• Nuclear Medicine and Molecular Imaging
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• v.42 no.5
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• pp.394-400
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• 2008

Purpose: Quantitative comparison of transgene expression within stem cells between lentivirus and adenovirus-mediated delivery systems has not been reported. Here, we evaluated the human sodium iodide symporter (hNIS) gene expression in rat mesenchymal stem cell (rMSC) transduced by lentivirus or adenovirus, and compared the hNIS expression quantitatively between the two delivery systems. Materials and Methods: Lentiviral-mediated hNIS expressing rMSC (lenti-hNIS-rMSC) was constructed by cloning hNIS gene into pLenti6/UbC/V5-DEST (Invitrogen) to obtain pLenti-hNIS, transducing rMSC with the pLenti-hNIS, and selecting with blasticidin for 3 weeks. Recombinant adenovirus expressing hNIS gene (Rad-hNIS) was produced by homologous recombination and transduction efficiency of Rad-hNIS into rMSC evaluated by Rad-GFP was $19.1{\pm}4.7%$, $54.0{\pm}6.4%$, $85.7{\pm}8.7%$, and $98.4{\pm}1.3%$ at MOI 1, 5, 20, and 100, respectively. The hNIS expressions in lenti-hNIS-rMSC or adeno-hNIS-rMSC were assessed by immunocytochemistry, western blot, and 1-125 uptake. Results: Immunocytochemistry and western blot analyses revealed that hNIS expressions in lenti-hNIS-rMSC were greater than those in adeno-hNIS-rMSC at MOI 20 but lower than at MOI 50. However in vitro 1-125 uptake test demonstrated that iodide uptake in lenti-hNIS-rMSC ($29,704{\pm}6,659\; picomole/10^6\;cells$) was greater than that in adeno-hNIS-rMSC at MOI 100 ($6,168{\pm}2,134\;picomole/10^6\;cells$). Conclusion: Despite lower amount of expressed protein, hNIS function in rMSC was greater by lentivirus than by adenovirus mediated expression. Stem cell tracking using hNIS as a reporter gene should be conducted in consideration of relative vector efficiency for transgene expression.

• Journal of Practical Agriculture & Fisheries Research
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• v.13 no.1
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• pp.131-136
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• 2011

For the establishment of seed production technique of warm water abalone species Haliotis sieboldii, development of the fertilized eggs and its biological minimum temperature were determined. The durations of each development stages at the six rearing temperature regimes were expressed as an exponential equation: 4 celled stage 1/h = 0.1346T - 2.1709(r² = 0.88) Morula stage 1/h = 0.0176T - 0.2184 (r² = 0.89) Trochophore 1/h = 0.0063T - 0.0512 (r² = 0.98) Veliger 1/h = 0.0045T - 0.0295 (r² = 0.99) 2nd c.t. 1/h = 0.0008T - 0.0047 (r² = 0.99) According to the equation, the biological minimum temperature for Haliotis sieboldii was estimated to be 9.7 ℃.

• The Pure and Applied Mathematics
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• v.10 no.3
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• pp.185-192
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• 2003

It is shown that every almost linear mapping $h{\;}:{\;}X{\;}{\rightarrow}{\;}Y$ of a complex normed space X to a complex normed space Y is a linen. mapping when h(rx) = rh(x) (r > 0,$r\;{\neq}\;1$$x{\;}{\in}{\;}X$, that every almost quadratic mapping $h{\;}:{\;}X{\;}{\rightarrow}{\;}Y$ of a complex normed space X to a complex normed space Y is a quadratic mapping when $h(rx){\;}={\;}r^2h(x){\;}(r{\;}>{\;}0,r\;{\neq}\;1)$ holds for all $x{\;}{\in}{\;}X$, and that every almost cubic mapping $h{\;}:{\;}X{\;}{\rightarrow}{\;}Y$ of a complex normed space X to a complex normed space Y is a cubic mapping when $h(rx){\;}={\;}r^3h(x){\;}(r{\;}>{\;}0,r\;{\neq}\;1)$ holds for all $x{\;}{\in}{\;}X$.

• Bulletin of the Korean Chemical Society
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• v.18 no.3
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• pp.305-310
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• 1997

The synthesis and characterization of the platinum heterocyclic carboxaldehyde thiosemicarbazone complexes [NC5H4CRNNC(S)NHR'PtCl] (R=H, R'=CH3(1); R=CH3, R'=CH3(2); R=CH3, R=H(3)) and diphenylphosphinophenyl carboxaldehyde thiosemicarbazone complexes [Ph2PC6H4CHNNC(S)NHRPtCl] (R=CH3(5); R=iC3H7(6); R=Ph(7)) are described. Compounds 1-3 were prepared by reaction of Pt(SEt2)2Cl2 with 2-acetylpyridine-4-alkylthiosemicarbazone in the presence of NEt3. Compounds 5-7 were prepared using Pt(SEt2)2Cl2 in toluene with diphenylphosphinophenyl carboxaldehyde alkylthiosemicarbazone. The compounds have been characterized by microanalysis, NMR (1H, 13C, 31P) spectroscopy, and single-crystal X-ray diffraction. X-ray single crystal diffraction analysis reveals that compound 5 is a mononuclear platinum compound with P,N,S-coordination mode.

• Korean Journal of Plant Resources
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• v.24 no.4
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• pp.445-455
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• 2011

This study was performed to develop a natural preservative which had the most successful effect on prolonging the vase life of cut flowers Rosa hybrida 'Enjoy'. To determine the effect of sucrose on vase life of cut roses, R. hybrida 'Enjoy', 0, 1, 3 or 5% of sucrose was mixed with Hypnum jutlandicum extract. The result showed that in the case of adding sucrose to extracts from H. jutlandicum, vase life of cut flowers was shortened, along with the cut flowers losing chlorophyll, becoming decolorized and finally showing wilting phenomenon. However, using hydroxy quinoline sulfate (HQS) and extracts from H. jutlandicum together exhibited an improved effect of prolonging vase life, especially when adding 100 $mg{\cdot}L^{-1}$ HQS, which had the effect of prolonging the vase life of R. hybrida 'Enjoy' the most. Regarding pH of extracts, 2 $mg{\cdot}L^{-1}$ of extracts from H. jutlandicum show originally pH 3.8. This was compared with adjusted extract solutions as pH 3.0, 4.0 or 5.0. The results showed that the solution of pH 3.8 had the most successful effect on prolonging the vase life of R. hybrida 'Enjoy'. Given all the results, H. jutlandicum extract (2 $mg{\cdot}L^{-1}$) and HQS (100 $mg{\cdot}L^{-1}$) was the most effective in prolonging the vase life of cut R. hybrida 'Enjoy'. Moss is expected to be worth developing as a natural preservative since it is easy to get and causes no damage to the environment.

• Journal of the Korean Chemical Society
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• v.43 no.2
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• pp.141-149
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• 1999

The correlation was investigated between the observed heat of ligation and calculated quantum chemical quantities for octahedral $[M(H_2O)_{6-x}(NH_3)_x]^{2+} (M=Fe(II),\;Ni(II))$ complexes by EHMO(Extended Huckel Molecular Orbital) and ZINDO/1(Zerner's Intermediate Neglected of Differential Overlap)method. The net charge of $Fe^{2+}$ and $Ni^{2+}$ ion of octahedral $[M(H_2O)_{6-x}(NH_3)_x]^{2+}(M=Fe(II),\;Ni(II))$ complexes(x=O, 1, …, 6) decreased with substituting $NH_3$ for $H_2O$ molecules. It has found that a good correlation exists between the observed heat of ligation and the calculated quantum chemical quantities such as net charge of central atom, enthalpy of formation, and total dissociation energy. From this finding, we have obtained the following semiempirical linear equation ${\Delta}H_{obs}=-0.2858_{qFe}+0.8813(r=0.97),\;{\Delta}H_{obs}=-0.8981_{qNi}+1.7929(r=0.95),\;{\Delta}H_{obs}=-0.0031H_{f(Fe)}+0.5725(r=0.97),\;{\Delta}H_{obs}=-0.0095H_{f(Ni)}+0.9193(r=0.97),\;{\Delta}H_{obs}=0.0476E_{diss(Fe)}+0.6434(r=0.94),\;{\Delta}H_{obs}=0.1401E_{diss(Ni)}+1.1393(r=0.93)$.

• Industry Promotion Research
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• v.3 no.2
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• pp.1-8
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• 2018

In this study, the reaction mechanism of ethane and the reaction rate equation suitable for hydrocarbon reforming were studied. Through the reaction mechanism analysis, it was confirmed that three reactions (CO2 + H2, C2H6 + H2, C2H6 + H2O) proceed during the reforming reaction of ethane, each reaction rate (CO2+H2($r=3.42{\times}10-5molgcat.-1\;s-1$), C2H6+H2($r=3.18{\times}10-5mol\;gcat.-1s-1$), C2H6+H2O($r=1.84{\times}10-5mol\;gcat.-1s-1$)) was determined. It was confirmed that the C2H6 + H2O reaction was a rate determining step (RDS). And the reaction equation of this reaction can be expressed as r = kS * (KAKBPC2H6PH2O) / (1 + KAPC2H6 + KBPH2O) (KA = 2.052, KB = 6.384, $kS=0.189{\times}10-2$) through the Langmuir-Hinshelwood model. The obtained equation was compared with the derived power rate law without regard to the reaction mechanism and the power rate law was relatively similar fitting in the narrow concentration change region (about 2.5-4% of ethane, about 60-75% of water) It was confirmed that the LH model reaction equation based on the reaction mechanism shows a similar value to the experimental value in the wide concentration change region.

• Journal of the Korean Chemical Society
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• v.53 no.5
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• pp.530-535
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• 2009

Dicyclohexyl telluride was obtained in a high yield by the reaction of cyclohexyl bromide with NaTeH(prepared in situ) in an aqueous ethanolic solution. A series of new organotelluronium salts of the general formula ${(cyclo-C_6H_11)}_2Te(R)X$ (where R = $CH_3$, X = I (1); R = $C_2H_5$, X = Br(2); R = $C_2H_5$, X = I (3); R = C_3H_5$, X = Br (4)) were prepared by the reaction of ${(cyclo-C_6H_11)}_2Te$ with the corresponding alkyl halide. Reaction of 1 with NaBPh4 gave compound 5 ( i.e. R = CH3, X = BPh4 ‒) in 78% yield. Reaction of ${(cyclo-C_6H_11)}_2Te$ with benzyl bromide and 4-bromophenacyl bromide gave unexpectedly dibenzylcyclohexyltelluronium bromide (6) and bis(4-bromophenacyl)cyclohexyltelluronium bromide (7), respectively. Reaction of 6 with NaBPh4 gave the corresponding tetraphenylborate derivative (8) in high yield. $^1H$ NMR studies revealed that in $CDCl_3$solution compound 1 eliminated alkyl halide. Conductivity, IR, $^1H\;and\;^{13}C$ NMR and thermal data for the new compounds are presented and discussed.

• Journal of Life Science
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• v.23 no.10
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• pp.1260-1266
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• 2013

The ribosomal DNA (rDNA) clusters of Hypsizygus marmoreus 3-10 and H. marmoreus 1-1 were analyzed in this study. The small subunit (SSU) and intergenic spacer 2 (IGS 2) was partially sequenced. The internal transcribed spacer 1 (ITS 1), 5.8S, internal transcribed spacer 2 (ITS 2), large subunit (LSU), intergenic spacer 1 (IGS 1), and 5S were completely sequenced. The rDNA clusters of H. marmoreus 3-10 and H. marmoreus 1-1 were 7,049 bp in length. The sequence of SSU rDNA, which corresponded to 18S rDNA, was 1,796 bp in length, and the sequence of LSU rDNA, which corresponded to 28S rDNA, was 3,348 bp in length. The ITS region that variable region and IGS region that non-transcribed spacer was 462 bp and 1,290 bp in length. The sequence of 5.8S rDNA and 5S rDNA was 153 bp and 43 bp in length, respectively. The 17 bp of the rDNA cluster in the H. marmoreus 3-10 strain was different to that in the H. marmoreus 1-1 strain, with 2 bp in the SSU, 3 bp in the ITS, 9 bp in the LSU, and 3 bp in the IGS. The analysis of the secondary structure revealed that the ITS regions of H. marmoreus 3-10 and H. marmoreus 1-1 have five stem-loop structures. Interestingly, among these structures, one different nucleotide sequence resulted in a different secondary structure in stem-loop V.

• Bulletin of the Korean Mathematical Society
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• v.57 no.3
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• pp.709-737
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• 2020

Let R be a prime ring of characteristic different from 2. Suppose that F, G, H and T are generalized derivations of R. Let U be the Utumi quotient ring of R and C be the center of U, called the extended centroid of R and let f(x₁, …, x_n) be a non central multilinear polynomial over C. If F(f(r₁, …, r_n))G(f(r₁, …, r_n)) - f(r₁, …, r_n)T(f(r₁, …, r_n)) = H(f(r₁, …, r_n)²) for all r₁, …, r_n ∈ R, then we describe all possible forms of F, G, H and T.