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http://dx.doi.org/10.5352/JLS.2016.26.11.1232

Effects of Polymorphisms in the 3' Untranslated Region of the Porcine Mitochondrial calcium uptake 1 (MICU1) Gene on Meat Quality Traits  

Jee, Yae-Sol (Animal Genomics and Bioinformatics Division, National Institute of Animal Science, Rural Development Administration)
Cho, Eun-Seok (Swine Science Division, National Institute of Animal Science, Rural Development Administration)
Jeon, Hyeon-Jeong (Animal Genomics and Bioinformatics Division, National Institute of Animal Science, Rural Development Administration)
Lee, Si-Woo (Animal Genomics and Bioinformatics Division, National Institute of Animal Science, Rural Development Administration)
Lim, Kyu-Sang (Animal Genomics and Bioinformatics Division, National Institute of Animal Science, Rural Development Administration)
Kim, Tae-Hun (Animal Genomics and Bioinformatics Division, National Institute of Animal Science, Rural Development Administration)
Lee, Kyung-Tai (Animal Genomics and Bioinformatics Division, National Institute of Animal Science, Rural Development Administration)
Publication Information
Journal of Life Science / v.26, no.11, 2016 , pp. 1232-1236 More about this Journal
Abstract
Mitochondrial calcium uptake 1 (MICU1) including two canonical EF hands, located in the mitochondrial inner membrane, is known to play a crucial role in the calcium uptake in mitochondria. Mitochondrial calcium uptake in muscular cells is related to post mortem shortening by calcium release from muscles. Therefore, the porcine MICU1 gene has been estimated as a genetic candidate for meat quality traits. In this study, variations on the exonic regions of the porcine MICU1 gene were investigated by sequencing cDNAs and tested for their association with meat quality traits. A total of 667 Berkshire heads (347 sows and 320 castrated boars) were used for this association test. Three SNPs were detected on the 3' untranslated region (UTR) of the porcine MICU1 gene. SNP1 (c.*136G>A) was associated with drip loss (p=0.017) and intramuscular fat content (p=0.039). In addition, SNP2 (c.*222G>A) and SNP3 (c.*485G>A) were associated with drip loss (p=0.018) and intramuscular fat content (p<0.001), respectively. In conclusion, it was verified that three variations on the 3' UTR of the porcine MICU1 gene were significantly associated with meat quality traits. It was also suggested that molecular biological analyses are needed to validate the function of variations on the 3 UTR of the porcine MICU1 gene.
Keywords
Drip loss; intramuscular fat content; MICU1 gene; pig;
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1 Berridge, M. J., Bootman, M. D. and Roderick, H. L. 2003. Calcium signalling: dynamics, homeostasis and remodelling. Nat. Rev. Mol. Cell Biol. 4, 517-529.
2 Bowker, B. C., Grant, A. L., Forrest, J. C. and Gerrard, D. E. 2000. Muscle metabolism and PSE pork. Proceedings of the American Society of Animal Science.
3 Collins, S. and Meyer, T. 2010. Cell biology: A sensor for calcium uptake. Nature 467, 283.   DOI
4 De Stefani, D., Raffaello, A., Teardo, E., Szabo, I. and Rizzuto, R. 2011. A forty-kilodalton protein of the inner membrane is the mitochondrial calcium uniporter. Nature 476, 336-340.   DOI
5 Fujii, J., Otsu, K., Zorzato, F., de Leon, S., Khanna, V. K., Weiler, J. E., O'Brien, P. J. and MacLennan, D. H. 1991. Identification of a mutation in porcine ryanodine receptor associated with malignant hyperthermia. Science 253, 448-451.   DOI
6 Garcia-Esteban, M., Ansorena, D., Gimeno, O. and Astiasaran, I. 2003. Optimization of instrumental colour analysis in dry-cured ham. Meat Sci. 63, 287-292.   DOI
7 Perocchi, F., Gohil, V. M., Girgis, H. S., Bao X. R., McCombs J. E., Palmer A. E. and Mootha, V. K. 2010. MICU1 encodes a mitochondrial EF hand protein required for Ca2+ uptake. Nature 467, 291-296.   DOI
8 Kozomara, A. and Griffiths-Jones, S. 2014. miRBase: annotating high confidence microRNAs using deep sequencing data. Nucleic Acids Res. 42, D68-73.   DOI
9 Li, X., Yang, S., Tang, Z., Li, K., Rothschild, M. F., Liu, B. and Fan, B. 2014. Genome-wide scans to detect positive selection in Large White and Tongcheng pigs. Anim. Genet. 45, 329-339.   DOI
10 McLaren, W., Gil, L., Hunt, S. E., Riat, H. S., Ritchie, G. R., Thormann, A., Flicek, P. and Cunningham, F. 2016. The ensembl variant effect predictor. Genome Biol. 17, 122.   DOI
11 Wang, L., Yang X., Li, S., Wang, Z., Liu, Y., Feng, J., Zhu, Y. and Shen, Y. 2014. Structural and mechanistic insights into MICU1 regulation of mitochondrial calcium uptake. EMBO J. 33, 594-604.   DOI
12 Wang, X. J., Reyes, J. L., Chua, N. H. and Gaasterland, T. 2004. Prediction and identification of Arabidopsis thaliana microRNAs and their mRNA targets. Genome Biol. 5, R65.   DOI