• Journal of Hydrogen and New Energy
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• v.28 no.3
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• pp.279-286
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• 2017

Korea government decides to build one hundred hydrogen refueling stations (HRS) until 2020 and tries to disseminate HRS and boosts HRS market in korea. Naepo HRS in chungnam province has been operated for last one full year of 2016 and recorded 2,520 times full charge for the hydrogen fuel cell powered vehicles and total 6,016 kg hydrogen fueling for the 25 units of hydrogen fuel cell powered vehicles. Raw fuel of hydrogen from tube trailer measured by pressure, converting into weight of hydrogen and shows 19.6% surplus with final charged weight by dispenser. This result is caused measuring errors. Measured charged errors between dispenser and Mass flow meter was determined 13.13%.

• Microbiology and Biotechnology Letters
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• v.22 no.6
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• pp.685-691
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• 1994

The biodegradation of aromatic compounds by mixed and monoculture was investigated in an artificial wastewater containing 500 mg/l of benzene(B), phenol(P), and toluene(T) in various combinations. None of three strains utilized P-xylene(X) as a carbon source, but they grew well on p-xylene in mixtures with benzene and toluene. In the mixed culture on mixed substrate, the length of lag phase was different depending on the nature of mixture. Cell growths of Flavobac- terium sp. BEN2 and Acinetobacter sp. GEM63 were inhibited in the presence of a 500 mg/l of phenol. When the mixed culture of three strains was cultured in a bench-scale reactor containing artificial wastewater, each of benzene, phenol, and toluene was not detected at 30 hrs, 50 hrs, and 12 hrs after incubation in the treatment. The removal rates of COD$_{t}$(total COD) and COD$_{s}$,(soluble COD) of upper phase after centrifugation during early 50 hrs were ca. 80% and ca. 93.8%, respectively.

• Journal of Plant Biology
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• v.22 no.4
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• pp.95-100
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• 1979

As a part of the studies on the regulatory mechanism of gene expression by $GA_{3}$ , the effects of $GA_{3}$ on the protein biosynthesis and phosphorylation in maize seedlings were investigated. 1. The optimum concentration of $GA_{3}$ for the stimulation of the protein biosynthesis was 0.3mM. 2. The protein biosynthesis was remarkably increase by $GA_{3}$ during the germination. The reason for the decrease in the protein biosynthesis by 48hrs. after germination seems to be a staggered gene expression, and/or increases in protease and RNase activities. 3. The ratio of the amount of the newly synthesized protein in germinating seeds treated with $GA_{3}$ to the amount of proteins secreted into the endosperm was similar to that ratio in control. According to this result, it seems that $GA_{3}$ stimulates only the expression of certain definite genes. 4. By the treatment with $GA_{3}$, the rates of biosynthesis and phosphorylation of proteins were increased up to about 1.5 times during germination and 6 times by 72hrs. after germination, respectively. The ratio of the total soluble proteins to the phosphorpoteins considerably increased in the early germination stage (24hrs.) but decreased after 24hrs. According to the above mentioned results, the stimulation of the phosphorylation of proteins of $GA_{3}$ seems to be attributed to the increases in the activities of protein kinases.

• The Korean Journal of Malacology
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• v.22 no.2
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• pp.151-155
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• 2006

Proportion of Tegillarca granosa surviving after 2-6 hrs air exposure with 12 hrs interval at $20^{\circ}C$ and $28^{\circ}C$ for 20 days showed 85-100%, 80-100%, respectively. The survival rate was somewhat lower at high temperature but not significant (p < 0.05). Subsequent exposures for 7-9 days showed survival rate of 8.0-24.1% at $20^{\circ}C$ and $28^{\circ}C$. Oxygen consumption rates and filtration rates were significantly higher for 4 to 6 hrs exposures, compared with the preceding exposures. On the other hand, at $28^{\circ}C$, oxygen consumption rates in adult granular ark for 6 hrs exposure during 20 days had significantly decreased. Filtration rates in study groups increased a little over extended period of exposure, compared with those in control groups, and were similar irrelevant to the time of exposure and size of experimental animals. It is concluded, in view of their viability and oxygen consumption rate during air exposure, that ark shells have quite a high resistance to air exposure with their limited range of responses.

• Journal of Pharmaceutical Investigation
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• v.21 no.4
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• pp.247-251
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• 1991

Effects of aluminum magnesium hydroxide (A) and cimetidine (C) on the pharmacokinetics of minocycline (M) were investigated in female rats. Blood samples were collected at various time intervals until 36 hrs following oral dosing of drugs. Plasma minocycline concentrations were determined by HPLC. Control group (M), $T_1$ group (M+A), $T_2$ group (A+M after 2 hrs), $T_3$ group (M+A after 2 hrs), $T_4$ group (M+C) and $T_5$ group (C+M after 2 hrs) were divided to examine interaction of the drugs with minocycline. Plasma minocyline level-time curves were well described by two-compartment open model with first-order absorption in rats. Antacid treatment was associated with reduced of 71.0, 45.9, 35.7% in minocycline absorption rate $constant(K_{\alpha})$, maximum plasma $concentration(C_{max})$, and relative $bioavailability(F_{rel})$, respectively. Cimetidine treatment group exhibited no significant changes in plasma level-time curve when compared with control group and did not affect minocycline absorption as by any of these three parameters.

• Journal of Embryo Transfer
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• v.11 no.2
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• pp.95-101
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• 1996

The present study investigated the effect of treatment with nocodazole on the efficiency of cell cycle synchronization and development of mouse 4-cefl embryos. In addition, developmental ability of reconstituted embryos that received nuclei from 4-cell embryos synchronized with nocodazole at M phase was examined in vitro and in vivo. (1) When 4-cell blastorneres exposed to culture medium containing l$\mu$g /ml nocodazole for 2, 4, 6 and 8 hrs, 40% (40/10l), 75% (l19/159), 85% (87/102) and 97% (155/160) of nuclei were synchronized at M phase, respectively. (2) Treated with nocodazole for 4 hrs, the proportion of 4-cell embryos developed to blastocysts (98%, 60/61) was not significanUy different from that of the control embryos (98%, 196/201). However, the developmental ability of 4-cell embryos treated for 8 (87%, P<0.05)and 12 hrs (76%, P

• Food Science and Preservation
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• v.4 no.1
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• pp.69-75
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• 1997

Peony is a medicinal herb which have utilized widely as chineses medicine. The paeoniflorin is the predominant component In peony root, monoterpene glucoside containing pinane structure. The effective components were extracted with the cold water from the intact peony roots, and effectively extracted with 70% ethanol from the dry powder of peony roots. The changes of paeoniflorin contents were investigated during the drying process of peony roots and processing of peony extract by the heat-treatment. Air-drying was the best condition for the preservation of paeoniflorin content among the drying processes of peony roots. But convective drying at 6$0^{\circ}C$ was recommended for the drying process of peony roots in large scale. The paeoniflorin in peony extracts was not destroyed by the treatment at 6$0^{\circ}C$ and 8$0^{\circ}C$ for 5 hrs, but destroyed 30%, 28% and 40% of paeoniflorin by treatment at 10$0^{\circ}C$ for 5 hrs, 115$^{\circ}C$ and 121$^{\circ}C$ for 10 minutes, respectively. The paeoniflorin was continueously extracted for 4 hrs from the dry pieces of peony roots(0.5$\times$0.5$\times$0.5cm) in boiling water but destroyed gradually after 4 hrs at 10$0^{\circ}C$. Paeoniflorins in 70% ethanol extracts of peony root were not destroyed at all in the process of concentration to dry powder at 60"C on vacuum.cuum.

• YAKHAK HOEJI
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• v.44 no.2
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• pp.189-192
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• 2000

We examined a dermal pharmacokinetics for a new SYC-fentanyl patch in rabbits. Determination of fentanyl in the plasma was performed using a gas chromatography with nitrogen-phosphorus detection system and a capillary column. One patch per animal (fentanyl 2.5 mg) was applied to clipped back skin for 72 hours. The plasma fentanyl concentration profile of SYC-patch was similar to that of a conventional patch (Durogesi $c^{R}$, Janssen Co.). No significant difference was observed in the pharmacokinetic parameters, the area under the concentration-time curve (AU $C^{0-}$72hrs/) and the total area under the first moment-time curve (AUM $C^{0-}$7hrs/), between the two patch types. The AU $C^{0-}$7hrs/ and AUM $C^{0-}$72hrs/ of durogesi $c^{R}$ were 183.3$\pm$46.28 ng＊hr/ml and 6,450$\pm$1,939ng＊h$r^2$/ml, and those of SYC-fentany patch were 217.2$\pm$50.51$\pm$ng＊hr/ml and 8,022$\pm$2,245ng＊h$r^2$/ml, respectively (n=3). This result indicates that the new SYC patch has a similar bioavailability compared to durogesi $c^{R}$ patch. Therefore, the SYC-patch may be considered as a bioequivalent fentanyl patch.patch.tch.

• Journal of Hydrogen and New Energy
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• v.30 no.2
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• pp.163-169
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• 2019

This study is a research to compare efficiency of new cooling system (chiller, pre-cooler) to that of the conventional system at the hydrogen refueling station (HRS). This study includes contents for thermodynamic comparison of cooling system for HRS and comparison of pros and cons of its components. So It is to establish design concept of cooling system of HRS supplying with fuel cell electric vehicle (FCEV). HRS is charging high pressure H2 (700 bar) to FCEV. However cooling system is need to prevent temperature rise in tank. This cooling system consists of pre-cooler and chiller system.

• Progress in Superconductivity and Cryogenics
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• v.11 no.1
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• pp.64-68
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• 2009

To keep the superconducting (SC) magnet coils of KSTAR at proper operating conditions, not only the coils but also other cold components, such as thermal shields (TS), magnet structures, SC bus-lines (BL), and current leads (CL) must be maintained at their respective cryogenic temperatures. A helium refrigeration system (HRS) with an exergetic equivalent cooling power of 9kW at 4.5K without liquid nitrogen $(LN_2)$ pre-cooling has been manufactured and installed for such purposes. In this proceeding, we will present the commissioning and initial operation results of the KSTAR HRS. Circuits which can simulate the thermal loads and pressure drops corresponding to the cooling channels of each cold component of KSTAR have been integrated into the helium distribution system of the HRS. Using those circuits, the performance and the capability of the HRS, to fulfill the mission of establishing the appropriate operating condition for the KSTAR SC magnet coils, have been successfully demonstrated.