• Title/Summary/Keyword: HAG-100

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Assessment of Nucleus-DNA Damage in Red Pepper Cells Treated with γ-Radiation through Comet Assay (Comet 분석을 통한 방사선처리 고추세포의 핵 DNA 손상평가)

  • An, Jung-Hee;Back, Myung-Hwa;Kim, Jae-Sung;Jeong, Jeong-Hag;Kwon, Soon-Tae
    • Journal of Plant Biotechnology
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    • v.31 no.3
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    • pp.225-230
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    • 2004
  • We employed single cell gel electrophoresis method (comet assay) to analyze the degree of nucleus-DNA damage in the leaves of red pepper (Capsicum annuum L.) seedlings exposed to $^{60}$ CO v-radiation stress. Nucleus-DNA damage was measured as the ratio of tail length (T) to head length (H) in individual comet image isolated from pepper leaf cell. The T/H ratio of control-cells and treated-cells at 50 or 100 Gy were 1.28 and 3.54 or 3.39, respectively, suggesting that nuclei of pepper cells were severely damaged in the integrity of DNA strand by the treatment of enhanced v-radiation. The percentage of head-DNA in control-cells was 76.8%, whereas those of 50 and 100 Gy treated-cells were 55.9% and 59.9%, respectively. Pretreatment of low dose (4 to 20 Gy) radiation to seeds decreased DNA-damage in the leaves of seedlings treated with high dose radiation at 50 or 100 Gy. In this experiment, we developed a sensitive, reliable and rapid method for evaluating genotoxic effect in the nuclei of plant cells by employing comet assay.

Relationship between the HVM and Cut-Off Level of Means-End Chain (수단-목적사슬(Means-End)이론의 컷오프(Cut-off) 수준과 가치 맵(Hierarchical Value Map)의 관계 분석)

  • Han, Hag-Chin;Cho, Moon-Sik;Oh, Ju-Seong;Seo, Jung-Mo
    • The Journal of the Korea Contents Association
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    • v.11 no.4
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    • pp.414-427
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    • 2011
  • The Means-end theory has successfully been applied in academics circles and is one of the qualitative methodologies, aiming at classifying into groups based on the specific values that they pursue. The more specific purposes of this study is to identify the relationship between the levels of abstraction and HVM(Hierarchical Value Map) and to identify the statistical changes in frequency activated cell and ladders among A-C-V. And lastly, to suggest the HVM according to the levels of abstraction. The subjects of this study includes the 100 hikers who joined the hiking at least once a year for 2007. The results of analysis show that as the level of abstraction goes up, the HVM itself is depicted more simple and the loss of information is also occurred. Consequently, the decision of levels of abstraction is should be made on the basis of objective validity.

Fatty Acid Profle of Egg in Laying Hens with Different Rearing System: A Field Study (사육방식에 따른 계란의 지방산 조성: 현장연구를 중심으로)

  • Choi, In-Hag
    • Journal of Environmental Science International
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    • v.22 no.6
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    • pp.773-777
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    • 2013
  • This study examined the effects of cage rearing system and backyard raising system on fatty acid profile of egg in laying hens during 9 weeks. Two hundred hens, 70-wk-old, Hy-line Brown, were used in this study and fed basal diets. 100 hens, randomly collected from facility, were transferred to cage rearing system and 100 remaining hens were moved to backyard raising system. For overall period, higher unsaturated fatty acid percentages in two different rearing systems than did saturated fatty acid (SFA) were observed. In unsaturated fatty acid compositions, both rearing system showed a higher mono unsaturated fatty acid (MUFA) contents than poly unsaturated fatty acid (PUFA) contents. Egg quality traits of laying hens reared in backyard system had considerably lower contents of SFA and higher contents of MUFA compared with eggs from cage rearing system (P<0.05). In addition, eggs from cage rearing system resulted in a significantly lower n-3 contents and higher n-6:n-3 ratios and CLA contents in comparison with backyard raising system (P<0.05). However, no significant effects of two different rearing systems on PUFA, PUFA:SFA ratio, and n-6 contents were observed during the experimental period. In conclusion, this result suggests that cage rearing system and backyard raising system will play an important role in improving egg production as functional foods.

Utilization of Chemical Blends to Increase Nitrogen and Decrease Pathogens in Duck Litter

  • Choi, In-Hag
    • Journal of Environmental Science International
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    • v.26 no.10
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    • pp.1181-1184
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    • 2017
  • This study examined the effects of chemical blends (a combination of alum and aluminum chloride) on pH, N, and pathogens in duck litter during a six-week experiment. In total, 240 Pekin ducks (160 males and 80 females) were individually distributed into 16 pens, in a randomized experimental design consisting of four treatments and four replicate pens per treatment. Our treatments included a control, T1 (75 g alum + 75 g aluminum chloride/kg duck litter), T2 (100 g alum + 100 g aluminum chloride/kg duck litter), and T3 (150 g alum + 150 g aluminum chloride/kg duck litter). There was no difference among treatments in pH and Total N (TN) at weeks 2, 4, and 6 and weeks 1, 4, 5, and 6, respectively. However, there were significant differences in both pH and TN among treatments at weeks 1, 3, and 5 and weeks 2 and 3, respectively. Regarding pathogens, we found small differences in all treatments in Escherichia coli populations from weeks 1 to 5 and in Salmonella enterica populations from weeks 1 to 3. In conclusion, the addition of chemical blends to duck litter increased TN, which resulted in a lower litter pH, but did not significantly affect pathogen populations.

Loss Comparison of Half and Full Bridge Converter according to Switching Methods (하프 및 풀 브리지 컨버터의 스위칭 방법에 따른 손실비교)

  • Ko, Eu-Sock;Lee, Dong-Hyun;Lee, Sang-Ri;Kim, Hag-Wone;Cho, Kwan-Yuhl
    • Proceedings of the KIPE Conference
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    • 2013.11a
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    • pp.99-100
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    • 2013
  • 기존 용접 컨버터는 풀 브릿지 컨버터는 하드스위칭을 하게 되는데, 이는 스위칭 소자로 사용되는 IGBT의 턴 오프 손실을 증가시키는 단점을 가지고 있다. 본 논문에서는 스위치의 손실을 줄이고자 기존의 풀 브릿지 컨버터에 ZVS(zero voltage switching) 턴 오프 방식를 적용한 회로와 하드 스위칭 방법에 대하여 손실을 PSIM 모의해석을 통하여 비교 분석하였다.

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Estimation of Bioconcentration Factors in Fish for Organic Nonelectrolytes Using the Linear Solvation Energy Relationship

  • Jung Hag Park;Eun Hee Cho
    • Bulletin of the Korean Chemical Society
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    • v.14 no.4
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    • pp.457-461
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    • 1993
  • Bioconcentration factors (BCF) in fish of organic nonelectrolytes are well correlated by a linear solvation energy relationship (LSER) of the form : log BCF= -0.95 + 4.74 $V_I/100 - 4.39{\beta} + 0.88{\alpha}$ where $V_I$ is the intrinsic solute molecular volume and ${\beta}$ and ${\alpha}$ are the solvatochromic parameters that measure hydrogen bond acceptor basicity and donor acidity of the compound. The LSER model can not only correlate the property with an accuracy comparable to molecular connectivity model but also provide a quantitative informationon on the nature and relative strength of solute-target system interactions affecting the property of interest. Such an information can hardly be obtained from molecular connectivity model.

Change of Rooting Potential as Affected by Rooting Promoter Treatment in Cuttings of Corylopsis coreana (히어리(Corylopsis coreana)의 삽목번식시 발근촉진제의 처리에 따른 발근력 변화)

  • Kwon, Soon-Tae;Kim, Jae-Chang;Jeong, Jeong-Hag
    • FLOWER RESEARCH JOURNAL
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    • v.19 no.4
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    • pp.202-205
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    • 2011
  • This study was conducted to determine the effects of several rooting promoter treatments on rooting of Corylopsis coreana cutttings for mass production. In first year experiment, no effect of rooting promoters was observed in hardwood cuttings taken on March 20. However, In softwood cuttings of June 20, rooting potential was greatly increased by IBA $100mg{\cdot}L^{-1}$ dipping treatment for 24 hours. Particularly, IBA $100mg{\cdot}L^{-1}$ 24 hour dipping treatment greatly shortened the days required for the rooting. In second year experiment, the result of first year was reconfirmed. Almost of all the cuttings (97.8% of rooting rate) were well rooted after only 30 days after cutting by IBA $200mg{\cdot}L^{-1}$ 24 hour dipping treatment. In this treatment, numbers and length of root were also prominent compared with any other treatment results.

Antithrombotic Efficacy of Protaetia brevitarsis Extract (흰점박이꽃무지(Protaetia brevitarsis) 추출물의 항혈전 효능)

  • Choi, In-Hag;Yu, Ri;Lim, Yeon-Ji;Choi, Gyu-Sung;Choi, Sung-Up;Hwang, Jong-Ik;Son, Jin-Sung;Chung, Tae-Ho
    • Journal of Environmental Science International
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    • v.28 no.7
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    • pp.639-643
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    • 2019
  • We aimed to evaluate antithrombotic efficacy of Protaetia brevitarsis extract during 21 days. Rats (SPF rat, weight 240~260 g) were divided into 16 groups (5 rats per group), they were: control group and Protaetia brevitarsis extract groups with dose of 0.1, 0.5, 1, 2.5, 5, 10, 25, 50, 75, 100, 200, 250, 500, 750, 1,000 mg/kg kg of body weight. Thromboplastin time (PT) and activated partial thromboplastin time (aPPT) as antithrombotic efficacy were tested in this animal experiment (at 7, 14 and 21 days). Overall, the admistration dose of Protaetia brevitarsis extract over 50 mg/kg at 7, 14 and 21 days for PT and over 25 mg/kg at 7, 14 and 21 days for aPPT tented to be longer than that of other groups. In addition, the optimal admistration doses of Protaetia brevitarsis extract to improves antithrombotic efficacy were 75, 100, 200 and 250 mg/kg at 7, 14 and 21 days for PT (p<0.05) and 50 and 100 mg/kg at 7 days, 75 mg/kg at 14 days, or 50, 100, 200 and 250 mg/kg at 21 days for aPPT (p<0.05). It can be concluded that Protaetia brevitarsis extract at optimal levels have antithrombotic efficacy.

Effects of Liquid Aluminum Chloride Amendments to Rice Hulls on pH and Soluble Reactive Phosphorus Contents (액상 염화알루미늄을 왕겨에 첨가시 pH와 수용성 인 함량에 미치는 영향)

  • Choi, In-Hag;Yi, Seong-Joon;Choi, Jung-Hoon
    • Journal of Environmental Science International
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    • v.18 no.5
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    • pp.589-594
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    • 2009
  • The goals of this study were conducted to investigate the effects of applying liquid aluminum chloride ($AlCl_3$) to rice hulls on pH and soluble reactive P (SRP). A total of 800 broiler chicks (4 treatments $\times$ 4 replicates $\times$ 50 birds) were housed into 16 floor pens in a single house for 5 weeks. The treatments were divided into 4 groups: control, 100 g of liquid $AlCl_3/kg$ of rice hulls, 200 g of liquid $AlCl_3/kg$ of rice hulls, and 300 g of liquid $AlCl_3/kg$ of rice hulls. Liquid $AlCl_3$ was sprayed on the rice hulls surface at a rate of 100 g, 200 g, and 300 g liquid $AlCl_3$ per kg rice hull. pH values and SRP contents were significantly decreased (P<0.05) with the increased liquid $AlCl_3$ levels in comparison with control. However, no significant differences in SRP contents were observed among all treatments at 3 and 4 weeks. Applying 100 g, 200 g, and 300 g liquid $AlCl_3$ to rice hulls reduced SRP contents by 18, 25, and 52% for 5 weeks, respectively, compared with the controls. In conclusion, these results suggest that using liquid $AlCl_3$ on rice hulls should be promising for reducing water contamination and resulted in a reduction in SRP contents, which reduced pH.

Evaluation of Mixed Korean Red Ginseng Marc with Aluminum Sulfate on Gas Concentration and VFA in Poultry Litter in Comparison with Aluminum Sulfate: In Terms of Livestock and Environment Managements (깔짚에서 발생되는 가스와 휘발성지방산에 대한 황산알루미늄과 비교 시 혼합 홍삼박제제의 평가: 축산환경 경영관점에서)

  • Choi, In-Hag
    • Journal of Environmental Science International
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    • v.24 no.4
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    • pp.549-554
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    • 2015
  • This study was conducted to determine the effects of mixed Korean red ginseng marc with aluminum sulfate on gas concentration and volatile fatty acid (VFA) in poultry litter during 4 weeks in terms of livestock and environment managements. A total of 240 broiler chicks were randomly allocated to four treatments in four replications and 15 birds per replicate. The four treatments was mixed to rice hull under each pen at 0, 10 g or 20 g red ginseng marc + 90g aluminum sulfate, and 100g aluminum sulfate per kg poultry litter (rice hulls). Carbon dioxide, methane, acetic acid, and propionic acids were measured weekly. The results that could be available include: First, during the experimental period, carbon dioxide emissions were not remarkably different among treatments. Second, no differences were observed among treatments in methane emissions at 2 weeks through 4 weeks, but at 1 week, the reduction in methane emissions was in following order: 100 g aluminum sulfate > 20 g red ginseng marc + 90 g aluminum sulfate > 10 g red ginseng marc + 90 g aluminum sulfate > control. Third, in spite of statistically differences, treatment with 10 g or 20 g red ginseng marc + 90g aluminum sulfate, and 100g aluminum sulfate reduced acetic acid and propionic acid as a function of time, except acetic acid in aluminum sulfate treatment at 2 and 4 weeks. In conclusion, the results indicated that like aluminum sulfate, using 10 g or 20 g red ginseng marc with aluminum sulfate was effective in decreasing methane and propionic acid released from poultry litter.