• Title/Summary/Keyword: HPLC detector

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Fundamental Studies of the Electrical Characteristics of the Glow Discharge for the Development of HPLC Detector (글로우방전을 이용한 액체크로마토그라피 검출기 개발을 위한 기초특성연구)

  • 이현주;김효진
    • YAKHAK HOEJI
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    • v.39 no.4
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    • pp.427-432
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    • 1995
  • The plasma oscillation has been observed in an argon pressure between a tungsten anode and cathode consisting of an aqueous conducting solution. The effects of experimental parameters on the electrical characteristics of the glow discharge have been studied. The experimental parameters include the anode-cathode distance, pressure, methanol flow rate, and cathode materials. The glow discharge with liquid cathode and solid anode showed the potential sensitive detector for HPLC

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The Use of the Online Two-dimensional Liquid Chromatography Coupled with a Universal Detector for the Screening of Non-volatile Potential Migrants in Food Packaging Materials (식품포장재내 비휘발성 잠재 이행물질들의 스크리닝을 위한 이차원크로마토 그래피와 범용검출기의 이용)

  • Yoon, Chan-Suk;Lee, Keun-Taik
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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    • v.16 no.1
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    • pp.9-18
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    • 2010
  • For screening test of the non-volatile compounds which migrate from food packaging materials into foodstuffs, the traditional high performance liquid chromatography (HPLC) systems suffer from the lack of universal detector with high sensitivity and universality and high efficiency HPLC separation column which provides complete separation of complex mixtures into all individual substances. In this work, the use possibility of online two-dimensional liquid chromatography (2D-LC) system coupled with a charged aerosol detector (CAD), a universal detector, was reviewed. 2D-LC system permits to improve peak capacity and resolving power for complex mixtures. Charged aerosol detector (CAD) offers a new feasibility for detection of any non-volatile compounds with high sensitivity and constant response factor in a calibration range. The combination of size exclusion chromatography (SEC) and normal phase HPLC (NP-HPLC) is most frequently used for the separation of the natural and synthetic polymers which are mainly used as raw materials for the manufacture of food packaging materials. However, there is no commercial software available for data acquisition and handling and therefore the quantification in 2D-LC analysis is still rare.

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Determination of Diamino Diphenyl Sulfone in Vegetable and Fruits by HPLC (HPLC의 Photodiode Array Detector에 의한 과채류중의 Diamino Diphenyl Sulfone 분석)

  • 김명희;명노홍;박성배
    • Journal of Food Hygiene and Safety
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    • v.11 no.2
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    • pp.143-147
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    • 1996
  • This study was carried out to determine and confirm of Dapsone in vegetables and fruits which were illegally used for freshness. We have developed a simple, arpid and precise method that Dapsone can be analyzed in the cabbages, grapes and strawberry by HPLC with photodiode array detector. Experimental subjects were included 15 cases of cabbages, 10 cases of grapes and 10 cases of strawberries purchased in Kangwon, Chungchong province and the Seoul area. The results were obtained that Dapsone in the experimental subject was separated completely within 10 min. Detection limit of Dapsone was 0.5 ng. Aberage recoveries from cabbages, grapes and strawberries were 93.3$\pm$0.37%, 91.4$\pm$0.65% respectively. 4 cases of cabbages were detected Dapsone and the amount was below the 0.3 ppm. There was not detected Dapsone in any grape and strawberry samples.

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Application of Competitive ELISA Method for Estimation of Urinary Aflatoxin M1 Level (ELISA 방법을 이용한 요중 아플라톡신 M1 측정)

  • Kim, Yong-Dae;Kim, Heon
    • Journal of Life Science
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    • v.23 no.2
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    • pp.306-310
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    • 2013
  • We compared the efficacy of the competitive ELISA method for measuring the level of urinary aflatoxin M1 (AFM1) with that of the HPLC-fluorescence detector (HPLC-FLD) method. The recovery rate of AFM1 with the ELISA method was 105% (73-124%), and the coefficient of variation of the analysis was 6.85%. The ELISA method showed a 0.20 pg/ml and 0.62 pg/ml limit of detection and limit of quantitation, respectively. In correlation analysis, the two methods showed a very strong and statistically significant correlation (R=0.96, p<0.01). However, in spite of the strong correlation, the ELISA method tended to overestimate the urinary AFM1 concentration compared to the HPLC-FLD method. These results suggest that the competitive ELISA method may be a useful technique for measuring the AFM1 level in high-throughput urine samples, but it needs to be corrected with a regression equation from regression analysis with the HPLC-FLD method.

Development of Analytical Methods for Micro Levels of Naphthalene and TNT in Groundwater by HPLC-FLD and MSD (HPLC-FLD와 MSD를 이용한 지하수 중 나프탈렌 및 TNT의 미량 분석법 개발)

  • Park, Jong-Sung;Oh, Je-Ill;Jeong, Sang-Jo;Choi, Yoon-Dae;Her, Nam-Guk
    • Journal of Soil and Groundwater Environment
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    • v.14 no.6
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    • pp.35-44
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    • 2009
  • Naphthalene and TNT (2,4,6-trinitrotoluene) are defined by U.S. EPA as possible carcinogenic compounds known to have detrimental effects on the aquatic ecosystem and human body. There are, however, few researches on methods of analyzing micro-levels of naphthalene and TNT dissolved in groundwater. This study introduces and evaluates the newly developed analytical methods of measuring naphthalene and TNT in groundwater by using HPLC-FLD (Fluorescence detector) and MSD (Mass detector). The MDL, LOQ and salt effect of these methods, respectively, are compared with those of conventional methods which use HPLC-UV. For the analysis of naphthalene, HPLC-FLD was set in the maxima wavelength (Ex: 270 nM, Em: 330 nM) obtained from 3D-Fluorescence to be compared with HPLC-UV in 266 nM wavelength. The MDL ($0.3\;{\mu}g/L$) and LOQ ($2.0\;{\mu}g/L$) of naphthalene by using HPLC-FLD were approximately 80 times lower than those analyzed by HPLC-UV (MDL: $23.3\;{\mu}g/L$, LOQ: $163.1\;{\mu}g/L$). HPLC-MSD were used in comparison with HPLC-UV in 230 and 254 nM wavelength for the analysis of TNT. The MDL ($0.13\;{\mu}g/L$) and LOQ ($0.88\;{\mu}g/L$) of TNT analyzed by using HPLC-MSD were approximately 130 times lower than those obtained by using HPLC-UV in 230 nM (MDL: $16.8\;{\mu}g/L$, LOQ: $117.5\;{\mu}g/L$). The chromatogram of TNT analyzed by using HPLC-UV in 230 nM displayed elevated baseline as the concentration of ${NO_3}^-$ increases beyond 21 mg/L, while the analysis using HPLC-MSD did not demonstrate any change in baseline in presence of ${NO_3}^-$ of 63.7 mg/L which is 3.5 times higher than average concentration in groundwater. In conclusion, HPLC-FLD and HPLC-MSD may be used as suitable methods for the analysis of naphthalene and TNT in groundwater and drinking water. These methods can be applied to the monitoring of naphthalene and TNT concentration in groundwater or drinking water.

Utilization of Biomass Resources(I) - HPLC Analysis of Chemical Components for Utilization of Chestnut Inner Bark - (Biomass 자원의 활용 (I) - 율추의 유효이용을 위한 화학적 조성분의 HPLC 분석 -)

  • Kim, Yun-Geun;Jo, Jong-Soo
    • Journal of the Korean Wood Science and Technology
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    • v.32 no.2
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    • pp.58-64
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    • 2004
  • For the utilization of chestnut inner bark as forest biomass, the diethyl ether solubles of hot water extract from chestnut inner bark was analyzed by HPLC. Each peak was identified by comparing with retention time of standard regents and their purity from obtained UV spectrum by RI detector. Identified 6 compounds were gallic acid, 3,5-dihydroxybenzoic acid, 2,4,6-trihydroxybenzoic acid and protocatecualdehyde as phenolic acids and aldehyde, and catechin and epicatechin as flavonoids.

Development of Schiff Base Column and Glow Discharge Detector for HPLC : Preliminary Study I (HPLC용 Schiff Base 컬럼과 Glow Discharge 검출기의 개발에 관한 기초연구 I)

  • Kang, Mi-Ra;Kim, Eun-Soo;Shin, Jung-Sook;Park, Hyun-Kook;Yang, Jung-Sung;Lee, Sang C.
    • Analytical Science and Technology
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    • v.8 no.3
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    • pp.265-272
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    • 1995
  • Schiff base ligand columns and glow discharge detector have been developed for the trace analysis of metal ions desolved in water. Various types of hydrazide Schiff base ligands have been used and, additionally, they were examined as a filling material of a HPLC column. The hydrazide Schiff base ligands used were N, N'-oxalybis(salicylaldehydrazone) (OBSH), N, N'-malonylbis(salicylaldehydrazone) (MBSH), and N, N'-succinylbis(salicylaldehydrazone) (SBSH). A mixture of Schiff base ligand and poly(styrene divinylbenzene) was examined and it showed a smooth flow of solution. The OBSH-polymer column demonstrated different effluent factors for different metal ions. Metal ions in eluates were detected by Hollow Cathode Glow Discharge-Atomic Emission Spectrometry(HCGD-AES). HCGD-AES showed good sensitivity and selectivity. This is only the preliminary results of new OBSH-polymer column and glow discharge detector.

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Simultaneous determination of sunscreen agents in cosmetics by HPLC (HPLC를 이용한 화장품 중의 자외선 차단제 동시분석방법 연구)

  • Lee, Yong-Hwa;Yang, Jae-Chan
    • Journal of the Korean Applied Science and Technology
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    • v.29 no.4
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    • pp.577-584
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    • 2012
  • The simultaneous analysis of sun screen agents in commercial cosmetic samples was carried out by High Perfomance Liquid Chromatography(HPLC). The cosmetic samples are directly dissolved in Tetrahydrofurane(THF) and filtered using $0.45{\mu}m$ filter. The water/methanol/THF was used for the mobile phase of gradient conditions. An Extend C18 reversed-phase column and the selected UV/Visible detector was applied. The analysis results of HPLC showed good linearity with correlation coefficient of $r^2$=0.9992 in the rage of $50{\sim}800{\mu}g/mL$ and detection limit of $0.01{\mu}g/mL$.

Extraction Methods and HPLC Analysis Conditions of Paeoniflorin in Peony, Paeonia lactiflora Pall. (작약(Paeonia lactiflora Pall.)에서 paeoniflorin 추출방법 및 HPLC 분석조건)

  • ;Kwang-He Kang
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.39 no.6
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    • pp.542-547
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    • 1994
  • To find out the most reasonable analysis conditions of paeoniflorin, different paeoniflorin extraction methods and various UV detector wavelengths were conducted with paeonia radix of 4-year old Euisung local variety. The most reasonable paeoniflorin extraction time by reflux apparatus was 1hr. and by ultrasonic apparatus was 3hrs. and those methods were completed only once. Concentration of paeoniflorin by reflux apparatuses at 1hr. and 2hrs. of extracting time were higher than those of ultrasonic apparatus, and the differences were highly significant. However, the differences of paeoniflorin concentration at 3hrs. and 4hrs. in two methods were not significant. In comparing paeoniflorin concentration of many lines, ultrasonic extracting apparatus was more simple and effective than the reflux apparatus. Paeoniflorin was more reasonable sensitivity at 240nm, and albiflorin was 254nm by HPLC. When paeoniflorin and albiflorin were analyzed simultaneously, 254nm was more stable than any other wavelength.

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