• Ocean and Polar Research
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• v.41 no.2
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• pp.79-88
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• 2019

In this study, the inhibitory effect of Atriplex gmelinii C. A. Mey. against the activity of MMP-2 and MMP-9 secreted from phorbol-12-myristate-13-acetate (PMA)-stimulated HT-1080 cells was evaluated by gelatin zymography and enzyme-linked immunosorbent assay (ELISA), reverse transcription polymerase-chain reaction (RT-PCR), and Western blot assay. Specimens of the halophyte A. gmelinii were extracted twice for 24 hr with methylene chloride ($CH_2Cl_2$), and then twice with methanol (MeOH), in turn. Each extract significantly inhibited the enzymatic activities in gelatin zymography and MMP ELISA kit, and expression of MMP-2 and 9 in mRNA and protein levels. Two crude extracts were combined and then the combined crude extracts were fractionated into n-hexane, 85% aqueous methanol (85% aq.MeOH), n-butanol (n-BuOH), and water ($H_2O$) fractions, according to solvent polarity. Among solvent-partitioned fractions, the 85% aq.MeOH fraction showed the strongest inhibitory effect against MMP-2 and -9 in gelatin zymography and MMP ELISA kit. In RT-PCR, all solvent-partitioned fractions significantly suppressed mRNA expression of MMP-2 and -9. On the other hand, in Western blot assay, all solvent-partitioned fractions except $H_2O$ significantly reduced expression levels of protein. HT 1080 cell migration was most significantly inhibited by the n-BuOH fraction followed by the 85% aq.MeOH and $H_2O$ fractions. These results suggest that A. gmelinii could be used as a potential source to inhibit tumor cell metastasis.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.54 no.5
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• pp.742-750
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• 2021

Ixeris repens is a type of halophyte that grows in high salinity sands found in coastal sand dunes and sandy shores. This study was conducted to investigate the contents, antioxidant potency, and physiological activities of I. repens. In analyses of general composition, carbohydrate, protein, ash, and moisture content were 57.42%, 10.48%, 11.99% and 10.29%, respectively. Potassium, calcium, sodium, and magnesium were its most prevalent minerals. The solvents used to extract I. repens were 70% ethanol, 80% methanol, and distilled water. Among the resultant extracts, ethanol and methanol extracts displayed higher total polyphenol and flavonoid contents than the water extract. ABTS (IC50, 0.12 mg/mL) and FRAP (0.77 mM) radical scavenging activity were highest in the water extract, while methanol extract exhibited the strongest DPPH radical scavenging activity (IC50, 1.32 mg/mL), NO scavenging activity (IC50, 4.10 mg/mL), and reducing power (EC50, 0.14 mg/mL). Tyrosinase, elastase, and α-glucosidase inhibitory activities were highest in the ethanol extract. The ethanol extract also possessed the most potent acetylcholinesterase inhibitory activity. These results indicate that I. repens may be useful as an antioxidant, and a functional substance in food and pharmaceutical materials.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.55 no.3
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• pp.310-318
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• 2022

Cnidium japonicum a biennial plant belonging to the family Umbelliferae, is a halophyte that grows in high-salinity areas of coastal sand dunes and sandy shores. This study was conducted to investigate the constituents, antioxidant activities, and physiological activities of C. japonicum. Mineral analyses revealed that potassium, sodium, calcium, and magnesium were the most prevalent minerals in C. japonicum. We used 80% ethanol, 80% methanol, and distilled water as solvents to prepare extracts from C. japonicum tissues, and the obtained extraction yields ranged between approximately 26% and 32%. Among the three extracts, the ethanol and methanol extracts had higher total polyphenol and flavonoid levels than the water extracts did. The antioxidant activities of methanol extracts were the highest among the various solvent extracts of C. japonicum as was the elastase/collagenase inhibitory activity. In contrast, the ethanol extract exhibited the highest tyrosinase inhibitory activity. Furthermore, the methanol extract possessed over 80% BACE1 (β-secretase) inhibitory activity at a final concentration of 20 ㎍/mL. Therefore, these results indicate that methanol and ethanol extracts of C. japonicum may be useful as antioxidant and functional substances in food and pharmaceutical material.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.54 no.6
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• pp.938-947
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• 2021

Messerschmidia sibirica is a halophyte that grows in high-salinity areas of coastal sand dunes and sandy shores. This study was conducted to investigate the constituents, antioxidant potency, and physiological activities of M. sibirica. Mineral analyses revealed that potassium, calcium, sodium, and magnesium were the most prevalent minerals in M. sibirica leaves and stems. We used 70% ethanol, 80% methanol, and distilled water as solvents to prepare extracts from M. sibirica tissues, with extraction yields of between approximately 19% and 27% being obtained. Among the six types of extract, the leaf ethanol extract (LEE) was characterized by the highest total polyphenol and flavonoid contents, and the antioxidant activities of the LEE were also the highest among the different solvent extracts. In addition, the leaf water extract was shown to have the highest tyrosinase and α-glucosidase inhibitory activities, whereas the leaf methanol extract was found to have the highest elastase inhibitory activity. Notably, all leaf extracts were established to have more than 75% β-secretase (BACE1) inhibitory activity at a final concentration of 0.5 mg/mL. These results indicate that leaf extracts of M. sibirica may have beneficial antioxidant properties, and could thus have potential application as functional supplements in food and pharmaceutical products.

• Ocean and Polar Research
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• v.44 no.4
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• pp.311-317
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• 2022

Seed germination and seedling growth of a halophyte, Suaeda japonica were examined to measure the effects of seed preservation periods and various soil compositions in the mudflat of Saemangeum, Korea. Seed germination declined from 74.30% to 44.30% as the preservation period increased from 4 months to 12 months. After 14 days of seeding, seed germination was lowest in sandy soil (SS) at 8.67% and highest in earthworm cast+leaf mold (EC+LM) at 67.33%, showing 4~5 times higher than SS. The average lengths of seedlings were 12.20 mm in SS and 42.20 mm in EC+LM, respectively. These results suggest that seed germination and seedling growth enhanced in the soil that has more organic matter. In the experiment mixing Saemangeum soil (SMS), seed germination was from 4.00% in SMS to 88.00% in the soil of SMS+EC+LM after 14 days. Similar growth patterns were observed in the length of seedlings from 5.00 mm in SMS to 49.60 mm in SMS+EC+LM. The present results indicate that a population of Suaeda japonica could be established by stimulating seed germination and seedling growth by the addition of organic matters such as EC, LM, and EC+LM in the Saemangeum mud flat.

• Ocean and Polar Research
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• v.44 no.2
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• pp.127-137
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• 2022

This study examined the growth pattern and environmental factors affecting the growth of the halophyte, Suaeda japonica, which is prevalent on tidal flats in the west coast of Korea in order to calculate annual carbon production. Quantitative sampling was conducted every month for three years from 2018 to 2020 on salt marshes located on the southern coast of Ganghwa Island. In terms of annual density affected by the germination rate at first period, especially when air temperature for winter time was constantly below 0℃ for long periods of time, germination decreased and precipitation in summer also exerted an influence. In terms of annual growth with regard to length, the part below the ground grew rapidly in the beginning after budding, while the part above ground grew at a relatively steady rate at all times. With regard to biomass, the part below the ground also increased from April in a manner similar to length growth, but decreased drastically from September with leaves falling off and water loss occurring. The part above ground showed a rapid increase from the beginning of the rainy season. Size-frequency distribution revealed broader patterns after the rainy season as individual growth varied, but from September, it stopped at all year. High growth rates were recorded in the initial phase of growth after budding and growth was rapid, but growth declined in summer when biomass increased. The annual mean production based on growth rate was calculated at 352 gDWt/m²/yr, and the highest production was 519 gDWt/m²/yr in 2018, but it has decreased since 2019. Annual carbon production was at calculated 143.41 gC/m²/yr for Suaeda japonica in the vicinity of the southern coast of Ganghwa Island.

• Korean Journal of Pharmacognosy
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• v.48 no.1
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• pp.1-4
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• 2017

Four polyketides, tyrosol (1), (3R,4R)-(-)-4-hydroxymellein (2), (3R,4S)-(-)-4-hydroxymellein (3), and 1-phenyl-1,2-ethanediol (4) were isolated from organic extracts of cultures of an endophytic fungus Arthrinium sp. (JS420) isolated from stem of a halophyte Suaeda japonica Makico. Chemical structures of the isolated compounds were elucidated by comparison of their spectral data such as NMR and ESIMS with reported literature values. Among the isolated compounds, 3 and 4 were isolated for the first time from this fungus.

• Ocean and Polar Research
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• v.24 no.1
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• pp.79-86
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• 2002

It was found that 14 coastal habitats in South Korea have comparetively natural vegetation. The habitats were classified into three types - intertidal flats, sand dunes, and estuaries. There were four intertidal flats, five sand dunes and five estuaries. Except for Cynodon dactylon and Tetragonia tetragonoides, all of the main halophytes and sand dune plants were found in the habitats. These two species were mainly distributed on the southern coast. This study identified coastal vegetation, such as pure stands of Suaeda japonica on intertidal flats, mixed halophyte communities around the high-water mark, pure stands of Vitex rotundifolia on stable sand dunes, mixed communities dominated by Carex kobomugi on unstable sand dunes, and pure stands of Phragmites communis in estuaries. The types of coastal vegetation may depend on sediment types, the inundation time of seawater and the stability of sediments.

• Journal of Plant Biology
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• v.13 no.1
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• pp.25-32
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• 1970

Ecological study on the reclaimed high saline soil was carried out throughout a year(1969) to elucidate the changes of the structure of halophytes communiteis and the possibilities of desalination from high saline soil by absorption of chloride ion. Results from this studies are summarized as followings; 1) The growth rates of halophytes showed a variation; maximum growth rate of Salicornia appeared on August, Chenopodium on July, Suaeda on July, Aster on August and Scirpus on June. 2) Changes of frequency of each halophyte were varied in accordance with species. Chenopodium and Salicornia have the highest frequency of all on May. However, frequency as well as density of halophytes decreased after on May due to competition for absorbing moisture in plant communities. 3) The terrestrial plants which were succeed into the reclaimed tidal land had herborized 25 species on the both side of irrigation route. 4) Each of the maximum chloride uptake by halophytes appeared on May(Salicornia and Aster), on June(Chenopodium and Scirpus), and on August(Suaeda), respectirecely 5) Among the halophytes, Salicornia was confirmed to absorb the highest amount of chloride. A possible amount of chloride uptake by all halophytes per 100 square cm reached about 24,629. ppm.

• Mycobiology
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• v.48 no.2
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• pp.133-138
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• 2020

Talaromyces halophytorum sp. nov. was isolated from the roots of halophyte Limonium tetragonum collected from Seocheon-gun, Korea in November 2015. It showed a slow growth on yeast extract sucrose agar at 25 ℃, no growth at 4 ℃ or 37 ℃ and produced smooth-walled and globose to sub-globose conidia. T. halophytorum is phylogenetically distinct from the other reported Talaromyces species of section Trachyspermi based on multi-locus sequence typing results using partial fragments of β-tubulin, calmodulin, ITS, and RNA polymerase II genes.