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Interaction of Porcine Myofibrillar Proteins and Various Gelatins: Impacts on Gel Properties

  • Noh, Sin-Woo;Song, Dong-Heon;Ham, Youn-Kyung;Kim, Tae-Kyung;Choi, Yun-Sang;Kim, Hyun-Wook
    • Food Science of Animal Resources
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    • v.39 no.2
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    • pp.229-239
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    • 2019
  • The objectives of this study were to determine the interaction between porcine myofibrillar proteins and various gelatins (bovine hide, porcine skin, fish skin, and duck skin gelatins) and their impacts on gel properties of porcine myofibrillar proteins. Porcine myofibrillar protein was isolated from pork loin muscle (M. longissimus dorsi thoracis et lumborum). Control was prepared with only myofibrillar protein (60 mg/mL), and gelatin treatments were formulated with myofibrillar protein and each gelatin (9:1) at the same protein concentration. The myofibrillar protein-gelatin mixtures were heated from $10^{\circ}C$ to $75^{\circ}C$ ($2^{\circ}C/min$). Little to no impacts of gelatin addition on pH value and color characteristics of heat-induced myofibrillar protein gels were observed (p>0.05). The addition of gelatin slightly decreased cooking yield of heat-induced myofibrillar protein gels, but the gels showed lower centrifugal weight loss compared to control (p<0.05). The addition of gelatin significantly decreased hardness, cohesiveness, gumminess, and chewiness of heat-induced myofibrillar gels. Further, sodium dodecyl poly-acrylamide gel electrophoresis (SDS-PAGE) showed no interaction between myofibrillar proteins and gelatin under non-thermal conditions. Only a slight change in the endothermic peak (probably myosin) of myofibrillar protein-gelatin mixtures was found. The results of this study show that the addition of gelatin attenuated the water-holding capacity and textural properties of heat-induced myofibrillar protein gel. Thus, it could be suggested that well-known positive impacts of gelatin on quality characteristics of processed meat products may be largely affected by the functional properties of gelatin per se, rather than its interaction with myofibrillar proteins.

A Method for Original IP Detection of VPN Accessor (VPN 접속자의 원점 IP 탐지 방법)

  • Kim, Inhwan;Kim, Dukyun;Cho, Sungkuk;Jeon, Byungkook
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.21 no.3
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    • pp.91-98
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    • 2021
  • In most hacking attacks, hackers tend to access target systems in a variety of circumvent connection methods to hide their original IP. Therefore, finding the attacker's IP(Internet Protocol) from the defender's point of view is one of important issue to recognize hackers. If an attacker uses a proxy, original IP can be obtained through a program other than web browser in attacker's computer. Unfortunately, this method has no effect on the connection through VPN(Virtual Private Network), because VPN affects all applications. In an academic domain, various IP traceback methods using network equipments such as routers have been studied, but it is very difficult to be realized due to various problems including standardization and privacy. To overcome this limitation, this paper proposes a practical way to use client's network configuration temporarily until it can detect original IP. The proposed method does not only restrict usage of network, but also does not violate any privacy. We implemented and verified the proposed method in real internet with various VPN tools.

An Enhanced method for detecting obfuscated Javascript Malware using automated Deobfuscation (난독화된 자바스크립트의 자동 복호화를 통한 악성코드의 효율적인 탐지 방안 연구)

  • Ji, Sun-Ho;Kim, Huy-Kang
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.22 no.4
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    • pp.869-882
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    • 2012
  • With the growth of Web services and the development of web exploit toolkits, web-based malware has increased dramatically. Using Javascript Obfuscation, recent web-based malware hide a malicious URL and the exploit code. Thus, pattern matching for network intrusion detection systems has difficulty of detecting malware. Though various methods have proposed to detect Javascript malware on a users' web browser, the overall detection is needed to counter advanced attacks such as APTs(Advanced Persistent Treats), aimed at penetration into a certain an organization's intranet. To overcome the limitation of previous pattern matching for network intrusion detection systems, a novel deobfuscating method to handle obfuscated Javascript is needed. In this paper, we propose a framework for effective hidden malware detection through an automated deobfuscation regardless of advanced obfuscation techniques with overriding JavaScript functions and a separate JavaScript interpreter through to improve jsunpack-n.

Proposal of finger splint design using design guidelines to reflect user requirements - Using FDM 3D printing technology - (사용자의 요구조건을 반영 할 수 있는 디자인 가이드라인을 이용한 손가락 보조기 디자인 제안 - FDM 방식의 3D 프린팅 기술을 이용하여 -)

  • Shin, I Yeol;Oh, Kwang Myung
    • Design Convergence Study
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    • v.18 no.3
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    • pp.1-14
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    • 2019
  • General finger splint manufactured and sold domestically could have been of great help to patients with disabilities due to damage to the body's. However, it reminded the wearer of his disability that he wanted to hide. This has had a negative effect on the psychological side of self-absorption and depression. If this avoids or rejects wearing, the role of ancillary rehabilitation is lost. This does not meet the user's requirements. Thus, in this study, 3D printing was used to better reflect user requirements. Next, the study examined existing prior studies to identify the characteristics and criteria of each study. It also examined medical finger aids that were being sold in the auxiliary device market. The assessment criteria were derived by compiling and interpreting user surveys of each finger splint device. Based on the evaluation criteria derived, the design guidelines for finger splint were presented using FDM-style 3D printers. Finally, we proposed a finger splint design according to the proposed design guideline.

Anterolateral thigh free flaps and radial forearm free flaps in head and neck reconstruction: A 20-year analysis from a single institution

  • Yang, Simon;Hong, Jong Won;Yoon, In Sik;Lew, Dae Hyun;Roh, Tai Suk;Lee, Won Jai
    • Archives of Plastic Surgery
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    • v.48 no.1
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    • pp.49-54
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    • 2021
  • Background Reconstruction after removal of a malignant tumor in the head and neck region is crucial for restoring tissue integrity, function, and aesthetics. We retrospectively analyzed patients who underwent intraoral reconstruction surgery using radial forearm free flaps (RFFF) and anterolateral thigh free flaps (ALT) at a single institution to provide more information supporting the choice of a reconstruction method after removal of head and neck cancer. Methods The charts of 708 patients who underwent head and neck reconstruction between 1998 and 2018 at the Department of Plastic and Reconstructive Surgery at our institution were retrospectively reviewed. Patients' age, sex, and history of radiation therapy, diabetes mellitus, and smoking were retrieved. The primary cancer site, types of defects, and complications were investigated. Results Overall, 473 and 95 patients underwent reconstruction surgery with RFFF and ALT, respectively. RFFF was more often used in patients with cancers of the pharynx, larynx, esophagus, or tonsil, while ALT was more frequently used in patients with cancers of the mouth floor with tonsil or tongue involvement. The proportion of patients undergoing ALT increased gradually. Flap failure and donor site morbidities did not show significant differences between the two groups. Conclusions RFFF and ALT flaps resulted in similar outcomes in terms of flap survival and donor site morbidity. ALT can be an option for head and neck reconstruction surgery in patients with large and complex defects or for young patients who want to hide their donor site scars.

Exoproduction and Biochemical Characterization of a Novel Serine Protease from Ornithinibacillus caprae L9T with Hide-Dehairing Activity

  • Li, Xiaoguang;Zhang, Qian;Gan, Longzhan;Jiang, Guangyang;Tian, Yongqiang;Shi, Bi
    • Journal of Microbiology and Biotechnology
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    • v.32 no.1
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    • pp.99-109
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    • 2022
  • This study is the first report on production and characterization of the enzyme from an Ornithinibacillus species. A 4.2-fold increase in the extracellular protease (called L9T) production from Ornithinibacillus caprae L9T was achieved through the one-factor-at-a-time approach and response surface methodological optimization. L9T protease exhibited a unique protein band with a mass of 25.9 kDa upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis. This novel protease was active over a range of pH (4-13), temperatures (30-80℃) and salt concentrations (0-220 g/l), with the maximal activity observed at pH 7, 70℃ and 20 g/l NaCl. Proteolytic activity was upgraded in the presence of Ag+, Ca2+ and Sr2+, but was totally suppressed by 5 mM phenylmethylsulfonyl fluoride, which suggests that this enzyme belongs to the serine protease family. L9T protease was resistant to certain common organic solvents and surfactants; particularly, 5 mM Tween 20 and Tween 80 improved the activity by 63 and 15%, respectively. More importantly, L9T protease was found to be effective in dehairing of goatskins, cowhides and rabbit-skins without damaging the collagen fibers. These properties confirm the feasibility of L9T protease in industrial applications, especially in leather processing.

The study of habitat characteristics and food sources of Luciola unmunsana - A Case Study of Sansungcheon, Jeonju City - (운문산반딧불이(Luciola unmunsana)의 서식지 특성과 먹이원에 관한 연구 - 전주시 산성천을 대상으로 -)

  • Lim, Hyun-Jeong;Kim, Jong-Man;Jeong, Moon-Sun
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.25 no.3
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    • pp.83-95
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    • 2022
  • This study aims to present primary data for habitat restoration and artificial breeding conditions of L. unmunsana by identifying the habitat conditions and the larvae's food sources. In order to investigate the habitat characteristics of the adult L. unmunsana and land snails, which are the primary food sources for the larvae, field surveys were conducted on a total of 10 habitats in south-central parts of Korea including Sanseongcheon, Jeonju. The results revealed that the L. unmunsana habitat in the Sanseongcheon area had a broadleaf forest with a multi-layered vegetation structure, adjacent water features, and the north/northeast/northwest slopes with little effect of artificial lighting. The adult L. unmunsana in the Sanseongcheon area appeared from the end of May to the end of June, and was especially intensively observed around the middle of June. The most active time was from 23:30 to 00:30 with a temperature range of 19~22℃ and higher than 80% humidity. The peak count of the observed adults L. unmunsana was a total of 774 on June 11, 2021. In the case of land snails, 11 families and 23 species were observed in 10 habitats of L. unmunsana, and Euphaedusa fusaniana was the most extensive and the most observed in the five survey areas. The land snails of L. unmunsana habitats are mostly found under the organic layers of leaves and a fallen tree branch in broadleaf forests, where a thick organic material layer buffers temperature changes and provides high humidity for various snails. These habitat conditions are suitable for the larva of L. unmunsana and land snails to inhabit, feed, hide and hibernate.

A Study on Characteristics of Underground Rappers' Fashion - Focusing on the Comparison Between China and Korea - (언더그라운드 래퍼 패션의 특성 - 한국과 중국의 비교를 중심으로 -)

  • Fan, Keyu;Ha, Jisoo
    • Fashion & Textile Research Journal
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    • v.24 no.5
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    • pp.493-504
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    • 2022
  • This study aims to gain a deeper understanding of the fashion styles of underground rappers in China and South Korea. Due to rappers' fashion influence on modern fashion trends, research on rapper fashion has been conducted steadily in the field of apparel. Qualitative research methodologies including literature research and in-depth interviews were the primary techniques used to solve the research questions. In-depth interviews were conducted with 10 Chinese underground rappers and 10 Korean underground rappers to reveal and explore their fashion style and view of fashion. As a result, the participants' fashion styles were categorized into four styles: authentic hip-hop fashion style, popularized hip-hop fashion style, easy sports casual style, and trendy street style. Rappers from both countries consider that their identity as rappers can be demonstrated through their hip-hop fashion style. The influence of hip-hop culture and the popularity of rap music differs between China and Korea. It affects not only the rapper's musical characteristics, but also their fashion style. While Korean underground rappers' fashions style is trendier, Chinese underground rappers' fashions style displays stronger characteristics of hip-hop fashion. Due to the public's negative view of rappers, some Korean underground rappers intentionally try to hide their identity by wearing a fashion style that differs from the authentic rapper image. Understanding the fashion styles of underground rappers in Korea and China is expected to assist in predicting future hip-hop culture and rapper fashion trends.

Malicious Code Injection Vulnerability Analysis in the Deflate Algorithm (Deflate 압축 알고리즘에서 악성코드 주입 취약점 분석)

  • Kim, Jung-hoon
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.32 no.5
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    • pp.869-879
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    • 2022
  • Through this study, we discovered that among three types of compressed data blocks generated through the Deflate algorithm, No-Payload Non-Compressed Block type (NPNCB) which has no literal data can be randomly generated and inserted between normal compressed blocks. In the header of the non-compressed block, there is a data area that exists only for byte alignment, and we called this area as DBA (Disposed Bit Area), where an attacker can hide various malicious codes and data. Finally we found the vulnerability that hides malicious codes or arbitrary data through inserting NPNCBs with infected DBA between normal compressed blocks according to a pre-designed attack scenario. Experiments show that even though contaminated NPNCB blocks were inserted between normal compressed blocks, commercial programs decoded normally contaminated zip file without any warning, and malicious code could be executed by the malicious decoder.

Spectrum of nitrous oxide intoxication related neurological disorders in Korea: a case series and literature review

  • Lee, Jungsoo;Park, Yangmi;Kim, Hyunkee;Kim, Nakhoon;Sung, Wonjae;Lee, Sanggon;Park, Jinseok
    • Annals of Clinical Neurophysiology
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    • v.23 no.2
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    • pp.108-116
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    • 2021
  • Background: Nitrous oxide (N2O) is used in surgery and dentistry for its anesthetic and analgesic effects. However, neurological and psychiatric manifestations of N2O abuse have been increasingly reported among Korean adults. The aim of this study was to demonstrate laboratory findings of N2O abuse in Korean patients. Methods: Patients diagnosed with N2O-induced neuropathy or myelopathy from August 2018 to December 2019 were enrolled. Their clinical presentations and laboratory and imaging findings were analyzed. Results: Sensory changes and limb weakness were present in nine of the enrolled patients. The laboratory findings revealed that seven patients had high homocysteine levels and five had high methylmalonic acid levels in their blood. Nerve conductions studies indicated that axonal neuropathy was present in four cases and longer F-wave and Hoffman's-reflex latencies were present in two cases. Signal changes in cervical spine imaging occurred in five patients, while two had normal results. Conclusions: Chronic N2O abuse can cause neurological damage or psychiatric problems. Because N2O is illegal for recreational use in Korea, patients tend to hide their history of use. Even though the spinal imaging results were normal, clinicians should consider the possibility of N2O use, and further electrophysiological tests should be applied for precise evaluations.