• 한국식품과학회지
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• 제44권2호
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• pp.196-201
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• 2012

본 연구에서는 청국장 및 볏짚에서 분리한 미생물중 decarboxylase 배지에서 탈탄산효소 활성이 낮은 균주로 $B.$ $subtilis$ HH12, $B.$ $subtilis$ HR254, $P.$ $barcinonensis$ KR97을 선발하였다. 이들 선발 미생물들의 histidine decarboxylase($hdc$) gene 과 tyrosine decarboxylase($tdc$) gene을 조사한 결과, $hdc$ gene은 HH12, HR254, KR97 균주에서는 검출되지 않았으나, $tdc$ gene은 HH12, HR254, KR110 균주에서 검출되었다. 또한 HPLC를 통해 선발 균주들의 배양상등액 중 amines 생성량을 분석한 결과, HH12, HR254, KR110 균주는 histamine이 검출되지 않았고 tyramine은 HH12, HR254, KR110 균주는 각각 6.09, 3.68, 6.30 mg/L로 검출되었다. 선발한 biogenic amines 저감화 균주들의 BAs 생성량을 decarboxylase 고생산 미생물로 사료되는 $B.$ $subtilis$ MC138 균주와 비교해보면 현저히 낮은 수준으로 나타났다.

• Pediatric Infection and Vaccine
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• 제22권3호
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• pp.186-193
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• 2015

목적: 소아병원 의료기관 종사자들의 손 위생 수행률을 계량화하고 분석함으로써 소아병원 종사자의 손 위생 수행실태에 대한 기본 자료를 구축하고자 수행되었다. 방법: 3차 의료기관이며 대학병원인 313병상 소아병원의 533명 직원을 대상으로 WHO 손 위생 모니터링 도구로 2010년도 11월 1일부터 12월 31일까지 직접관찰법으로 자료를 수집하였다. 결과: 총 관찰건수 2,999으로, 손 위생 수행률은 95.3%였고, 직종별로 간호사(97.7%), 의사(89.2%), 이송직(72.1%) (P<0.001), 부서별로 중환자실(92.5%), 외래(95.4%), 응급실(97.2%), 수술실(97.2%) 이었으며(P<0.001), 의사직급별로 전임의(97.5%), 교수(93.9%), 전공의(89.7%), 인턴(80.9%)이었다(P<0.001). 손 마찰(81.1%)을 많이 사용하였고, 손 씻기는 '환자 체액 노출 후'(37.7%), '환자 환경 접촉 후' (28.5%)에 많이 사용하였다. 손 위생 방법은 부서별로 차이가 없었으나(P=0.083), WHO 5 Moments 별로 차이가 있었다(P<0.001). 직종별 WHO 5 Moments 분포는 차이가 있었다(P<0.001). 손 위생 이행 odds ratio는 의사직 0.353 (95% CI, 0.241-0.519), 중환자실 0.291 (95% CI, 0.174-0.487), 외래 0.484 (95% CI, 0.281-0.834)이었다. 결론: 손 위생 수행은 직종별, 부서별로 차이가 있었다. 직종별, 부서별로 효과적인 손 위생 교육 및 훈련 프로그램 개발이 필요하다.

• 한국식품영양학회지
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• 제15권4호
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• pp.364-369
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• 2002

방선균은 토양 속에 다양하게 존재하는 미생물의 일종으로 그람 양성 진정세균으로 이차대사산물을 생산하는 시기와 포자 착생이 시작되는 세포분화의 시기가 밀접한 관련이 있다. S. griseus는 streptomycin을 비롯한 다양한 종류의 endopeptidase 및 exopeptidase들을 생산한다. 방선균에서의 protease 생산은 많은 경우에 이차대사산물이 형성되거나 형태분화가 유도되는 시기에 동시에 시작된다는 점에서 Pretense가 이차대사물질 생산 및 세포분화에 일정한 기능을 수행할 것이 라는 점을 시사하고 있다. 본 연구에서는 S. griseus IFO 13350에서 클로닝한 SGPD protease가 각 strain에서 형태학적으로나 생리적으로 어떠한 gene dosage 효과를 미치는지 조사하는 것이었다. sprD 유전자가 S.lividans를 숙주로 사용한 시스템에서 대량발현이 성공적으로 되는 것을 확인한 후, 본 유전자를 클로닝한 S. griseus IFO13350 균주와 이의 A-factor 결손주인 S. griseus HH1에 형질전환하였다. S. griseus HH1과 S. griseus IFO13350에서는 protease activity가 벡터만 도입된 대조군과 sprD 유전자가 들어간 형질전환체에서 큰 차이를 보이지 않았다. 또한 S. griseus IFO 13350 및 HH1 모두에서 생리학적·형태학적 분화의 차이를 발견하지 못하였다. Chymotrypsin계열의 pretense를 암호화하는 유전자만이 S. griseus에서 발현이 repression된다는 사실을 본 연구 결과를 통하여 알게 되었다. 이를 바탕으로 sprD유전자와 동일계열의 chymotrypsin 계열의 유전자들이 공통적으로 S. griseus에서 repression 되는 일반적인 기전이 있을 것으로 판단, chymotrypsin계열 유전자들의 promoter부분의 염기 상동성을 조사하였다 번역개시부위 바로 상부 유전자부터 상동성을 조사한 결과 적어도 상당부분의 염기배열이 잘 보존된 지역이 존재함을 알게 되었다. 향후 이들 발현기구의 조절기구를 연구함으로서 protease의 기능을 밝히는데 좋은 단서를 제공할 것으로 판단된다.

• 대한한방부인과학회지
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• 제27권3호
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• pp.1-11
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• 2014

Objectives: Hwangryunhaedok-tang (HH) is a representative heat clearing multiherb prescription. We evaluated the protective effect of HH against heat stress exposure in mice. Methods: Six weeks old ICR mice were used for this study. After $43^{\circ}C$ heat stress for 15 minutes, we evaluated the changes of motor activity and c-fos expression level to determine the proper heat stress and evaluation time. The subjects were divided into 4 groups (1. control group, 2. heat stress group with normal saline, 3. heat stress group with administration of 100 mg/kg, and 4. heat stress group with administration of 1000 mg/kg). After oral administration of HH once a day for 3 days, 2, 3, 4 groups were exposed to $43^{\circ}C$ heat stress for 15 minutes. Then, we evaluated the motor activity for 120 minutes and analyzed the c-fos expression using western blot. We investigated the effect of HH and its ingredients on c-fos expressions after heat stress. The mice were administrated HH, Scutellariae Radix, Coptidis Rhizoma, Phellodendri Cortex, and Gardeniae Fructus for 3 days. After one hour of last treatment, the mice were exposed to heat stress at $43^{\circ}C$ for 15 min. After two hours, the hypothalamus was dissociated and lysed to measure c-fos expression. Results: After oral administration of HH for 3 days, motor activity was recovered insignificantly. 100 mg/kg HH treatment reduced the c-fos expression after heat stress but insignificantly. Among the ingredients of HH, Coptidis Rhizoma and Scutellariae Radix treatment groups significantly reduced the c-fos after stress. Conclusions: These results show that Hwangryunhaedok-tang may be effective to reduce the heat stress response.

• Fisheries and Aquatic Sciences
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• 제20권3호
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• pp.3.1-3.8
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• 2017

Seahorse, a syngnathidae fish, is one of the important organisms used in Chinese traditional medicine. Hippocampus abdominalis, a seahorse species successfully cultured in Korea, was validated for use in food by the Ministry of Food and Drug Safety in February 2016; however. the validation was restricted to 50% of the entire composition. Therefore, to use H. abdominalis as a food ingredient, H. abdominalis has to be prepared as a mixture by adding other materials. In this study, the effect of H. abdominalis on muscles was investigated to scientifically verify its potential bioactivity. In addition, the anti-fatigue activity of a mixture comprising H. abdominalis and red ginseng (RG) was evaluated to commercially utilize H. abdominalis in food industry. H. abdominalis was hydrolyzed using Alcalase, a protease, and the effect of H. abdominalis hydrolysate (HH) on the muscles was assessed in C2C12 myoblasts by measuring cell proliferation and glycogen content. In addition, the mixtures comprising HH and RG were prepared at different percentages of RG to HH (20, 30, 40, 50, 60, 70, and 80% RG), and the anti-fatigue activity of these mixtures against oxidative stress was assessed in C2C12 myoblasts. In C2C12 myoblasts, $H_2O_2$-induced oxidative stress caused a decrease in viability and physical fatigue-related biomarkers such as glycogen and ATP contents. However, treatment with RG and HH mixtures increased cell viability and the content of fatigue-related biomarkers. In particular, the 80% RG mixture showed an optimum effect on cell viability and ATP synthesis activity. In this study, all results indicated that HH had anti-fatigue activity at concentrations approved for use in food by the law in Korea. Especially, an 80% RG to HH mixture can be used in food for ameliorating fatigue.

• Human Ecology Research
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• 제56권6호
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• pp.573-588
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• 2018

This study analyzed per capita consumption expenditure by the consumption quintile of Middle and Older Elderly Households and the variables that affected it. Sample of 2,983 households was collected from the 6th KLoSA in 2016. First, when comparing the HH's living expenses by the number of family members, two persons to a person ratio, more than six persons to a person ratio were 2.007 and 4.148, respectively. The ratio increased as the number of family member increased; however, the rate of ratio increase decreased. Second, the per capita living expenses for the first and fifth quintile were 5.11 million won and 16.93 million won at the per capita living expenses that applied the OECD's equivalence scale to the HH's living expenses. The per capita living expenses for the fifth quintile was 3.31 times higher than in the first quintile. Third, among the variables influencing per capita living expenses by the consumption quintile, the marital status was the significant for all consumption quintiles; however, HH's income, HH's total asset, HH's financial asset, pension, subjective perception of the economy class, home ownership, residence (metropolitan or small city), education (elementary school), participation groups were significant in some quintiles among all quintiles. Fourth, of the significant variables influencing per capita living expenses, the most influential variables were first quintile followed by second quintile, third quintile, fourth quintile, marital status, HH's income, home ownership, HH's total asset, participation groups, residence (metropolitan), subjective perception of the economy class, in order.

• Asian-Australasian Journal of Animal Sciences
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• 제22권1호
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• pp.7-12
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• 2009

Lmbr1 is regarded as a key gene that controls the digital model formation in early developmental stages of the chicken. However, there are few reports of lmbr1 expression levels and tendencies in 4-toe and 5-toe chicken species. Therefore, the objective of this study was to compare the lmbr1 expression in White Leghorn (4-toe) and Chinese Silky (5-toe). Firstly, total RNA was extracted from 14 different embryonic development stages (HH3 to HH31) in White Leghorn and Chinese Silky. Secondly, dramatic gene expression changes of lmbr1 were monitored by RT-PCR, which indicated a general up-down-up tendency with subtle differences between these two species. Moreover, Q-PCR reactions were performed to quantitate the expression level of lmbr1 in the 14 selected developmental stages. These data demonstrated a first lmbr1 expression peak of 18.68 and 15.32, a lmbr1 expression trough of 6.61 and 1.80, and a second lmbr1 expression peak of 22.33 and 12.48 in White Leghorn and Chinese Silky, respectively. Finally, embryonic in situ hybridization analysis identified that lmbr1 expressed in the ectoderm in HH21, HH23 and HH24 developmental stages in both species.

• 대한가정학회지
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• 제42권11호
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• pp.205-222
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• 2004

The purposes of this study were to examine urban household's monthly expenses for public pension and to analyze the contributing factors. Data for this study were from the 2002 Urban Household Survey and consisted of a sample of 21,093 urban households. Statistics used for the analysis were frequencies, means, ANOVA and multiple regression analysis. The major findings were as follows ; First, the average urban household monthly payment for the public pension was 104,036 won, consisting of 102,757 won for single earner households and 106,014 won for dual earner households. Second, the highest expenses for monthly public pension was urban households, followed by male household head(HH), HH's age from 41-50 years, HH's educational level was college, HH's job was public servant, family didn't live in Seoul, family w3s an extended family and family owned the house. Third, the significant factors affecting the urban household's monthly public pension were HH's gender, age, educational level, type of job, region, type of family, number of children, type of earner, monthly total income, increase of asset in a month and house ownership.

• 천문학논총
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• 제30권2호
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• pp.113-114
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• 2015

Recent high-resolution, high-sensitivity observations of protostellar jets have shown many to possess an underlying 'wiggle' structure. HH 211 is one such example where recent sub-mm observations revealed a clear reflection-symmetric wiggle. An explanation for this is that the HH211 jet source is moving as part of a protobinary system. Here we test this assumption by simulating HH211 through 3D hydrodynamic simulations using the pluto code with a molecular chemistry and cooling module, and initial conditions based on an analytical model derived from SMA observations. Molecular chemistry allows us to accurately plot synthetic molecular emission maps and position-velocity diagrams for direct comparison to observations, enabling us to test the observational assumptions and put constraints on the physical parameters of HH211. Our preliminary results show that the reflection-symmetric wiggle can be recreated through the assumption of a jet source being part of a binary system.

• 한국가금학회지
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• 제43권4호
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• pp.207-212
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• 2016

본 시험은 국내 실정에 맞는 토종 실용계 생산을 위한 13교배종의 능력을 조사하기 위하여 수행하였다. 공시동물은 국내에서 사육되고 있는 토종 순종계를 이용하여 생산된 종계 부계통 2종(A, B)과 모계종 6종(1, 2, 3, 4, 5, 6)을 교배하여 생산된 12종(1A, 1B, 2A, 2B, 3A, 3B, 4A, 4B, 5A, 5B, 6A, 6B)과 일반 토종닭 교배종('한협3호', HH)의 총 13종, 종당 100수씩 총 1,300수를 암수 합사하여 공시하였다. 시험사료는 0~7일령까지는 육계초이사료(CP 22.0% ME 3,150 kcal/kg), 8일령부터 체중 800 g까지는 육계전기사료(CP 19.0% ME 3,150 kcal/kg), 체중 800 g~12주령까지는 육계후기사료(CP 18.0% ME 3,150 kcal/kg)를 급여하였다. 주요 조사항목은 수정률과 부화율, 체중, 균일도, 사료요구율, 생산지수 그리고 경제성 등이었다. 수정률은 1B종이 가장 높았으며, 6B와 HH종이 낮게 나타났다. 수정란 대비 부화율은 87.5~89%, 입란대비 부화율은 76.9~92.3%로 나타났으며, 4B와 6B종이 낮은 수치를 보였다. HH종의 주령별 체중은 다른 교배종에 비해 낮게 나타났다. 체중의 CV가는 12.5~18.2%로 13종 모두 12%보다 높게 나타났으며, 특히 2B, 3B, 5B, HH종에서 높게 나타났다. 누적 사료요구율은 6, 8, 10, 12주령에 각각 1.7~2.3, 2.1~2.5, 2.4~2.7 및 2.7~3.2로 나타났다. 주령별 생산지수는 2A, 3A, HH종에서 낮게 나타났으며, 주령이 경과함에 따라 생산지수는 감소하였다. kg 증체당 사료비는 육계초기 1,069~1,255원, 육계전기 1,100~1,263원, 육계후기 1.468~1,696원으로 나타났다. 본 연구의 결과를 분석한 결과, 기존 토종닭보다 생산성이 우수하고 경제성을 갖춘 새로운 토종 실용계 선발이 가능하다는 결론을 도출하였다.