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http://dx.doi.org/10.4217/OPR.2019.41.2.079

Inhibition of MMP-2 and -9 by Crude Extracts and Their Solvent-partitioned Fractions from the Halophyte Atriplex gmelinii  

Park, Min Jeong (Division of Marine Bioscience, Korea Maritime & Ocean University)
Kim, Junse (Ocean Science & Technology School, Korea Maritime & Ocean University)
Kong, Chang-Suk (Department of Food and Nutrition, College of Medical and Life Sciences Silla University)
Seo, Youngwan (Division of Marine Bioscience, Korea Maritime & Ocean University)
Publication Information
Ocean and Polar Research / v.41, no.2, 2019 , pp. 79-88 More about this Journal
Abstract
In this study, the inhibitory effect of Atriplex gmelinii C. A. Mey. against the activity of MMP-2 and MMP-9 secreted from phorbol-12-myristate-13-acetate (PMA)-stimulated HT-1080 cells was evaluated by gelatin zymography and enzyme-linked immunosorbent assay (ELISA), reverse transcription polymerase-chain reaction (RT-PCR), and Western blot assay. Specimens of the halophyte A. gmelinii were extracted twice for 24 hr with methylene chloride ($CH_2Cl_2$), and then twice with methanol (MeOH), in turn. Each extract significantly inhibited the enzymatic activities in gelatin zymography and MMP ELISA kit, and expression of MMP-2 and 9 in mRNA and protein levels. Two crude extracts were combined and then the combined crude extracts were fractionated into n-hexane, 85% aqueous methanol (85% aq.MeOH), n-butanol (n-BuOH), and water ($H_2O$) fractions, according to solvent polarity. Among solvent-partitioned fractions, the 85% aq.MeOH fraction showed the strongest inhibitory effect against MMP-2 and -9 in gelatin zymography and MMP ELISA kit. In RT-PCR, all solvent-partitioned fractions significantly suppressed mRNA expression of MMP-2 and -9. On the other hand, in Western blot assay, all solvent-partitioned fractions except $H_2O$ significantly reduced expression levels of protein. HT 1080 cell migration was most significantly inhibited by the n-BuOH fraction followed by the 85% aq.MeOH and $H_2O$ fractions. These results suggest that A. gmelinii could be used as a potential source to inhibit tumor cell metastasis.
Keywords
Atriplex gmelinii; HT-1080; MMP-2; MMP-9;
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Times Cited By KSCI : 4  (Citation Analysis)
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