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Study on the Salt Tolerance of Rice and Other Crops in Reclaimed Soil Areas. -6. On the Effects of Increased N. P. K. Applications for Rice Plant in Reclaimed Salty Areas (간척지(干拓地)에서 수도(水稻) 및 기타작물(其他作物)의 내염성(耐鹽性)에 관(關)한 연구(硏究) -6. 염분간척지(鹽分干拓地)에서 수도(水稻)에 대한 N, P, K,의 증비효과에 관(關)하여)

  • Im, H.B.
    • Korean Journal of Soil Science and Fertilizer
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    • v.3 no.1
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    • pp.35-41
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    • 1970
  • The experiment was conducted at the salt concentration of 0.5% and 1% end of April, respectively, in low and high-salty and the non-salty areas of silt loam with the Nongkwang, rice variety. The factorial design with confounding blocks of 3 levels each of 10, 15 and 20 kg of N, 8, 12 and 16kg of phosphate and potash, respectively, per 10a was applied. 1. N applications increased by 1.5 and 2 times with the fixed amount of $P_2O_5$ and $K_2O$ (8kg/10a each) increased the proportion absorbed to the applications of N in both non salty and low-salty areas. It was observed that the absorption of Ca and Si was inhibited by either an increased treatment of N alone or combination with the other nutrients in the salty area. 2. In the non-salty area, an increased applications of standard amount of N, $P_2O_5$ and $K_2O$ respectively did not increased the yields. Doubling the application of $K_2O$ resulted in a decreased yield. 3. Applications of additional of 1.5 and 2 times the 10 kg of N per 10a increased the rice yields 12% and 21% respectively, in the low-salty area. An increased application of $P_2O_5$ and $K_2O$ failed to bring about an increased yield. 4. Increasing the application of N gave a significant increased in the yield of rice grain and 1.5 times of N applications were seemed profitable on the high-salty area. Although an increased applications $P_2O_5$ and $K_2O$ seemed to increase the yields of grain, no significant increase was observed. 5. An increased application of N increased the number of panicles up to 1.5 times the standard amount in the non-salty area, but no further increase resulted by doubling the application. The number of panicles was increased in proportion to the increased application of N in both low and high-salty areas. An increased application of $P_2O_5$ increase the number of panicles per unit area in each experimental plot while that of $K_2O$ had no effect but rather decreased the number. 6. The effect of an increased application of N decreased the weight of panicle in the non-salty area, but when the application was increased to 1.5 times or more an increased weight of panicle resulted in both salty areas. Doubling the application had approximately the same effect as 1.5 times the application. Increasing the applications of $P_2O_5$ and $K_2O$ had no effect on the panicle weight in the experimental plots. Increasing the applications of N, $P_2O_5$ and $K_2O$ did not effect the weight of 1,000 grains produced in the non-salty and salty areas. Increasing the application of N decreased the number of grains per panicle in the non-salty area but increased the number of grains per panicle in either salty areas. 7. The ratio of matured grains was highest in the low-salty area and the lowest in the high-salty area. An increased N applications decreased the ratio of matured grains in the non-salty area. No effect was observed in both low and high-salty areas. Increased the $P_2O_5$ and $K_2O$ application showed no effect on the ratio of matured grains in the experimental plots. 8. Increased applications of N, $P_2O_5$ and $K_2O$ was observed not to change the percentage of milling recovery in any experimental plots. Broken rice was increased equally by an increased application of N in the non-salty and salty areas but more remarkably so in the former. 9. Increased applications of N increased the straw production equally in the non-salty, low and high-salty areas. However, no increased production was observed from heavier applications of $P_2O_5$ and $K_2O$. Additional N applications reduced the rate of rough grain weight v.s. straw weight in the non-salty area but increased the ratios in both low and high-salty areas. Additional $P_2O_5$ and $K_2O$ had no effect with the ratio.

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A Preliminary Study on Depressive Symptoms and Glycemic Controls in Diabetic Patients (당뇨병 환자에서의 우울 및 관련증상에 관한 예비적 연구)

  • Ko, Seung-Hyun;Jeong, Jong-Hyun;Hong, Seung-Chul;Han, Jin-Hee;Lee, Seung-Pil;Ahn, Yoo-Bae;Song, Ki-Ho
    • Korean Journal of Psychosomatic Medicine
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    • v.12 no.2
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    • pp.165-173
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    • 2004
  • Objectives: Diabetes mellitus is a heterogeneous, chronic, progressive disease characterized by hyperglycemia and abnormality in protein, carbohydrate, fat metabolism. Recent studies have reorted two times prevalence of depression in individuals with diabetes compared to individuals without diabetics. This study was designed to investigate glycemic controls, anxiety, alexithymia, stress responses between depressed diabetic patients and non-depressed diabetic patients. Methods The subjects were 60 diabetic patients(mean age : $50.3{\pm}9.7$ years, 31 men and 29 women) who were confirmed to have diabetes depending on the laboratory findings as welt as clinical symptoms at the St. Vincent Hospital Diabetes Clinic, from Mar. 2004 to Sep. 2004. Laboratory test including, blood chemistry. glycated hemoglobin, urinalysis for proteinuria and Korean version of Beck Depression Inventory(BDI), State and Trait Anxiety Inventory(STAI), Toronto Alexithymia Scale(TAS) and Stress Response Inventory(SRI) were used for assessment. Based on BDI scores, all diabetics were divided into 13 depressed-diabetics group(above 20 point) and 47 non-depressed group(below 20 point). We compared demographic data. glycemic controls, STAI, TAS and SRI scores between two groups by independent t-test. Results : 1) Depressed diabetic groups were 13(mean age : $55.4{\pm}7.2$ years, 7 men and 6 women) and non depressed groups were 47(mean age $48.9{\pm}9.8$ years, 24 men and 23 women). In depressed diabetics, compared with non-depressed group, manifested aged(p=0.031), but other demographic data showed no difference between two groups. 2) No significant differences were noted in FBS, PP2h, Hb A1C, total cholesterol, HDL-cholesterol, SGOT/SGPT, BUN levels between depressed and non-depressed groups. But, blood creatine levels of depressed group were significantly increased than non-depressed group(p=0.026). 3) No significant differences were found in the score of STAI, STAI-S, STAI-T, TAS between depressed and non-depressed groups. 4) The SRI scores of depressed groups were significantly higher than non-depressed groups$(59.7{\pm}24.9\;vs.\;31.5{\pm}22.0)(p=0.000)$. Conclusion : The above results suggest that depressed diabetic patients are have more stress responses and higher blood creatine levels. However, there were no differences in laboratory data related to glycemic controls, and anxiety. alexithymia levels between two groups. We suggest that physicians should consider integrated approaches for psychiatric problems in the management of diabetes.

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Investigation on Korean Local Maize Lines V. Variabilities of Plant Characters of Multi-eared and Tillered Lines(MET) (재래종 옥수수 수집종에 대한 특성조사 제5보 다수다벽 재래종 옥수수계통의 특성변이)

  • Choe, B.H.;Park, J.S.;Kim, Y.R.;Park, K.Y.
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.26 no.1
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    • pp.56-68
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    • 1981
  • A maize line was selected in 1979 among 1000 Korean local maize lines collected in 1977. The selected maize line was characterized by having three to four tillers and eight to 10 ears on each individual plant. The line was assumed to have a great potential as a silage crop. The investigation was conducted as one of the serial studies on the Korean maize collected lines to provide basic information on the genetic variabilities of the multi-eared and tillered (MET) line and on other agronomic characters, prior to use the line as material for future breeding works for silage crop. The MET line and Suwon #19, single cross hybrid, as check variety were planted on May 1, 15 and 30, in three different levels of plant populations. The results obtained were summarized as follows: 1. The genetic variabilities of multi-ear and tillering habits were greater than environmental variabilities. 2. Total dry leaf weight of individual plant of MET line was also significantly higher than that of Suwon #19. 3. The mean number of tillers and ears bearing on the individual plant of MET line varied greatly with plant densities. The number of tillers and ears was on the average 2.9 and 7.0, respectively, when planted in 60cm. by 60cm. 4. The total dry matter and dried stem weight of the individual plant on MET line were comparable to those of Suwon #19. 5. The kernel weight from the individual plant of MET line was 5 to 40% less than that of Suwon #19, depending upon the plant densities. 6. The Kernel to stover ratio was higher for Suwon #19 than for the MET line. (41% to 35%). 7. The MET line had shown first tiller two weeks after planted on May 1. The second and third tillers appeared three to five days after the appearance of the first tiller. 8. The MET line was very specific in tillering habits. All the tillers were borne on the first few nodes of main stem below the soil surface. 9. The tillering habits of MET line were vigorous in the early part of the growing season, but less vigorous in the later part of the growing season. The number of efficient tillers bearing useable ears, was around two to three, when planted in 60cm. by 60cm. 10. The difference of plant height between main stem and first few tillers was around 10cm. 11. The ear size of MET line was around one-third of the major corn belt hybrids. The shape of ear of MET line was conical, with different diameter. 12. The kernel of the MET line was flinty with small soft starch patches on the endosperm part. 13. The 100 kernel weight was around 15gr., which is about one half of the major high yielding hybrids. 14. The ear height of MET line was comparatively higher than that of Suwon #19. 15. Significantly high and positive phenotypic correlation coefficients were obtained among major plant characters. 16. The growth rate of MET line was slower than that of Suwon #19. 17. MET line and Suwon #19 were both heavily infected with black streaked mosaic virus.

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Seeding Rate and Planting Date Effects on Forage Performance and Quality of Winter Rye (호밀의 사초특성, 수량 및 품질에 미치는 파종량 및 파종기의 영향)

  • Park, H.S.;Kim, D.A.;Kim, J.D.
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.19 no.2
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    • pp.105-114
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    • 1999
  • This experiment was carried out to determine seeding rate and planting date effects on the forage performance and quality of winter rye(Secale cereale L.) at Suweon in 1997 and 1998. The experiment was arranged in a spilt plot design with three replications. Main plots consisted of three seeding rates; 100, 150, and 200kg/ha. Sub-plots consisted of four planting dates; 5 September, 20 September, 5 October and 20 October. The first heading date of rye was not strongly influenced by planting dates. On a day basis, a 1:15 ratio was existed between heading and planting dates of rye, as a 1-day delay in spring heading date for each 15-day delay in fall planting date. Dry matter content of rye for a seeding rate of 200kg/ha was the lowest of 13.6%, and that for the planting dates was decreased to 15.2, 14.2, 14.3, and 13.8% with delayed seeding (P<0.05). There was an interaction between seeding rate and planting date in dry matter content of rye(P<0.01). Acid detergent fiber (ADF) percentage of rye for the seeding rates was not significant and that for the planting dates was decreased to 32.1, 31.6, 31.6, and 29.3%, as the planting was delayed(P<0.05). There was an interaction between seeding rate and planting date for ADF. Effect of seeding rate and planting date on neutral detergent fiber(NDF) of rye was similar to the observations made on ADF. Crude protein content of rye for the seeding rates was not significant, but that for the planting dates was increased to 17.3, 17.7, 18.2, and 18.9%, as the planting was delayed(P<0.05). In vitro dry matter digestibility(IVDMD) of rye for the seeding rates was not significant, but that for the planting dates was increased to 77.5, 80.6, 80.9, and 80.9%, as the planting was delayed(P<0.05). Dry matter yield of rye for a seeding rate of 100 kg/ha was the highest of 9,059 kg/ha, and that for a seeding rate of 200 kg/ha was the lowest of 7,647 kg/ha(P<0.01). In this experiment, the highest forage yield(8,945 kg/ha) was obtained when planting was completed by early October(5 October), with yield decreased as planting was delayed until 20 October (7,249 kg/ha)(P<0.01). This trend was also observed for the crude protein(CP) and in vitro digestible dry matter(IVDDM) yields of rye. A significant interaction between seeding rate and planting date for the dry matter yield was occurred(P<0.01). Based on the results of this experiment, it appears that the forage dry matter yield of rye could be enhanced by sowing from 20 September to 5 October under upland condition in the middle plain area of Korea. The seeding rates from 100 to 150 kg/ha and that of 200 kg/ha would be suitable for the early-fall and late fall sowing, respectively.

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The Role of Intraluminal Brachytherapy in Management of Esophageal Cancer (식도암 치료에 있어 관내근접치료의 역할)

  • Lee Chang Geol;Suh Chang Ok;Kim Gwi Eon;Chu Sung Sil;Chung Eun Ji;Kim Woo Cheol
    • Radiation Oncology Journal
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    • v.13 no.4
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    • pp.331-338
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    • 1995
  • Purpose : To evaluate our clinical experience with the combination of teletherapy and intraluminal brachytherapy in patients with unresectable or inoperable esophageal cancers. Materials and Methods : From Nov 1989 to Mar 1993, twenty patients with esophageal cancer were treated with radical radiotherapy and intraluminal brachytherapy at Yonsei Cancer Center. All patients had squamous histolgy and stage distribution was as follows: stage II, 4($20{\%}$)patients; III, 15 ($75{\%}$)patients; IV, 1($5{\%}$)patients. A dose of S-12Gy/1-3weeks with intraluminal brachytherapy (3-5Gy/fraction) to 5mm from the outside of the esophageal tube using high dose rate Iridium-192 remotely afterloading brachytherapy machine was given 2 weeks after a total dose of 59-64Gy with external radiotherapy. Induction chemotherapy using cisplatin and 5-FU was performed in 13 patients with median 3 cycles(1-6 cycles), Response rate, local control rate, survival and complications were analysed retrospectively. Results : Two-year overall survival rate and median survival were $15.8{\%}$ and 13.5 months. Response rates were as follows complete remission(CR) 5($25{\%}$): partial remission a(PRa) 7($35{\%}$): partial remission b(PRb) 7($35{\%}$), no response(NR) 1($5{\%}$). Patterns of failure were as follows; local failure 13($65{\%}$), local and distant failure 3($15{\%}$), distant failure 0($0{\%}$). Ultimate local control rate was $20{\%}$. Treatment related complications included esophageal ulcer in two patients and esophageal stricture in one. Conclusion : Though poor local conrol rate, median survival was improved as compared with previous results of radiation therapy alone(8months) and chemoradiation combined treatment(11 months) in Yonsei Cancer Center High-dose-rate intraluminal brachytherapy following external irradiation is an effective treatment modality with acceptable toxicity in esophageal cancer.

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Risk Factors for Mortality in Community-Acquired Pneumonia Patients Admitted to a Referral Hospital (지역사회획득폐렴으로 대학 병원에 입원한 성인의 사망률과 관련된 위험인자)

  • Lee, Young Woo;Jung, Jae Woo;Song, Ju Han;Jeon, Eun Ju;Choi, Jae Cheol;Shin, Jong Wook;Kim, Jae Yeol;Park, In Won;Choo, Byoung Whui
    • Tuberculosis and Respiratory Diseases
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    • v.61 no.4
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    • pp.347-355
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    • 2006
  • Background: Pneumonia is the most common cause of death among infectious diseases with community-acquired pneumonia being the sixth leading cause of death in the USA. In Korea, several studies have evaluated the prognosis of community-acquired pneumonia with a limited number of patients and risk factors. This study, evaluated all the possible risk factors (including the pneumonia severity index; PSI) in for the community-acquired pneumonia patients admitted to a referral hospital. Methods: The medical records of patients admitted to the Chung-Aug University Yongsan Hospital between January 2002 and January 2005 for community-acquired pneumonia were reviewed retrospectively. The demographic data, comorbidity, radiographic findings and laboratory results which might influence the prognosis of pneumonia were analyzed. Results: Among 179 patients admitted for community-acquired pneumonia, 29 patients died (mortality 16%). The risk factors for mortality in the comorbidity category were congestive heart failure and a myocardial infarction. The laboratory data, showed that albumin, LDH, total cholesterol, HDL, PT, aPTT, hemoglobin and blood urea nitrogen (BUN) were related to the prognosis. For the pneumonia severity index, the mortality rate increased in a step-wise manner from class I through class V. Conclusions: Comorbidities such as congestive heart failure and myocardial infarction as well as the albumin, LDH, total cholesterol, HDL cholestreol, prothrombin time, activated partial thrombotin time, hemoglobin and blood urea nitrogen(BUN) are important risk factors for mortality in patients with community-acquired pneumonia. PSI is a valuable index for evaluating the prognosis of community-acquired pneumonia.

Effects of Polymorphisms in the 3' Untranslated Region of the Porcine Mitochondrial calcium uptake 1 (MICU1) Gene on Meat Quality Traits (돼지 mitochondrial calcium uptake 1 (MICU1) 유전자의 3'UTR 내 SNP가 육질에 미치는 영향)

  • Jee, Yae-Sol;Cho, Eun-Seok;Jeon, Hyeon-Jeong;Lee, Si-Woo;Lim, Kyu-Sang;Kim, Tae-Hun;Lee, Kyung-Tai
    • Journal of Life Science
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    • v.26 no.11
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    • pp.1232-1236
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    • 2016
  • Mitochondrial calcium uptake 1 (MICU1) including two canonical EF hands, located in the mitochondrial inner membrane, is known to play a crucial role in the calcium uptake in mitochondria. Mitochondrial calcium uptake in muscular cells is related to post mortem shortening by calcium release from muscles. Therefore, the porcine MICU1 gene has been estimated as a genetic candidate for meat quality traits. In this study, variations on the exonic regions of the porcine MICU1 gene were investigated by sequencing cDNAs and tested for their association with meat quality traits. A total of 667 Berkshire heads (347 sows and 320 castrated boars) were used for this association test. Three SNPs were detected on the 3' untranslated region (UTR) of the porcine MICU1 gene. SNP1 (c.*136G>A) was associated with drip loss (p=0.017) and intramuscular fat content (p=0.039). In addition, SNP2 (c.*222G>A) and SNP3 (c.*485G>A) were associated with drip loss (p=0.018) and intramuscular fat content (p<0.001), respectively. In conclusion, it was verified that three variations on the 3' UTR of the porcine MICU1 gene were significantly associated with meat quality traits. It was also suggested that molecular biological analyses are needed to validate the function of variations on the 3 UTR of the porcine MICU1 gene.

Epidemiologic Investigation for the Etiology of an Epidemic Ocurred among Animals and Humans in an Isolated Island, Korea(I) (신안군(新安郡) 낙도(落島)에서 발생(發生)한 괴질(怪疾)의 원인(原因)에 관한 역학적(疫學的) 조사(調査)(I))

  • Kim, J.S.;Heo, Y.;Yoon, H.Y.;Lee, W.Y.
    • Journal of Preventive Medicine and Public Health
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    • v.22 no.2 s.26
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    • pp.290-301
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    • 1989
  • This is preliminary report on anthrax epidemic occurred in an island with about 100 residents. Since 1982 there had been sudden deaths among all kinds of domestic animals including cattle, dogs, ducks, chicken and goat but only a few among cats in an isolated island about three hours distance away by ferry boat from Mokpo city. From 1986 through 1988 nine human deaths and four patients occurred, which made the government intervene for investigation on June 25 1988. The epidemiological investigation consisted of interview survey and medical examination, medical record analysis, laboratory work to isolate the pathogens under the direction of hypothesis derived from the study and further confirmation of the pathogens by international institute. The summarized results are as followings: 1. According to the interview survey there were many deaths among domestic animals usually in cold and dry season such as January through March and September through November; 36 heads of cattle leaving one head, more than 40 hogs(all), hundreds of chicken leaving few alive, goats that had taken home from mountain and two or three cats out of around 40 had sudden deaths from 1982 till 1985, when the residents stopped to purchase and take them into the island anymore. Also there were eleven persons who had experienced the similar syndrome complex to those of admitted and expired patients and four of them revealed typical chest X-ray findings; from one of these four patients(Rho) B. anthracis is isolated. 2. Medical record on patients who had been admitted, showed common characteristics of the disease course. On admission they had either gastrointestinal or upper respiratory infection symptoms which invariably progressed to septicemic nature with pulmonary interstitial infiltration and mediastinal widening/bulging, and then to deadly acute respiratory distress syndrome. At the end stage chest X-ray revealed multiple bullous emphysema. One of another characteristics was oral ulceration with bleeding occurred in about 50% of the patients. Laboratory test results in common were leukocytosis with left shift and abnormal liver and kidney functions, particularly at the later stage of the illness. 3. Epidemiological characteristics was striking in that both mortality and incidence rates were high: the mortality rate was 8.7% average, male being three times higher than females but there was no distictive clustering by age group. The incidence rate for both sexes was 28.2% and there was no sex difference although a tendency of higher incidence among older ages was noticed. The highest mortality and incidence were observed in Won village where the first death of animal occurred and with the highest frequency among three villages of the island. 4. Among twelve bacilli species isolated from various specimens, two strains, one from patient and the other from soil where the recently died cow is hurried, were confirmed as B. anthracis by Pasteur Institute and CDC of USA(strain from soil). CDC reported that the strain did not produce capsule in bicarbonate media but reacted with the bacteriophage and one of five sera taken from the patients. Mode of transmission as well as incubation period of the agent has not been established yet, which needs further investigation in relation to the antigenic structure of the variant when it is confirmed.

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The Characteristics and Genesis of Terrace Soils in Yeongnam Area -I. Macro-Morphological Features and Soil Profile Development Index of the Terrace Soils (영남지역(嶺南地域)에 분포(分布)된 단구지토양(段丘地土壤)의 특성(特性)과 생성연구(生成硏究) -제(第) 1 보(報) 단구지토양(段丘地土壤)의 형태적특성(形態的特性)과 토양단면발달도(土壤斷面發達度))

  • Jung, Y.T.;Um, K.T.;Ha, H.S.
    • Korean Journal of Soil Science and Fertilizer
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    • v.18 no.2
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    • pp.177-188
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    • 1985
  • To clarify the characteristics and genesis of clayey terrace soils in Yeongnam area, macro-morphological features and soil profile development indexes were investigated with the sequential soils in Yeongcheon (inland region) and in Yeongil (coastal region). The results are summarized as follows; 1. According to the physiogaphy and landuse, the terrace soils were discernible to well drained yellowish red (5 YR) profile of higher terrace (Bancheon series), moderately well drained reddish yellow (7.5 YR) profile of middle terraces (Upyeong and Hwadong series), and lower terraces (Deogpyeong and Geugrag series) which were moderately well to imperfectly drained by paddy-fication. 2. The roundness and sphericity of the gravels contained in the terrace deposits were ranged around 0.543-0.546 and 0.723-0.722, respectively. The rounded to well rounded gravels were resemble to typical alluvial origins. 3. The amount of clay minerals formed in the soil horizons per 100g of parent materials were 50.8-30.7g while the rates of the clay leached were 30.1-7.4%, and the higher terrace had the more leaching rates. 4. The index of profile development of the terrace soils ranged from 37.95 to 22.01 and the index were positively correlated with relative elevations of the soils. The rates of clay leaching were positively correlated with the ratios of clay in the illuvial horizons to elluvial horizons. 5. A similarity was observed among cumulative grain size curves of the terrace soils, but the patterns of recalculated silt free cumulative grain size curve of terrace deposits in Upyeong soils in Yeongil area were abruptly different from down layer that suggest the soil to have bisequum profile.

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Development of a Predictive Model Describing the Growth of Listeria Monocytogenes in Fresh Cut Vegetable (샐러드용 신선 채소에서의 Listerio monocytogenes 성장예측모델 개발)

  • Cho, Joon-Il;Lee, Soon-Ho;Lim, Ji-Su;Kwak, Hyo-Sun;Hwang, In-Gyun
    • Journal of Food Hygiene and Safety
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    • v.26 no.1
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    • pp.25-30
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    • 2011
  • In this study, predictive mathematical models were developed to predict the kinetics of Listeria monocytogenes growth in the mixed fresh-cut vegetables, which is the most popular ready-to-eat food in the world, as a function of temperature (4, 10, 20 and $30^{\circ}C$). At the specified storage temperatures, the primary growth curve fit well ($r^2$=0.916~0.981) with a Gompertz and Baranyi equation to determine the specific growth rate (SGR). The Polynomial model for natural logarithm transformation of the SGR as a function of temperature was obtained by nonlinear regression (Prism, version 4.0, GraphPad Software). As the storage temperature decreased from $30^{\circ}C$ to $4^{\circ}C$, the SGR decreased, respectively. Polynomial model was identified as appropriate secondary model for SGR on the basis of most statistical indices such as mean square error (MSE=0.002718 by Gompertz, 0.055186 by Baranyi), bias factor (Bf=1.050084 by Gompertz, 1.931472 by Baranyi) and accuracy factor (Af=1.160767 by Gompertz, 2.137181 by Baranyi). Results indicate L. monocytogenes growth was affected by temperature mainly, and equation was developed by Gompertz model (-0.1606+$0.0574^*Temp$+$0.0009^*Temp^*Temp$) was more effective than equation was developed by Baranyi model (0.3502-$0.0496^*Temp$+$0.0022^*Temp^*Temp$) for specific growth rate prediction of L.monocytogenes in the mixed fresh-cut vegetables.