웠고 3차파종기에서도 11월 이후부터는 'H5'이 더 무거운 편이었다. 1차파종인 경우 'HY2'가 20% 'S5'는 15%, 'H5'는 10%의 열근율을 나타내었으나 기근율은 'HY2'가 0.3%에 불과 하였다. 2차파종인 경우는 1차파종때보다 열근율과 기근율이 훨씬 높았고 특히 3차 파종인 경우는 'HY2', 'S5'는 40%의 높은 발생율을 보였다. 당함량은 3품종 모두 파종후 110여일경 까지는 sucrose, glucose 및 fructose가 증가하다가 135일을 전후하여 fructose, glucose는 감소하였으나 sucrose는 계속 증가하는 경향을 보였다. 품종별로 보면 'S5'인 경우 sucrose가 크게 증가하고 fructose, glucose는 감소하였으나 하순부터는 sucrose가 감소하고 fructose, glucose는 서서히 증가하였다. 그러나 3차파종기부터는 3품종 모두에서 당함량에는 차이는 있었으나 오히려 sucrose가 감소하고 glucose와 frucose가 증가함을 보였다. ${\beta}$-carotene 함량은 파종후 110일경에는 1차파종인 경우 'HY2', 'S5', 'H5', 순으로 거의 12,000IU를 웃돌았고 170일경에는 감소되는 경향을 보였다. 2차파종은 170일경에는 품종간에 큰 차이가 없었고 3차 파종기에서도 170일이 지난 후 'S5'는 다소 증가하는 편이었지만 파종기가 늦을수록 ${\beta}$-carotene 함량이 적어지는 경향을 보였다.

• 사상체질의학회지
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• 제25권4호
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• pp.343-358
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• 2013

Objectives This study aimed to observe the effect of Hyangsayangwi-tang on the cisplatin-induced gastrointestinal dysfunctions in rats. Methods Four groups(each of 8 rats per group) were used in this study. Saline and distilled water treated control rats are Intact vehicle control group. Delayed gatrointestinal mortility was induced by intraperitoneal treatment of cisplatin 2mg/kg, once a week for 5 weeks(Cisplatin control group). Hyangsayangwi-tang aqueous extracts(HY) were orally administered in a volume of 5ml/kg, once a day for 14 days from 4th ciplatin treatmernt(HY group). Ondansetron 1mg/kg was subcutaneously treated, in a volume of 1ml/kg, as same as HY(ondansetron group). We measured the body weights, intestinal charcoal transit ratio, fecal parameters, fundus MDA, GSH contents and SOD, CAT activities, TPH and MAO activities, pyloric gastrin and serotonin contents with their immunoraective cells, colonic serotonin-immunoreactive cells, the histopathology of pylorus, fundus mucosa and colon. Results and Conclusions (1) The body weight gains, the small intestinal charcoal transfer rates, the fecal parameters(numbers, weights and water contents) were increased in HY, ondansetron group. (2) The inhibit of fundus antioxidant defense systems by cisplatin were decreased in HY, ondansetron group. (3) The pyloric TPH activities were increased and the pyloric MAO activities were decreased in HY group. (4) The pyloric gastric contents and the gastrin-immunoreactive cells were increased in HY group. And the pyloric serotonin contents and the pyloric and colonic serotonin-immunoreactive cells were decreased in HY group. (5) The pyloru atrophic changes and the gastric surface erosive damage regions by cisplatin were favorably inhibited by treatment of HY. HY, a representative Soeumin prescription improve GI dysfunctions and constipation retarded by cisplatin through modulations of GI enterochromaffin cells, serotonin and gastrin-producing cells and antioxidative systems. Especially HY showed the highest favorable effects more than those of ondansetron.