Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Simultaneous determination of sunscreen agents in cosmetics by HPLC

HPLC를 이용한 화장품 중의 자외선 차단제 동시분석방법 연구

  • Lee, Yong-Hwa (Hoseo University, Fusion technology) ;
  • Yang, Jae-Chan (Mokwon University, College of Sciences & Technology, Division of Biomedicinal & Cosmetics)
  • 이용화 (호서대학교 융합기술연구소) ;
  • 양재찬 (목원대학교 테크노과학대학 생의약화장품학부)
  • Received : 2012.11.19
  • Accepted : 2012.12.16
  • Published : 2012.12.30
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Abstract

The simultaneous analysis of sun screen agents in commercial cosmetic samples was carried out by High Perfomance Liquid Chromatography(HPLC). The cosmetic samples are directly dissolved in Tetrahydrofurane(THF) and filtered using $0.45{\mu}m$ filter. The water/methanol/THF was used for the mobile phase of gradient conditions. An Extend C18 reversed-phase column and the selected UV/Visible detector was applied. The analysis results of HPLC showed good linearity with correlation coefficient of $r^2$=0.9992 in the rage of $50{\sim}800{\mu}g/mL$ and detection limit of $0.01{\mu}g/mL$.

액체크로마토그래피법(HPLC)을 이용하여 화장품에 들어있는 자외선차단제류 등을 동시 분석하였다. 화장품 시료를 Tetrahydrofurane(THF)에 직접 용해시키고 $0.45{\mu}m$ 필터로 여과하여 물/메탄올/THF를 이동상으로 하여 Extend C18의 비극성 컬럼을 사용하여 기울기용리조건에서 20분 안에 분리하여 UV/Vis detector방법으로 정량하였다. HPLC분석결과 검량선은 $50{\sim}800{\mu}g/mL$ 농도범위에서 상관계수가 $r^2$=0.9992 이상의 좋은 직선성을 나타내었으며 검출한계는 $0.01{\mu}g/mL$였다.

Keywords

References

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