• Fisheries and Aquatic Sciences
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• v.16 no.2
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• pp.131-135
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• 2013

The presence of ostreid herpesvirus 1 (OsHV-1) and the percentage of viral DNA detected in Pacific oyster Crassostrea gigas adults were investigated monthly between May and November 2012 at three locations along the southern coast of Korea. Among 210 oysters examined by polymerase chain reaction (PCR) analysis, OsHV-1 DNA was detected in only one oyster collected in August. The low detection rate of OsHV-1 DNA was consistent with the lack of reported OsHV-1-associated disease in C. gigas cultured in Korea. The sequence of the present PCR product amplified with the C2/C6 primer pair was identical to that of OsHV-1 ${\mu}Var$ except for one nucleotide, and the sequence amplified with Del36-37F2/Del36-37R showed a 605-bp deletion as in OsHV-1 ${\mu}Var$. Although these sequence data are insufficient to determine genotype, the results suggest that the herpesvirus detected was similar to OsHV-1 ${\mu}Var$. This is the first report on the presence of OsHV-1 in adult Pacific oysters cultured in Korea.

• Journal of Veterinary Clinics
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• v.31 no.1
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• pp.40-45
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• 2014

Abalone herpes-like virus (AbHV) is a fatal disease of abalones that impose severe economic impacts on the industry of infected regions due to high mortality. The aim of this study was to quantify the risk of introducing AbHV into Korea through the importation of live abalones for human consumption by import risk analysis (IRA). Monte Carlo simulation models were developed to provide estimates of the probability that a ton of imported abalone contains at least one AbHV-infected individual, using historical trade data and relevant literatures. A sensitivity analysis with 5,000 iterations was also conducted to determine the extent to which input parameters affect the outcome of the model. Although many uncertainties were present in the data, the results indicated that, if 5,000 tons of abalone were imported from a hypothetical exporting country with low prevalence of AbHV (model 1), there would be at least one AbHV-infected abalones in 4,816 of those tons (96.3%), while there would be at least one AbHV-infected abalones in 100% of those tons imported from country with high prevalence (model 2). Sensitivity analysis indicated that for model 1, prevalence was the strongest influence factor on the predicted number of infections. For model 2, background mortality and washing to reduce the risk of surface contamination during processing were the major contributing factors. Risk management strategies need to be enforced to reduce the risk of AbHV introduction in that at least one infected abalone would remain in a consignment from country even with a low prevalence of AbHV infection. The methodology and the results presented here will contribute to improve the development of AbHV management program, and with more accurate data this IRA model will aid science-based decision-making on mitigation strategies to reduce the risk of AbHV introduction in Korea.

• Journal of Microbiology and Biotechnology
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• v.5 no.5
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• pp.292-296
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• 1995

Production of poly($\beta$-hydroxybutyrate-co-$\beta$-hydroxyvalerate)[poly(HB-co-HV) from glucose and propionic acid was studied in a two-stage fed-batch fermentation using Alcaligenes eutrophus NCIMB 11599. When either glucose became sufficient or the feeding rate of propionic acid decreased, production of poly(HB-co-HV) increased but concomitantly resulted in a reduced fraction of HV. During the copolymer accumulation stage, the specific production rate of hydroxyvalerate (HV) increased up to 0.013 (g-HV/g-RCM/h) but it decreased as propionic acid was accumulated. Control of the propionic acid concentration in the medium, therefore, is considered to be one of the most important operating parameters for production of poly(HB-co-HV) with a higher HV fraction. A high titre of poly(HB-co-HV) (85.6 g/I) with HV fraction of 11.4 mol$%$ could be obtained in 50 h by controlling the propionic acid concentration at 1 to 4 g/I.

• Journal of Microbiology
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• v.39 no.1
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• pp.79-82
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• 2001

The influence of levulinic acid (LA) on the production of copolyester consisting of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) by Ralstonia eutropha was investigated. Addition of LA into the culture medium greatly increased the molar fraction of 3HV in the copolyester, indicating that LA can be utilized as a precursor of 3HV. In shake flask culture, the 3HV content in the copolyester increased from 7 to 75 mol% by adding 0.5 to 4.0 g/L LA to the medium containing fructose syrup as a main carbon source. A maximal copolyester concentration of 3.6 g/L (69% of dry cell weight) was achieved with a 3HV content of 40 mo1% in a jar fermentor culture containing 4.0 g/L of LA. When LA (total concentration, 4 g/L) was added repeatedly into a fermentor culture to maintain its concentration at a low level, the copolyester content and the 3HV yield from LA reached up to 85% of dry cell weight and 5.0 g/g, respectively, which were significantly higher than those when the same concentration of the LA was supplied al1 at once. The present results indicated that LA is more effective than propionate or valerate as a cosubstrate fur the production of copolyesters with varying molar fractions of 3HV by R. eutropha.

• Proceedings of the IEEK Conference
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• 2001.06a
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• pp.29-32
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• 2001

This paper has simulated the PER(Packet Error Rate), ratio of received packet and payload BER of Bluetooth system with packet types of ACL(Asynchronous Connection Less) and SCO(Synchronous Connection Oriented) link over wireless Ad-hoc environment. AWGN(Additive White Gaussian Noise) and Rayleigh fading are considered as channel model, and the analysis is based on the baseband model of Bluetooth system. In terms of PER and ratio of received packet, performance of DM1 packet is almost same as those of HV1, HV2 and HV3 packets, the performances of the other packets depend on the packet types. In terms of payload BER performance, there is no difference among HV2 packet of SCO link and DM1, DM3, DM5 packets of ACL link. Moreover, there is no difference among HV3 packet of SCO link and DM1, DM3, DM5 packets of ACL link, too.

• Journal of Crop Science and Biotechnology
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• v.10 no.1
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• pp.50-56
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• 2007

Barley S-adenosylmethionine synthetase1 gene, which was differentially expressed in seed development of extra early barley, was regulated by the phytohormones and abiotic stresses. In order to identify the regulation regions which were involved in transcriptional control of the phytohormones and abiotic stresses, we isolated 1459 bp fragment of HvSAMS1 gene promoter using genome walking strategy and deletion series were constructed. Deleted upstream fragments(-1459, -1223, -999, -766, -545, -301 bp) were fused to the GUS reporter gene and evaluated via Agrobacterium-mediated transient expression assay. Increased GUS activity of HvSMAS1 promoter -301/GUS construct under each of NaCl, $GA_3$, ABA and ethylene application was found. However, GUS activity was negligible in the leaves transformed with the HvSMAS1 promoter(-1459, -1223, -999, -766 and -545)/GUS constructs. No significant induction of GUS activity was observed for the ethionine and spermidine treatments. In order to locate promoter sequence of the HvSAMS1 gene that was critical for the activation of gene expression, deletion and addition promoter derivatives(+, includes 43 bp of 5' ORF) of the HvSAMS1 gene fused to the GUS reporter gene were applied. The tobacco leaves which harbored the additional HvSAMS1 promoter(-1459+, -1459 to -546, -545+ and -301+)/GUS construct did not significantly induce GUS activity as compared to the HvSAMS1 promoter(-1459, -545 and -301)/GUS constructs under each of NaCl, ABA and $GA_3$ treatment. However, the GUS activity was high in the tobacco leaves which harboring the -211 to -141 regions of the HvSAMS1 promoter. This result suggested that HvSAMS1 gene expression might be regulated by this region(from -211 to -141).

• Korean Journal of Environmental Agriculture
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• v.37 no.1
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• pp.9-14
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• 2018

BACKGROUND: The use of green manure and compost as organic fertilizer may increase crop yield and soil fertility due to improved soil nutrient availability and soil organic matter content (SOM). This study aimed to investigate the effects of hairy vetch (Vicia villosa L.) and compost application on red pepper growth, yield, fruit quality and soil health. METHODS AND RESULTS: The treatments were no fertilizer (CON), chemical fertilizer (CF), hairy vetch (HV), and livestock compost+HV (LC+HV). Red pepper seedlings (70 days old) were transplanted and maintained in experimental plots for 140 days. Plant dry weight, micro- and macronutrient contents of plants and soil chemical properties were determined. All fertilizer treatments significantly increased plant dry weight. Fruit yield was significantly highest with HV treatment. As for nutrient content, plants in HV and LC+HV treatments have significantly higher K and Ca contents than the other treatments. Regarding soil properties, HV and LC+HV application significantly altered the soil chemical properties. Significantly higher SOM was observed in HV and LC+HV treated soils. CONCLUSION: The results of this study suggest that short-term application of hairy vetch and compost is an effective alternative to the conventional chemical fertilizer to increase fruit yield red pepper and improve soil health.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.37 no.3
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• pp.429-436
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• 2013

This study considers how the fatigue behavior and probabilistic properties of SAE1055 steel are related to its hardness level. SAE1055 steel was heat-treated using induction hardening. Five types of specimens were prepared (A: base material, B: through hardened material with HV390, C: through hardened material with HV510, D: through hardened material with HV700, and E: surface hardened material with HV700). Fatigue tests were performed under a stress ratio of R = -1 using a 4-point rotary bending fatigue tester. The fatigue behaviors were greatly influenced by the hardness, but the fatigue limit did not increase over a hardness of HV510. In addition, the effect of the hardness level on the failure mechanism was evaluated using a scanning electron microscope. The probabilistic properties of the fatigue life were investigated using a probabilistic S-N approach, and the effect of the hardness level on these properties was evaluated using a residue analysis.

• Korean Journal of Organic Agriculture
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• v.19 no.spc
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• pp.153-156
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• 2011

Supplying rate of nitrogen at HV was 172.8 kg $ha^{-1}$, HV/B was 64.3 kg $ha^{-1}$ and B was 38.6 kg $ha^{-1}$. The Rice yield was 7.05 ton $ha^{-1}$ when the nitrogen supply was the largest with HV and 5.42 ton $ha^{-1}$ was produced on HV/B. The chemical characteristics of soil have lower pH and exchangeable cations(Ca and Mg) at B, HV and HV/B, rather than at CF because green manure was applied at the former step. However, the physical characteristics of the soil and the porosity showed different tendency which was that it was better at the green manure crops than CF. Nitrogen nutrient balance was showed the most balanced at CF and field of application of green manure crops were required the appropriate management if future crops would be cultivated because nitrogen nutrient could be exhausted or accumulated.

• Korean Journal of Agricultural Science
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• v.38 no.4
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• pp.703-709
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• 2011

This study was conducted at Korea's typical soil, loamy soil, to figure out the effect of how barley green manure (B), hairy vetch (HV) and the mixed-planting hairy vetch with barley green manure (HV/B) affecting on the yield of rice, usage of nutrient and soil characteristics. Supplying amount of nitrogen from HV, HV/B and B were 172.8 kg $ha^{-1}$, 64.3 kg $ha^{-1}$ and 38.6 kg $ha^{-1}$, respectively. Hairy vetch had the highest plant height and SPAD of rice and next was hairy vetch/barley, chemical fertilizer (CF), none fertilizer (NF), and green manure barley. The amount of rice yield was 5.51 ton $ha^{-1}$ with HV, and 4.24 ton $ha^{-1}$ with HV/B. The chemical characteristics of soil showed lower pH and exchangeable cations (Ca and Mg) at B, HV and HV/B plot rather than that of chemical fertilizer (CF) plot. However, the physical characteristics of soil and the porosity rate showed better tendency at the green manure crops than CF. Nitrogen nutrient balance was showed the most balanced at CF, otherwise field of application of green manure crops were required the appropriate nutrition management if future crops would be cultivated because nitrogen nutrient could be devastated or accumulated.