• Korean Journal of Microbiology
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• v.41 no.4
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• pp.239-245
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• 2005

Transformation-Associated Recombination (TAR) cloning technique allows selective isolation of chromosomal regions and genes from complex genomes. The procedure requires knowledge of relatively small genomic sequences that reside adjacent to the chromosomal region of interest. This technique involves homologous recombination during yeast spheroplast transformation between genomic DNA and a TAR vector that has 5' and 3' gene targeting sequences. In this study, we examined the effect of non-homologous spacing sequence in target hooks on homologous recombination using a plasmid model system. The efficiency of homologous recombination between the modified his3-TRP1-his3 fragments and HlS3 gene on plasmid were analyzed by the characterization of $Ura^+$ transformants. The numbers of $Ura^+$ transformant showed same level when seven different modified his3-TRP1-his3 fragments were used. But the percentage of positive recombinants. $Trp^+His^-$, dramatically decreased when used the modified his3-TRP1-his3 fragments contained incorrect spacing of nonhomologous region. As a result, we suggest that incorrect spacing inhibits the homologous recombination between target hook and substrate DNA. Therefore, we should consider the correct spacing in target hook when the target hook are used for cloning of orthologue gene.

• (The)Study of the Eastern Classic
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• no.33
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• pp.57-96
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• 2008

Kim, Kyung Jang(1597~1653) as a disciple of Jang Hyun Gwang was very famous for his virtuous conduct. But his collection of works has been published recently. There has never been any studies about his thoughts, learning and life. Therefore this study has a target to arrange the process of his life and events, and to introduce outlines of his learning. The items of this study will be as follows: The first category will be his lineage, his life, and writings. The second category will be his learning. This category has two lower categories. The first will be focused on his intention to morally accomplished self by the method of self cultivation and practise of Confucian proprieties. The second will be focused on his learning of the Change and of the Confucian proprieties. And giving attention to his 6 diagrams that have symbolized principles of the Change, this paper tries to compare these to the other scholars ones and so will explane the features of his diagrams. The most important thing in this paper is to define his learning as so called in the rhetoric of Confucianism Wi-gi-ji-hak(爲己之學), which means self organization of one's moral personality by the method of self cultivation and to distinguish the steps of his learning developed. This method has never been applied, but this is expected to become a effective method for explanation of the truth of the Confucian scholar's world of learning.

• Korean Security Journal
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• no.4
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• pp.189-218
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• 2001

Mr. Cho OO, a famous ex-delinquent who has already spent 27 years in prison for his hardened stealing habit and escape, was recently again arrested by Japanese police for housrbreaking in Japan. Every Korean people would have believed that he had abandoned his habit for good, because, since his liberation in 1998 , he showed himself as a man perfectly cured. He became a believer more than ever, married a well-to-do beautiful woman, got a son from her, had a good income from his job as homme security counsellor and from several other occupations, and after all worked a lot for homeless and unfortunate. Now, his case is given as an important subject to approach for criminologists. As well as we are concerned, we thought, to explain his case criminological, it's primarily necessary to skim through the main criminological theories, and that in behalf of this case. After that, we will be able to decide which theory is mostly appropriate to explain his inveterate disease. The one we chose is the social learning theory. But this theory alone won't allow us to understand all aspects of his crime. This is why we will have to turn to several other theories to complete our explanation. But theoretical understanding alone of a criminal case is useless if it cannot suggest a remedy for it. And, we finish our study by making some suggestions on this case.

• Korean Institute of Interior Design Journal
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• v.25 no.1
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• pp.13-23
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• 2016

2014 Venice Biennale of Architecture, presented by general director Rem Koolhaas, has shown the distinct difference between his present displays and past displays. Under the theme of FUNDAMENTALS and ABSORBING MODERNITY, Rem Koolhaas has developed his own unique way which is a research-oriented display technique to explain the difference at the exhibition. The purpose of this study is to define a research-oriented display technique as realism rhetoric for the opened dialectic and identify its aspect of a methodic approach. This report investigated not only Rem Koolhaas's thought and theoretical approach through his work but also the comparison of between his present and past displays. His display is based on the constellation of Central Pavilion, National Pavilions, and Arsenale display and reflected the reality of architecture in self-deception through the metonymical interaction of various facts and records. Rem Koolhaas called it the composition of contradiction. In his work, the spatial situation which is the present has been made with the intervention of historical events and has revived the reality of architecture. Also, the present is a montage of the strict control of architecture, the isolation from the architecture itself, and the autonomic communication with other fields. Finally, as a result of his work, Rem Koolhaas has shown that his display is not the end of phase but a phase of creation.

• Korean Journal of Applied Biomechanics
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• v.13 no.3
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• pp.15-26
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• 2003

This study was conducted to investigate the performance times, CM position and CM speed, pole chord length and pole chord angle, whole body angular momentum(X axis), and grip width in pole vault event according to the event and phase; touch down, pole plant, take-off, maximum pole bending pole straight, pole release, peak height, and foot contact, pole contact, free flight. The pole vaulting of four male elite vaulters including six trial were filmed using two video digital cameras at 60 Hz at 56th national athletic match, and data were collected through the DLT method of three dimensional cinematography. In general the better jumper is, the longer the performance time is. And the greater CM speed is, and the better his transformation ability of CM horizontal speed into vertical speed is. As he uses a longer pole, his grip is higher, and it is a enough for him to rock back his body, so that he pulls and pushes the pole well keeping his hips close to. An greater maximum angular momentum and early positioning of the hips parallel to the bar makes his body far side of the bar and his bar clearance easier. Specially our national jumper needs to have more powerful braking force during foot contact phase, and take his body on the pole after maximum pole bending, and pull and push the pole strongly keeping his hips close to. Also he needs to have stronger muscular strength in order to control the longer pole and use the pole of proper tension more efficiently.

• Journal of the Korean Institute of Rural Architecture
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• v.22 no.3
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• pp.25-32
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• 2020

The purpose of this study is to understand what Le Corbuiser has intended on a panoramic landscape view through his modern architectural design. This paper is willing to improve that he considered both natural and local landscape scenery, when he designed architecture with drawing images. He designed various ways to see outside scenery and community culture through ribbon windows, piloti, architectural promenade, picture frame, and rooftop garden as the angle of view inside the building, 'designed landscape panorama' from his architecture. Therefore the contents of the study include the analysis of the local landscapes shown through his architecture by photograph, drawing of a real scenery, and his sketches with biology to find what he has intended. The following conclusions have three points. First, Le Corbuiser has a basic idea to bear a natural and local scenery from his architecture through five points of new architecture. Second, pilotis, ribbon windows, and roof garden with picture frame and architectural promenade are pathways of his architecture to see 'designed landscape panorama'. Third, it comes from his early architecture like Villa Savoye in 1920s to Couvent de Sainte Marie de la Tourette in 1950s, but Ronchamp church converts his idea on previous thoughts.

• Journal of The Korean Digital Architecture Interior Association
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• v.13 no.4
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• pp.13-23
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• 2013

Steven Holl is outstanding architect who has been applied his phenomenological thinking to his works. he has been reported that he had adhered to focus his interest on coherent phenomenological theme and sublimated his architectural concept succesfully. This study focuses on relationship how phenomenological concept has common ground with architectural parts and with the study on phenomenological application methodology of architectonics of Steven Holl. In the case of Holl, with the theme of sense and flesh, he molds phenomenologically sensitive space. His works contains coherent phenomenolgical concepts such as distrust of rationality, synthesis of consciousness, and qeustions of perception. The Methodologies of connection with environment(nature or bridge), regional values, placeness, light and time are considered in his works(books and architectural works) as anchoring, interwining and parallax for phenomenological process in his concepts. His methodologies of enlisted works contain many valuable concepts of phenomenological intuition and would be applicable to contemporary and future architecture for humanism.

• Journal of Korean Medical classics
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• v.23 no.3
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• pp.49-67
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• 2010

The actual name of Gogobong is Du-goe, also known as Dan-jung, his pseudonym. He is the author of "Uigasimbeop(醫家心法)", an abstract of his clinical pathology throughout his life and "Chwimopyeon(吹毛編)", a medical chart based on his researches. The chapter 25 Bangrons[二十五方論] is included within the text, which shows attempts on explaining diseases and treatments of the internal organs based on theories on the relations of generation and restriction of the five elements. Influenced by Joheonga(趙獻可), one of the well known Onbo(溫補) scholars-concerning the importance of warming and invigorating the body-of the Myeong-dynasty, his main treatments were forms of warming and invigorating the yang and physical debility, hence strengthen the primordial energy. "Uigasimbeop(醫家心法)" was later on renamed to "Samyeongsimbeop(四明心法)" by Yangseungryuk(楊乘六). The following paper deals on his life, publishment, distinct features of his medical science based on his epitaph and works.

• Archives of design research
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• v.16 no.1
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• pp.199-208
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• 2003

The Israel-born Ron Arad is one of Britain's "superstar" designers, an it has been said that he is Britain's answer to Philippe Starck. Arad is a highly individualistic designer whose ideas stem from a mental process that has more in common with that of the fine artist than of the jobbing designer for industry. His imagination expresses itself in the form of things. By the early 1990s he had become internationally recognized for his "ready-made" work. His furniture and lighting designs required extremely costly labour-intensive techniques to produce. As his work was highly evocative, suggesting a post-industrial world in which the urban landscape was characterized by materials in a state of decay. More recently, through collaborations with Italian and German manufactures, his work has reached a new level of sophistication and finish, which differentiates it from the earlier designs. As Arad reputation as a designer of workshop-based furniture came to an end and the era of "democratic" Arad furniture came into being. By middle of the decade he had become one of the most-discussed designers of the day. The spirituality that emanates from all his work is a product of his particular vision of the creative process, far removed from the typical, stereotyped trends in the sphere of modern industrialization. He goes one step further in his work by attempting to restore aesthetic dignity to the objects of daily life, in a search for beauty within the immediate environment.hin the immediate environment.

• Genomics & Informatics
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• v.4 no.3
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• pp.133-136
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• 2006

The adsorption of proteins on the surface of glass slides is essential for construction of protein chips. Previously, we prepared a nickel-coated plate by the spin-coating method for immobilization of His-tagged proteins. In order to know whether the structural factor is responsible for the immobilization of His-tagged proteins to the nickel-coated glass slide, we executed a series of experiments. First we purified a His-tagged protein after expressing the vector in E. coli BL21 (DE3). Then we obtained the unfolding curve for the His-tagged protein by using guanidine hydrochloride. Fractions unfolded were monitored by internal fluorescence spectroscopy. The ${\Delta}G_{H20}$ for unfolding was $2.27kcal/mol{/pm}0.52$. Then we tested if unfolded His-tagged proteins can be adsorbed to the nickel-coated plate, comparing with $Ni^{2+}-NTA$ (nitrilotriacetic acid) beads. Whereas unfolded His-tagged proteins were adsorbed to $Ni^{2+}-NTA$ beads, they did not bind to the nickel-coated plate. In conclusion, a structural factor is likely to be an important factor for constructing the protein chips, when His-tagged proteins will immobilize to the nickel-coated slides.