• 대한본초학회지
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• 제24권4호
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• pp.39-47
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• 2009

Objectives : Hwangnyeonhaedok-tang (Huanglian Jiedu Tang; HHT) has been widely used for purging' 'fire' and lessening virulence of any pathogenic organism. However it has been rarely conducted to evaluate the immuno-biological activity. In this study, we evaluated anti-inflammatory effects of HHT in LPS-activated Raw264.7 cells. Methods : Cells were treated with $1\;{\mu}g/ml$ of LPS 1 h prior to the addition of HHT. Cell viability was measured by MTT assay. The production of NO was determined by reacting cultured medium with Griess reagent. PGE2 and proinflammatory cytokines were detected by ELISA. Expression of iNOS, COX-2, $I{\kappa}B{\alpha}$ and NF-${\kappa}B$ were analyzed by immunoblot analysis. Results : All three doses of HHT (0.03, 0.10 and 0.30 mg/ml) had no significant cytotoxicity during the entire experimental period. The levels of NO and PGE2 were dramatically augmented by LPS compared to control. However, HHT extract dose-dependently reduced these increases. Expression of iNOS and COX-2 protein were also decreased by treatment with HHT extract. Furthermore, HHT extract significantly reduced the nuclear translocation of NF-${\kappa}B$ which is critical in regulating inflammation through transcription of iNOS and COX-2. In addition, HHT extract reduced the elevated production of inflammatory cytokines including TNF-$\alpha$, IL-$1{\beta}$ and IL-6. Conclusions : The results in this study demonstrate that HHT extract exerts anti-inflammatory activities through the inhibition of NO, PGE2 and proinflammatory cytokines production via the suppression of NF-${\kappa}B$.

• 한국지진공학회:학술대회논문집
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• 한국지진공학회 2002년도 춘계 학술발표회 논문집
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• pp.159-168
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• 2002

본 연구에서는 최근에 개발된 Hilbert-Huang 변환(HHT) 기법의 교각시스템에서 손상위치추정을 위한 적용성을 분석하였다. HHT기법으로 시계열의 순간주파수를 분석할 수 있음을 이용하여, 손상에 기인한 비선형 거동이 발생하는 때에 순간주파수의 변화를 분석함으로서, 손상부재와 위치를 추정하는 방법이다. 손상을 입은 교각 시스템에 대하여 수치모의실험을 수행하였는데, 이 때에 주파수가 점차로 증가하는 입력하중을 사용하였다. 연구결과로부터, HHT기법이 한정된 갯수의 가속도센서를 이용하여 계측오차가 포함된 조건하에서도 교각에 발생한 손상위치를 적절히 색출할 수 있다는 것을 알 수 있다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XIII)
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• pp.521-524
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• 2003

An effective purification method was developed for producing Homoharringtonine (HHT), to guarantee high purity and yield from Cephalotaxus koreana. This process was a simple and efficient procedure, for the isolation and purification of HHT form the biomass of Cephalotaxus koreana, consisting of extract, adsorbent treatment, precipitation and followed by a chromatography. The extraction, adsorbent treatment and precipitation in pre-purification process allows for rapid and efficient separation of HHT from many compound and dramatically increases the yield and purity of crude HHT for HPLC purification steps compared to alternative processes. This purification processes serves to minimize solvent usage, size, and complexity of the operations for HHT purification.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2007년도 추계학술대회논문집
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• pp.895-902
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• 2007

Rotating machinery has two typical faults with clearance, one is partial rub and the other is looseness. Due to these faults, non-linear and non-stationary signals are occurred. Therefore, time-frequency analysis is necessary for exact fault diagnosis of rotating machinery. In this paper newly developed time-frequency analysis method, HHT(Hilbert-Huang Transform) is applied to fault diagnosis and compared with other method of FFT, SFFT and CWT. The results show that HHT can represent better resolution than any other method. Consequently, the faults of rotating machinery are diagnosed efficiently by using HHT.

• 대한기계학회논문집A
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• 제33권12호
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• pp.1442-1448
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• 2009

Transient characteristics of a signal can be effectively exhibited in time-frequency domain. Hilbert-Huang Transform (HHT) is one of the time-frequency domain analysis methods. HHT is known for its several advantages over other signal analysis methods. The capability of analyzing non-stationary or nonlinear characteristics of a signal is the primary advantage of HHT. Moreover, it is known that HHT can provide fine resolution in high frequency region and handle large size data efficiently. In this study, the effectiveness of Hilbert-Huang transform is illustrated by employing structural systems undergoing impact. A simple discrete system and an axially oscillating cantilever beam undertaking periodic impulsive force are chosen to show the effectiveness of HHT.

• 대한약침학회지
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• 제13권2호
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• pp.67-73
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• 2010

Objective : The purpose of this study is to determine the efficacy and safely of Hwangryunhaedok-tang Pharmacopuncture (HHT-PA) therapy on post-stroke depression (PSD) in stroke patients. Methods : Fifteen PSD patients has been recruited and evaluated with liver function test (LFT) and renal function lest (RFT) and depression scales, Beck Depress ion Inventory (BDI) and Hamilton Depression Rating Scale (HAM-D) before and after HHT-PA treatment. Results : HAM-D score significantly decreased after HHT-PA treatment regard less of improving of motor function. But BDI score did not decrease significantly. All results of LFT and RFT were in normal range before and after HHT-PA treatment Conclusion : These results showed that HHT-PA decrease PSD symptoms and has safety on stroke patients, although has no effect on improving of motor impairment.

• 한국전산구조공학회:학술대회논문집
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• 한국전산구조공학회 2002년도 가을 학술발표회 논문집
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• pp.453-458
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• 2002

A recently developed Hilbert-Huang transform (HHT) technique is applied to the detection of the damage locations of bridge structures. The HHT may be used to identify the locations of damages which exhibit nonlinear and non-stationary behavior, since the instantaneous frequency characteristics of the measured signal can be analyzed by the HHT. Numerical simulations were conducted on two bridge systems with damages using controlled excitations with sweeping frequency. Nonlinear plastic model using a gap element is employed to model the behavior of the cracked elements in the numerical simulations. The results indicate that the HHT method can reasonably identify the damage locations based on a limited number of acceleration sensors. Experimental study has been 실so carried out on a steel frame to confirm the applicability of the HHT to detect a structural connection with loosened bolts.

• 대한약침학회지
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• 제9권3호통권21호
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• pp.131-138
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• 2006

Objective : The aim of this study was to investigate the effect of HHT(Hwangryunhaedoktang Herbal-Acupuncture) on functional headache. Methods : 26 clinical experiment participants who experienced functional headache were gathered. The qualified patients were classified in two group, Normal saline injection group and Hwangryunhaedoktang Herbal-Acupuncture group. NS group was injected normal saline and HHT group was injected HHT at GB20, GB21, LI4. Treatment was applied 4 times every second day. The effects of both groups were analyzed using VAS scores and BPI(Brief Pain Inventory). Result : The VAS pain scores improved in both groups. the BPI scores decreased only in HHT group. The decrease rate of VAS scores and BPI scores was larger in HHT group. Conclusion : These results provided that HHT group is more effective than NS group. For clearly comparing the Effect of Hwangryunhaedoktang Herbal-Acupuncture on functional headache, it is need more sample's number and more treatment's duration.

• 대한한의학방제학회지
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• 제28권4호
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• pp.313-325
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• 2020

Objectives : To explore the associated potential pathways and molecular targets of Hwangryunhaedok-tang(HHT) by the approaches of network pharmacology and bioinformatics in traditional chinese medicine(TCM). Methods : Hwangryunhaedok-tang constituent drugs(Coptidis Rhizoma, CR; Scutellariae Radix, SR; Phellodendri Cortex, PC; Gardeniae Fructus, GF) and their processing types were searched from TCM systems pharmacology(TCMSP). The databases of TCMSP, Kyoto Encyclopedia of Genes and Genomes(KEGG), MCODE and STRING were used to gather information. The network of bioactive ingredients and target gene was constructed by Cytoscape software(version 3.8). Results : A total of 94 HHT active compounds(CR, 12; SR, 35; PC, 33; GF, 14, respectively) were found, and HHT were identified by TCMSP. Applications of KEGG and MCODE analysis indicates that total of 6 bioactive ingredients in the top 10% ranking were obtained and 32 diseases of HHT were screened. The molecular pathway analysis revealed that HHT exerts cancer, inflammation and cerebrovascular diseases effects by acting on several signaling pathway. In addition, HHT found that three genes(e.g. SPIN1, TRIM25, and APP) correlate with the aforementioned diseases. Conclusions : This study showed that network pharmacology analysis is useful to elucidate the complex mechanisms of action of HHT.

• Structural Engineering and Mechanics
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• 제39권1호
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• pp.1-20
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• 2011

This study investigates the feasibility of detecting structural damage using the HHT method. A damage detection index, the ratio of bandwidth (RB) is proposed. This index is highly correlated or approximately equal to the change of equivalent damping ratio for an intact structure incurring damage from strong ground motions. Based on an analysis of shaking table test data from benchmark models subjected to adjusted Kobe and El Centro earthquakes, the damage detection index is evaluated using the Hilbert-Huang Transform (HHT) and the Fast Fourier Transform (FFT) methods, respectively. Results indicate that, when the response of the structure is in the elastic region, the RB value only slightly changes in both the HHT and the FFT spectra. Additionally, RB values estimated from the HHT spectra vs. the PGA values change incrementally when the structure response is nonlinear i.e., member yielding occurs, but not in the RB curve from the FFT spectra. Moreover, the RB value of the top floor changes more than those from the other floors. Furthermore, structural damage is detected only when using the acceleration response data from the top floor. Therefore, the ratio of bandwidth RB estimated from the smoothed HHT spectra is an effective and sensitive damage index for detecting structural damage. Results of this study also demonstrate that the HHT is a powerful method in analyzing the nonlinear responses of steel structures to strong ground motions.