Browse > Article

Effect of the Overexpression of the sprD Gene Encoding Streptomyces griseus Pretense D for the Differentiation of Streptomyces griseus HH1  

이재학 (서일대학 식품영양과)
Publication Information
The Korean Journal of Food And Nutrition / v.15, no.4, 2002 , pp. 364-369 More about this Journal
Abstract
Streptomyces shows a eukaryotic characteristic that vegetative cell can grow into mycelial form and has morphological and physiological differentiation at a certain period during its life cycle. Streptomyces has been used for the production of many biologically active compounds, such as antibiotics and pronase. Production of second metabolites and differentiation of the vegetative cell share the certain period of its lift cycle. Therefore, second metabolites may affect the differentiation of the vegetative cell. One of the microbial hormone, called A-factor, regulates the production of second metabolites, sporulation and differentiation of the cells. Streptomyces griseus produces streptomycin as well as many different kinds of proteinase. As mentioned, period of proteinases production overlaps with the period of differentiation of the vegetative cells. Protease may play a important role for the differentiation of the cells. In this paper, function of the SGPD gene cloned from S. griseus IFO 13350 tested whether it affects for the differentiation of A-factor mutated S. griseus HH1 and S. griseus IFO13350. pWHM3 and pWHM3-sprD plasmid was transformed into S. griseus HH1 and S. griseus IFO13350. Chymotrypsin activity of the cultured medium of the transformants with pWHM3-sprD plasmid didn't show any change with that of the transformants with plasmid only. The transformants with pWHM3-sprD plasmid didn't show the increase of the production of actinorhodin as well as morphological change in S. griseus IFO 13350 and HH1, as well. The promoter sequences of the SGPA and SGPB gene which encode chymotrypsin-like protease, were compared with that of SGPD gene. Regulatory mechanism of gene expression of proteinase genes will be studied for the development of high production system for protease as well as the function of the proteases.
Keywords
sprD; SGPD; S. lividans; S. griseus IFO13350; S. griseus HH1;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Kim J.M.and Hong, S.K. : Streptomyces griseus HHI, and A-factor deficient mutant, produces diminished level of trypsin and increased level of metalloproteases. The Journal of Microbiology, 38(3), 160-168(2000)
2 Maniatis, T., Fritsch, E. F. and Sambrook, J. : Molecular cloning, Cold spring harbor laboratory (1982)
3 Hopwood, D. A., Bibb, M. J., Chater, K. F., Kieser, T., Bruton, C. J., Kieser, H. M., Lydiate, D. J., Smith, C. P. and Ward, J. M. : Genetic manipulation of Streptomyces : a laboratory manual, The John Innes Foundation, Norwich, England (1985)
4 Chi, W. J., Kim, J. M., Choi, S. S., Kang, D. K. and Hong, S. K. : Overproduction of SGPA and SGT Induces Morphological Changes in Streptomyces lividans. J. Microbiol. Biotechnol., 11(6), 1077-1086 (2001)
5 Trop, M. and Birk, Y. : The specificity of proteases from Streptomyces griseus (pronase). J. Biochem., 116, 19-25 (1970)   DOI
6 Olfason, R. W. and Smillie, L. B. : Enzymatic and physicochemical properties of Streptomyces griseus trypsin. Biochemistry, 14, 1161-1167 (1975)   DOI   ScienceOn
7 Awad, W. M. Jr., Brew, K., Powell, J. T., Russin, D. J., Seber, J. F., Siegel, S. and Vosbeck, K. D. : The proteolytic enzymes of the K-1 strain of Streptomyces griseus obtained from a commercial preparation (Pronase). ?. Evolutionary implications. pp. 77-91 (1976)
8 Moncheva, P.A., Danova, S. T., Antonova, S. K. and Jvanova. I. V. : Physiological role of extracellular proteases and calcium ions in the processes of differentiation and antibiotic production by Streptomyces albogriseolus 444. Antibiot. Khimioter., 42, 14-19 (1997)
9 Henderson, G., Krygsman, P., Liu, C. J., Davey, C. C. and Malek, L. T. : Characterization and structure of genes for proteases A and B from Streptomyces griseus. J. Bacteriol., 169, 3778-3784 (1987)   DOI
10 Horinouchi, S. and Beppu, T. : Regulation of secondary metabolism and morphogenesis in Strepmyces : A-factor as a microbial hormone and AfsR protein as a component of a two-component regrulatory system. Gene, 115, 167-172(1992)   DOI   PUBMED   ScienceOn
11 Hara, O. and beppu, T. : Induction of streptomycin inactivating enzyme by A-factor in Streptomyces griseus. J. Antibiot., 35,1208-1215(1982)   DOI
12 Kim, J. C., Cha, S. H., Jeong, S. T., Oh, S. K. and Byun, S. M. : Molecular cloning and nucleotide sequence of Streptomyces griseus trypsin gene. Biochem. Biophys. Res. Commun., 181, 707-713 (1991)   DOI   ScienceOn
13 Gregory, D. G., Ordaz, D. E. and Strohl, W. R. : Overexpression of extracellular protease activity by Streptomyces C5-A13 in fed-batch fermentation. Appl. Microbiol. Biotechnol., 31, 119-124 (1989)   DOI
14 Kim, I. S., Kim, H, T., Lee, H. S. and Lee, K. J. : Protase inhibitor production using Streptomyces sp. SMF13. J. Microbiol. Biotechnol., 1, 288-292 (1991)
15 Trop, M. and Birk, Y. : The trypsin-like enzyme from Streptomyces griseus (pronase). J. Biochem., 109, 475-476 (1968)   DOI
16 Choi, S. S., Chi, W. J., Lee, J. H., Kang, S. S., Jeong, B. C. and Hong, S. K. : Overexpression of the sprD Gene encoding Streptomyces griseus protease D stimulates actinorhodin production in Streptomyces lividans. The Journal of Microbiology, 39(4), 305-313 (2001)
17 Beppu, T. and Horinouchi, s. : Molecular mechanism of the A-Factor dependent control of secondary metabolism in streptomyces. Planta Medica, 57,S44-S47(1991)   DOI   PUBMED   ScienceOn
18 Hong S.K., Mastsumoto, A., Horinouchi, S. and Beppu,T. : Effects of protein kinase inhibitors on in vitro protein phosphrylation and cellular differentiation of Streptomyces griseus. Mol. Gen. Genet., 236,347-354(1993)   DOI   ScienceOn