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http://dx.doi.org/10.5656/KSAE.2007.46.2.303

Isolation and Characterization of Xylanase-producing Paenibacillus sp. HY-8 from Moechotypa diphysis  

Heo, Sun-Yeon (Biological Resource Center, KRIBB)
Oh, Hyun-Woo (Biological Resource Center, KRIBB)
Park, Doo-Sang (Biological Resource Center, KRIBB)
Kim, Hyang-Mi (Biological Resource Center, KRIBB)
Bae, Kyung-Sook (Biological Resource Center, KRIBB)
Park, Ho-Yong (Biological Resource Center, KRIBB)
Publication Information
Korean journal of applied entomology / v.46, no.2, 2007 , pp. 303-311 More about this Journal
Abstract
From the course of screening of useful xylanase producing microorganism from a phytophagous longicorn beetle, we isolated an extra-cellular xylanase producing strain, Paenibacillus sp. HY-8 from the intestine of Moechotypa diphysis adult. On the basis of morphological, biochemical and phylogenetic studies of the new isolate was identified as a Paenibacillus species. Production of xylanase in this strain was strongly induced by adding xylan to the growth medium and repressed by glucose or xylose. The highest xylanase production was attained in the M9 media containing 1% yeast extract and 0.5% birchwood xylan when cultured at $25^{\circ}C$ for 24 hrs. HY-8 producing xylanase showed superior hydrolytic activities against various plant source feedstuff than control xylanase produced by Tricoderma sp. at pH 6.0.
Keywords
Paenibacillus sp. HY-8; Moechotypa diphysis; Xylanase; Insect symbiotic microorganism;
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