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Sex Steroids Regulate Expression of Genes Containing Long Interspersed Elements-1s in Breast Cancer Cells

  • Chaiwongwatanakul, Saichon;Yanatatsaneejit, Pattamawadee;Tongsima, Sissades;Mutirangura, Apiwat;Boonyaratanakornkit, Viroj
    • Asian Pacific Journal of Cancer Prevention
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    • v.17 no.8
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    • pp.4003-4007
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    • 2016
  • Long interspersed elements-1s (LINE-1s) are dispersed all over the human genome. There is evidence that hypomethylation of LINE-1s and levels of sex steroids regulate gene expression leading to cancer development. Here, we compared mRNA levels of genes containing an intragenic LINE-1 in breast cancer cells treated with various sex steroids from Gene Expression Omnibus (GEO), with the gene expression database using chi-square analysis (http://www.ncbi.nlm.nih.gov/geo). We evaluated whether sex steroids influence expression of genes containing an intragenic LINE-1. Three sex steroids at various concentrations, 1 and 10 nM estradiol (E2), 10 nM progesterone (PG) and 10 nM androgen (AN), were assessed. In breast cancer cells treated with 1 or 10 nM E2, a significant percentage of genes containing an intragenic LINE-1 were down-regulated. A highly significant percentage of E2-regulated genes containing an intragenic LINE-1 was down-regulated in cells treated with 1 nM E2 for 3 hours (p<3.70E-25; OR=1.91; 95% CI=2.16-1.69). Similarly, high percentages of PG or AN-regulated genes containing an intragenic LINE-1 wwere also down-regulated in cells treated with 10 nM PG or 10 nM AN for 16 hr (p=9.53E-06; OR=1.65; 95% CI=2.06-1.32 and p=3.81E-14; OR=2.01; 95% CI=2.42-1.67). Interestingly, a significant percentage of AN-regulated genes containing an intragenic LINE-1 was up-regulated in cells treated with 10 nM AN for 16 hr (p=4.03E-02; OR=1.40; 95% CI=1.95-1.01). These findings suggest that intragenic LINE-1s may play roles in sex steroid mediated gene expression in breast cancer cells, which could have significant implications for the development and progression of sex steroid-dependent cancers.

Application of IDL for Establishing the Database and Visualization System of National Wind Map (국가바람지도 데이터베이스화 및 가시화를 위한 IDL 활용)

  • Kim, Hyun-Goo;Lee, Soon-Hwan;Lee, Sang-Woo;Lee, Jong-Hyuk
    • 한국신재생에너지학회:학술대회논문집
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    • 2010.06a
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    • pp.185.2-185.2
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    • 2010
  • 한반도 국가바람지도(김현구, 2009)는 한국에너지기술연구원에서 지식경제부의 부처임무사업으로 구축되었으며 현재 웹서비스(http://www.kier-wind.org)를 통하여 정보를 제공하고 있다. 국가바람지도는 수치기상예측(NWP; Numerical Weather Prediction) 모델을 이용하여 영토, 영해에 대해 $1km{\times}1km$의 고해상도로 작성한 뒤(이순환 등, 2009) 풍력자원 정보로 재가공되었다. 한반도 국가바람지도는 5년의 장기간에 대한 시계열 수치기상예측에 의하여 구축되었기 때문에 데이터베이스(DB; database)의 효율적 관리가 필연적으로 요구된다. MM5 또는 WRF 모델의 고유 출력포맷의 자료구조는 풍력자원분석에 필요한 기상요소 외에도 대기과학자에게 필요한 수많은 기상인자를 종합적으로 포함하고 있다. 따라서 2차원 층(layer) 또는 3차원 공간분포 분석 및 계산격자인 셀(cell)에서의 1차원 시계열 분석 등 다양한 자료축출에는 비효율적인 자료구조가 된다. 이러한 자료구조의 불편을 해소하기 위해서는 기상요소별로 독립적이고 빈번한 시계열 자료 추출에 효율성을 가지며 어떤 프로그래밍 언어를 사용하든지 직관적으로 쉽게 사용할 수 있는 바람지도 데이터베이스의 재구성이 요구된다. 이에 대용량 수치자료의 처리 측면에서 장점을 가지는 과학기술 프로그래밍 언어인 IDL을 기반으로 국가바람지도의 자료구조를 효율화하여 데이터베이스화 하였으며 IDL에 내재된 그래픽 기능을 활용하여 가시화를 구현함으로써 연구개발자의 입장에서 국가바람지도의 활용성 및 효율성을 향상시키고자 하였다.

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Design and Development of Network for Housing Estate Security System

  • Nachin, Awacharin;Mitatha, Somsak;Dejhan, Kobchai;Kirdpipat, Patchanon;Miyanaga, Yoshikazu
    • 제어로봇시스템학회:학술대회논문집
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    • 2003.10a
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    • pp.1480-1484
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    • 2003
  • This paper presents the design and development of network for housing estate security system. The system can cover up to 961 houses which can be up to 1,200 meters long transfer rate of 9,600 bps. This system uses checking and warning the abnormal situation. More over this system has ability to control switch on/off the electrical equipment in the house via AC line control system. The system consists of 4 parts. The first part is a security system of each house using MCS-51 microcontroller as a central processing unit scan 32 sensors and control 8 appliances and send alarm. The MCS-51 microcontroller received control signal via telephone used DTMF circuit. The second part is distributed two levels master/slave network implementing after RS-485 serial communication standard. The protocol its base on the OSI (Open Systems Interconnection) 7 layers protocol model design focus on speed, reliability and security of data that is transferred. The network security using encrypt by DES algorithm, message sequence, time stamp checking and authentication system when user to access and when connect new device to this system. Flow control in system is Poll/Select and Stop-and-Wait method. The third part is central server that using microcomputer which its main function are storing event data into database and can check history event. The final part is internet system which users can access their own homes via the Internet. This web service is based on a combination of SOAP, HTTP and TCP/IP protocols. Messages are exchanged using XML format [6]. In order to save the number of IP address, the system uses 1 IP address for the whole village in which all homes and appliance in this village are addressed using internal identification numbers. This proposed system gives the data transfer accuracy over 99.8% and maximum polling time is 1,120 ms.

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Haenam Paddy-field KoFlux (HPK) Site with Dry Direct-Seeding: Introduction (해남 무논점파 논 KoFlux (HPK) 관측지: 소개)

  • Lee, Seung-Hoon;Kang, Minseok;Kang, Namgoo;Kim, Joon
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.20 no.1
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    • pp.18-33
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    • 2018
  • A new eddy covariance-based flux tower has been established since June 2016 in a dry direct-seeding rice paddy field in southwestern Korea - Haenam Paddy field KoFlux site (HPK). To assess the competitiveness of direct-seeding agricultural technique in the context of climate-smart agriculture, we have been monitoring the $CO_2$, $CH_4$ and energy fluxes continuously. In this communication, we (1) introduce the HPK site and (2) explain the structure and processes of the HPK-specific data processing and quality control. The latter consists of 10-steps data processing and quality control procedures following the KoFlux standardized protocol and explained each step in layman's term. The final data produced during the data processing were stored in NCAM-DAPS (National Center for AgroMeteorology-Data Acquisition and Processing System, http://daps.ncam.kr). We hope that the introduction of new HPK KoFlux site would serve as a platform to facilitate transdisciplinary efforts in the research and education associated with climate-smart agriculture toward sustainability.

WebSHArk 1.0: A Benchmark Collection for Malicious Web Shell Detection

  • Kim, Jinsuk;Yoo, Dong-Hoon;Jang, Heejin;Jeong, Kimoon
    • Journal of Information Processing Systems
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    • v.11 no.2
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    • pp.229-238
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    • 2015
  • Web shells are programs that are written for a specific purpose in Web scripting languages, such as PHP, ASP, ASP.NET, JSP, PERL-CGI, etc. Web shells provide a means to communicate with the server's operating system via the interpreter of the web scripting languages. Hence, web shells can execute OS specific commands over HTTP. Usually, web attacks by malicious users are made by uploading one of these web shells to compromise the target web servers. Though there have been several approaches to detect such malicious web shells, no standard dataset has been built to compare various web shell detection techniques. In this paper, we present a collection of web shell files, WebSHArk 1.0, as a standard dataset for current and future studies in malicious web shell detection. To provide baseline results for future studies and for the improvement of current tools, we also present some benchmark results by scanning the WebSHArk dataset directory with three web shell scanning tools that are publicly available on the Internet. The WebSHArk 1.0 dataset is only available upon request via email to one of the authors, due to security and legal issues.

HORIZON RUN 4 SIMULATION: COUPLED EVOLUTION OF GALAXIES AND LARGE-SCALE STRUCTURES OF THE UNIVERSE

  • KIM, JUHAN;PARK, CHANGBOM;L'HUILLIER, BENJAMIN;HONG, SUNGWOOK E.
    • Journal of The Korean Astronomical Society
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    • v.48 no.4
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    • pp.213-228
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    • 2015
  • The Horizon Run 4 is a cosmological N-body simulation designed for the study of coupled evolution between galaxies and large-scale structures of the Universe, and for the test of galaxy formation models. Using 63003 gravitating particles in a cubic box of Lbox = 3150 h−1Mpc, we build a dense forest of halo merger trees to trace the halo merger history with a halo mass resolution scale down to Ms = 2.7 × 1011h−1M. We build a set of particle and halo data, which can serve as testbeds for comparison of cosmological models and gravitational theories with observations. We find that the FoF halo mass function shows a substantial deviation from the universal form with tangible redshift evolution of amplitude and shape. At higher redshifts, the amplitude of the mass function is lower, and the functional form is shifted toward larger values of ln(1/σ). We also find that the baryonic acoustic oscillation feature in the two-point correlation function of mock galaxies becomes broader with a peak position moving to smaller scales and the peak amplitude decreasing for increasing directional cosine μ compared to the linear predictions. From the halo merger trees built from halo data at 75 redshifts, we measure the half-mass epoch of halos and find that less massive halos tend to reach half of their current mass at higher redshifts. Simulation outputs including snapshot data, past lightcone space data, and halo merger data are available at http://sdss.kias.re.kr/astro/Horizon-Run4.

Phylogenetic Analysis of Dendropanax morbifera Using Nuclear Ribosomal DNA Internal Transcribed Spacer (ITS) Region Sequences (Internal transcribed spacer (ITS) region의 염기서열 분석에 의한 보길도산 황칠나무의 분자 계통학적 연구)

  • Shin, Yong Kook
    • Journal of Life Science
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    • v.26 no.11
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    • pp.1341-1344
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    • 2016
  • Dendropanax morbifera is an endemic tree species of Korea, it is restricted to the southern parts of Korea. The internal transcribed spacer (ITS) region of nuclear ribosomal DNA (nrDNA) for Dendropanax morbifera grown at Bogil-do, Korea was determined. We investigated the sequence-based phylogenetic relationships of plants related and clarified its taxonomical position. The determined sequences consisted of 689 residues. ITS1 was 222 bp long while ITS2 was 233 bp long. The 5.8S rDNA was 160 bp long. The ITS region sequences of the Dendropanax species included in this study were obtained from GenBank. Oreopanax polycephalus was used as the outgroup. A pairwise alignment was calculated using the Clustal X program. A phylogenetic tree was constructed by the neighbor-joining method using the Tree view program. Sequence similarities among species including D. morbifera Bogil-do isolate showed the range 92.6 to 99.7% in sequence-based phylogenetic analysis using total 615 base pairs of ITS1, 5.8S rDNA and ITS2. D. morbifera Bogil-do isolate exhibited the highest degree of relatedness to D. chevalieri, sharing 99.7% ITS region similarity. D. morbifera Bogil-do isolate also showed to D. trifidus, sharing 99.4% ITS region similarity.

Fake News Detection for Korean News Using Text Mining and Machine Learning Techniques (텍스트 마이닝과 기계 학습을 이용한 국내 가짜뉴스 예측)

  • Yun, Tae-Uk;Ahn, Hyunchul
    • Journal of Information Technology Applications and Management
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    • v.25 no.1
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    • pp.19-32
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    • 2018
  • Fake news is defined as the news articles that are intentionally and verifiably false, and could mislead readers. Spread of fake news may provoke anxiety, chaos, fear, or irrational decisions of the public. Thus, detecting fake news and preventing its spread has become very important issue in our society. However, due to the huge amount of fake news produced every day, it is almost impossible to identify it by a human. Under this context, researchers have tried to develop automated fake news detection method using Artificial Intelligence techniques over the past years. But, unfortunately, there have been no prior studies proposed an automated fake news detection method for Korean news. In this study, we aim to detect Korean fake news using text mining and machine learning techniques. Our proposed method consists of two steps. In the first step, the news contents to be analyzed is convert to quantified values using various text mining techniques (Topic Modeling, TF-IDF, and so on). After that, in step 2, classifiers are trained using the values produced in step 1. As the classifiers, machine learning techniques such as multiple discriminant analysis, case based reasoning, artificial neural networks, and support vector machine can be applied. To validate the effectiveness of the proposed method, we collected 200 Korean news from Seoul National University's FactCheck (http://factcheck.snu.ac.kr). which provides with detailed analysis reports from about 20 media outlets and links to source documents for each case. Using this dataset, we will identify which text features are important as well as which classifiers are effective in detecting Korean fake news.

An Analysis of the Characteristics of Accredited Childcare Centers in Chungbuk Province (충청북도 어린이집의 평가인증에 영향을 미치는 특성 분석)

  • Yoo, Jaeeon
    • Journal of Families and Better Life
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    • v.32 no.5
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    • pp.15-30
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    • 2014
  • This study aims to analyze what factors are associated with child care accreditation in Chungbuk province. This study examined three questions about which factors influence the accreditation of childcare centers: 1) if they passed the accreditation criteria, 2) the total score on the accreditation criteria, and 3) the domain scores for the six domains of environment, management, process, interaction and teaching, health and nutrition, and safety. The characteristics of 1,212 childcare centers, the independent variables, were commit management, type, specialized service, maximum capacity of children, registration ratio, target age groups of children, duration, and city size. The formal expert committee evaluated objective scores for 952 childcare centers. I derived the data for this study from the i-sarang Childcare Information Portal site (http://info.childcare. go.kr/). Frequency, descriptive statistics, binary logit model, and OLS model analysis were used to examine the accreditation and scores by factors of childcare centers. The findings of the present study are as follows. First, about 80% of the 1,212 childcare centers in Chungbuk province were accredited. Second, the accreditation of the child care center was affected by the specialized service, registration ratio, and duration. Finally, the total score of accredited childcare centers was associated with the maximum capacity of children, registration ratio, and duration. The factors of childcare centers influencing sub-area scores were different by environment, interaction and teaching, health and nutrition, and safety. The present study is important because it offers preliminary data identifying accreditation characteristics that can judge problems related to the quality of service of childcare centers and suggest childcare policy implications.

Observational Performance of KMTNet - Bulge Season 2015

  • Lee, Chung-Uk;Kim, Seung-Lee;Kim, Dong-Jin;Cha, Sang-Mok;Lee, Yongseok;Lim, Jin-Sun;Lee, Dong-Joo;Park, Byeong-Gon
    • The Bulletin of The Korean Astronomical Society
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    • v.41 no.1
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    • pp.52.4-53
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    • 2016
  • 한국천문연구원에서는 직경 1.6m 광시야 망원경과 3.4억 화소 CCD 카메라로 구성된 동일한 성능의 외계행성 탐색시스템 3대를 남반구 관측소에 설치 완료하였다. 2014년 9월 칠레에 1호기 설치를 시작으로 2014년 12월과 2015년 5월에 남아공 및 호주에 2호기와 3호기를 각각 설치하였다. 외계행성 탐색시스템이 설치된 3개 관측소는 경도상 위치가 적당히 3등분 되어있어 동일한 천체를 최대 24시간 연속하여 관측 가능하다. 우리는 이 시스템이 가진 장점을 최대한 살릴 수 있는 연구주제를 선정하여 2015년 10월부터 본격적으로 관측을 수행해오고 있다. 3월부터 10월에는 3개 관측소에서 우리은하 중심부를 24시간 연속 관측하여 미시중력렌즈 방법을 이용한 외계행성 탐색연구를 수행하고 은하 중심부를 관측할 수 없는 기간에는 초신성, 소행성 및 외부은하 등을 관측한다. 각 관측 프로그램의 시간배정 및 관측결과 요약 등의 정보를 홈페이지에 제공함으로써(http://kmtnet.kasi.re.kr/kmtnet-monitor/) 각 프로그램의 관측 상황을 효율적으로 모니터링 할 수 있도록 지원한다. 이 발표에서는 지난 2015년 우리은하 중심부를 관측하여 얻은 약 31.5TB의 관측 자료 분석 결과를 통해 구한 관측시스템의 성능을 리뷰하고 2016년 관측시스템 운영계획에 대하여 논의한다.

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