• Title/Summary/Keyword: HEA

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Fabrication of Equiatomic CoCrFeMnNi High-Entropy Alloy by Metal Injection Molding Process Using Coarse-Sized Powders

  • Eun Seong Kim;Jae Man Park;Ji Sun Lee;Jungho Choe;Soung Yeoul Ahn;Sang Guk Jeong;Do Won Lee;Seong Jin Park;Hyoung Seop Kim
    • Journal of Powder Materials
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    • v.30 no.1
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    • pp.1-6
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    • 2023
  • High-entropy alloys (HEAs) are attracting attention because of their excellent properties and functions; however, they are relatively expensive compared with commercial alloys. Therefore, various efforts have been made to reduce the cost of raw materials. In this study, MIM is attempted using coarse equiatomic CoCrFeMnNi HEA powders. The mixing ratio (powder:binder) for HEA feedstock preparation is explored using torque rheometer. The block-shaped green parts are fabricated through a metal injection molding process using feedstock. The thermal debinding conditions are explored by thermogravimetric analysis, and solvent and thermal debinding are performed. It is densified under various sintering conditions considering the melting point of the HEA. The final product, which contains a small amount of non-FCC phase, is manufactured at a sintering temperature of 1250℃.

Characterization of MACS Isolated Cells from Differentiated Human ES Cells (인간 배아줄기세포로부터 분화된 세포에서 MACS 방법을 이용하여 분리한 세포의 특성에 대한 연구)

  • Cho, Jae Won;Lim, Chun Kyu;Shin, Mi Ra;Bang, Kyoung Hee;Koong, Mi Kyoung;Jun, Jin Hyun
    • Clinical and Experimental Reproductive Medicine
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    • v.33 no.3
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    • pp.171-178
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    • 2006
  • Objective: Human embryonic stem (ES) cells have a great potential in regenerative medicine and tissue engineering. The human ES cells could be differentiated into specific cell types by treatments of growth factors and alterations of gene expressions. However, the efficacy of guided differentiation and isolation of specific cells are still low. In this study, we characterized isolated cells from differentiated human ES cells by magnetic activated cell sorting (MACS) system using specific antibodies to cell surface markers. Methods: The undifferentiated hES cells (Miz-hESC4) were sub-cultured by mechanical isolation of colonies and embryoid bodies were spontaneously differentiated with DMEM containing 10% FBS for 2 weeks. The differentiated cells were isolated to positive and negative cells with MACS system using CD34, human epithelial antigen (HEA) and human fibroblast (HFB) antibodies, respectively. Observation of morphological changes and analysis of marker genes expression were performed during further culture of MACS isolated cells for 4 weeks. Results: Morphology of the CD34 positive cells was firstly round, and then it was changed to small polygonal shape after further culture. The HEA positive cells showed large polygonal, and the HFB positive spindle shape. In RT-PCR analysis of marker genes, the CD34 and HFB positive cells expressed endodermal and mesodermal genes, and HEA positive cells expressed ectodermal genes such as NESTIN and NF68KD. The marker genes expression pattern of CD34 positive cells changed during the extension of culture time. Conclusion: Our results showed the possibility of successful isolation of specific cells by MACS system from undirected differentiated human ES cells. Thus, MACS system and marker antibodies for specific cell types might be useful for guided differentiation and isolation of specific cells from human ES cells.

Comparison of Selective Media for Isolation and Detection of Shigella spp. from Foods (식품으로부터 쉬겔라 검출을 위한 분리배지 비교)

  • In, Ye-Won;Ha, Su-Jeong;Oh, Se-Wook
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.7
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    • pp.1025-1031
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    • 2011
  • The objective of this study was to compare the performances of conventional microbiological media used in isolation of Shigella spp. from foods. Total of six selective media, including MacConkey agar (MAC), Salmonella Shigella agar (SSA), desoxycholate citrate agar (DCA), xylose lysine desoxycholate agar (XLD), hektoen enteric agar (HEA), and CHROMagar, were tested. MAC showed almost the same colony numbers as compared to tryptic soy agar (TSA) while DCA showed significantly lower colony numbers when cultivated Shigella spp. was counted in each medium. In a food recovery test with beef, pork and shrimp, S. sonnei recovered well on CHROMagar (p<0.05). With lettuce and cabbage, S. sonnei displayed significantly significant recovery (p<0.05) on SSA in comparison with other selective media. Heat-injured cells recovered well on MAC and SSA. In a specificity test using Enterobacteriaceae strains, HEA was identified as having the highest specificity among the tested media. However, Morganella spp. could not be differentiated from Shigella spp. on any of the tested selective media. Shigella spp. precluded the possibility of isolation from foods by a single 'best' selective medium. Consequently, a combination of complementary selective media or selection of appropriate media according to cell conditions must be considered for comprehensive isolation.

dLoran Measurement in Yeongil Bay using the Pohang Loran-C (9930M) (포항 로란-C(9930M) 이용 영일만 dLoran 측정)

  • Lee, Chang Bok;Lee, Jong Koo;Lee, Young Kyu;Hwang, Sang-Wook;Lee, Sang Jeong;Yang, Sung-Hoon
    • Journal of Navigation and Port Research
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    • v.38 no.3
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    • pp.227-232
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    • 2014
  • There are three essential components of eLoran: dLoran, data map of ASF, and the Loran data channel. Particularly, dLoran improves navigation accuracy, which is the core technology of eLoran systems. The requirement of HEA's absolute accuracy, less than 20 meters, can be satisfied via dLoran measurements and their corrections. In this study, dLoran measurements using the Pohang Loran-C (9930M) station signal were conducted at Yeongil Bay. We established a dLoran reference station at Homigot Management Office for navigation aids within the Bay. We estimated the effectiveness of the dLoran between the reference site (Homigot Management Office) and a test site (Heunghwan beach) by measuring TOAs. We verified that the TOA data measured at these two regions were highly correlated. The temporal differences in the data between the dLoran reference station and test site were about 10~30 ns per day, which is equivalent to a ranging error of 3~9 m. This result shows that eLoran can meet the requirement of 8~20 meters position accuracy for maritime HEA by correcting the ASF at the user's receiver.