• Journal of Life Science
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• v.13 no.6
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• pp.879-888
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• 2003

In order to measure the enzyme activity of 5-epi-aristolochene hydroxylase, one of cytochrome P450 (P450) enzymes in eicitor-treated pepper cell, we used in vivo assay method and demonstrated a dramatic suppression of the activity by P450-inhibitors, ancymidol and ketocornazole. Using RT-PCR method with degenerate primer of the well conserved domains found within most P450-enzymes, and using cDNA library screening method, one distinct cDNA, being designated P450Hy01, was successfully isolated from elicitor-treated pepper cells. P450Hy01 mRNA was all induced in elicitor-treated cells whereas never induced in control cells. Moreover, levels of P450Hy01 expression were highly correlated with the levels of extracellular capsidiol production by different elicitors in cell cultures. P450Hy01 transcript was also induced by several other elicitors such as, cellulase, arachidonic acid, jasmonic acid, yeast extract as well as UV stress. P450Hy01 sequence contained high probability amino acid matches to known Plant P450 genes and ORF with a conserved FxxGxRxCxG heme-binding domain. P450Hy01 cDNA showed 98% of homology in sequence of nucleotide as well as amino acid to 5-epi-aristolochene-1, 3-hydroxylase (5EAl, 3H) which has been isolated in tobacco cells, suggesting that P450Hy01 is prominent candidate gene for P450-enzyme encoding 5EAl, 3H in pepper cell.

• Journal of Climate Change Research
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• v.4 no.2
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• pp.177-188
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• 2013

In this research, forest cover distribution change, forest volume and carbon stock in Yongin-city, Gyeonggi procince were estimated focused on the forest of Yongin-City using forest type map and HyTAG model in relation to climate change. Present forest volume of Yongin-city was estimated using the data from $5^{th}$ Forest Type Map and Korean National Forest Inventory (NFI). And for the future 100 years potential forest distribution by 10-year interval were estimated using HyTAG model. Forest volume was also calculated using algebraic differences form of the growth model. According to the $5^{th}$ Forest Type Map, present needleleaf forest occupied 37.8% and broadleaf forest 62.2% of forest area. And the forest cover distribution after 30 years would be changed to 0.13% of needleleaf forest and 99.97% of broadleaf forest. Finally, 60 years later, whole forest of Yongin-city would be covered by broad-leaf forest. Also the current forest carbon stocks was measured 1,773,862 tC(56.79 tC/ha) and future carbon stocks after 50 years was predicted to 4,432,351 tC(141.90 tC/ha) by HyTAG model. The carbon stocks after 100 years later was 6,884,063 tC (220.40 tC/ha). According to the HyTAG model prediction, Pinus koraiensis, Larix kaempferi, Pinus rigida, and Pinus densiflora are not suitable to the future climate of 10-year, 30-year, 30-year, and 50-year later respectively. All Quercus spp. was predicted to be suitable to the future climate.

• Korean Chemical Engineering Research
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• v.54 no.5
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• pp.712-718
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• 2016

Platinum catalysts supported on the mesoporous material synthesized from Y zeolite were applied to synthesis of jet-fuel through n-octadecane hydroupgrading. The mesoporous aluminosolicate, $MMZ_{HY}$ was synthesized using Y zeolite as its framework source. The effect of the addition of Mg to $Pt/MMZ_{HY}$ catalyst for n-octadecane hydroupgrading was investigated. Catalyst characterization was performed with X-ray diffraction, $N_2$ adsorption, temperature-programmed reduction in hydrogen flow, temperature-programmed desorption of ammonia, and infrared spectroscopy of adsorbed pyridine. The high yield of jet-fuel over the $PtMg(2.0)/MMZ_{HY}$ can be attributed not only to the higher dispersion of Pt metal and higher reducibility, but also the higher amount of acid sites and higher strength of acid sites. The selectivity to iso-paraffin in the jet-fuel fraction could be reached above 80% over the optimized $PtMg/MMZ_{HY}$ catalyst.

• Journal of Microbiology and Biotechnology
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• v.12 no.3
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• pp.470-475
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• 2002

In the course of screening for a novel cell cycle inhibitor, a potent Cdk 1 inhibitor, HY558, was found from the culture broth of Penicillium minioluteum F558 isolated from a soil sample. The molecular ion of HY558 was identified at m/z 329 (MH+) with a molecular formula of $C_20H_44ON_2$. HY558 exhibited selective antiproliferative effects on various human cancer cell lines. Its $IC_50$ values were estimated to be 0.29 mM on HepG2, 0.30 mM on HeLa, 0.30 mM on HL6O, 0.33 mM on HT-29, and 0.25 mM on AGS cells. Interestingly, Hy558 demonstrated no antiproliferative effect with normal lymphocytes used as the control, and a low level of inhibition on the proliferation of A549 cancer cells. A flow cytometric analysis of HepG2 cells revealed an appreciable arrest of cells at the G1 and G2/M phases of the cell cycle following treatment with Hy558. furthermore, DNA fragmentation due to apoptosis was observed in HeLa cells treated with 0.46 mM of HY558.

• Applied Chemistry for Engineering
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• v.29 no.2
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• pp.248-251
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• 2018

Catalytic pyrolysis of the waste paper cup containing coffee residual (WPCCCR) was performed using a fixed bed reactor and pyrolyzer-gas chromatography/mass spectrometry (Py-GC/MS). Non-catalytic pyrolysis of WPCCCR produced a large amount of oil together with gas and char. The use of both HZSM-5 and HY decreased the yields of oil and increased the yield of gas due to the additional catalytic cracking. Owing to the acidic catalytic properties of HZSM-5 and HY, catalytic Py-GC/MS analysis of WPCCCR increased the selectivity to aromatic hydrocarbons in product oil. Owing to properties of HZSM-5 having a stronger acidity and medium pore size, the catalytic pyrolysis of WPCCR over HZSM-5 produced much larger amounts of aromatic hydrocarbons than that of using HY.

• Journal of Welding and Joining
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• v.14 no.3
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• pp.48-57
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• 1996

A study was made to examine the characteristics of base metal and stress relief cracking(SRC) of heat affected zone(HAZ) for HY-100 and Cu-bearing HSLA-100 steels. The Gleeble thermal/mechanical simulator was used to simulate the SRC/HAZ. The details of mechanical properties of base plate and SRC tested specimens were studied by impact test, optical microscopy and scanning electron microscopy. The specimens were aged at $650^{\circ}C$ for HSLA-100 steel and at $660^{\circ}C$ for HY-100 steel and thermal cycled from $1350^{\circ}C$ to $25^{\circ}C$ with a cooling time of $\Delta$t_${800^{circ}C/500^{circ}C}$=21sec. corresponds to the heat input of 30kJ/cm. The thermal cycled specimens were stressed to a predetermined level of 248~600MPa and then reheated to the stress relief temperatures of $570~620^{\circ}C$. The time to failure$(t_f)$ at a given stress level was used as a measure of SRC susceptibility. The strength, elongation and impact toughness of base plate were greater in HSLA-100 steel than in HY-100 steel. The time to failure was decreased with increasing temperature and/or stress. HSLA-100 steel was more susceptible to stress relief cracking than HY-100 steel under same conditions. It is thought to be resulted from the precipitation of $\varepsilon$-Cu phase by dynamic self diffusion of solute atoms. By the precipitation of $\varepsilon$-Cu phase, the differential strengthening of grain interior relative to grain boundary may be greater in the Cu-bearing HSLA-100 steel than in HY-100 steel. Therefore, greater strain concentration at grain boundary of HSLA-100 steel results in the increased SRC susceptibility. The activation energies for SRC of HSLA-100 steel are 103.9kcal/mal for 387MPa and 87.6kcal/mol for 437MPa and that of HY-100 steel is 129.2kcal/mol for 437MPa.

• Journal of Microbiology and Biotechnology
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• v.24 no.11
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• pp.1583-1591
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• 2014

Ultraviolet (UV) irradiation alters multiple molecular pathways in the skin, thereby inducing skin damage, including photoaging. In recent years, probiotics have gained interest due to their beneficial effects on skin health, such as inhibiting atopic dermatitis and improving skin immunity or inflammation. However, little is known about the effects of probiotics on UVB-induced photoaging. In this study, we evaluated the effect of Lactobacillus plantarum HY7714 against UVB-induced photoaging in human dermal fibroblasts and hairless mice. The results showed that L. plantarum HY7714 treatment effectively rescued UVB-reduced procollagen expression through the inhibition of UVB-induced matrix metalloproteinase (MMP)-1 expression in human dermal fibroblasts. Data from a western blot showed that L. plantarum HY7714 inhibited the phosphorylation of Jun N-terminal kinase, thereby suppressing the UVB-induced phosphorylation and expression of c-Jun. Oral administration of L. plantarum HY7714 clearly inhibited the number, depth, and area of wrinkles in hairless mouse skin. Histological data showed that L. plantarum HY7714 significantly inhibited UVB-induced epidermal thickness in mice. Western blot and zymography data also revealed that L. plantarum HY7714 effectively inhibited MMP-13 expression as well as MMP-2 and -9 activities in dermal tissue. Collectively, these results provide further insight regarding the skin biological actions of L. plantarum HY7714, a potential skin anti-photoaging agent.

• Journal of Microbiology and Biotechnology
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• v.26 no.11
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• pp.1829-1835
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• 2016

Dental caries is caused by cariogenic biofilm, an oral biofilm including Streptococcus mutans. Recently, the prevention of dental caries using various probiotics has been attempted. Lactococcus lactis HY 449 is a probiotic bacterium. The aim of this study was to investigate the effect of L. lactis HY 449 on cariogenic biofilm and to analyze its inhibitory mechanisms. Cariogenic biofilm was formed in the presence or absence of L. lactis HY 449 and L. lactis ATCC 19435, and analyzed with a confocal laser microscope. The formation of cariogenic biofilm was reduced in cultures spiked with both L. lactis strains, and L. lactis HY 449 exhibited more inhibitory effects than L. lactis ATCC 19435. In order to analyze and to compare the inhibitory mechanisms, the antibacterial activity of the spent culture medium from both L. lactis strains against S. mutans was investigated, and the expression of glucosyltransferases (gtfs) of S. mutans was then analyzed by real-time RT-PCR. In addition, the sucrose fermentation ability of both L. lactis strains was examined. Both L. lactis strains showed antibacterial activity and inhibited the expression of gtfs, a nd t he d ifference b etween both strains did not show. In the case of sucrose-fermenting ability, L. lactis HY 449 fermented sucrose but L. lactis ATCC 19435 did not. L. lactis HY 449 inhibited the uptake of sucrose and the gtfs expression of S. mutans, whereby the development of cariogenic biofilm may be inhibited. In conclusion, L. lactis HY 449 may be a useful probiotic for the prevention of dental caries.

• Korean journal of applied entomology
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• v.46 no.2
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• pp.303-311
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• 2007

From the course of screening of useful xylanase producing microorganism from a phytophagous longicorn beetle, we isolated an extra-cellular xylanase producing strain, Paenibacillus sp. HY-8 from the intestine of Moechotypa diphysis adult. On the basis of morphological, biochemical and phylogenetic studies of the new isolate was identified as a Paenibacillus species. Production of xylanase in this strain was strongly induced by adding xylan to the growth medium and repressed by glucose or xylose. The highest xylanase production was attained in the M9 media containing 1% yeast extract and 0.5% birchwood xylan when cultured at $25^{\circ}C$ for 24 hrs. HY-8 producing xylanase showed superior hydrolytic activities against various plant source feedstuff than control xylanase produced by Tricoderma sp. at pH 6.0.

• Proceedings of the Korea Water Resources Association Conference
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• 2016.05a
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• pp.316-316
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• 2016

HyGIS는 DEM 기반의 수문지형처리를 중심으로 다양한 모형을 연계할 수 있도록 구성된 S/W 모음이다. 이는 한국건설기술연구원을 비롯한 다수의 기관 및 연구원들이 노력한 성과물이다. 본 연구는 기존 HyGIS 연구과정에서 도출된 성과물의 실용화 사업화를 위한 방안을 연구하였다. 이를 위하여 S/W 테스팅, 오픈소스 도입, 클라우드 컴퓨팅으로 나누어 접근하였다. 먼저 S/W의 테스팅에 있어서 기존 개발 소스코드는 블랙박스 테스트 방식의 동등 클래스 분할, 경계 값 분석 등 일부 모듈에 대한 단위 테스트와 제한적인 통합테스트가 수행된 바 있다. 보다 체계적인 테스트 단계로서 화이트박스 테스트 개념 중 문장/분기/조건 커버리지에 대하여 검토하였으며, 실제 소스코드 중 핵심 구간에 대한 적용 및 정량화를 통하여 현 수준을 객관적으로 진단하였고 보완 방안을 도출하였다. 오픈소스 적용을 위하여 QGIS, MapWindow 등 공간정보 분야의 최신 오픈소스 모듈을 비교 검토하였다. 적용 단계는 이를 기존 HyGIS S/W에 반영시키는 과정이며, S/W 관점에서는 컴포넌트 모듈의 대체라고 표현될 수 있다. 대규모의 전환 비용이 발생되므로 적용 후보에 대하여는 기능적 측면 뿐만 아니라 마이그레이션 비용과 중장기적인 유지보수 비용을 고려한 검토가 이루어 졌다. 한편 오픈소스 기술의 적용은 단순히 구성 요소 원가절감 측면만이 아닌, 중장기적 유지보수 체계 도모 및 지속가능한 생태계로의 전환에 더 큰 의의가 있다. 마지막으로 클라우드 컴퓨팅 기술의 적용 분야이다. HyGIS 입력 Data의 공급을 위한 인프라로서 자체 구축 인프라가 아닌 IaaS 클라우드인 Blob Storage 및 CDN을 시험 적용하였다. 클라우드를 활용함으로써 초기 비용을 최소화하고 합리적 비용으로 유연한 확장이 가능한(Scale Out, Scale Up) 구조를 취하게 되었다. 또한 입력 Data 공급 서버를 위한 Storage 측면만이 아니라 S/W의 배포에 있어서도 클라우드 컴퓨팅 기술을 활용하고자 시도하였다. 클라우드 기술을 활용하여 HyGIS S/W가 설치된 VM(Virtual Machine)자체를 임대하는 방식으로 시험 구성 되었다. VM에 대한 RDP 프로토콜 Access에 있어서 IP기반 접근 제어를 통하여 보안을 강화하는 방안을 실험하였으며, ISO 27001, ISO 27018 등 관련 보안 규정에 부합하는 서비스 제공이 가능하도록 검토하였다. 이러한 클라우드 VM방식 서비스를 통하여 Package형 S/W 뿐만 아니라 Subscription 방식의 서비스 제공 방식을 병행할 수 있다. 사용자에게는 S/W 설치 및 H/W Lock 구비 과정이 생략되는 이점이 있다.