• Title/Summary/Keyword: HIGHT

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Detection of Fusarium Species by Enzyme-Linked Immunosorbent Assay Using Monoclonal Antibody

  • Kwak, Bo-Yeon;Kwon, Byung-Joon;Kweon, Chang-Hee;Shon, Dong-Hwa
    • Journal of Microbiology and Biotechnology
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    • v.13 no.5
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    • pp.794-799
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    • 2003
  • Enzyme-linked immunosorbent assay (ELISA) was developed for the rapid detection of Fusarium species, known as harmful fungi in food. One of the hybridoma cell lines (lB8) which produced a monoclonal antibody (Mab) specific to Fusarium extracellular polysaccharide (EPS) was screened and the Mab was produced and purified. A detection limit of the sandwich ELISA against F. moniliforme EPS was $0.001\;\mu\textrm{g}/ml$ in the standard curve. Among the 59 strains tested, most Fusarium species showed hight reactivity with Mab lB8, even when the culture broths were diluted 100,000 times. On the other hand, the other genera, except A. versicolor and Trichoderma viride, had no reactivity. When 1 to $100\;\mu\textrm{g}$ of F. moniliforme EPS was spiked into rice, potato, and mandarine orange, the average recoveries were 151%, 84%, and 94%, respectively, determined by sandwich ELISA. The correlation coefficients between the EPS levels determined by sandwich ELISA and the dry mycelial weight of the liquid culture of F. moniliforme, as well as between the EPS and colony forming unit in solid culture of potato, were 0.97 and 0.91, respectively.

Studies of Nutrient Intake, Life Style, and Serum Lipids Level in Middle-aged Men in Taegu (대구지역 중년 남성의 영양섭취 상태와 생활습관 및 혈청지질에 관한 연구)

  • 정윤정
    • Journal of Nutrition and Health
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    • v.30 no.3
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    • pp.277-285
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    • 1997
  • The purpose of this study was to investigate the relationship among nutrient intake, life style, and serum lipids level in 108 healthy middle-aged men in Taegu. A convenient method was to assess nutritional intake. Anthropometric measuement of body weight and hight were measured and average energy expenditure was calculated. The mean body mass index(BMI) was 22.8$\pm$2.4 and it was in the middle of the mean BMI of Korean men. Obesity rate of study subject were 13.2%. Daily energy intake was not sufficient as 88.4% of recommended dietary allowances and the energy percentage of carboydrate, fat protein was 65 : 21 : 14. Mean intake of vitamin A, B1, C and Ca were lower than RDA. There was a highly significant negative correlation between the systolic blood pressure and calcium intake(r=-0.28, p<0.05). Smokers showed significantly higher blood glucose than non-smokers. Skipping meals and uneven diurnal distribution(no breakfast and large evening meals) are associated with high triglyceride level in this population. There was a highly significant correlation between body weight and plasma lipids. Energy expenditure was negatively correlated with plasma triglyceride level. Especially, atherogenic index was significantly lower in job-time physically active worker than that in sedentary worker. Above data provides valuable imformation to the community for program planning as well as to health providers who work individual male adults to meet their nutrition needs and to control blood lipids.

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Comparison of Mechanical Properties and Microstructural Charateristies of Tandem GMAW Weld Metal in 490MPa Grade Steel (490MPa급 고장력강 탄뎀 가스메탈아크 용접부에 대한 기계적 성질과 미세조직 비교)

  • Yi, Hui-Jun;Kang, Sung-Soo;Yu, Gum-Bin;Bae, Won-Hak;Moon, Hyun-Soo
    • Journal of Welding and Joining
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    • v.27 no.2
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    • pp.76-81
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    • 2009
  • Tandem GMAW is one of the high performance welding process and used in many industries to increase the productivity. An evaluation is presented of the mechanical properties and microstructural characteristics of the Tandem GMAW and conventional Single GMAW welds in 30mm thickness 490MPa grade steel by comparison method. Welding sequence and bead with and hight was kept, avoiding the effect of the bead shape and welding sequence. Tension, bending, hardness and Charpy impact test results of Tandem GMAW met the requirement of specification and showed similar distribution with conventional Single GMAW. Volume fraction of ferrite phase in weld metal showed little difference between Tandem GMAW and Single GMAW

An Estimation of Extreme Wind Speed of Typhoon Affecting the Damage of Public and Industrial Facilities (공공 및 산업시설 피해에 영향을 미치는 태풍의 최대풍속 도출)

  • Jung, Woo-Sik
    • Journal of Environmental Science International
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    • v.24 no.9
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    • pp.1199-1210
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    • 2015
  • There were 35 typhoons affecting Korean Peninsula from 1999 to 2009(The average annual number of typhoon is 3.18). Among these typhoons, the number of typhoon passing through the Yellow sea, the Southern sea and the East sea were 14, 6 and 15 respectively. Wind speed on the height of 10 m can be finally estimated using the surface roughness after we calculate wind speed on the height of 300 m from the data on the surface of 700 hPa. From the wind speeds on the height of 10 m, we can understand the regional distributions of strong wind speed are very different according to the typhoon tracks. Wind speed range showing the highest frequency is 10~20 m/s(45.69%), below 10 m/s(30.72%) and 20~30 m/s(17.31%) in high order. From the analysis of the wind speed on the hight of 80 m, we can know the number of occurrence of wind speed between 50 and 60 m/s that can affect wind power generation are 104(0.57%) and those of between 60 and 70 m/s that can be considered as extreme wind speed are even 8(0.04%).

Seismic Behavior by Beams-Wall Connections depending upon Joint Details of Embedded Steel-Plate Composite Coupling Beams (Steel-Plate 매입형 합성 연결보의 접합부 상세에 따른 내진거동)

  • Joo, Jae-Hyung;Chea, Seong-Yun;Kim, Seon-Gyu;Kim, Woo-Jae;Jun, Ho-Min;Park, Hyo-Seon
    • Proceedings of the Korea Concrete Institute Conference
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    • 2006.05a
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    • pp.130-133
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    • 2006
  • Owing to Higher story tendency and ceiling hight restriction of recent times, even though the depth of Coupling Beam decreases the demand efficiency is coming to be high rather so it is the difficult to satisfy a demand efficiency with the actual existing RC/Steel condition. It has been researched a lot of methods as an alternative plan about these but designer cannot apply them for the reason ; the structure standard and theory background are not taking a triangular position. As accomplishing the test research about the beams-wall connections department of steel-plate composite coupling beams from the research in the study it examines closely the beams-wall connections conduct quality and tries to provide the fundamental data for reasonable and optimum connection department designs. It knows that connection fixation degree and composite conduct degree of member part together become the important variable showing an energy dissipation ability in Test result.

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A 6.4-Gb/s/channel Asymmetric 4-PAM Transceiver for Memory Interface

  • Lee, Kwang-Hun;Jang, Young-Chan
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2011.05a
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    • pp.129-131
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    • 2011
  • An 6.4-Gb/s/channel 4-PAM transceiver is designed for a high speed memory application. The asymmetric 4-PAM signaling scheme is proposed to increase the voltage and time margins, and reduces the reference noise effect in a receiver by 33%. To reduce ISI in a channel, 1-tap pre-emphasis of a transmitter is used. The proposed asymmetric 4-PAM transceiver was implemented by using 0.13um 1-poly 6-metal CMOS process with 1.2V supply. The active area and power consumption of 1-charmel transceiver including a PLL are $0.294um^2$ and 6mW, respectively.

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A Hight Tilted OCB(HTOCB) Mode using Control of Tilt Angle for Hematic Liquid Crystal on Polyimide Surface (폴리이미드 표면에서의 네마틱 액정의 틸트 제어를 이용한 High Tilted OCB(HTOCB) 모드)

  • Hwang, Jeoung-Yeon;Jeong, Youn-Hak;Seo, Dae-Shik
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.18 no.7
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    • pp.635-640
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    • 2005
  • In this paper, we have improved a novel high tilted optically compensated bend (OCB) (HTOCB) mode by using high tilt angle that was generated by the unique baking condition on the homeotropic alignment layer. The high tilt angle of liquid crystal (LC) was generated by new alignment process that tilt angle changed homeotropic state to homeogenous state using Hot-plate equipment; we obtained about $40\~50^{\circ}$ tilt angle with negative and positive dielectric anisotropy on the homeotropic polyimide (PI), and then LC tilt angle decreased as increasing baking temperature and time. At last, we obtained about $10^{\circ}$ with positive type NLC $({\Delta}n>0)$. Also, the LC tilt angle of positive type NLC $({\Delta}n>0)$ decreased as increasing rubbing strength at the same baking temperature and time. The novel LC operating mode (HTOCB) that used the high tilt angle by the new alignment method was improved. The response time of the novel HTOCB cell was faster than that of conventional OCB cell. We suggest that the developed the novel HTOCB cell using control of tilt angle on the homeotropic surface is a promising technique for the achievement of a fast response time and a high contrast ratio.

A Study on the Costume of Knight in the Cinema with the Background of the Middle Age -Focused on the 11$\sim$15th Century- (중세배경 영화에 나타난 기사복에 관한 연구 -l1$\sim$15세기를 중심으로-)

  • Kim, Hee-Jung
    • Journal of the Korea Fashion and Costume Design Association
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    • v.9 no.1
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    • pp.71-87
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    • 2007
  • Main role to expand the fashion in the middle age was played by aristocracy and knight. They contributed to the fashion of the middle age regardless of aesthetic sense. First, in any age, there was a self-display design using splendid decoration or material in the costume of high-class people. Aristocracy, especially, knight in the middle age classified themselves from others by wearing the costume suitable for their statuses. Second, the Crusade knight appearing all over the cinema gathered various peoples and caused acute optic angle about specialty of costume. Therefore the trend preferring foreign costumes was changed to new fashion while it was connected to desire searching for a change. Third, although armor of knight was created because of protection in the war, in the 13th century, its character of motion and defense was emphasized and it became splendid style. More over, the costume of knight was developed to more emphasize masculine beauty with heavy upper body and light lower body so it caused sexual difference of costume in the middle age. Fourth, As knight searched effective defensive weapon, chain mail was relegated by plate armor. The order of armor that the hight wore in 1350 was as follows. First, he wears adhesive shirts, braies, hose and wears metal protector on arm and leg. He wears padded undercoat called gambeson, hauberk, plate armor and surcoat on them.

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Structural Durability Analysis According to the Thickness of Bicycle Frame Tube (자전거 프레임 튜브 두께에 따른 구조적 내구성 해석)

  • Cho, Jae-Ung;Han, Moon-Sik
    • Journal of the Korean Society of Manufacturing Process Engineers
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    • v.11 no.3
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    • pp.122-129
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    • 2012
  • This study investigates structural and vibration analyses according to the thickness of bicycle frame tube. The model of bicycle frame has the dimension as length of 862mm, width of 100mm and hight of 402.5mm. There are 3 kinds of models with tubes of top, down and seat at bicycle frame as thicknesses of 10, 15 and 20mm. The maximum displacement and stress occur at the center part of seat stay and at the installation part of rear wheel respectively. Maximum displacements become 0.031936, 0.029159 and 0.027984mm in cases of thicknesses of 10, 15 and 20mm respectively. In case of thickness of 20mm among 3 cases, maximum displacement becomes lowest. But maximum stresses become 10.019, 8.5492 and 9.2511MPa in cases of thicknesses of 10, 15 and 20mm respectively. In case of thickness of 15mm among 3 cases, maximum stress becomes lowest. There is no resonance at practical driving conditions and natural frequency remains almost unchanged along the change of thickness. In case of the displacement due to vibration mode, the displacement difference at thickness between 15mm and 20mm becomes 1/2 times than that between 10mm and 15mm. Design at bicycle frame tube becomes most economical and durable effectively in case of thickness of 15mm among 3 cases.

Effect of MS Medium Strength and Nitrogen Concentration on Bulblet Formation and Growth of Lilium In Vitro (MS 배지 내 무기물 농도 및 질소 농도가 나리의 자구 형성과 비대에 미치는 영향)

  • 염미란;박현춘;양찬석;백기엽
    • Korean Journal of Plant Tissue Culture
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    • v.28 no.6
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    • pp.341-346
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    • 2001
  • To investigate the effects of MS medium strength and nitrogen concentration on bulblet formation from bulblet scale segment culture and bulblet growth from bulblet of Lilium oriental hybrid 'Casa Blanca', asiatic hybrid 'Mona', and longiflorum hybrid 'Hinomoto', 0.5∼2.0 strength of MS salts and 30∼120 mM nitrogen concentrations of MS medium were examined in vitro. The number of bulblets from bulb scale segment was favored in the strength of 0.5∼1.0 strength of MS salts or 30 mM total nitrogen concentration of MS medium in three cultivars. But the growth of bulblets formed in vitro was promoted in the 2 strength of MS medium or hight concentration of total nitrogen of MS medium up to 120 mM in three experimented cultivars.

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