• Journal of the korean society of oceanography
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• 제39권1호
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• pp.57-71
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• 2004

Based on the data obtained under the China-Korea joint project (1997-2001) and historic observations, the distribution, transportation and sedimentation of sediment in the southern Yellow Sea (SYS) are discussed, and the controversial formation mechanism of muddy sediments is also explored. The sediment transport trend analysis indicates that the net transport direction of sediment in the central SYS (a fine-grained sediment deposited area) points to $123.4^{\circ}E,\;35.1^{\circ}N$, which is a possible sedimentation center in the central SYS. The sediment transport pattern is verified by the distribution of total suspended matter (TSM) concentration and ${\delta}^{13}C$ values of particulate organic carbon (POC), the latter indicates that the bottom water plays a more important role than the surface water in transporting the terrigenous material to the central deep-water area of the SYS, and the Yellow Sea circulation is an important control factor for the sediment transport pattern in the SYS. The carbon isotope signals of organic matter in sediments indicate that the Shandong subaqueous delta has high sedimentation rate and the deposited sediments originate mainly from the modern Yellow River. The terrigenous sediments in deep-water area of the SYS originate mainly from the old Yellow River and the modern Yellow River, and only a small portion originates from the modern Yangtze River. The analytical results of TSM and stable carbon isotopes are further confirmed by another independent tracer of sediment source, polycyclic aromatic hydrocarbons (PAHs). Five light mineral provinces in the SYS can be identified and they indicate inhomogeneity in sources and sedimentary environment. The modern shelf sedimentary processes in the SYS are controlled by shelf dynamic factors. The muddy depositional systems are produced in the shelf low-energy environments, which are controlled by some meso-scale cyclonic eddies (cold eddies) in the central SYS and the area southwest of the Cheju Island. On the contrary, an anticyclonic muddy depositional system (warm eddy sediment) appears in the southeast of the SYS (the area northwest of the Cheju Island). In this study, we give the cyclonic and anticyclonic eddy sedimentation patterns.

• 한국토양비료학회지
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• 제45권4호
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• pp.540-543
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• 2012

농업 부문에서 온실가스 배출량을 줄이기 위하여 녹비작물인 헤어리베치와 바이오숯을 이용하였을 때 밭작물 재배지에서 아산화질소 배출량 저감 효과를 평가하였다. 질소 시용량은 고추의 표준 시비량인 $190kg\;ha^{-1}$이었으며, 주 2회 시료를 채취하여 GC/ECD로 아산화질소를 분석하였다. 대조구인 질소질 비료(요소) 처리구의 아산화질소 배출량은 $1.14kg\;N_2O-N\;ha^{-1}$이었으며, 헤어리베치와 바이오숯+질소질 비료 처리구의 아산화질소 배출량은 각각 $0.61kg\;N_2O-N\;ha^{-1}$과 $0.86kg\;N_2O-N\;ha^{-1}$로 온실가스 배출을 각각 46.5%와 24.6% 줄였다.

• The Korean Journal of Physiology
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• 제17권2호
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• pp.119-124
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• 1983

The in vivo and in vitro buffer capacities of true plasma and tissue buffer capaciies were compared on dogs. Intracellular pH was determined on skeletal muscle by a modification of the method of Schloerb and Grantham using $C^{14}$ DMO. The in vivo curve for plasma or extracellular fluid has a much lower slope than the in vitro curve. The in vivo slope of skeletal muscle in the dog is approximately 20 sl. The slope for skeletal muscle in vivo falls between the in vitro and in vivo slopes of true plasma. It appears that intracellular hydrogen ion varies linearly with extracellular hydrogen ion when $CO_2$ tension is changed. Both hydrogen ion gradient and Hi/He ratio vary in skeletal muscle, with an increase in $CO_2$ tension. Infusion of 0.3N HCl gave two distinct patterns, the $H_i-H_e$ gradient decreased; and it would appear that very little hydrogen ion as such penetrated to the inside of the cells during the time of observation. Although lactic acid presumably enters the cell and the same of larger load was given as was used for hydrochloric acid, only very mild intracellular acidosis resulted, ostensibly due to metabolism of this substrate. Gluconic acid produced a more severe acidosis, both intracellularly and extracellularly, but with both of these acids the hydrogen ion gradient decreased and the $H_i/H_e$ ratio also decreased. The experiments on the dogs with hemorrhagic shock the hydrogen ion increase producing the acidosis originates inside the cells. Even so, the hydrogen ion gradient increased only very slightly in the acute experiments. This may suggest that even over short intervals of time skeletal muscle cells have a capacity to pump out hydrogen ions at a rate which maintains approximately the normal $H_i/H_e$ gradient when the source of the hydrogen ion is in the interior of the cell.

• 유기물자원화
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• 제23권2호
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• pp.28-35
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• 2015

본 연구에서는 학교 식당에서 배출되는 음식물류 폐기물을 준 회분식 액순환 건식 혐기성 소화를 이용해서 메탄가스를 생산하였다. 두 시스템이 운전되었는데, 각 시스템은 생물반응조와 액 저장조로 구성되었다. 각 생물반응조 바닥은 스크린이 설치되어 있어 2.5L의 분리된 액체는 액 저장조로 30분 동안 이송되고 이송이 끝나자마자 이송된 액은 반응조 상부로 투입된다. 이 같은 순환은 고농도의 VFAs를 가지는 액체를 반응조 상부로 공급하는 역할을 한다. 실험 초기에는 음식물류 폐기물/식종 미생물의 부피 비는 2:8이고 이는 9g VS/L의 유기물 부하로 나타낼 수 있다. 음식물류 폐기물 투입은 2주에 한번이었고, 평균 수분, 휘발물질, 회성분은 각각 65.91%, 32.73%과 1.36%로 파악되었다. 두 개의 고형물 부하가 연구되었는데, 각각 3.51g VS/d (System A)와 3.86g VS/d(System B)이다. 음식물류 폐기물 당 메탄 발생량은 각각 $6.30m^3CH_4/kgVS{\cdot}d$(System A)와 $4.94m^3CH_4/kgVS{\cdot}d$(System B)이다.

• Nutrition Research and Practice
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• 제5권5호
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• pp.396-403
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• 2011

Ceramides (Cer) comprise the major constituent of sphingolipids in the epidermis and are known to play diverse roles in the outermost layers of the skin including water retention and provision of a physical barrier. In addition, they can be hydrolyzed into free sphingoid bases such as $C_{18}$ sphingosine (SO) and $C_{18}$ sphinganine (SA) or can be further metabolized to $C_{18}$ So-1-phosphate (S1P) and $C_{18}$ Sa-1-phosphate (Sa1P) in keratinocytes. The significance of ceramide metabolites emerged from studies reporting altered levels of SO and SA in skin disorders and the role of S1P and Sa1P as signaling lipids. However, the overall metabolism of sphingoid bases and their phosphates during keratinocyte differentiation remains not fully understood. Therefore, in this study, we analyzed these Cer metabolites in the process of keratinocyte differentiation. Three distinct keratinocyte differentiation stages were prepared using 0.07 mM calcium (Ca$^{2+}$) (proliferation stage), 1.2 mM Ca$^{2+}$ (early differentiation stage) in serum-free medium, or serum-containing medium with vitamin C (50 ${\mu}L$/mL) (late differentiation stage). Serum-containing medium was also used to determine whether vitamin C increases the concentrations of sphingoid bases and their phosphates. The production of sphingoid bases and their phosphates after hydrolysis by alkaline phosphatase was determined using high-performance liquid chromatography. Compared to cells treated with 0.07 mM Ca$^{2+}$, levels of SO, SA, S1P, and SA1P were not altered after treatment with 1.2 mM Ca$^{2+}$. However, in keratinocytes cultured in serum-containing medium with vitamin C, levels of SO, SA, S1P, and SA1P were dramatically higher than those in 0.07- and l.2-mM Ca$^{2+}$-treated cells; however, compared to serum-containing medium alone, vitamin C did not significantly enhance their production. Taken together, we demonstrate that late differentiation induced by vitamin C and serum was accompanied by dramatic increases in the concentration of sphingoid bases and their phosphates, although vitamin C alone had no effect on their production.

• 대한환경공학회지
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• 제39권10호
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• pp.549-555
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• 2017

본 연구는 오존용해탱크 구조에 따라 마이크로오존버블의 분포, 에너지 소비, 수처리 효율이 어떻게 변하는지를 알아보고자 하였다. 오존용해탱크 내부의 격판은 압력의 변화, 전단력, 선회유동을 발생시키고 이는 버블 직경의 크기에 변화를 준다. 버블 직경의 크기는 내부의 격판에 따라 10.5%까지 차이가 났다. 오존 버블 직경의 변화는 에너지 소비와 관련이 깊다. 오존 버블이 작아질수록 버블생성에너지는 높아지지만 용존 효율이 올라가면서 오존생산에너지는 줄어들게 된다. 따라서 버블생성에너지와 오존생산에너지의 합이 최소인 마이크로오존버블을 생성하는 오존용해탱크를 선정하여야 한다. 동일한 양의 오존가스을 방류수에 주입하기 위해 소비된 에너지는 내부의 격판에 따라 2.5%까지 차이가 났다. 하지만 수처리 효율까지 고려한다면 오존용해탱크 선정 조건이 달라진다. 오존 버블이 작아질수록 증가하는 자유라디칼이 수처리에 매우 효율적이기 때문이다. 동일한 오존주입농도에서도 내부의 격판에 따라 수처리 효율이 10.4%까지 차이가 났다. 따라서 수처리 효율과 에너지 효율을 고려하여 합리적인 마이크로오존버블을 생성하는 오존용해탱크 구조에 대하여 연구하였다.

• 한국식품저장유통학회지
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• 제15권2호
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• pp.209-213
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• 2008

제빵용 시판 강력분에 미강을 첨가한 기능성 식빵 제조를 위하여 미강의 첨가량을 변화시키면서 반죽에 대한 물성, 식빵의 저장성, 제빵적성 및 관능검사를 조사하였다. 제빵의 비용적은 미강 5%, 10% 첨가구에서 각각 5.2%, 0.7%의 증가를 보였으나 미강 20%,30% 첨가구의 경우는 각각 대조구의 83%, 65%에 상당하는 비용적을 나타내어 감소하는 경향을 보였다. 미강을 첨가하여 제조한 식빵의 저장성 실험에서 구운 직후의 빵 crumb의 경도는 대조구에서 19.0으로 가장 부드러운 결과를 나타내었으나 저장 3일이 경과한 후에는 미강 5%첨가구가 가장 낮은 경도 값을 나타내었고 다음으로 대조구, 미강 10% 첨가구, 미강 20% 첨가구,미강 30% 첨가구의 순으로 경도 값이 증가하였다. 제빵에 있어서 반죽의 물성은 반죽의 작업성과 최종제품의 품질을 예상할 수 있는 척도로써 미강의 첨가가 증가할수록 반죽의 경도 값은 증가하는 경향을 보였고, 탄력성과 응집성은 반대로 미강의 첨가량이 증가할수록 감소하는 경향을 보였다. 반죽의 발효에 영향을 미치는 pH에 있어서 미강의 첨가에 의해 pH은 대조구 보다 감소하였으나 미강 첨가시료간의 유의적 차이는 나타나지 않았다. 미강의 첨가에 의해 변화하는 빵 내부의 색도를 측정한 결과에서는 L값은 미강 첨가에 의해 감소하는 경향을 나타내었으나 a값과 b값은 증가하는 경향을 나타내었다. 관능검사의 결과 전체적인 기호도에서는 대조구와 미강 5% 첨가구 시료간의 유의적 차이는 없었으나 외형과 향에서 미강 5% 첨가구 시료의 기호도가 높은 것으로 나타났다. 조직감, 관능적 특성의 결과를 종합해 볼 때 5에서 10% 정도의 미강 첨가 식빵이 영양이나 기능적인 면에서 우수한 것으로 사료된다.

• Nutrition Research and Practice
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• 제4권5호
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• pp.362-368
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• 2010

Oral administration of royal jelly (RJ) promotes wound healing in diabetic mice. Concerns have arisen regarding the efficacy of RJ on the wound healing process of normal skin cells. In this study, a wound was created by scratching normal human dermal fibroblasts, one of the major cells involved in the wound healing process. The area was promptly treated with RJ at varying concentrations of 0.1, 1.0, or 5 mg/ml for up to 48 hrs and migration was analyzed by evaluating closure of the wound margins. Furthermore, altered levels of lipids, which were recently reported to participate in the wound healing process, were analyzed by HPTLC and HPLC. Migration of fibroblasts peaked at 24 hrs after wounding. RJ treatment significantly accelerated the migration of fibroblasts in a dose-dependent manner at 8 hrs. Although RJ also accelerated the migration of fibroblasts at both 20 hrs and 24 hrs after wounding, the efficacy was less potent than at 8 hrs. Among various lipid classes within fibroblasts, the level of cholesterol was significantly decreased at 8 hrs following administration of both 0.1 ug/ml and 5 mg/ml RJ. Despite a dose-dependent increase in sphinganines, the levels of sphingosines, ceramides, and glucosylceramides were not altered with any concentration of RJ. We demonstrated that RJ enhances the migration of fibroblasts and alters the levels of various lipids involved in the wound healing process.

• Journal of Nutrition and Health
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• 제39권6호
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• pp.520-528
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• 2006

Free amino acids in epidermis function as a major component of Natural Moisturizing Factor (NMF), which maintains the optimal level of water in skin even at the low humidity. In fact, the depletion of free amino acids is reported in the epidermis of atopic dermatitis, the skin condition involving dryness. As an effort searching the dietary source for improving the level of water and free amino acid in epidermis, the dietary effects of silk protein, sericin (S) and fibroin (F) on trans epidermal water loss (TEWL), and plasma and epidermal levels of free amino acids were compared in this study. Thirty of male NC/Nga mice, an animal model of atopic dermatitis, were divided into three groups: group CA as an atopic control with control diet, group S: 1% sericin diet and group F: 1% fibroin diet. Ten of male BALB/c mice were served as group C (control group) with control diet. All mice were fed on diet and water ad libitum for 10weeks. Dry skin condition was established in group CA as TEWL was increased (148.7% of group C). In parallel, epidermal level of glutamate, one of major amino acids functioning as NMF, was dramatically decreased and epidermal levels of methionine and alanine were inversely elevated. Dietary supplementation of sericin (group S) reduced TEWL at the similar level with group C and increased epidermal levels of glutamate as well as serine and glycine, the other major amino acids as NMF. Despite a marked decrease of methionine and alanine, the reduction of TEWL and epidermal levels of glutamate, serine and glycine of group F were less than of group S. Furthermore, in contrast to similar levels of other free amino acids in plasma and epidermis of group S and group C, plasma and epidermal levels of other free amino acids, specifically phenylalanine, isoleucine, cysteine and tyrosine in epidermis of group F, were significantly higher than of group C. Together, our data demonstrate that dietary supplementation of sericin is more effective at improving dry skin condition that paralleled with the normalization of free amino acids in plasma and epidermis of NC/Nga mice.

• 한국산림과학회지
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• 제88권4호
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• pp.438-445
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• 1999

벌채수준 및 시료 채취시기에 따른 토양수내 이온농도 변화를 알아보기 위해 전라남도 곡성군에 위치한 성숙한 리기다소나무조림지를 대상으로 4개의 벌채처리구(무벌채구, $6m{\times}50m$, $10m{\times}50m$, $20m{\times}50m$ 대상개별처리구)를 설치하고 zero tension lysimeter를 이용하여 98년 6월 20일부터 8월 4일까지 6주동안 3차례 (7월 6일, 7월 30일, 8월 4일) 토양수를 채취하여 분석한 결과, 토양수내 이온농도는 채취 시기별로 뚜렷한 차이를 보였으나 벌채수준이나 채취 깊이별(10cm, 30cm)로는 차이가 나타나지 않았다. 조사기간동안 토양수내 이온농도의 크기는 $NO{_3}^-$ > $Ca^{2+}$ > $Mg^{2+}$ > $NH{_4}^+$ 순이었으며 $NO{_3}^-$의 평균 농도는 3.60mg/L, $Ca^{2+}$ 1.7mg/L, $Mg^{2+}$ 0.5mg/L, $NH{_4}^+$ 0.04mg/L 였다. 토양수내 이온농도는 $NH{_4}^+$를 제외하고는 토양수량과 이온농도 사이에 부의 상관관계(r= -0.31~-0.41)가 있었으며 리기다소나무조림지내 토양수 이온농도의 변화는 벌채처리보다는 강수유입 형태가 보다 더 큰 영향을 미치고 있는 것으로 나타났다.